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AN IN SILICO APPROACH FOR THE EVALUATION OF CELL WALL COMPONENTS OF PROBIOTICS AS BIOLOGICAL ADSORBERS OF AFLATOXINS
2023
P. Jalantha | Ganne Venkata Sudhakar Rao | N. Pazhanivel | M. Parthiban | P. Veeramani | M.R. Srinivasan | C. Soundararajan
Aflatoxins are produced in poultry feed by two major fungal species viz., Aspergillus flavus and Aspergillus parasiticus during hot and humid seasons. To detoxify these aflatoxins, various binders are being used in the field which are mostly synthetic compounds with possible other toxicities, the harmless beneficial probiotics are being considered as binders to neutralize the effect of aflatoxins in the feed. Bacterial organisms (probiotics) like Lactobacillus rhamnosus, Lactobacillus casei, Bacillus subtilis, Enterococcus faecium and yeast (Saccharomyces cerevisiae) were selected and their cell wall structures have been retrieved and molecular docking was performed against four types of aflatoxins namely AFB1, AFB2, AFG1 and AFG2. The results revealed that cell wall components of the chosen bacterial organisms have a good binding affinity towards four types of aflatoxins. Based on this in silico results, it is evident that the both the lipoteichoic acid (LTA) and wall teichoic acid (TA) in the cell wall of probiotic organisms is responsible for its binding against aflatoxins.
显示更多 [+] 显示较少 [-]ANALYSING THE MANAGEMENT PRACTICES OF BACKYARD GOAT PRODUCTION IN CAUVERY DELTA ZONE OF TAMIL NADU
2023
R. Senthil | G. Senthil Kumar | A. Serma Saravana Pandian | Thanga Thamil Vanan
An attempt was made to document the management practices of the backyard goat production in Cauvery Delta zone of Tamil Nadu. The primary data were collected by interviewing 180 backyard goat farmers selected by multi-stage random sampling using pre-tested interview schedule and analyzed through descriptive statistics. About 46.11 per cent of the respondents kept their animals under open system of housing and floor of the goat shed was mud type in 96.67 per cent of the sampled farmers. All the farmers stored manure in an open place. Majority of goat farmers raise their goats solely on browsing and grazing stubble of field crops, natural pasture and tree leaves. Majority of farmers did not possess their own breeding buck but they use community buck for breeding purpose. Middlemen (57.22 per cent) played a major role in marketing of goat in all categories of flocks, followed by butcher (36.11 per cent). Only one-fifth of flock owners dewormed their flocks yearly twice. None of the sample respondents vaccinated their goat against infectious diseases. Goats were marketed round the year (87.78 per cent) and about 12 per cent marketed their goats on special occasions. The major reasons for marketing was found to be the want of money to overcome financial problem (61.07 mean score) in case of bucks / male kids and culling due to old age (66.00 mean score) in case of breeding does. Majority of the sample backyard goat farmers did not follow scientific management practices at their farm level, which warrants intensive extension programmes. Implementation of best management practices among backyard goat farmers in Cauvery Delta zone of Tamil Nadu would improve the profitability.
显示更多 [+] 显示较少 [-]UTILIZATION OF LIVESTOCK FARM SERVICES PROVIDED BY AGRICULTURAL TECHNOLOGY INFORMATION CENTRE (ATIC) OF TAMIL NADU VETERINARY AND ANIMAL SCIENCES UNIVERSITY
2023
M. Arul Prakash | R. Divisha
The objective of the present study is to find out the scope and extent of utilization livestock farm service provided by the Agricultural Technology Information Center (ATIC), TANUVAS, Kattuppakam, Chengalpattu district of Tamil Nadu. The available records for a period of two years from January 2021 to December 2022 were analysed to study the number of farmers visited, and their purpose of visits at ATIC, Kattupakkam. The findings of the study revealed that a total of 1727 farmers visited ATIC, Kattupakkam in which majority (86.21%) respondents were males. Among the specific purpose of visits, purchase of fodder seeds (35.95%) was their high priority followed by purchase of publications (32.42%), farm advisory (13.60%), livestock feed (11.92%) and others (6.07%). On farm advisory, majority were benefited on goat farming (31.66%) than other livestock farming practices. It was concluded that during the study period more than 65% of farmers benefited through the purchase for fodder seeds and publication and around 68% of the livestock farmers were likely to be more specific in their purpose of visits at ATIC, Kattupakkam.
显示更多 [+] 显示较少 [-]CHEMICAL COMPOSITION OF COMMON MEDICINAL HERBS USED FOR INDIAN TRADITIONAL MEDICINE
2023
R. Kavitha | C. Valli | R. Karunakaran | K. Vijayarani | R. Amutha
A total of sixteen commonly available medicinal herbs (different parts) used in Indian traditional medicine were collected. The collected parts were, shade-dried, ground to fine powder and stored in air-tight containers. The samples were analyzed for proximate principles, acid-insoluble ash, gross energy, minerals and fatty acids. Emblica officinalis fruits had significantly (P < 0.05) highest moisture (88.67%), Moringa oleifera leaves had significantly (P < 0.05) highest crude protein (26.99 %), Coriandrum sativum seeds had significantly (P < 0.05) highest crude fibre (32.53%), Syzygium aromaticum buds had significantly (P < 0.05) highest crude fat (18.57 %), Mentha spicata leaves had significantly (P < 0.05) highest total ash (11.53 %), Allium sativum bulbs had significantly (P < 0.05) highest nitrogen-free extracts (90.14 %) and Murraya koenigii leaves had significantly (P< 0.05) highest acid insoluble ash (4.71 %). The gross energy content was significantly (P < 0.05) highest in Coriandrum sativum seeds (4605.33 Kcal/Kg). Azadirachta indica leaves had significantly (P < 0.05) highest calcium (2.99 %), Phyllanthus niruri leaves had significantly highest (P < 0.05) phosphorus (0.53 %), Andrographis paniculata stem and leaves had significantly (P < 0.05) highest copper (41.27 ppm), E. officinalis fruits had significantly (P < 0.05) highest iron (596.78 ppm) and S. aromaticum buds had significantly (P < 0.05) highest manganese (286.96 ppm). C. sativum seeds had significantly (P < 0.05) highest levels of oleic acid (73.76 %), T. foenum seeds had significantly (P < 0.05) highest levels of linoleic acid (36.43 %) and P. niruri leaves had the significantly (P < 0.05) highest levels of linolenic acid (51.78 %) Further studies are needed to quantify other bioactive compounds, to study their antioxidant and antimicrobial properties so that they could be used as alternatives to antibiotics / other drugs for livestock and poultry.
