The present study was conducted to investigate the effects of boldenone undecylenate (BUL) on carcass traits and blood stress parameters in growing male rabbits. A total of 170 male rabbits comprising of three breeds namely New Zealand White (NZW; n=55), Californian (CAL; n=50), and Rex (RX; n=65) were taken for this study. The rabbits of each breed were divided into three groups viz., D0 (control), D1 provided with normal dose of BUL (at 4.4 mg/kg body weight), and D2 provided with BUL (at 8.8 mg/kg bwt). The rabbits were kept under temperature ranged from 28-32°C during the experimental period. Carcass traits of the rabbits were studied, and the blood parameters were measured by radioimmunoassay. Most of carcass traits, globulin and cholesterol levels in the serum were significantly improved for the injection of BUL at normal dose. Plasma corticosterone levels in normal dose injected rabbits were lowered by 27.21 and 15.25% as compared to controls and double dose, respectively. The effect of interaction between dose and breed was non-significant (P>0.05) on almost all carcass traits and blood parameters. In conclusion, BUL improves all carcass traits in male growing rabbits when injected with normal dose of BUL, with the exception of dressing-out%, and has a significant lowering effect on stress hormone (i.e., corticosterone), and increasing effect on serum total protein, globulin and cholesterol.DOI: http://dx.doi.org/10.5455/javar.2015.b64

The present study was conducted to investigate the effects of boldenone undecylenate (BUL) on carcass traits and blood stress parameters in growing male rabbits. A total of 170 male rabbits comprising of three breeds namely New Zealand White (NZW; n=55), Californian (CAL; n=50), and Rex (RX; n=65) were taken for this study. The rabbits of each breed were divided into three groups viz., D0 (control), D1 provided with normal dose of BUL (at 4.4 mg/kg body weight), and D2 provided with BUL (at 8.8 mg/kg bwt). The rabbits were kept under temperature ranged from 28-32 and deg;C during the experimental period. Carcass traits of the rabbits were studied, and the blood parameters were measured by radioimmunoassay. Most of carcass traits, globulin and cholesterol levels in the serum were significantly improved for the injection of BUL at normal dose. Plasma corticosterone levels in normal dose injected rabbits were lowered by 27.21 and 15.25% as compared to controls and double dose, respectively. The effect of interaction between dose and breed was non-significant (P>0.05) on almost all carcass traits and blood parameters. In conclusion, BUL improves all carcass traits in male growing rabbits when injected with normal dose of BUL, with the exception of dressing-out%, and has a significant lowering effect on stress hormone (i.e., corticosterone), and increasing effect on serum total protein, globulin and cholesterol. [J Adv Vet Anim Res 2015; 2(3.000): 263-270]

The objective of this study was to compare theefficacy of tulathromycin (TUL) with a combinationof florfenicol (FFC) and long-acting oxytetracycline(LAOTC) in the treatment of naturally occurringundifferentiated respiratory diseases in largenumbers of sheep. In this study, seven naturaloutbreaks of sheep pneumonia in Garmsar, Iran wereconsidered. From these outbreaks, 400 sheepexhibiting the signs of respiratory diseases wereselected, and the sheep were randomly divided intotwo equal groups. The first group was treated with asingle injection of TUL (dosed at 2.5 mg/kg bodyweight), and the second group was treated withconcurrent injections of FFC (dosed at 40 mg/kg bwt)and LAOTC (dosed at 20 mg/kg bwt). In the firstgroup, 186 (93%) sheep were found to be cured 5 daysafter the injection, and 14 (7%) sheep needed furthertreatment, of which 6 (3%) were cured, and 8 (4%)died. In the second group, 172 (86%) sheep were curedafter the injections, but 28 (14%) sheep needed furthertreatment, of which 10 (5%) were cured, and 18 (9%)died. This study revealed that TUL was moreefficacious as compared to the combined treatmentusing FFC and LAOTC. As the first report, this fieldtrial describes the successful treatment ofundifferentiated respiratory diseases in largenumbers of sheep. Thus, TUL can be used for thetreatment of undifferentiated respiratory diseases insheep.http://dx.doi.org/10.5455/javar.2015.b86

