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Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation 全文
2021
Jasik Agnieszka | Kycko Anna | Olech Monika | Wyrostek Krzysztof | Śmiech Anna | Łopuszyński Wojciech | Otrocka-Domagała Iwona | Mikiewicz Mateusz | Dolka Izabella
Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation 全文
2021
Jasik Agnieszka | Kycko Anna | Olech Monika | Wyrostek Krzysztof | Śmiech Anna | Łopuszyński Wojciech | Otrocka-Domagała Iwona | Mikiewicz Mateusz | Dolka Izabella
Apocrine sweat gland carcinomas (ASGCs) are rare malignant skin tumours in dogs and humans. The literature published so far focuses mostly on the clinico-epidemiological aspect of these tumours, but little is known about their pathogenesis. In this study we aimed to determine whether the p53 gene is involved in the carcinogenesis of the apocrine sweat gland in dogs and whether ultraviolet radiation (UV) is related to it.
显示更多 [+] 显示较少 [-]Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation 全文
2021
Jasik, Agnieszka | Kycko, Anna | Olech, Monika | Wyrostek, Krzysztof | Smiech, Anna | Łopuszyński, Wojciech | Otrocka-Domagała, Iwona | Mikiewicz, Mateusz | Dolka, Izabella
Apocrine sweat gland carcinomas (ASGCs) are rare malignant skin tumours in dogs and humans. The literature published so far focuses mostly on the clinico-epidemiological aspect of these tumours, but little is known about their pathogenesis. In this study we aimed to determine whether the p53 gene is involved in the carcinogenesis of the apocrine sweat gland in dogs and whether ultraviolet radiation (UV) is related to it. Forty canine ASGCs were submitted to laser capture microdissection to isolate neoplastic cells, from which DNA was subsequently extracted. PCR amplification and sequencing of p53 exons 2–8 was then performed, followed by computer analysis of the obtained sequences. Sixteen mutations within the p53 gene were found in 13 tumours. The mutations involved C → T, T → C, G → A, and CC → TT transitions, C → G transversion and adenine deletion, which are gene alteration types known to be related to UV radiation in the process of skin carcinogenesis in humans. Six of the thirteen tumour cases displayed the C → T transitions in the same location in exon 4 and three of the thirteen cases displayed T → C in the same location in exon 5. The results of the present study indicate both the participation of the p53 gene and the influence of UV radiation in the formation of ASGCs in dogs.
显示更多 [+] 显示较少 [-]Evaluation of serum and urine neutrophil gelatinase-associated lipocalin and cystatin C as biomarkers of acute kidney injury in horses 全文
2021
Siwińska Natalia | Żak Agnieszka | Pasławska Urszula
Evaluation of serum and urine neutrophil gelatinase-associated lipocalin and cystatin C as biomarkers of acute kidney injury in horses 全文
2021
Siwińska Natalia | Żak Agnieszka | Pasławska Urszula
Diagnosis of acute kidney injury (AKI) in horses is difficult at the subclinical stage, due to nonspecific clinical signs. The aim of this study was to evaluate the concentrations of selected serum and urinary biomarkers in healthy horses, horses at risk of AKI, and those with clinical AKI.
