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Comparison of microscopy, card agglutination test for Trypanosoma evansi, and real-time PCR in the diagnosis of trypanosomosis in dromedary camels of the Abu Dhabi Emirate, UAE 全文
2022
Habeeba, Shameem | Khan, Rashid Ali | Zackaria, Hassan | Yammahi, Saeed | Mohamed, Zulaikha | Sobhi, Wissam | AbdelKader, Ayman | Alhosani, Mohamed Ali | Muhairi, Salama Al
Comparison of microscopy, card agglutination test for Trypanosoma evansi, and real-time PCR in the diagnosis of trypanosomosis in dromedary camels of the Abu Dhabi Emirate, UAE 全文
2022
Habeeba, Shameem | Khan, Rashid Ali | Zackaria, Hassan | Yammahi, Saeed | Mohamed, Zulaikha | Sobhi, Wissam | AbdelKader, Ayman | Alhosani, Mohamed Ali | Muhairi, Salama Al
Trypanosomosis is an important disease of dromedary camels caused by the pathogenic protozoan Trypanosoma evansi. This study aimed to compare three different tests for its diagnosis in this species: conventional microscopy, the card agglutination test for trypanosomosis/T. evansi (CATT/T. evansi) and real-time PCR. Whole blood and serum samples collected from 77 dromedary camels of Abu Dhabi, United Arab Emirates, were analysed with the test methods stated. Statistical analysis was done using McNemar’s chi-squared test, and Cohen’s kappa index (κ) was calculated. We obtained results with positivity of 18% (14/77) by microscopy, 22% by CATT (17/77) and 60% (46/77) by real-time PCR, with the chain reaction detecting at a respectively three- and two-fold greater rate than the other techniques. Analysis of the data revealed a relative sensitivity of 30.4% and 37.0% for microscopy and CATT, respectively, compared to real-time PCR. The difference between the real-time PCR’s sensitivity and those of the other methods was statistically significant, with X² values of 30.03 and 20.1, respectively (df = 1 and P = 0.05 in both cases). Agreement of microscopy results with those of with CATT was good (κ = 0.72; 95% CI = 0.62–0.82). Cohen’s kappa index showed fair agreement of real-time PCR with microscopy (κ = 0.26; 95% CI = 0.16–0.36) whereas it was in poor agreement with CATT (κ = 0.09; 95% CI = 0.02–0.15). Real-time PCR was found to be more sensitive than microscopy and CATT.
显示更多 [+] 显示较少 [-]Comparison of microscopy, card agglutination test for Trypanosoma evansi, and real-time PCR in the diagnosis of trypanosomosis in dromedary camels of the Abu Dhabi Emirate, UAE 全文
2022
Habeeba Shameem | Khan Rashid Ali | Zackaria Hassan | Yammahi Saeed | Mohamed Zulaikha | Sobhi Wissam | AbdelKader Ayman | Alhosani Mohamed Ali | Muhairi Salama Al
Trypanosomosis is an important disease of dromedary camels caused by the pathogenic protozoan Trypanosoma evansi. This study aimed to compare three different tests for its diagnosis in this species: conventional microscopy, the card agglutination test for trypanosomosis/T. evansi (CATT/T. evansi) and real-time PCR.
显示更多 [+] 显示较少 [-]Dirofilaria repens in dogs and humans in Slovenia 全文
2022
Kotnik, Tina | Rataj, Aleksandra Vergles | Šoba, Barbara
Dirofilaria repens in dogs and humans in Slovenia 全文
2022
Kotnik, Tina | Rataj, Aleksandra Vergles | Šoba, Barbara
The prevalence of Dirofilaria repens in dogs in countries bordering Slovenia ranges from 1.5% to 47.3%. The aim of this study was to estimate its prevalence in Slovenian dogs and to present the cases of dirofilariasis diagnosed in humans from 2010 to 2020. Epidemiological data were collected and blood samples were taken from 465 dogs older than one year and born in Slovenia. A real-time PCR was performed on all samples to detect filarioid DNA, and a D. repens-and D. immitis-specific real-time PCR was performed on positive samples. Blood samples from 446 dogs were tested for Dirofilaria spp. using a modified Knott’s test. Human cases were diagnosed from histological sections of excised subcutaneous nodules. Descriptive statistics were used to characterise the samples. The one-sample nonparametric chi-squared test was used to assess whether categories of a variable were equally distributed. Three dogs’ samples tested positive for D. repens using the species-specific real-time PCR, while D. immitis DNA was not detected. The modified Knott’s test was positive in two of the three PCR-positive dogs, two of which had never travelled outside Slovenia’s borders. Four human patients with D. repens dirofilariasis were diagnosed. Since their travel history was unknown, autochthonous transmission could not be confirmed. Our study demonstrated a 0.64% prevalence of D. repens infection in dogs in Slovenia. Two cases could be autochthonous.
