This study sought to determine if sheep suffer neurological symptoms when fed Acacia angustissima leaves, and whether an equivalent amount of 70% acetone extract would have the same effect. In addition, the study tried to determine if treatment of leaves with 70% acetone would destroy the activity of A. angustissima toxins, and whether extraction with 70% aqueous acetone extract would separate two components of a toxic system. Twenty-five Menz lambs were randomly assigned to one of five treatments (1) A angustissima leaves as half the diet, 2) dried extract (70% aqueous acetone) of the same quantity of leaves, 3) a corresponding amount of residues, 4) a recombination of the dried extract and dried residue, or (5) a control diet containing no A angustissima leaves or extract fractions. All animals fed the leaves and the recombined fractions died or were euthanized when they were observed to be dying of severe neurological derangement. None of the other animals showed any neurological signs of impairement. The results of this study indicate that healthy, well-fed sheep can be poisoned by A angustissima, that the toxins are not destroyed by acetone or oven drying, and that severe neurological intoxication requires two components, which can be separated by acetone extraction.

The aim of this study was to investigate the effects of vitamin C (vit C) and magnesium L-threonate (MgT) on the learning ability and memory of mice intoxicated with lead acetate.

Afeeding trial was conducted in layers for a period of8 weeks (23-30 weeks old) to evaluate the effect of chromium picolinate supplementation on egg yolk cholesterol and egg chromium content in layers.Atotal of (n=120) layers were randomly distributed in completely randomized design into 4 treatmentgroupseachwith2 replicates of 5chicks.The layers of treatment group T1(control)were provided water without chromiumwhile those of T2,T3andT4were provided water containing 200, 400 and 600 ppb chromium / litre respectively.At the end of feeding trial, six eggs from each replicate, on last three days were used for egg yolk cholesterol and egg chromium studies. The results indicated that the total cholesterol andLDL cholesterol content of eggs were significantly (P0.05) reduced and HDL-cholesterol content significantly increased in 400 and 600 ppb chromium supplemented group of layers. Whereas chromium content of eggs increased with increasing levels of chromium supplementation. It was concluded that organic chromium supplementation up to 600 ppb level through water may be advisable to reduce egg yolk total cholesterol and LDL- cholesterol and improved HDL-cholesterol and egg chromium content.

In order to examine whether iodine supplements may have caused a global decline in sperm concentrations during the past several decades, the synchronicity of the decline in mean sperm counts and the introduction of io-dine supplements was analyzed statistically. A positive correlation between the incidence of thyroid disease and sperm counts has been detected in Europe. In addition, it has been shown that sperm counts began falling around 1965 in the United States, 40years after iodine supplements were introduced. Mean sperm counts before and after 1965 were 111 10 sup(6)/ml and 70 10 sup(6)/ml, respec-tively, in calculations weighted by the number of subjects included in each in-dividual publication. The timing of the declines coincided with the introduc-tion of iodine supplements in the United States, France, and the United King-dom. The implications are that the global decline in sperm concentrations may be caused by iodine intake. Geographical variation in the types of sperm count decline also appears to be present.

A technology of application autolysis of fodder yeasts (AFY) for the production of products with higher protein content, digestible aminoacids and other nutritive and biologically active substances in comparison with the standard fodder yeasts (provit) was tested in the conditions of the Republic of Belarus by an example of three groups of swine. Animals of the first (control) group were fed with combined feed SK-26 with addition of 2% of provit. Animals of the second group were fed with combined feed SR-26with addition of 2% of AFY, the third group - with addition 4% of AFY. Provit and AFY were included into combined feed instead of excluded protein components in equivalent number. Research results showed that autolysis of fodder yeasts did not render negative influence on productivity of fattening stores. Average daily weight gain of stores was 637-674 g. Calculation of economic efficiency of combined feeding application of 2 and 4% of AFY showed that application of 2% AFY was more economically profitable