显示更多 [+] 显示较少 [-]Evaluation of serum and urine neutrophil gelatinase-associated lipocalin and cystatin C as biomarkers of acute kidney injury in horses 全文
2021
Siwińska, Natalia | Żak, Agnieszka | Pasławska, Urszula
Diagnosis of acute kidney injury (AKI) in horses is difficult at the subclinical stage, due to nonspecific clinical signs. The aim of this study was to evaluate the concentrations of selected serum and urinary biomarkers in healthy horses, horses at risk of AKI, and those with clinical AKI. Thirty healthy horses, 30 horses at risk of AKI and 11 horses with clinical AKI and azotaemia were included in the study. Serum and urinary neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C were measured using commercially available enzyme immunoassay tests. The median and (in parentheses) first and third quartile concentrations of selected biomarkers in healthy horses, horses at risk of AKI and horses with AKI were respectively as follows: serum cystatin C – 0.25 (0.19–0.37), 0.23 (0.15–0.37) and 0.61 (0.37–1.13) mg/L; serum NGAL – 50.5 (38.8–58.8), 51.1 (40.4–66.9) and 98.1 (59.4–128.2) ng/mL; urinary NGAL – 20.7 (17.9–24.5), 32.3 (32.7–55.8) and 36.6 (26.8–89.9) ng/mL; and urinary cystatin C – 0.1 (0.07–0.13), 0.13 (0.1–0.2) and 0.34 (0.22–0.37) mg/L. There were significant differences in the concentration of all biomarkers between the healthy and AKI-affected horses. Horses with AKI all had biomarker concentrations higher than the healthy horses. None of the biomarkers made azotaemia recognisable in all affected horses. The obtained results indicate the need to create a serum and urinary biomarker panel to detect AKI.
显示更多 [+] 显示较少 [-]A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model 全文
2021
Kim Young Kyu | Lee Ju Young | Hwang Jeong Ho | Suh Han Na
A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model 全文
2021
Kim Young Kyu | Lee Ju Young | Hwang Jeong Ho | Suh Han Na
Because minipig skin is similar to human skin in anatomy and physiology, establishing an atopic dermatitis (AD) minipig model seems meaningful.
显示更多 [+] 显示较少 [-]A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model 全文
2021
Kim, Yŏng-gyu | Lee, Ju Young | Hwang, Jeong Ho | Suh, Han Na
Because minipig skin is similar to human skin in anatomy and physiology, establishing an atopic dermatitis (AD) minipig model seems meaningful. We applied 1-fluoro-2,4-dinitrobenzene (DNFB) or ovalbumin onto the back skin of five Yucatan minipigs aged 8–10 months and 19 kg in median weight. Two minipigs with the same parameters served as controls. Both DNFB and ovalbumin mediated epithelial hyperplasia, spongiosis, and immune cell infiltration in the dermis, which is a typical histopathological feature of AD. Moreover, AD upregulated the Th1- and Th2-related cytokine expressions in DNFB- or in ovalbumin-treated skin. Notably, AD-induced minipigs exhibited greater cytokine serum concentrations. Histopathological finding and cytokine analysis revealed that DNFB or ovalbumin mediates AD. However, ovalbumin-treated minipig is a more reliable and precise AD model owing to the DNFB-induced severe skin damage. In summary, ovalbumin-treated skin shows similar AD as human in histopathological and molecular analysis.
显示更多 [+] 显示较少 [-]Regional distribution of non-human H7N9 avian influenza virus detections in China and construction of a predictive model 全文
2021
Huang Zeying | Li Haijun | Huang Beixun
Regional distribution of non-human H7N9 avian influenza virus detections in China and construction of a predictive model 全文
2021
Huang Zeying | Li Haijun | Huang Beixun
H7N9 avian influenza has broken out in Chinese poultry 10 times since 2013 and impacted the industry severely. Although the epidemic is currently under control, there is still a latent threat.