显示更多 [+] 显示较少 [-]Dirofilaria repens in dogs and humans in Slovenia 全文
2022
Kotnik Tina | Rataj Aleksandra Vergles | Šoba Barbara
The prevalence of Dirofilaria repens in dogs in countries bordering Slovenia ranges from 1.5% to 47.3%. The aim of this study was to estimate its prevalence in Slovenian dogs and to present the cases of dirofilariasis diagnosed in humans from 2010 to 2020.
显示更多 [+] 显示较少 [-]Comparison of Vaginal Flora, Vaginal Cytology, Blood Values and Hormone Level of Cats in Different Reproductive Periods 全文
2022
Termelioğlu, Levent | Kalender, Hakan | Erat, Serkan
The aim of this study was to investigate vaginal cytology, haematological and hormonal values, the presence of bacteria in the vagina, and the relationship between these findings in different reproductive periods in cats. The study consisted of 30 healthy non-geriatric female cats that had reachedto puberty. The cats were divided into 3 equal groups (each having 10 cats) as estrus, anestrus and pregnant. The vaginal samples for microbiological and cytological examination andthe blood samples for hormonal analysis and hemogram were taken at the same time. A total of 100 vaginal epithelial cells were counted from the random areas of thevaginal cytology samples on the slide. The distributions of the percentages of the counted cells according to the groups were subtracted and compared. While there was no bacterial growth in 9 (30%) animals, bacterial growth was observed in 21 (70%) animals. There were no bacterial growth in 3 (30%), 4 (40%) and 2 (20%) animals inestrus, pregnant and anestrus groups, respectively. Estradiol (E2) level (42.64 ± 10.62 pg/ml) in estrus animals was significantly higher (P<0.001) than E2 level in pregnant and an estrus animals. The progesterone (P4) level of the pregnant group (12.22±9.35 ng/ml) was higher (P<0.001) than the P4 levels of the anestrus (0.84±0.25 ng/ml) and the estrus group (0.58±0.28 ng/ml), while the P4 levels of the estrus and the anestrus groups were similar. Significant differences were detected only in MCV, MCH and MCHC, within 19 blood parameters. MCV values were found to be lower in estrus animals (45.68±3.75femtoliter) than only in pregnant (51.21±4.99femtoliter) animals (P=0.007). The difference in MCH values between the estrus group (14.37±0.84 pg) and the pregnant group (15.62 ± 1.18 pg) (P=0.003) and the difference in MCHC values between the pregnant group (30.66±1.17 g/dl) and the anestrus group (32.42±1.04 g/dl) (P<0.001) were statistically significant. The presented results may help in the planning of future studies and the comparison of the obtained values.
显示更多 [+] 显示较少 [-]Questionnaire Study on Feeding Practices of Pace Horses in Aegean and Marmara Regions of Turkey 全文
2022
Danyer, Erdem | Bilal, Tanay
This sectional study aims to collect data about feeding practices for pace horses in the Aegean and Marmara regions of Turkey. For this purpose, a questionnaire consisting of 69 free text and multiple-choice questions was developed. The questionnaire was implemented on 29-31 August 2014 by personal interviews and was online on social media for ten months. The questionnaire comprised sections on breeder demographic information, farm condition, feeding habit, and nutritional disease. Fifty volunteer horse owners who had a total of 271 horses have participated in the study. Evaluation of the results focused on nutrition practices. Unfortunately, 46% of the respondents were not rasping their horses’ teeth, and an important part of them was not using supplementary oil (78%) or feed additives (84%). Drinking water after exercise (74%) as well as rotten, mouldy, and bacterially contaminated feed (72%) were reported as the most important colic reason by the responders. Based on the results of our study, educational initiatives are needed to inform pace horse owners about appropriate nutrition and related diseases to increase the pace horse race performance.
