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Efficacy of a pseudorabies virus vaccine based on deletion mutant strain 783 that does not express thymidine kinase and glycoprotein I.
1991
Oirschot J.T. van | Moormann R.J.M. | Berns A.J.M. | Gielkens A.L.J.
The vaccine efficacy of a genetically engineered deletion mutant strain of pseudorabies virus, strain 783, was compared with that of the conventionally attenuated Bartha strain. Strain 783 has deletions in the genes coding for glycoprotein I and thymidine kinase. In experiment 1, which had a 3-month interval between vaccination and challenge exposure, strain 783 protected pigs significantly (P < 0.05) better against virulent virus challenge exposure than did the Bartha strain. The growth of pigs vaccinated with strain 783 was not arrested, whereas that of pigs vaccinated with the Bartha strain was arrested for 7 days. Of 8 pigs given strain 783, 4 were fully protected against challenge exposure; none of the pigs given strain Bartha was fully protected. In experiment 2, which had a 3-week interval between vaccination and challenge exposure, the growth of pigs vaccinated with strain 783 was arrested for 3.5 days, whereas that of pigs vaccinated with the Bartha strain was arrested for 6 days. In experiment 3, pigs with moderate titer of maternal antibodies were vaccinated twice IM or once intranasally with either strain 783 or Bartha and were challenge-exposed 3 months after vaccination. Pigs given strain 783 twice IM were significantly (P < 0.05) better protected than were the other pigs. They had growth arrest of only 6 days, compared with 9 days for pigs of other groups, and shed less virus after challenge exposure. Results of this study indicate that the vaccine based on the deletion mutant strain 783 is more efficacious than is the Bartha strain of pseudorabies virus.
显示更多 [+] 显示较少 [-]Second-generation pseudorabies virus vaccine with deletions in thymidine kinase and glycoprotein genes.
1987
Kit S. | Sheppard M. | Ichimura H. | Kit M.
Stability of gamma-glutamyltransferase activity in calf sera after refrigerated or frozen storage.
1997
Muller F. | Tyler J.W. | Parish S.M. | Johnson K.A. | Krytenberg D.S. | Wilson L.K.
Cytotoxic effect of acyclovir on cultured mammalian cells to which herpesvirus thymidine kinase gene was introduced
1989
Tanabe, K. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Hiraoka, W. | Kuwabara, M. | Sato, F. | Narita, T. | Niikura, M.
Studies on serum creatine phosphokinase isoenzyme: Seven cases of tetraplegia [paralysis of limbs due to spondylosis (spinal disease)] in the dog [for diagnosis of spinal disease]
1983
Yasuda, J. | Too, K. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine)
Restriction fragment length polymorphism for the Yc subunit gene of rat liver glutathione S-transferase
1990
Sasaki, Y. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Hayashi, M. | Matsumoto, K. | Namioka, S.
Identification of novel alternative splicing variants within swine Setd8 gene and their high mRNA expression in testis 全文
2017
Yu, S. (Northwest A and F University, Shaanxi (China). College of Animal Science and Technology) | Chen, X. | Deng, Z. | Lan, X. | Pan, C.
