The present study has been carried out to investigate the effect of three neurotransmitters (Glutamate, L-Arginine and GABA) on some aspects of the reproductive performance of mature male Albino rats. For this purpose, a total of 100 mature male Albino rats were used. Rats were divided into 4 comparable groups; the first consists of 10 rats, was left as control. The second was administered glutamate 10 mg/ kg, the third group was injected by L- Arginine 20 mg/ kg while the fourth was injected by GABA 1 mg / rat. The results showed that administration of glutamate was concomitant with increase in synthesis and release of pituitary LH causing increase in its serum level as well as decrease serum level of testosterone. On the other hand, prolonged L-Arginine administration led to remarkable elevation in both pituitary and serum LH and significant decrease of serum testosterone. While, GABA administration led to remarkable decrease in pituitary and serum LH with significant decrease in serum testosterone level.

In 9 weeks field study, a total of 14100 (Ross broiler breeders) 51 weeks-old chickens fed on the same ration, placed in 2 houses (6600 female + 450 male / house). Birds of house 1 were treated with antimycotoxin adsorbent aluminum silicate (G-V-tox®) 5 kgm/ ton, while those of house 2 were kept as non treated controls. Productivity and reproductivety parameters were calculated for comparison. Treated flock showed improved average egg production (Average 62.2%/week) compared with non treated (Average 61.7%/week), but all still lower than farm stander (Average 76.4%/week). Marked improvement was in the 1st 3 weeks (51-53) of treatment only. Total 9 weeks production declined was 5.5% and 8.4% in control and silicate treated flock; with weekly average of 0.61, and 0.93; respectively. Control flock was slower in decline of production (0.61%/week) than treated flock (0.93%/week). Average weekly egg production and hatching eggs/ hen in treated flock was lower than standard and higher than non treated. Hatchery parameters of treated were improved in treated at the first 3 weeks post treatment. The fertility was higher in aluminum silicate treated group (77.2%), than the untreated one (72.19%). The hatchability was in silicate treated (63.66%) versus (62.25%) in the untreated control. Culls % in hatched chicks was 1.91% in treated flock and lower than in non treated (2.85%). Difference percentage between fertility and hatchability of G.V. tox treated chickens was (10.84%) higher than untreated control (16%). The number of marketable chicks l100 was also improved in treated than non treated. In conclusion, our field study cleared that administration of Silicate in ration for treatment of broiler breeders resulted in an improved production and hatchery performance as compared with non medicated control. However; it did not restore it to the farm stander. Consequently the results indicated that we still in need for more effective products to be used to control mycotoxins in breeder chicken.

To achieve a better diagnostic assay for the bovine tuberculosis, antigen cocktail composed of Ag85; rESAT-6 and rMPB-70 protein antigens were compared to the bovine tuberculin using ELISA test. The antigen cocktail could detect 80 % of the tuberculin negative cattle showed lesions in the post mortem examination compared to 60% with the PPD-B. For the tuberculin positive cattle, the results were 70% and 80% for the antigen cocktail and PPD-B respectively. On the other hand the antigen cocktail gave only 26.6% in sera of the tuberculin positive animals with non visible lesions compared to 53.3% with the PPD-B. 70% of the tuberculin negative cattle with localized lesions in the P/M examination reacted with the antigen cocktail compared to 50% only with the PPD-B. The results concluded the ability of the antigen cocktail to overcome the false negative results obtained with the tuberculin test and to detect the active recent infection of cattle which allow the rapid eradication of the infected animals from herds before the spread of the disease.

Fifteen samples of fish fillets were collected from different Egyptian markets to detect the residues of some heavy metals in them. The average weights of fish fillets samples were 250g. The samples were separately packed in polyethylene bag and sent to the laboratory for preparation and analysis. All equipment surfaces and utensils which used in this study were thoroughly cleaned with detergent, rinsed with water, dried and then rinsed with 2-propanol solution. The samples were stored at –2C prior to analysis. The mean levels of lead and cadmium in fish fillets collected from the Egyptian markets were 1.51  0.34 and 0.60 0.0.25 mg/kg; respectively. The concentration of lead in fish muscle were less than the permissible heavy metal limit in fish meat (2.0 mg kg) as stated by the World Health Organization, also these results were in line with the corresponding EU regulations. However the level of cadmium concentration was over than the heavy metal limits in fish meat (0.5 mg/kg) for the World Health Organization.