显示更多 [+] 显示较少 [-]Social Network Analysis of Giardiasis Positive Calves to those of Healthy One 全文
2022
Alıç Ural, Deniz | Ural, Kerem | Erdogan, Songul | Ayan, Adnan | Erdoğan, Hasan
In the present multidisciplinary study, the researchers on a field trial performed a Social Network approach and a group of 9 calves (3 males/6 females) aged 10 to 16 weeks. A 24 hours digital camera was used for location registration (X-Y position) and interactions on each calf, both individually and collectively. Based on the simple methodology nearest neighbor matrix positive and negative associations were analyzed. The field study lasted 4 weeks and the maximum interaction day was chosen, when individual calf activity was high. To those of 9 calves solely no 2 was infected with giardiasis on the first week, whereas calves with no:2, 7, and 8 were also infected on the second week. No: 4 and 6 were also infected with a total of 5 calves on the third week. This was followed by the final 6 calves in total infected. During the observation period, both by investigators and camera records, infected calf 2 was in close contact with no: 8 (at morning observation) and a slight degree of contact with no: 3 and 4 which were detected with giardiasis on the third and fourt weeks, respectively. Due to close contact with no: 8, infection was determined on the second week, earlier than others (except no: 7). Calves with no: 1 and 5 were never infected nor determined by PCR, fecal smear, or rapid diagnostic test kits throughout the study. In conclusion, the present authors suggested that social networking might be an important predisposing factor for giardiasis infection among calves.
显示更多 [+] 显示较少 [-]Investigation of Physical Properties and Mineral Contents of Drinking Water in Some Villages of Van and Mardin Provinces 全文
2022
Baraj, Merve Gizem | Mert, Nihat | Mert, Handan | Günbatar, Nizamettin
This study was carried out in order to examine a total of 40 drinking water samples taken from 20 locations selected from Mardin and Van provinces in terms of physical properties, heavy metal and mineral contents and to evaluate the quality of drinking water in accordance with the results obtained. Among the ion and mineral analyses of the samples, arsenic, copper, boron, cadmium, cobalt, manganese, nickel, lead, selenium and zinc analyses were made with Inductively Coupled Plasma and Mass Spectrometer, and magnesium analyses were made with Inductively Coupled Plasma-Optical Emission Spectrometer. Fluoride, chloride, nitrite, nitrate, bromide, sulfate and phosphate analyses were also performed by Dionex ion chromatography. Conductivity, pH, ORP, TDS and minimum salt analyses were performed with the Myron L device. As a result of the study, the difference between the fluoride, chloride, nitrite, nitrate, bromide, boron, cadmium, cobalt, zinc, conductivity, TDS, ORP and temperature values of the water samples of the two provinces was found to be significant. In addition, drinking water containing heavy metals was found to be above the limit values set by the Regulation on Water Intended for Human Consumption, nickel was detected in water samples taken from Van, and phosphate was not detected in samples taken from Mardin. The remarkable issues in water samples taken from 40 different locations in two provinces are presented in articles and it has been emphasized that it is important for the people living in this region to present the detected defects to local governments, environmental and public health units and to eliminate the defects.
显示更多 [+] 显示较少 [-]A New Era Under Soil Based Probiotics for Anti-Pruritic Combat Among Cats with Feline Atopic Skin Syndrome 全文
2022
Ural, Kerem | Erdogan, Hasan | Erdogan, Songul
Present research was conducted at the University of Aydin Adnan Menderes, Faculty of Veterinary. In a total of 10 cats with pruritic dermatoses were allocated, classified [Feline atopic skin syndrome (FaSs)] and adapted to the retrospective case controlled study on the basis of Polycheck in vitro Allergy Test, clinical signs, dermatoscopic and cytological examination. No prior drug prescription, nor immunosuppressive treatment were evident, which was an inclusion criteria. Complete blood count, serum biochemistry, and endocrine panels were all deemed available on days 0 and 21 for all cats involved. The Feline Dermatitis Extent and Severity Index (FeDESI) and a 10 cm visual analog scale (VAS) were deemed available as relevant prognostic parameters. A three strain invoving soil-based probiotic used via rectal route significantly decreased FEDESI and VAS pruritus scores after 10 days of rectal use. Pre-treament day 0 FEDESI scores (median ± SD) (106.3 ± 41.38), were significantly higher than scores on day 10 (15.3 ± 10.53) switching the severity of the disease to mild status in all cases. Furthermore, day 0 VAS pruritus scores were 7.4 ± 1.78 (mean ± SD) (prior to treatment), whereas owner VAS pruritus score was decreased to 1.3 ± 1.06 (mean ± SD) significantly (p=0.005). It should not be unwise to draw preliminary conclusion that 3 chained soil-based Bacillus probiotic was capable of short term cure for FaSs. Bacillus probiotics with safety usage should be added to therapeutical armamentarium of FaSs.
