Recombinant LipL32 and LipL41 outer membrane proteins of Leptospira interrogans serovar Canicola were produced, and used as a pooled antigen in enzyme-linked immunosorbent assay (ELISA) to detect leptospiral antibodies in bovine sera samples. The optimum concentration of the pooled antigen was found to be 50ng of each antigen per well by using known positive and negative cattle sera. Using a total of 500 bovine sera samples the sensitivity, specificity and accuracy of pooled antigen based ELISA as compared to microscopic agglutination test (MAT) were 100%, 88.1% and 91.6%, respectively. The results suggested that antigen in ELISA could be preferred for detection of all those cases, which might have remained undiagnosed by performing MAT.

This study aimed to evaluate a system that identifies cartilage turn over and/or degradation through measurement of a new keratan sulfate (KS) epitope concentration in equine sera. Blood samples were collected from 30 horses, 1 (n=15) and 2 year-olds (n=15). Serum samples were analyzed for an epitope of keratan sulfate by 1/20/5D4 (KS5D4) and new epitopes of keratan sulfate using high sensitive keratan sulfate (HSKS), measured by two respective enzyme-linked immunosorbant assays (ELISAs). There was no correlation in serum concentration of KS evaluated using 5D4 and HSKS. Age had no significant effect on concentrations of KS measured with KS5D4 while 1 year-old horses showed significantly higher amounts than 2 year-olds with HSKS. Results suggest that HSKS could detect early signs of cartilage metabolic changes.