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Endometrial prostaglandin F2α in vitro production and its modulation regarding dominant follicle position in cattle
2018
Fabiana Fernandes Bressan | Claudia Maria Bertan Membrive | Marcelo Demarchi Goissis | Vanessa Belentani Marques | Pauline Martins da Cunha | Mario Binelli
Prostaglandin F2α (PGF2α) determines luteolysis in cattle, and the ability to manipulate its endogenous synthesis is indispensible for large-scale animal breeding. Estradiol (E2) and progesterone (P4) modulate several molecular pathways in endometrial cells, including the synthesis of PGF2α; however, its specific mechanisms are still not totally known. This study investigated the production in vitro and possible modulation of endometrial PGF2α due to a local effect of endogenous E2 in the ipsilateral uterine horn (UH) containing the dominant follicle (DF) or from P4 in ipsilateral horn containing the corpus luteum (CL). The PGF2α stimulators oxytocin (OT) and phorbol 12,13-dibutyrate (PDBu) were incubated with endometrial explants, and PGF2α content was measured. For that, cycling cows were synchronized, the development of DF and CL was examined by ultrasonography and on the seventh day of the estrous cycle, endometrial explants were collected and cultured in medium supplemented with 10-6 M PDBu or 10-6 M OT or non-supplemented. Media samples were collected immediately after treatment and 60 min later. Radioimmunoassay showed that the PGF2α content of the UH ipsilateral to the DF was 49% less than that of the contralateral UH (8.22 ± 0.95 vs. 12.24 ± 0.95 pg/mL/mg tissue, respectively; P < 0.01). However, the PGF2α levels did not differ between the UHs as a function of the CL position (9.46 ± 0.95 vs. 11 ± 0.95 pg/mL/mg; P > 0.05). The cellular stimulators promoted an increase in PGF2α synthesis (P < 0.02), and the effects differed among the animals (P < 0.04). The PGF2a production was higher in the explants treated with PDBu rather than OT (13.68 ± 1.16 vs. 10.01 ± 1.16 pg/mL/mg tissue, respectively; P < 0.05). In conclusion, PGF2α synthesis is modulated by the presence of the DF (local E2) but not the CL (local P4), and both PDBu and OT stimulated PGF2a synthesis.
显示更多 [+] 显示较少 [-]Detection and Molecular Characterization of Parvovirus Serotypes in Egypt
2018
Amira A. T. A. AL-Hosary
Parvovirus infection is one of the most common enteric diseases affecting dogs in Egypt. A total number of 49 dogs younger than six month old, suffering from severe watery to bloody diarrhea, vomiting and lethargy were examined to confirm the infection with parvovirus through detection of the viral antigen serologically by FASTest® PARVO Card Test and genetically by using specific primers for both conventional and nested polymerase chain reaction (PCR). Serological assay confirmed the infection in 81.63% (40/49) of the examined animals, while the molecular techniques confirmed the infection in 81.63% (40/49) by the conventional PCR and in 97.96% (48/49) by the nested PCR. Sequencing revealed that CPV2a and CPV2b serotypes are circulating in the Egyptian field. The obtained sequences were submitted to the GenBank under the following accession MG764314, MG764315, MG930487 and MG930488. Serotype CPV2b showed some mutation in different sites.
显示更多 [+] 显示较少 [-]Studies on the Effects of Enrofloxacin Overdose on Different Health Parameters in Broiler Chickens
2018
H.F. Ellakany | I.M. Abu El-Azm | A.A. Bekhit | M.M. Shehawy
The effect of 10 times (10x) overdose of enrofloxacin was studied in broiler chickens. One hundred and eighty chicks were classified in 3 equal groups. The first group received normal theurapeutic dose of enrofloxacin (1x) in drinking water for the first 5 consecutive days of age and repeated again at 24th -28th day of age. The second group received 10x (overdose) at the same ages. The third group was left non-medicated as a control group. Blood samples were taken on the 6th, 14th, 29th and 34th day of age for different laboratory tests. Enrofloxacin at 10x caused a decrease in the value of the following parameters: HI antibody titers to NDV vaccine at the 14th and the 34th day of age, serum albumin at the 10th day of age, hemoglobin at the 29th and the 34th day, lymphocytic count and IBDV ELISA titers at 29th day of age, uric acid at 29th day, phagocytic activity at 34th day, Lactobacillus spp. count in duodenum, feed conversion efficiency and body weight gain. The 10x (overdose) increased serum urea and creatinine at 29th day of age, serum AST and ALT at 29th and 34th day of age, and heterophilic count. Histopathological degeneration in liver, spleen, kidneys, bursa of Fabricius and thymus were demonstrated by 10x (overdose) of enrofloxacin. Challenge with vNDV caused 66.6% mortality in birds received the 10x (overdose) compared with 33.3% in the vaccinated non treated control group.