显示更多 [+] 显示较少 [-]Regional distribution of non-human H7N9 avian influenza virus detections in China and construction of a predictive model 全文
2021
Huang, Zeying | Li, Haijun | Huang, Beixun
H7N9 avian influenza has broken out in Chinese poultry 10 times since 2013 and impacted the industry severely. Although the epidemic is currently under control, there is still a latent threat. Epidemiological surveillance data for non-human H7N9 avian influenza from April 2013 to April 2020 were used to analyse the regional distribution and spatial correlations of positivity rates in different months and years and before and after comprehensive immunisation. In addition, positivity rate monitoring data were disaggregated into a low-frequency and a high-frequency trend sequence by wavelet packet decomposition (WPD). The particle swarm optimisation algorithm was adopted to optimise the least squares support-vector machine (LS-SVM) model parameters to predict the low-frequency trend sequence, and the autoregressive integrated moving average (ARIMA) model was used to predict the high-frequency one. Ultimately, an LS-SVM-ARIMA combined model based on WPD was constructed. The virus positivity rate was the highest in late spring and early summer, and overall it fell significantly after comprehensive immunisation. Except for the year 2015 and the single month of December from 2013 to 2020, there was no significant spatiotemporal clustering in cumulative non-human H7N9 avian influenza virus detections. Compared with the ARIMA and LS-SVM models, the LS-SVM-ARIMA combined model based on WPD had the highest prediction accuracy. The mean absolute and root mean square errors were 2.4% and 2.0%, respectively. Low error measures prove the validity of this new prediction method and the combined model could be used for inference of future H7N9 avian influenza virus cases. Live poultry markets should be closed in late spring and early summer, and comprehensive H7N9 immunisation continued.
显示更多 [+] 显示较少 [-]Occurrence of selected pathogenic microorganisms in raw and processed eggs of snails of the Cornu genus 全文
2021
Maćkowiak-Dryka Monika | Gondek Michał | Szkucik Krzysztof
Occurrence of selected pathogenic microorganisms in raw and processed eggs of snails of the Cornu genus 全文
2021
Maćkowiak-Dryka Monika | Gondek Michał | Szkucik Krzysztof
This study investigated the eggs of Polish-bred edible snails of the Cornu genus as a food and aimed to determine the presence of microorganisms in them of the Salmonella and Listeria genera and ascertain the number of coagulase-positive staphylococci.
显示更多 [+] 显示较少 [-]Occurrence of selected pathogenic microorganisms in raw and processed eggs of snails of the Cornu genus 全文
2021
Maćkowiak-Dryka, Monika | Gondek, Michał | Szkucik, Krzysztof
This study investigated the eggs of Polish-bred edible snails of the Cornu genus as a food and aimed to determine the presence of microorganisms in them of the Salmonella and Listeria genera and ascertain the number of coagulase-positive staphylococci. Raw material, semi-finished products, and the final product were collected during the production cycle. Testing for the presence of Salmonella spp. and Listeria spp. and measuring of the pathogenic staphylococci contamination level were carried out in accordance with ISO standards. Commercial biochemical tests were used for species identification of bacteria of the Enterobacteriaceae family and Staphylococcus genus. An API kit and a PCR protocol were utilised for species confirmation of the microorganisms of the Listeria genus. Neither Salmonella nor coagulase-positive staphylococci were found in any of the studied material. Bacteria of the Listeria genus were found in samples taken at every stage of production; however L. monocytogenes was confirmed in samples of the final product. The absence of Salmonella spp. and Staphylococcus aureus in samples of the final product indicates that the required hygiene standard was maintained in the production process of edible snail eggs. Nevertheless, the presence of L. monocytogenes in eggs of common garden snails may pose a potential risk to consumer health.
显示更多 [+] 显示较少 [-]Balantidium coli in pig farms suspected of porcine circovirus type 2 (PCV2) associated enteritis 全文
2021
Szczotka-Bochniarz Anna | Karamon Jacek | Nowak Agnieszka | Porowski Marian | Karbowiak Paweł | Holeniewski Andrzej | Pejsak Zygmunt
Balantidium coli in pig farms suspected of porcine circovirus type 2 (PCV2) associated enteritis 全文
2021
Szczotka-Bochniarz Anna | Karamon Jacek | Nowak Agnieszka | Porowski Marian | Karbowiak Paweł | Holeniewski Andrzej | Pejsak Zygmunt
Diarrhoea in growing-finishing pigs is a common problem of commercial pig farms. Among many causative factors, porcine circovirus type 2 (PCV2) is one considered an important pathogen in modern pig production. The aim of the study was to verify if PCV2 was responsible for antibiotic non-responsive diarrhoea and wasting in pigs.