The objective of this study was to compare the efficacy of tulathromycin (TUL) with a combination of florfenicol (FFC) and long-acting oxytetracycline (LAOTC) in the treatment of naturally occurring undifferentiated respiratory diseases in large numbers of sheep. In this study, seven natural outbreaks of sheep pneumonia in Garmsar, Iran were considered. From these outbreaks, 400 sheep exhibiting the signs of respiratory diseases were selected, and the sheep were randomly divided into two equal groups. The first group was treated with a single injection of TUL (dosed at 2.5 mg/kg body weight), and the second group was treated with concurrent injections of FFC (dosed at 40 mg/kg bwt) and LAOTC (dosed at 20 mg/kg bwt). In the first group, 186 (93%) sheep were found to be cured 5 days after the injection, and 14 (7%) sheep needed further treatment, of which 6 (3%) were cured, and 8 (4%) died. In the second group, 172 (86%) sheep were cured after the injections, but 28 (14%) sheep needed further treatment, of which 10 (5%) were cured, and 18 (9%) died. This study revealed that TUL was more efficacious as compared to the combined treatment using FFC and LAOTC. As the first report, this field trial describes the successful treatment of undifferentiated respiratory diseases in large numbers of sheep. Thus, TUL can be used for the treatment of undifferentiated respiratory diseases in sheep. [J Adv Vet Anim Res 2015; 2(3.000): 279-284]

Duck plague (DP) is the most feared duck disease in the world. For isolation, identification, molecular detection and characterization of DP virus (DPV), a total of 94 samples were collected from commercial farms (n=6) and households (n=13) from Rajshahi (n=37), Netrokona (n=35) and Mymensingh (n=22) districts of Bangladesh. The samples were processed and inoculated into 11-13 days old embryonated duck eggs for virus propagation. Virus was identified using agar gel immunodiffusion test (AGIT) and passive hemagglutination (PHA) test, and was confirmed by polymerase chain reaction (PCR) targeting DNA polymerase and gC genes, followed by sequencing. Pathogenicity tests were performed using duck embryos, ducklings and ducks. Among the 94 samples, 17 isolates were confirmed as DPV by PCR amplification of partial DNA polymerase (446-bp) and gC genes (78-bp), respectively. One of the isolates (Anatid herpes 1 BAU DMH) was sequenced and found to be closely related with a Chinese variant of DPV (GenBank: JQ647509.1). Thus, we assume that both Bangladeshi and Chinese isolates of DPV may have a common ancestor.http://dx.doi.org/10.5455/javar.2015.b90

Duck plague (DP) is the most feared duck disease in the world. For isolation, identification, molecular detection and characterization of DP virus (DPV), a total of 94 samples were collected from commercial farms (n=6) and households (n=13) from Rajshahi (n=37), Netrokona (n=35) and Mymensingh (n=22) districts of Bangladesh. The samples were processed and inoculated into 11-13 days old embryonated duck eggs for virus propagation. Virus was identified using agar gel immunodiffusion test (AGIT) and passive hemagglutination (PHA) test, and was confirmed by polymerase chain reaction (PCR) targeting DNA polymerase and gC genes, followed by sequencing. Pathogenicity tests were performed using duck embryos, ducklings and ducks. Among the 94 samples, 17 isolates were confirmed as DPV by PCR amplification of partial DNA polymerase (446-bp) and gC genes (78-bp), respectively. One of the isolates (Anatid herpes 1 BAU DMH) was sequenced and found to be closely related with a Chinese variant of DPV (GenBank: JQ647509.1). Thus, we assume that both Bangladeshi and Chinese isolates of DPV may have a common ancestor. [J Adv Vet Anim Res 2015; 2(3.000): 296-303]