显示更多 [+] 显示较少 [-]Nosocomial Antiseptics And Disinfectants Resistant Bacteria: microbiological and histopathological studies
2018
Maged S. Ahmad | Medhat Abdel Fatath | Khalid A. El-Nesr | Mohamed Ramadan
This study contains evidence-based recommendations for the prevention of hospital acquired infections. Hospital acquired infections are a major cause of mortality and morbidity and provide challenge to clinicians. Measures of infection control included identifying patients at risk of nosocomial infections. Antiseptics and disinfectants are used extensively in hospitals and other health care settings for a variety of topical and hard-surface applications. In particular, they are an essential part of infection control practices and aid in the prevention of nosocomial infection. This study investigated the nosocomial infection via isolation and identification of bacterial pathogens, the effect of detergents against isolated bacteria, and describes the histopathological tissue changes induced by the isolated bacteria in vivo study. Some bacteria resistant to disinfectants were prevalent and these bacteria had different effects on the human, Different pathological changes were seen experimentally by inoculation of these bacteria in mice.
显示更多 [+] 显示较少 [-]Epidemiological Studies on Transmission of some Avian Pathogens from Fish Farms to Water Fowls in Kafr El-sheikh Governorate, Egypt
2018
Anwaar M. El-Nabarawy | Mohamed A. Shakal | Abdel-haleem M. Hegazy | Mohamed Mohamed Batikh
Integrated Waterfowl is common in Kafr El-Sheikh Governorate. The infection with many bacterial and /or viral diseases due to using poultry litter as a fertilizer in fish farms is studied. Fifty litter samples, sixty nine fish pond water samples, two hundred fecal swabs from integrated waterfowl and sixty samples from liver from these waterfowl were collected for surveying some pathogens which may be present in litter and transported to fish ponds and infect waterfowl. Results of this survey revealed the isolation of 19 Salmonella spp. (4 isolates from litter, 2 isolates from fish pond water, 8 isolates from fecal swabs of waterfowl and 5 isolates from waterfowl liver), 17 Staphylococcus spp. (7 isolates from litter, 3 isolates from fish pond water, 5 isolates from fecal swabs of waterfowl and 2 isolates from waterfowl liver) and 59 E. coli isolates (9 isolates from litter,16 isolates from fish pond water, 22 isolates from fecal swabs of waterfowl and 12 isolates from waterfowl liver) isolates were obtained from different samples.
显示更多 [+] 显示较少 [-]Antioxidant, immunostimulant and renal protective activities of tri-herbal combination in African Sharptooth Catfish, Clarias gariepinus
2018
Walaa F.A. Emeish | Zeinab Al-Amgad | Hassan Ahmed
Medicinal herbal feed that used as feed additives are widely applied in livestockproduction and may be applicable to aquaculture production systems. Thepresent study explores the modulatory effects of incorporation of herbalcombination of black cumin seeds (Nigella sativa), peppermint (Menthapiperita) and fennel (Foeniculum vulgare), on African Sharptooth Catfish,Clarias gariepinus. For this purpose, a total of 72 catfish were randomlydistributed into 6 groups of 12 fish each and fed on the experimental diets for 45successive days. Fish of first group were fed on basic diet only while, those ofthe second one were fed on basic diet supplemented with 0.5% from eachmedicinal plant while, other 4 groups were subjected to challenge experiment byinjection with Vibrio vulnificus. The third group was kept non-injected, fourthgroup was injected by saline, fifth and sixth groups were injected by Vibriovulnificus after feeding with basic diet and basic diet with herbs, respectively.Results proved that, total antioxidant capacity, serum total proteins, albuminsand globulins were observed to be significantly higher in the treated group ascompared to the control. Lymphocytes percent increased significantly in herbalfed group comparing to the control group while, neutrophils percent decreasedsignificantly. Although serum urea level was not affected by herbal supplement,serum creatinine level was decreased significantly. Body weight gain of C.gariepinus increased significantly after herbal administration. Catfish challengedwith a Vibrio vulnificus isolate and received the herbal diet showed lessmortality than the control group. Fish fed on the herbal diet exhibited normalhistological structure of liver, kidney and spleen. In conclusion, based on thecurrent results together with the low cost and the potential antioxidant andimmune effects of mixed herbal medicinal plants used in the study, it isrecommended to be used in fish feed to diminish the mortalities caused by someaquatic pathogens.
显示更多 [+] 显示较少 [-]The efficacy of diclazuril (liquid formulation) in the prevention and control of coccidiosis in broiler chicken.