显示更多 [+] 显示较少 [-]Balantidium coli in pig farms suspected of porcine circovirus type 2 (PCV2) associated enteritis
Balantidium coli in pig farms suspected of porcine circovirus type 2 (PCV2) associated enteritis 全文
2021
Szczotka-Bochniarz, Anna | Karamon, Jacek | Nowak, Agnieszka | Porowski, Marian | Karbowiak, Paweł | Holeniewski, Andrzej | Pejsak, Zygmunt
Diarrhoea in growing-finishing pigs is a common problem of commercial pig farms. Among many causative factors, porcine circovirus type 2 (PCV2) is one considered an important pathogen in modern pig production. The aim of the study was to verify if PCV2 was responsible for antibiotic non-responsive diarrhoea and wasting in pigs. A total of 13 dead pigs aged between 12 and 15 weeks from three Polish farms with persistent herd symptoms suggestive of PCV2 infection were provided for evaluation. Sections of lymph nodes and intestines were analysed by in situ hybridization (ISH) for PCV2 and histopathological examination. Faeces and intestinal scrapings were tested for Lawsonia intracellularis and Brachyspira hyodysenteriae by real-time PCR and for parasitic infection by flotation and decantation. ISH and histopathological examination showed that all pigs were PCV2 systemic disease negative. Swine dysentery was confirmed by real-time PCR on two farms, and proliferative enteropathy on one farm. In histological examinations, erosions of the caecal and colonic mucosa were found, together with cysts and trophozoites of Balantidium coli. The protozoa were present in the intestinal lumen and mucosa. B. coli cysts were identified in faeces from all examined pigs. These results suggest that monitoring of B. coli infections should be an additional measure of control and prevention of gastrointestinal tract disorders in modern swine husbandry.
显示更多 [+] 显示较少 [-]Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue 全文
2021
Ceccopieri Cassandra | Skonieczna Joanna | Madej Jan P.
Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue 全文
2021
Ceccopieri Cassandra | Skonieczna Joanna | Madej Jan P.
The aim of our study was to optimise an existing staining procedure: haematoxylin-eosin saffron (HES). The method follows the classical haematoxylin and eosin protocol with the addition of a staining step using natural saffron to better identify the collagen fibres.
显示更多 [+] 显示较少 [-]Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue 全文
2021
Ceccopieri, Cassandra | Skonieczna, Joanna | Madej, Jan P.
The aim of our study was to optimise an existing staining procedure: haematoxylin-eosin saffron (HES). The method follows the classical haematoxylin and eosin protocol with the addition of a staining step using natural saffron to better identify the collagen fibres. The saffron solution was obtained by dissolving ground saffron stigmas in absolute alcohol. In order to test the HES method for its staining ability on four main types of collagen (I, II, III, and IV), specific tissues (skin, tooth, cartilage, aorta, spleen, and penis) were chosen. The procedure showed a sharp differentiation between muscle, stained red or pink, and connective tissue, stained bright yellow or orange. HES allows the diagnosis of reticulin fibrosis undetected in HE and in previous saffron staining procedures. HES represents an advantageous alternative to HE staining giving highly reproducible results with high diagnostic value.
显示更多 [+] 显示较少 [-]Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae 全文
2021
Huang Dean | Chao Yu-Chan | Lv Zhengbing | Jan Jia-Tsrong | Yang Yu-Chih | Hsiao Pei-Wen | Wu Chia-Ying | Liao Chiu-Hsun | Wu Tzu-Hsien | Wang Lih-Chiann
Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae 全文
2021
Huang Dean | Chao Yu-Chan | Lv Zhengbing | Jan Jia-Tsrong | Yang Yu-Chih | Hsiao Pei-Wen | Wu Chia-Ying | Liao Chiu-Hsun | Wu Tzu-Hsien | Wang Lih-Chiann
Novel clade 2.3.4.4 H5 highly pathogenic avian influenza virus (HPAIV) outbreaks have occurred since early 2015 in Taiwan and impacted the island economically, like they have many countries. This research investigates the immunogenicity of two HPAIV-like particles to assess their promise as vaccine candidates.
