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Establishment of a new canine inflammatory mammary carcinoma cell line and analysis of its cystine-glutamate transporter subunit expression 全文
2022
Itoh Harumichi | Naruse Ryo | Tani Kenji | Sunahara Hiroshi | Nemoto Yuki | Nakaichi Munekazu | Iseri Toshie | Horikirizono Hiro | Itamoto Kazuhito
Inflammatory mammary carcinoma (IMC) is a rare disease with a poor prognosis and one affecting dogs. Inflammatory breast carcinoma (IBC) is a subtype of malignant breast cancer in humans with a high degree of malignancy and a similarly poor prognosis. Since the clinical symptoms and prognoses of both are similar, canine IMC has been considered as a model of human IBC. In this study, we newly established a stable IMC-derived cell line from a patient at the Yamaguchi University Animal Medical Center in Japan.
显示更多 [+] 显示较少 [-]Effective control of Johne’s disease in large Czech dairy herds 全文
2022
Fichtelová Věra | Králová Alena | Babák Vladimír | Kovařčík Kamil
Johne’s disease, caused by infection with Mycobacterium avium subsp. paratuberculosis (MAP), causes economic losses in dairy herds due to reduced milk production and premature culling. A test-and-cull strategy coupled with changes in calf rearing management preventing new infections has been introduced into infected herds to control MAP prevalence. This study appraised the effectiveness of these practice changes.
显示更多 [+] 显示较少 [-]Simultaneous determination of pyrrolizidine and tropane alkaloids in honey by liquid chromatography–mass spectrometry 全文
2022
Kowalczyk Ewelina | Kwiatek Krzysztof
Pyrrolizidine alkaloids (PAs) and tropane alkaloids (TAs) are natural contaminants of honey and respectively hepatoxic and neurotoxic compounds. Because honey is a popular constituent of the human diet, it is relevant to warrant the safety of the product. For that reason, a method for simultaneous determination of PAs and TAs in honey based on liquid chromatography– mass spectrometry was developed.
显示更多 [+] 显示较少 [-]Characterisation of ORF3, M, N and E gene sequences of porcine epidemic diarrhoea virus from domestic pigs in Poland 全文
2022
Olech Monika | Antas Marta | Szczotka-Bochniarz Anna
Porcine epidemic diarrhoea virus (PEDV) is an enteric pathogen causing porcine epidemic diarrhoea and acute gastroenteritis in pigs of all ages. Previous analysis of the viral genome of PEDV in Poland was only based on the spike protein (S) gene sequences and no analysis of other genes has been performed. The aim of this study was to analyse the envelope (E), membrane (M) and nucleocapsid (N) protein and open reading frame 3 (ORF3) gene sequences.
显示更多 [+] 显示较少 [-]Prevalence of tumours and tumour-like lesions in domestic and exotic animals from Lower Silesia and its surrounds in Poland in 2014–2017 全文
2022
Ciaputa Rafał | Gach Justyn | Baranowski Karol | Dzimira Stanisław | Janus Izabela | Kandefer-Gola Małgorzata | Żebrowski Kacper | Nowak Marcin
An analysis of samples originating from domestic and exotic animals from Lower Silesia but also from other parts of Poland was carried out based on research conducted in 2014–2017.
显示更多 [+] 显示较少 [-]Two centrifugal flotation techniques for counting gastrointestinal parasite eggs and oocysts in alpaca faeces 全文
2022
Maria Pyziel-Serafin Anna | Raboszuk Adrianna | Klich Daniel | Orłowska Blanka | Sierociuk Dagmara | Anusz Krzysztof
The alpaca (Vicugna pacos) is a camelid native to South America, but the species has wide distribution outside its natural habitat and is found in various countries on other continents, Poland being one in Europe. Parasitic infections affect the productivity and health of alpacas. The aim of the study was to estimate the parasite loads in alpacas kept in Poland with the use of two direct centrifugal flotation methods.
显示更多 [+] 显示较少 [-]The occurrence of Fascioloides magna (Bassi, 1875) in the wild cervid population in the Lower Silesian Wilderness – epidemiological and pathological aspects 全文
2022
Filip-Hutsch Katarzyna | Pyziel-Serafin Anna M. | Hutsch Tomasz | Bulak Kamila | Czopowicz Michał | Merta Dorota | Kobielski Janusz | Demiaszkiewicz Aleksander W.
Fascioloides magna is a parasite of high veterinary importance due to its pathogenicity for wild and domestic ruminants. The aim of our study was to describe the presence of trematode infection in the red deer population in the Lower Silesian Wilderness, one of the established fascioloidosis foci in Central Europe, and to assess the overall prevalence of F. magna in the studied area. In order to achieve this, a coprological study of different cervid species was performed.
显示更多 [+] 显示较少 [-]The potential risk of international spread of Mycobacterium bovis associated with movement of alpacas 全文
2022
Krajewska-Wędzina Monika | Miller Michele A. | Didkowska Anna | Kycko Anna | Radulski Łukasz | Lipiec Marek | Weiner Marcin
The study highlights the transboundary nature of tuberculosis (TB) in alpacas and the failure of current ante-mortem testing protocols (the tuberculin skin and Enferplex Camelid TB tests) to identify TB-free alpaca herds and individuals for export. Our research and the available literature indicate that the alpaca (Vicugna pacos) is extremely susceptible to Mycobacterium bovis infection, and that testing periodicity fails to take into account that animals do not manifest disease symptoms for a long time. The skin test failed to identify Mycobacterium bovis infection in two alpacas prior to their movement from the UK to Poland. The animals were purchased by a breeding centre in Poland, and were then shown at an international animal exhibition. The last owner of the alpacas before their deaths from TB bought the infected animals unwittingly in order to run rehabilitation activities with disabled children on his farm.
显示更多 [+] 显示较少 [-]Proteomic analysis of tear film in canine diabetic patients with and without retinopathy 全文
2022
Winiarczyk Dagmara | Winiarczyk Mateusz | Balicki Ireneusz | Szadkowski Mateusz | Michalak Katarzyna | Winiarczyk Stanisław | Adaszek Łukasz
Diabetic retinopathy (DR) is the leading cause of blindness in human and animal patients. Early detection and treatment of the disease are important and can be facilitated by proteomic approaches providing biomarkers.
显示更多 [+] 显示较少 [-]Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus–infected dams 全文
2022
Kaba Jarosław | Czopowicz Michał | Witkowski Lucjan | Szaluś-Jordanow Olga | Mickiewicz Marcin | Markowska-Daniel Iwona | Puchała Ryszard | Bagnicka Emilia
Small ruminant lentivirus (SRLV) causes caprine arthritis-encephalitis in goats and maedi-visna disease in sheep. Transmission is via ingestion of colostrum and milk from infected dams or long-term direct contact between animals. Lifelong seroconversion can occur several weeks after infection via ingestion. However, sub-yearling lambs that ingest contaminated colostrum may be able to clear the infection and become seronegative. Whether a similar phenomenon occurs in goats remains unknown. Therefore, the serological status of goats was studied longitudinally from the moment of natural exposure to colostrum and milk of SRLV-positive dams through the age of 24 months.
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