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Investigation of helminths and protozoans infecting old world monkeys: captive vervet, cynomolgus, and rhesus monkeys
2010
Lee, J.I., Seoul National University Hospital, Seoul, Republic of Korea | Kang, S.J., Seoul National University Hospital, Seoul, Republic of Korea | Kim, N.A., Seoul National University Hospital, Seoul, Republic of Korea | Lee, C.W., Seoul National University Hospital, Seoul, Republic of Korea | Ahn, K.H., Seoul National University Hospital, Seoul, Republic of Korea | Kwon, H.S., Seoul National University Hospital, Seoul, Republic of Korea | Park, C.G., Seoul National University Hospital, Seoul, Republic of Korea | Kim, S.J., Seoul National University Hospital, Seoul, Republic of Korea
The objective of this study was to investigate the infection rate of gastro-intestinal tract parasites on acquired laboratory nonhuman primates, Vervet monkey, Cynomolgus monkey, and Rhesus monkey acquired from Japan and China. These monkeys have been acclimating at an individual housing condition after our legal quarantine period. We examined 133 fecal samples to investigate parasitic infection using direct smear and formalin-ether-sedimentation technique. As a result, total parasitic infection rate was 33.8% (n=45/133) for all monkeys. Two species of macaques, cynomolgus and rhesus, were infected with Trichuris trichiura (4), Giardia lamblia (4) and Balantidium coli (41). Vervet monkeys, which had been controlled by individual housing system for a long time, were clear for parasitic infection. The protozoan, Balantidium coli was one of the most frequently detected in these monkey colonies. Double infection was noted in only 4 monkeys and involved with Trichuris trichiura and Balantidium coli. Serious clinical symptoms were not observed in the most of the infected monkeys, but the monkeys infected by Giardia lamblia showed intermittent or chronic watery diarrhea. Consequently, the prophylactic anthelmintic treatment and periodic monitoring are essential to preserve the SPF colonies in the laboratory facility.
显示更多 [+] 显示较少 [-]Proteome analysis between diverse phenotypes of Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium)
2010
Shin, G.W., Chonbuk National University, Jeonju, Republic of Korea | Cha, I.S., Gyeongsang National University, Jinju, Republic of Korea | Lee, W.W., Busan Metropolitan City Institute of Health and Environmental, Busan, Republic of Korea | Nho, S.W., Gyeongsang National University, Jinju, Republic of Korea | Park, S.B., Gyeongsang National University, Jinju, Republic of Korea | Jang, H.B., Gyeongsang National University, Jinju, Republic of Korea | Kim, Y.H., Gyeongsang National University, Jinju, Republic of Korea | Jung, T.S., Gyeongsang National University, Jinju, Republic of Korea
Protein expression patterns in Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) strains with diverse phenotypes, such as phage type, antibiotic resistance pattern and plasmid profiles were examined. For detailed analysis of proteins expressed by different S. Typhimurium strains, protein fractions were divided into detergent-rich phase (DP) and aqueous phase (AP) using triton X-114 detergent. The two phases were subjected to two-dimensional gel electrophoresis (2-DE), followed by protein identification using peptide mass fingerprinting (PMF). In the results, PMF showed that DP fractions consisted mainly of outer membrane proteins, whereas the AP fractions included cytosolic proteins. Comparison of 2-DE profiles of DP did not show any distinct protein spots which could be correlated with phage type, antibiotic resistance pattern or plasmid profile. However, comparisons of 2-DE profiles of the AP revealed differences in the protein spots, which could be correlated with the plasmid profile and phage types. Among these protein spots, flagellin was specific for strains containing a 90 kb plasmid. Compared to DT193 phage type, three protein spots in the range of pI 5.0-5.5 and MW 8-15 kDa of AP 2-DE profiles were absent in the DT104 phage types. Additionally, a protein spot with PI in the range of 4.5-5.0 and molecular weight (MW) between 51-69 kDa was specific for phage type DT104, while a protein spot with pI in the range of 4.0-4.8 and MW between 18-20 kDa was specific for DT193 phage type. These protein spots may be useful for discriminating phage types of S. Typhimurium.