显示更多 [+] 显示较少 [-]Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae 全文
2021
Huang, Dean | Chao, Yu-Chan | Lv, Zhengbing | Jan, Jia-Tsrong | Yang, Yuzhi | Hsiao, Pei-Wen | Wu, Jiaying | Liao, Chiu-Hsun | Wu, Tzu-Hsien | Wang, Lih-Chiann
Novel clade 2.3.4.4 H5 highly pathogenic avian influenza virus (HPAIV) outbreaks have occurred since early 2015 in Taiwan and impacted the island economically, like they have many countries. This research investigates the immunogenicity of two HPAIV-like particles to assess their promise as vaccine candidates. The haemagglutinin (HA) gene derived from clade 2.3.4.4 H5 HPAIV and matrix protein 1 (M1) gene were cloned into the pFastBac Dual baculovirus vector. The resulting recombinant viruses were expressed in Spodoptera frugiperda moth (Sf)21 cells and silkworm pupae to generate Sf21 virus-like particles (VLP) and silkworm pupa VLP. Two-week-old specific pathogen–free chickens were immunised and their humoral and cellular immune responses were analysed. The silkworm pupa VLP had higher haemagglutination competence. Both VLP types elicited haemagglutination inhibition antibodies, anti-HA antibodies, splenic interferon gamma (IFN-γ) and interleukin 4 (IL-4) mRNA expression, and CD4⁺/CD8⁺ ratio elevation. However, chickens receiving silkworm pupa VLP exhibited a significantly higher anti-HA antibody titre in ELISA after vaccination. Although Sf21 VLP recipients expressed more IFN-γ and IL-4, the increase in IFN-γ did not significantly raise the CD4⁺/CD8⁺ ratio and the increase in IL-4 did not promote anti-HA antibodies. Both VLP systems possess desirable immunogenicity in vivo. However, in respect of immunogenic efficacy and the production cost, pupa VLP may be the superior vaccine candidate against clade 2.3.4.4 H5 HPAIV infection.
显示更多 [+] 显示较少 [-]Phytoncides in the prevention and therapy of blackhead disease and their effect on the turkey immune system 全文
2021
Tykałowski Bartłomiej | Śmiałek Marcin | Kowalczyk Joanna | Dziewulska Daria | Stenzel Tomasz | Koncicki Andrzej
Phytoncides in the prevention and therapy of blackhead disease and their effect on the turkey immune system 全文
2021
Tykałowski Bartłomiej | Śmiałek Marcin | Kowalczyk Joanna | Dziewulska Daria | Stenzel Tomasz | Koncicki Andrzej
Turkey histomonosis poses a serious threat to poultry production due to the ban on the use of effective drugs. The aim of the study was to evaluate the influence of a phytoncidal feed supplement on the course of histomonosis. The preparation was also analysed for immunomodulatory properties.