2018
M. M. Amer | Wafaa A. Abd el-Ghany | Aziza M. Amer | K. M. Ali | A. E. A. Hanafei | G. A. Zohair
This study was carried out to evaluate the efficacy of water soluble formulation of diclazuril (1%) in the prevention and control of mixed Eimeria infection in two experimental (semifield) trials and testing efficacy of this medicament in controlling of natural field case of mixed Eimeria infection. The experimental (semifield) trials carried out each on 150-day-old broiler chicks, as they were divided into 3 equal separate subgroups. Infected groups were orally received 1ml of an inoculum containing 50.000 sporulated oocysts of Eimeria tenella, E. acervulina and E. necatrix/ chick at 14 days of age. Diclazuril (1%) liquid at dose of 1 ml / 4 liter of drinking water for 2 successive days just after appearance of bloody dropping (at the 5th day post infection). Birds were given prophylactic diclazuril dose (1 ml / 8 liter) at the 7th day before the experimental inoculation. In both trials were oocyst count (shedding) in the dropping, dropping score, lesion score, body weight gain % and mortality rate were evaluated. Groups treated with diclazuril either in the prophylactic and curative studies gave significant and satisfactory improvement in the assessment criteria when compared with infected non medicated groups. A field trial was conducted 2 commercial broiler chicken farm consisted of 2 flocks aged 33 days; 6000 birds each. Flock (1) was kept as control positive infected non medicated group, whereas flock (2) was medicated with diclazuril (1%) at dose of 1 ml / 4 liter of the drinking water for 2 successive days. The results revealed more reduction in total oocyst count, lesion score and the mortality rate in the medicated than the infected non-treated group. The results proved that diclazuril (1%) liquid is effective in prevention and control of both experimental and field infection field circulating Eimeria species still sensitive to the drug.
显示更多 [+] 显示较少 [-]Neonatal lamb behaviour and thermoregulation with special reference to thyroid hormones and phosphorous element: Effect of birth weight and litter size
2018
R. A. Darwish | S. M. El-Bahr
New-born lambs have limited energy reserves and need a rapid access to colostrum to maintain homeothermy and survive. The object of this study was to investigate the importance of neonatal lamb behaviour in the maintenance of its body temperature and survival. The time taken to stand and suck after birth and rectal temperatures were determined in 72 crossbred lambs (progeny of crossing 1/2 Rhamani and 1/2 Finnish). In addition, blood samples were collected at 1, 24 and 72 h after birth. The obtained sera were assayed for thyroid hormones (T3 and T4), known to be involved in heat production and phosphorous element (ph) which is essential for energy metabolism. The obtained results revealed that, low birth weight and triplet lambs were behaviourally less active at birth and had less T3, T4, Ph and body temperatures values over the first 3 days of life than lambs of heavy or medium birth weight and single or twin lambs. Thus, light weight and triplet lambs were highly susceptible to hypothermia and were exposed to high rate of mortality (18.75 and 16.66%) during the neonatal period than other lambs (3.84, 10.00, 4.55, and 9.38 %, respectively). Therefore, the present study concludes that neonatal lamb behaviour, specifically success in standing and obtaining colostrum are extremely important for thermoregulation and survival of the neonate.
显示更多 [+] 显示较少 [-]Determination of the optimal inactivation time of FMD virus Type "A" using BEI inactivator during the outbreak 2006 in Egypt and its effect on the antigenicity of the virus
2018
A. A. Omar | H. M. A. Azab | M. M. Abdel-Aty | S. M. Ali | M. A. Farag
FMD virus type A/1/ Egypt 2006 was inactivated with 0.1 M of BEI (Binary ethylene imine) formed by cyclization of 2- Bromoethyl-amine hydrobromide (BEA) in 0.2 N NaoH at 37oC with PH 8.0 for 24 hours. The virus was complete inactivated after 15 hours post inactivation. No residual virus particles were detected when inoculated in tissue culture. The inactivation rates are linear with a regular loss of titer ranged from 0.5- 1.0 log10 / hour. Control sample of virus at 37oC without BEI showed only a loss of 1.0 log from the original infectivity titer after 24 hours. The sample of virus which kept at -20oC, without BEI, showed loss 0.3 log10 from its original infectivity titer after 24 hours. There is no change in the complement fixing antigen before and after inactivation process with BEI inactivator and in the CFT 7 dilution of antigen was stable (fixed) pre and post inactivation of virus. Also it was found that the inactivation rate of BEI was higher than the inactivation with pure Ethylenimine (EI) and formalin.
显示更多 [+] 显示较少 [-]Isolation and identification of bovine herpes virus -1 (BHV-1) from semen of foreign breeds bulls
2018
H. M. Madbouly | S. M. Tamam | A. M. Abd-El-Gaid
Bovine herpes virus-1 (BHV-1) was isolated from bull semen by inoculation onto chorioallantoic membrane of specific pathogen free eggs. The isolated virus was identified by agar gel precipitation test, Dot ELISA, pock reduction and neutralization test, as well as by histopathology. The isolated virus was propagated on Madin Darby Bovine Kidney cells and identified by polymerase chain reaction. In sero-survey for BHV-1 antibodies on 1091 collected serum samples 188 sera showed clear precipitation lines by AGPT
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