The objective of this study was to evaluate the effect of exogenous fibrolytic enzyme (EFE) and/or live yeast culture supplementation in total mixed ration (TMR) on rumen fermentation patterns in buffalo. For this, four adult buffalo bulls weighing 377.05±43.36 kg were randomly allotted to four dietary treatments viz., TMR containing R:C ratio of 70:30 (T1), T1 supplemented with EFE at 15 g/animal/day (T2), T1 supplemented with live yeast culture at 10 g/animal/day (T3), and T1 supplemented with EFEs at 15 g/animal/day and live yeast culture at 10 g/animal/day (T4). Rumen liquor from the fistulated animals was collected at 0, 1, 2, 4, 6 and 8 h post-feeding, and was analyzed. This study revealed that rumen pH values were highest at 0 h, and were declined to minimum by 4 h post-feeding, while total volatile fatty acids (TVFA), ammonia nitrogen (NH3-N), and nitrogen (N) fractions reached to peak at 4 h post-feeding, and later followed a decreasing trend in all the treatments. Supplementation of EFE in TMR (T2) had no effect (P>0.05) on rumen pH and food and protozoal N concentration, while it influenced to increase (P<0.01) the concentration of TVFA, NH3-N and other N fractions as compared to the T1. Yeast culture supplementation in TMR (T3) increased (P<0.01) rumen pH, TVFA, NH3-N, total N, TCA-insoluble N and residual N. However, no effect (P>0.05) on food and protozoal N in buffalo bulls was found. This study indicated that, supplementation of EFE and/or live yeast culture in TMR (T4) increased (P<0.01) the rumen pH, TVFA, NH3-N and N fractions in buffalo bulls as compared to the control group. Therefore, it is concluded that supplementation of EFE and/or live yeast culture in TMR can increase the concentration of rumen metabolites in buffalo bulls.  http://dx.doi.org/10.5455/javar.2015.b98

The objective of this study was to evaluate the effect of exogenous fibrolytic enzyme (EFE) and/or live yeast culture supplementation in total mixed ration (TMR) on rumen fermentation patterns in buffalo. For this, four adult buffalo bulls weighing 377.05+/-43.36 kg were randomly allotted to four dietary treatments viz., TMR containing R:C ratio of 70:30 (T1), T1 supplemented with EFE at 15 g/animal/day (T2), T1 supplemented with live yeast culture at 10 g/animal/day (T3), and T1 supplemented with EFEs at 15 g/animal/day and live yeast culture at 10 g/animal/day (T4). Rumen liquor from the fistulated animals was collected at 0, 1, 2, 4, 6 and 8 h post-feeding, and was analyzed. This study revealed that rumen pH values were highest at 0 h, and were declined to minimum by 4 h post-feeding, while total volatile fatty acids (TVFA), ammonia nitrogen (NH3-N), and nitrogen (N) fractions reached to peak at 4 h post-feeding, and later followed a decreasing trend in all the treatments. Supplementation of EFE in TMR (T2 and not;) had no effect (P>0.05) on rumen pH and food and protozoal N concentration, while it influenced to increase (P<0.01) the concentration of TVFA, NH3-N and other N fractions as compared to the T1. Yeast culture supplementation in TMR (T3) increased (P<0.01) rumen pH, TVFA, NH3-N, total N, TCA-insoluble N and residual N. However, no effect (P>0.05) on food and protozoal N in buffalo bulls was found. This study indicated that, supplementation of EFE and/or live yeast culture in TMR (T4) increased (P<0.01) the rumen pH, TVFA, NH3-N and N fractions in buffalo bulls as compared to the control group. Therefore, it is concluded that supplementation of EFE and/or live yeast culture in TMR can increase the concentration of rumen metabolites in buffalo bulls. [J Adv Vet Anim Res 2015; 2(3.000): 310-315]