显示更多 [+] 显示较少 [-]Clinical characteristics of oral diseases on stray cats in Gwangju
2010
Myung, B.Y., Gwangju Animal Shelter, Gwangju, Republic of Korea | Kim, S.E., Chonnam National University, Gwangju, Republic of Korea | Shim, K.M., Nambu University, Gwangju, Republic of Korea | Ryu, J.W., Chosun University, Gwangju, Republic of Korea | Kim, S.H., Chonnam National University, Gwangju, Republic of Korea | Moon, C.J., Chonnam National University, Gwangju, Republic of Korea | Bae, C.S., Chonnam National University, Gwangju, Republic of Korea | Choi, S.H., Chungbuk National University, Cheongju, Republic of Korea | Kang, S.S., Chonnam National University, Gwangju, Republic of Korea | Park, H.J., Jeju National University, Jeju, Republic of Korea
Demand on specialized feline dentistry has increased over the years, partly due to growing feline population in Korea. Despite the notable increase in the population, not much researches has been conducted on oral disease for stray cats. Thus this research was conducted on 81 adult stray cats from Gwangju in Korea (42 males and 39 females) with average weight of 3.4 kg and also focused on occurrence of periodontal disease (plaque and calculus), feline odontoclastic resorptive lesion (FORL), stomatitis and tooth anomaly. Formation of dental plaque and calculus were abundant on maxilla than mandible, especially distributed much on 4th premolar teeth and 1st molar teeth. After thorough examination of oral cavity, 33 stray cats (40.7%) were found to have oral disease. Eighteen stray cats (22.2%) had tooth fracture which was evident on cuspid and missing teeth were present on 2nd premolar tooth of maxilla and 2nd incisor of mandible amongst 20 stray cats (24.7%). FORL was present on cuspids and 2nd premolar tooth of maxilla among 10 stray cats (12.3%). Stomatitis was involved with FORL, missing tooth, and tooth fracture. Therefore, it was determined that stomatitis, FORL, and periodontal disease were closely associated.
显示更多 [+] 显示较少 [-]Cystorrhexis with uroperitoneum during parturition in a Appaloosa neonatal foal
2010
Yang, J.H., Equine Hospital of Busan Race Park, Korea Racing Authority, Busan, Republic of Korea | Cheong, J.T., Jeju National University, Jeju, Republic of Korea | Lee, J.M., Jeju National University, Jeju, Republic of Korea | Hwang, K.K., Jeju National University, Jeju, Republic of Korea | Lim, Y.K., Jeju National University, Jeju, Republic of Korea
A 3-day-old Appaloosa colt was examined because of acute onset of ataxia, left-sided head tilt, fever and heart murmur. The foal continued to nurse but became increasingly depressed and mild colic. On plain radiological examination, the foal was normal. Uroperitoneum was diagnosed following abdominocentesis. Postmortem examination revealed bladder rupture. The lesion was in the dorsomedial part of the bladder wall. We suggested that the cause of bladder rupture was parturient trauma. Even though the rupture was associated with trauma, the margin of rupture of bladder was clearly smooth and regular.
显示更多 [+] 显示较少 [-]Treatment of prednisolone for eosinophilic enteritis in a dog
2010
Lee, J.W., Chungnam National University, Daejeon, Republic of Korea | Lee, S.H., Chungnam National University, Daejeon, Republic of Korea | Lim, S.J., Chungnam National University, Daejeon, Republic of Korea | Park, H.J., Chungnam National University, Daejeon, Republic of Korea | Jung, D.W., Chungnam National University, Daejeon, Republic of Korea | Kim, T.S., Chungnam National University, Daejeon, Republic of Korea | Kim, D.H., Chungnam National University, Daejeon, Republic of Korea | Song, K.H., Chungnam National University, Daejeon, Republic of Korea
A 7-year-old, castrated male Yorkshire terrier dog with chronic vomiting, and melena lasted for 3 weeks was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. The complete blood counts and serum biochemistry revealed no remarkable findings. Gastrointestinal series revealed irregular margin in small intestinal lumen. After upper gastrointestinal series, erythema of the small intestinal lesions was found by exploratory laparotomy and full-thickness biopsy was performed. Histopathologic examination revealed infiltration of predominantly eosinophils and other inflammatory cells in small intestine. Based on these findings, the dog was diagnosed as eosinophilic enteritis. After 4 weeks medical treatment and hypoallergenic diet, the patient showed no vomiting, melena and other complications.
显示更多 [+] 显示较少 [-]Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium
2010
Choresca Jr. Casiano H., Seoul National University, Seoul, Republic of Korea | Gomez, Dennis K., University of the Philippines in the Visayas, Miagao, Iloilo, Republic of the Philippines | Han, J.E., Seoul National University, Seoul, Republic of Korea | Shin, S.P., Seoul National University, Seoul, Republic of Korea | Kim, J.H., Seoul National University, Seoul, Republic of Korea | Jun, J.W., Seoul National University, Seoul, Republic of Korea | Park, S.C., Seoul National University, Seoul, Republic of Korea
Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.