显示更多 [+] 显示较少 [-]Phytoncides in the prevention and therapy of blackhead disease and their effect on the turkey immune system 全文
2021
Tykałowski, Bartłomiej | Śmiałek, Marcin | Kowalczyk, Joanna | Dziewulska, Daria | Stenzel, Tomasz | Koncicki, Andrzej
Turkey histomonosis poses a serious threat to poultry production due to the ban on the use of effective drugs. The aim of the study was to evaluate the influence of a phytoncidal feed supplement on the course of histomonosis. The preparation was also analysed for immunomodulatory properties. Clinical observations and production monitoring were conducted in a flock of turkeys with histomonosis from their 11ᵗʰ to 56ᵗʰ weeks of life which were treated with the adiCoxSᴼᴸPF soluble supplement in a dose of 2.5 mL/L water. Later the preparation was used in a preventive dose (1 mL/L). The influence on the immune system was evaluated in broiler turkeys having been given adiCoxSᴼᴸPF for 3 days in doses of 1 or 3 mL/L. The T and B lymphocyte percentages in turkey blood and spleen tissue were analysed with flow cytometry. ELISA was implemented to evaluate antibody titres after Ornithobacterium rhinotracheale vaccination, and biochemical analyses were performed to evaluate the supplement’s safety. AdiCoxSᴼᴸPF was found effective in therapy and prevention of histomonosis. Additionally, adiCoxSᴼᴸPF stimulated both humoral and cell-mediated immune mechanisms, without impairing the functions of internal organs. The treated turkeys also yielded better production results (eggs/hen, fertility, and hatchability). AdiCoxSᴼᴸPF possesses immunomodulatory properties and it can be used successfully in the prevention and therapy of histomonosis in turkeys.
显示更多 [+] 显示较少 [-]Mink SARS-CoV-2 infection in Poland – short communication 全文
2021
Domańska-Blicharz Katarzyna | Orłowska Anna | Smreczak Marcin | Niemczuk Krzysztof | Iwan Ewelina | Bomba Arkadiusz | Lisowska Anna | Opolska Justyna | Trębas Paweł | Potyrało Patrycja | Kawiak-Sadurska Magdalena | Rola Jerzy
Mink SARS-CoV-2 infection in Poland – short communication 全文
2021
Domańska-Blicharz Katarzyna | Orłowska Anna | Smreczak Marcin | Niemczuk Krzysztof | Iwan Ewelina | Bomba Arkadiusz | Lisowska Anna | Opolska Justyna | Trębas Paweł | Potyrało Patrycja | Kawiak-Sadurska Magdalena | Rola Jerzy
Since April 2020, when the first SARS-CoV-2 infection was reported in mink and subsequently in mink farm workers in the Netherlands, it has been confirmed that human-to-mink and mink-to-human transmission can occur. Later, SARS-CoV-2 infections in mink were reported in many European and North American countries.
显示更多 [+] 显示较少 [-]Mink SARS-CoV-2 infection in Poland – short communication 全文
2021
Domańska-Blicharz, Katarzyna | Orłowska, Anna | Smreczak, Marcin | Niemczuk, Krzysztof | Iwan, Ewelina | Bomba, Arkadiusz | Lisowska, Anna | Opolska, Justyna | Trębas, Paweł | Potyrało, Patrycja | Kawiak-Sadurska, Magdalena | Rola, Jerzy
Since April 2020, when the first SARS-CoV-2 infection was reported in mink and subsequently in mink farm workers in the Netherlands, it has been confirmed that human-to-mink and mink-to-human transmission can occur. Later, SARS-CoV-2 infections in mink were reported in many European and North American countries. Samples from 590 mink from a total of 28 farms were tested by real-time RT-PCR. Whole genome sequences from one positive farm were generated and genetic relatedness was established. SARS-CoV-2 RNA was detected on a breeder farm with stock of 5,850 mink. Active viraemia was confirmed in individually tested samples with Ct values respectively between 19.4 and 29.6 for E and N gene fragments. Further testing of samples from culled animals revealed 70% positivity in throat swabs and 30% seropositivity in blood samples. Phylogenetic analysis of full-length nucleotide sequences of two SARS-CoV-2 isolates revealed that they belong to the 20B Nextstrain clade. Several nucleotide mutations were found in analysed samples compared to the reference Wuhan HU-1 strain and some of them were nonsynonymous. We report the infection of mink with SARS-CoV-2 on one farm in Poland and the results of subsequent analysis of virus sequences from two isolates. These data can be useful for assessment of the epidemiological situation of SARS-CoV-2 in Poland and how it endangers public health.
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