显示更多 [+] 显示较少 [-]Investigation of Lipid Mobilization and Oxidative Stress Parameters in The Serum Before and After Surgery of Cows with Left Displacement Abomasum 全文
2022
Aslan, Nurcanan | Yiğitarslan, Kürşat | Büyükoğlu, Tülay
Left Abomasum Displacement (LDA) is one of the most important metabolic diseases caused by negative energy balance during the early lactation period for high milk efficient cows. This study aimed to investigate the lipid mobilization and oxidative stress parameters in cows with LDA before and after the operation. In this research, cows with LDA (n=16) were divided into three groups that are before operation (pre-op LDA), immediately after the operation (post-op LDA), and on the 10th day after the operation (post-op 10 LDA). Control groups were formed from early lactation cows (n=8) and dry period (n=8). In serum samples collected from the study groups, total cholesterol, HDL and LDL cholesterol, triacylglycerol, free fatty acid (FFA), β-hydroxybutyric acid (BHBA), malondialdehyde (MDA) levels, and AST, GGT activities were determined spectrophotometrically, total antioxidant level (TAS), total oxidant level (TOS) and paraoxonase 1/arylesterase (PON1/ARES)] enzyme activity was measured according to the procedure of the colorimetric kit. Serum TOS and MDA levels increased in cattle with pre-op LDA compared to control groups, and MDA levels decreased to normal levels in both groups after the operation. TAS levels and PON1/ARES activities decreased in cattle with pre-op LDA compared to control groups and increased gradually in post-op groups. Serum total cholesterol, HDL, and LDL cholesterol levels decreased in cattle with pre-op LDA compared to the control groups and did not return to normal levels in the groups with post-op LDA. While BHBA levels and AST activities increased in cattle with pre-op LDA compared to control groups, they reached normal values in cattle with LDA on the post-op 10th day. It has been concluded that in evaluating the diagnosis, treatment, and prognosis of the disease in dairy cows with LDA, oxidative stress parameters such as TAS, TOS, and PON1/ARES may be used together with lipid parameters.
显示更多 [+] 显示较少 [-]The Light and Scanning Electron Microscopic Structure of Pecten oculi in the Goose (Anser anser) 全文
2022
Korkmaz, Deniz | Harem, Ismail Sah
This study aims to establish the light and electron microscopic structure of the pecten oculi in the goose (Anser anser). For this purpose, 12 samples of pecten oculi extracted from 6 goose eyes were used. In the study, it was found that the goose pecten consists of 13-14 pleats. The maximum transversal length of the eye was approximately 10 mm, the corneal diameter was 5 mm, the basal length of the pecten was 7 mm, the apical length was 1.5 mm, and the height of the pecten was 5.55 mm (n=6). In pecten pleats, the mean diameters of two separate vessels, primary and secondary, were 48.94 and 23.36 μm respectively. The primary vessels located at the centre of the pecten pleats were surrounded by the secondary vessels. It was observed that the melanocytes in pleats gradually intensified from basal to apical regions. Pecten covered to the vitreo-pecteneal limiting membrane and, hyalocytes were found on this part. This study revealed that the goose pecten has a structure similar to the avian species in the waterfowl family.
显示更多 [+] 显示较少 [-]Zonulin as a Noninvasive Selected Biomarker of Gut Barrier Function Identify and Debug Calves Suffering from Diarrhea 全文
2022
Alıç Ural, Deniz
Zonulin elucidated as a thoroughly known protein, is capable of modulating the gut integrity of intercellular connections. Intestinal permeability and its modulation by zonulin have been well-defined. Zonulin levels could increase in response to several stimuli, i.e. infection/gluten ingestion. Even if the latter occurs, zonulin signals into the body for elevating the permeability of the gut lining, permitting larger molecules to pass through. All aforementioned conditions initiate inflammation. In the present prospective field study, the aim was to determine the specificity of zonulin as a noninvasive selected biomarker of gut barrier function to identify and debug calves suffering from diarrhea. Furthermore, another purpose was to define the appearance of leaky gut (LeaG) among calves with diarrhea. By use of commercially available Bovine Zonulin ELISA test kits with a well-designed methodology all 11 diseased and other relevant healthy calves gave positive test results. Circulating zonulin levels (ng/mL) expressed as (±SEM), there were significant differences (p<0.001) between healthy (26.43±3.528) and diarrheic calves (57.97±4.250). As a preliminary conclusion, it should not be unwise to draw the hypothesis that zonulin levels debug diarrheic calves from healthy ones. Further studies are warranted.
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