Studying of efficiency of application of a natural biological control agent BioLAD obtained as a result of cultivation of Fusarium sambucinum fungus in feeding of broiler chickens was realized in the conditions of the Republic of Belarus. There were studied 100 broiler chickens of Ross-308 cross. Broiler chickens of the first (control) group were fed with standard diet with PK-5-1. Broiler chickens of the experimental groups starting from one day old age till the end of the growing period (41 days old) were fed with feed supplement BioLAD in different doses: 0,25 ml/head (second group), 0,5 ml/head (third group), 1 ml/head (fourth group). Research results showed that natural biological control agent BioLAD in dose of 0,5 ml/head made it possible to increase chicken live weight on 11,2 %, viability - on 4,2%. Feeding charges per 1 kg of live weight gain were1,89 kg (second group), 1,81 kg (third group), 1,90 kg (fourth group). It made it possible to decrease the combined feed charges on 6,4% (second group), on 10,4% (third group), and on 5,9% (fourth group). Application of biological control agent BioLAD had an effect on poultry growth activity, promoted high level of chickens viability, increased the indices of general and local protection, stimulated metabolic processes

Research of the influence of biologically active preparations of natural origin on zoological indices and quality of animal products was studied by the example of their application in broiler chicken diets in the conditions of the Republic of Belarus. On the basis of realized research it was established, that application of biologically active substances immunoamplifier Alveozan, probiotic Dialakt, probiotic Bifidoflorin liquid, prebiotic Biofon and prebiotic Biofon AIL (produced in the Republic of Belarus) did not render negative effect on quality of animal products (all studied meat samples had high quality). The received results testify to necessity of complex application of the above-stated preparations. Application of biologically active preparations made it possible to increase livability of growing birds on 100%, average live weight increase from 1,9 up to 5,1%, daily average weight gain - from 1,9 up to 5,1%, improvement of feed conversion ratio - from 18,3 up to 21%. All studied biologically active preparations made it possible to restore the physiological environment in poultry intestines, normal flora, to suppress the growth of pathogenic microorganisms. The above mentioned preparations could be applied both with preventive, and with the medical purposes

There was studied the data on antagonistic activity of a new metabolic probiotic substance derived from the probiotic substance Diamixan. Research of antagonistic activity Diamixan has shown its high activity in the relation of the test-strains of microorganisms: Staphylococcus aureus ATSS 6538, Staphylococcus aureus 209R, Escherichia coli ATSS 71229, Pseudomonas aeruginosa ATSS 9017, Pseudomonas marcescens, Proteus mirabilis ATSS 14153, Bacillus subtilis ATSS 6633, Citrobacter freundii, Candida albicans ATSS 10231, Salmonella enteritidis R1991, Salmonella typhimurium ATSS 24853. In the presence of the preparation, dissolved 1:1, there was a full suppression of growth of all bacteria within 48 hours of cultivation at delution 1:2 and 1:4 the high antagonistic activity of the preparation as the majority of samples remains sterile remained. In the tabular form data on antagonistic activity of the preparation in delution 1:1; 1:2; 1:4 are presented. | Целью работы явилось изучение антагонистической активности метаболитного пробиотика диамиксан. Исследование антагонистической активности диамиксана показало его высокую активность в отношении тест-штаммов микроорганизмов: Staphylococcus aureus АТСС 6538, Staphylococcus aureus 209Р, Escherichia coli ATCC 71229, Pseudomonas aeruginosa ATCC 9017, Pseudomonas marcescens, Proteus mirabilis ATCC 14153, Bacillus subtilis ATCC 6633, Citrobacter freundii, Candida albicans ATCC 10231, Salmonella enteritidis P1991, Salmonella typhimurium ATCC 24853. В присутствии разведённого 1:1 препарата происходило полное подавление роста всех бактерий в течение 48 часов культивирования, при разведении 1:2 и 1:4 сохранялась высокая антагонистическая активность препарата, так как большинство образцов оставалось стерильными. В табличной форме представлены данные по антагонистической активности препарата в разведении 1:1; 1:2; 1:4.

In the conditions of the Republic of Belarus there were studied biochemical indexes of calf blood serum against the background of application of probiotic preparations Bilavet C. Results of research showed that administration of probiotic preparations from the first day till the six day and from the fourteenth day till the nineteenth day of calf life with the preventive purpose rendered the simulative influence on indexes of natural resistance, which were accompanied with the increasing of total protein quantity in blood on 2,9-5,9%, as well as the increasing of protein fractions, in particular, globulin-rich fraction on 4,2-6,4%. Administration of probiotic preparation Bilavet С to calves rendered the positive influence on blood biochemical indexes, promoted the uptaking and better accumulation of mineral substances: content of crude calcium, inorganic phosphorus, magnesium, iron increased on 15,3%, 10,5%, 13,2% and 3,6%, respectively.