显示更多 [+] 显示较少 [-]Serodiagnosis of porcine reproductive and respiratory syndrome virus infection with the use of glycoprotein 5 antigens 全文
2010
Glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) has been studied extensively as a target for vaccine development. This study evaluated the serodiagnostic application of PRRSV GP5 by enzyme-linked immunosorbent assay (ELISA). Two immunodominant peptides (VR #1 and VR #2) and two neutralizing ectodomain-containing peptides (Ecto #1 and Ecto #2), as well as recombinant GP5 (rGP5) as a control, were prepared. Serum from unvaccinated pigs was screened for the antibodies that bind to these peptide and protein antigens. The results were compared with those from a commercially available diagnostic ELISA kit (HerdChek), which uses the nucleocapsid (N) protein as an antigen. Only VR #1+#2 showed a result statistically similar to that of N protein. Ecto #1 and Ecto #2 had a lower sensitivity than VR #1+#2 and rGP5. The peptides and rGP5 showed significant associations with the N protein (P < 0.05 or 0.01), which suggests that GP5 may also be a candidate serodiagnostic antigen. Since antibodies against GP5 persist much longer than those against the N protein, GP5 itself and some of its fragments are thought to be good targets for serodiagnosis. In addition, the presence of antibodies against the PRRSV structural antigens showed significant antigen-dependent differences.
显示更多 [+] 显示较少 [-]Sensory nerve conduction and nociception in the equine lower forelimb during perineural bupivacaine infusion along the palmar nerves 全文
2010
The purpose of this investigation was to study lateral palmar nerve (LPN) and medial palmar nerve (MPN) morphology and determine nociception and sensory nerve conduction velocity (SNCV) following placement of continuous peripheral nerve block (CPNB) catheters along LPN and MPN with subsequent bupivacaine (BUP) infusion. Myelinated nerve fiber distribution in LPN and MPN was examined after harvesting nerve specimens in 3 anesthetized horses and processing them for morphometric analysis. In 5 sedated horses, CPNB catheters were placed along each PN in both forelimbs. Horses then received in one forelimb 3 mL 0.125% BUP containing epinephrine 1:200 000 and 0.04% NaHCO3 per catheter site followed by 2 mL/h infusion over a 6-day period, while in the other forelimb equal amounts of saline (SAL) solution were administered. The hoof withdrawal response (HWR) threshold during pressure loading of the area above the dorsal coronary band was determined daily in both forelimbs. On day 6 SNCV was measured under general anesthesia of horses in each limb’s LPN and MPN to detect nerve injury, followed by CPNB catheter removal. The SNCV was also recorded in 2 anesthetized non-instrumented horses (sham controls). In both LPN and MPN myelinated fiber distributions were bimodal. The fraction of large fibers (>7 μm) was greater in the MPN than LPN (P < 0.05). Presence of CPNB catheters and SAL administration did neither affect measured HWR thresholds nor SNCVs, whereas BUP infusion suppressed HWRs. In conclusion, CPNB with 0.125% BUP provides pronounced analgesia by inhibiting sensory nerve conduction in the distal equine forelimb.
显示更多 [+] 显示较少 [-]BACTERIOLOGICAL, CYTOLOGICAL AND BIOCHEMICAL 全文
2010
Ashwaq Rahem Nazaal
ABSTRACT Bacteriological, cytological and biochemical testing were performed on synovial fluid of 100 (50 female and 50 male) slaughtered cows seen in Basrah slaughter house. The bacterial isolation revealed that the incidence of infected caw was 17(34%) in female and 11(22%) in male. Bacterial isolates include Staphylococcus aureus and Corynobacterium pyogenes. The results of biochemical testing indicate that mucin clot test varied from poor to fair clotting, the total protein of infected animals significantly higher than that of control group. While the cytological analysis showed that the total leuckocytic count was significantly higher (P
显示更多 [+] 显示较少 [-]SYNTHESIS, STUDIES THEIR QUANTUM MECHANICAL PROPERTIES AND BIOLOGICAL ACTIVITY OF NEWER ANTIPYRIN DERIVATIVE AND ITS (CU2+) COMPLEX 全文
2010
Tememe | Al - Hrashawy | R. M | E. H. Al
The synthesis of (C16H14N3O2) ligand and (Cu2C32H28N6O12S2) complex are prepared in this work . The newly prepared compounds were characterized by IR spectroscopy and elemental analysis . The determination of the antimicrobial activity of ligand and its complex was carried out on sample of Escherichia coli , Klebsiella pneumonia , Staphylococcus aureus and Bacillus cereus . The quantitative antimicrobial activity test results proved that all the prepared compounds are very active against Bacillus cereus and that complex very active against Escherichia coli.. Quantum mechanical calculation confirmed the assumption of stability of prepared compounds which agreement with antimicrobial activity results .
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