BACKGROUND: Nowadays, infertility is one of the major problems of human societies. OBJECTIVES: To study oral administration of bulk milk and milk of late pregnant cows on spermatogenesis of male rats. METHODS: The first group of rats from day 1 of pregnancy until the end of lactation and then their male pups to maturity were treated with late pregnant cow’s milk. The second group from day 12 of pregnancy up to 15 days after delivery was treated with late pregnant cow’s milk. The third group of rats from day 1 of pregnancy until the end of lactation and then their male pups to maturity were treated with bulk milk. The fourth group from day 12 of pregnancy up to 15 days after delivery was treated with bulk milk. Rats in the control group during the study period were only fed with special food of rats and at the end viability, types of movement (progressive and in-place movement, immobility), number of sperms and also the serum testosterone level were elevated. RESULTS: Administration of both types of milk had no effect on in-place movement and also viability of sperms of experimental groups but they could cause a significant increase in sperm  immobility and a significant decrease in number of sperms of experimental groups. Also,the level of serum testosterone of experimental groups was significantly reduced in comparison with control group (p<0.05). CONCLUSIONS: Overall, it was determined consumption of late pregnant cow’s milk and bulk milk when it contains high estrogen can cause changes in some sperm species that are involved in male reproduction.

Data from 34 yearling Hereford or Angus bulls were used to investigate relationships of testicular size, quantitative rates of sperm production, Sertoli cell numbers, numbers of germ cells supported per Sertoli cell, and the efficiency of spermatogenesis to daily sperm output and seminal quality. Two ejaculates were collected by electroejaculation from each bull on each of 2 days/week throughout the study. The percentage of progressively motile sperm and the percentage of morphologically normal sperm were determined from aliquots of fresh semen. Additional aliquots of semen were frozen in glass ampules or plastic straws and subsequently evaluated for postthaw motility and percentage of sperm with intact acrosomes. Sertoli cell numbers, the numbers of germcells per Sertoli cell, and the efficiency of spermatogenesis were unrelated to the quality of fresh or frozen semen (P greater than 0.05). In first ejaculates, the numbers of sperm and motile sperm were related (P less than 0.05) to testicular parenchymal weight (r = 0.38, and 0.50), daily sperm production (r = 0.45 and 0.53), and spermatids per gram of testicular parenchyma (r = 0.35 and 0.34). Testicular parenchymal weight and daily sperm production also were related to daily sperm output and to the average daily motile sperm output of these bulls (P less than 0.05), but could account for less than 25% of the variability in these end points among bulls.

In the current study demonstrated the role of irradiation with stimulate the cells on division and accelerate of spermatogenesis in mice testis following low –level laser therapy and anti-inflammatory process. Twenty -eight healthy male and female mice .They were maintained in individual cages with free access to water and a pellet balanced commercial diet for mice . All animals have been weighing by using sensitive balance and the mean body of mice (25+_5 gm) . The stages of Q-S Switch Nd:YAG laser set-up with 632 nm 10 ns of pulse duration and 10 Hz of frequency to get a dose or energy density were : 1-set a single pulse on Q-Switch Nd:YAG laser using control panel with repetition rate of 10 Hz . Mice were irradiated to testes a 670 nm with intensity 4 J/cm2 /day .for seven days .But mice of control group without exposure any laser

The seminiferous tubules of the testes of rabbits were lined by multilayered germ cells . The first layer was occupied by the spermatogonia , which were differentiated into type A(dusty type) spermatogonia, Intermediate type and type B (crusty type) spermatogonia. Pictures of Preleptotene, Leptotene, Zygotene ,Pachytene, Diplotene and Diakinesis. Primary spermatocytes were found and followed by secondary spermatocytes. Reading the morphological changes, the spermatid proposed 10 stages of cellular association during the cycle of the seminiferous epithelium in rabbit .

Body and testis weights, serum luteinizing hormone, follicle-stimulating hormone, and prolactin values and volume fractions of Sertoli cells, spermatogonia, early and late primary spermatocytes, and round and long spermatids were evaluated in 70-day-old male rats treated orally with 20 mg of zearalenone/kg of body weight daily for 5 weeks. A significant (P < 0.05) increase in serum prolactin concentration was consistently observed during the 5 weeks of treatment with zearalenone. Significant changes were not observed in any of the other variables evaluated.

A sample of testicular parenchymal tissue, approximately 2 X 7 X 7 mm, was aseptically removed from 1 testis in each of 9 stallions on day 0. Slight to moderate hemorrhage from the tunica albuginea was observed in 8 stallions, but bleeding from the parenchyma was detected in only 2 stallions. Stallions were castrated 27 days later. Normal development of granulation tissue was evident at the biopsy site, but hematomas were not observed. In situ measurement of the widths of the right and left testes, total scrotal width, and evaluation of testicular echogenicity during ultrasonography were variables used to monitor changes in the testicular parenchyma from 14 days before biopsy through 27 days after biopsy. The control testis was consistently larger than the biopsied testis, except for day 3. Ultrasonography revealed signs of a localized change in the parenchyma of the biopsied testis in 4 stallions, but each lesion decreased in size by day 27. Tissues removed during biopsy enabled an excellent appraisal of spermatogenesis at that time. Detailed examinations of seminiferous tubules in the testes were performed to assess for damage to testicular function. At castration, samples were taken from 6 sites in each testis. Quantitative histologic evaluations of testicular tissues revealed low numbers of spherical spermatids and pachytene spermatocytes in biopsied testes, compared with control testes. It was concluded that there was a transitory increase in degeneration of preleptotene spermatocytes and B spermatogonia at the time of biopsy. A mild inflammatory response at the biopsy site in some testes was evidenced by an increased number of leukocytes at the biopsy site and at a dorsal site. Because damage was minimal and appeared to be transitory, it was concluded that the open method of biopsy does not greatly alter the process of spermatogenesis or function of the testis in stallions.

The present study was conducted on (40) white mice of approximately the same age (4-6 weeks) and body weight (23-25 gm) for the aim of observing the histopathological changes for male and female reproductive organs due to prolonged treatment (6 months) with anticancer chemotherapeutic agent namely methotrexate. Forty mice were divided into 4 groups (10 mice of each group 5 mice per sex). The first group (low or therapeutic dose group) was received 0.15 mg/kg B.W. The second group (intermediate dose group ) received 0.3 mg/kg B.W. The third group ( toxic dose group ) received 0.45 mg/kg B.W. the fourth group was a control group; it received 0.2 ml buffered physiological saline. All these groups injected intramuscularly, once weekly for 6 months. The results showed that methotrexate can cause suppression of spermatogenesis. In female, methotrexate can cause obvious pathological changes in uteri and ovaries such as reduced endometrial glands and ovarian follicles respectively