The antiviral activity of Curcuma longa against NDV on vero cell culture and in infected chicken was observed. The obtained results showed that low concentration of Curcuma longa 0.25% did not produce cytotoxicity on the vero cell. The effect of Curcuma longa on NDV titre was studied and the results revealed clearly that chickens treated with 1% Curcuma longa as Prophylactic also showed higher protection rate (90%), the chickens that treated with 0.5 % Curcuma longa showed protection rate (80%).Chicks that infected with NDV without treatment with Curcuma longa showed lower protection rate (8%). The chickens infected then treated with 1% Curcuma longa showed protection (65%) and the chickens infected then treated with 0.5% Curcuma longa showed protection (40%).

Tetanus toxoid for equines has been prepared by cultivation of C. tetani into new synthetic medium for production of high potent tetanus toxin which gave high flocculation units [90 Lf (Limit of flocculation)], and high minimum lethal dose (MLD) (600,000). Tetanus toxin was inactivated by formalin, and adsorbed on aluminum hydroxide gel as an adjuvant. It was inoculated into rabbits as a preliminary evaluation for its potency, showing the permissible units allowed to be used for vaccination of horse. The vaccine was tested in horses, which exhibited higher titer of specific antibodies persisted for 8 months post vaccination.

Toxocariasis is a disease of unspecific clinical manifestations in human beings and some animals forming a condition known as visceral larva migerans. Effective Toxocara canis (T. canis) control should destroy the dormant hypobiotic larvae in the tissues of the reservoir hosts. Embyonated egg antigen is intended to be evaluated in rabbits where15 New Zealand rabbits were divided into 3 groups; immunized group that injected with three doses of T. canis egg antigen (First dose with complete Freund's and two successive doses with incomplete Freund’s adjuvant); control infected group and adjuvant control group. At 30th day from the beginning of the experiment, all groups were infected with 10,000 T. canis embryonated egg. Blood samples were collected periodically for measuring antibodies titer by ELISA. Postmortem and histopathological investigations were done. The efficacy of egg antigen protective immunity was evaluated by; the positive antibody titer, retention of larvae in the liver, hepatic eosinophilic granuloma formation, number of the milky spots on the liver surface and lung resistance to the infection. These parameters were detected obviously in the immunized group than the non immunized ones. Consequently, production of this product is recommended where its preparation is simple, easy, fast and economic and may be used as role of vaccine program against the parasite infection in the susceptible hosts.

In this study, a combined Trivalent vaccine against ND, IB and M. gallisepticum was locally prepared and evaluated in comparison with other locally prepared Bivalent ND and IB and monovalent M. gallisepticum vaccines. The obtained results were promising for this locally prepared Trivalent vaccine and the immune response was outstanding starting at the 2nd week post vaccination and showed extended raising allover the experiment period. The immune response of chickens vaccinated with the Trivalent was shoot up post boostering at the 8th week post 1st vaccination. These results were confirmed and supported by the challenge tests using the virulent strains of the three pathogens. So it could be recommend that the production of this Trivalent ND, IB and M. gallisepticum will help in the control of the three diseases and their complications.

A natural outbreak of Bovine Ephemeral Fever in Egypt during the summer of 2006 had been observed. In Beni Suef province, out of 70 cattle naturally infected with bovine ephemeral fever virus, three fattening calves suffered from subcutaneous emphysema died and were subjected to post-mortem examination. The findings revealed severe subcutaneous emphysema, interstitial and pulmonary emphysema. The serous membranes were thick, opaque and emphysematous. Microscopically, interstitial and pulmonary emphysema was prominent in most lobes of the examined lungs accompanied with pulmonary oedema and focal leucocytic aggregations in some areas. Angiopathy was demonstrated in all cases. The bronchial and mediastinal lymph nodes showed congestion and hemorrhages. Immunohistochemically, specific reaction for Bovine Ephemeral Fever virus was demonstrated in the lung and lymph nodes of the three cases; the pathogenesis of the disease was discussed.

Out of 63 bacteriologically examined sanguineous fluids samples which were collected from Oedematous Skin Disease (O.S.D.) lesions, 37 isolates of C. pseudotuberculosis (C.p.) were recovered. The sensitivity tests revealed that isolates were highly sensitive to trimethoprim + sulphamethoxazole, amoxycillin, gentamicin and enrofloxacin. Diphtheria toxin (DT) produced by C. pseudotuberculosis of buffalo was determined by using the double immunodiffusion technique, it was applied on concentrated exotoxins which was prepared from C.p. isolates against Diphtheria toxin antiserum, its results were 15 (40.54%) positive and 22 (59.46) %) negative to presence of DT. Virulence of isolates having only phospholipase D ( PLD) or both PLD and DT was assayed by S/C inoculation of exotoxines prepared from isolates in guinea pigs, 15(40.54%) guinea pigs died within 18 hours while 22 (59.46%) guinea pigs died during 48 hours. An important point in this investigation that there have been very rare previous reports describing the production of DT by local isolates of C. pseudotuberculosis of buffalo.