显示更多 [+] 显示较少 [-]A NOVEL CASE REPORT ON APLASIA CUTIS CONGENITA CO-EXISTENCE WITH ABLEPHARON MACROSTOMIA SYNDROME (AMS) IN A NEW BORN BUFFALO CALF
2023
M. Praveen Kumar | M. Srinivas | B.V.S. Bhavya Charitha
The present case describes the successful vaginal delivery of a buffalo calf with Aplasia Cutis Congenita co-existence with Ablepharon Macrostomia Syndrome (AMS) in a graded Murrah buffalo.
显示更多 [+] 显示较少 [-]REGIONAL MASTECTOMY DUE TO MAMMARY ADENOMA IN A LABRADOR BITCH
2023
R. Kavin | R.M. Gowtham | T. Kavin | S. Pradeep
Mammary tumors are common in intact female animals. A nine year old intact, Labrador bitch was presented with a history of progressive swelling on the right inguinal mammary glands. Upon physical examination, it was tentatively diagnosed as a mammary tumor and confirmed with fine needle aspiration cytology as a mammary adenoma. A regional mastectomy was performed to remove the affected glands along with right inguinal lymph node. The bitch made an uneventful recovery with the resolution of clinical signs.
显示更多 [+] 显示较少 [-]ASSESSMENT OF THE BACTERIOLOGICAL QUALITY OF LOCALLY FERMENTED COW MILK (NONO) IN MAIDUGURI, NORTH EASTERN NIGERIA
2023
A. Bello | E. F. Ejeh | F. A. Lawan | A. A. Bitrus
Contamination of locally fermented cow milk (Nono) by pathogenic bacteria constitute a major public health problem. This was because contaminated milk was a good medium that can support the growth and propagation of microbial pathogens. This study was designed to assess the bacteriological quality of locally fermented cow milk consumed in Maiduguri, northeastern Nigeria. Totally, forty (n = 40) samples of fresh, locally fermented cow milk (Nono) were collected and used in this study. Ten (10) milk samples each were collected from four randomly selected selling points (Monday Market, Custom Market, Kasuwan Shanu and Tashan Bama). The bacteriological quality of each milk sample were analysed using standard procedures for isolation, identification and enumeration of pathogenic bacteria. The mean total plate count ranged from 1.44x10 8 CFU/mL to 10.31x10 8 CFU/mL, while the mean total coliform count range from 0.57x10 8 CFU/mL to 11.17x10 8 CFU/mL. Statistically, there was a significant difference (p<0.05) in the mean total plate count and coliform count of milk samples collected from the four sampling points.Bacteriological culture and biochemical identification of all the isolates revealed Escherichia coli29(72.5%), Klebsiella specie3(7.5%), Salmonella specie 2(5%), Staphylococcus aureus 14(35%) and Streptococcus specie15(37.5%) respectively. All the results were above the acceptable limits (1.0 to 3.0 x 10 5 CFU/mL for total plate count and no coliform count per 100ml of milk sample) set by NAFDAC. Therefore the Nono products consumed in Maiduguri was not safe and wholesome for public consumption. Hence, the need to ensure the maintenance of standard hygienic protocols during the collection, storage, processing and marketing of locally fermented cow milk (Nono) in order to minimize microbial contamination.
显示更多 [+] 显示较少 [-]EXVIVO STUDIES ON PHARMACODYNAMIC INTERACTION OF PHYTOCHEMICALS WITH ANTIBIOTICS AGAINST CLINICAL ISOLATES FROM MASTITIC MILK SAMPLES
2023
G. Srividya | B. Deepthi | S. Lakshminarasaiah | G. Srinivasarao
Drug interaction is the effect produced by combination of various drugs administered in a biological system which may produce beneficial or adverse effects.Due to continuous usage of antibiotics to bacterial infections, pathogenic bacteria developed resistance mechanisms which leads to reduced antibacterial activity of different antibiotics. Combination of antibiotics with phytochemicals became an alternative to enhance the efficacy of antibiotics. In this connection,in the present study, the effect of certain phytochemicals namely Quercitin, Morin, Ellagic acid, Chloragenic acid Rutin and Naringenin in combination with antibiotics Amoxycillin and Ciprofloxacin were evaluated. Staphylococcus aures and Escherichia coli were used as test organisms. They were isolated from the mastitis milk samples and their presence was confirmed using Polymerse Chain Reaction .The antibacterial activity of the antibiotics and antibiotics in combination with phytochemicals were determined using broth dilution method and agar well diffusion assay. The MIC and zones of inhibition were used as indicators for drug interaction.
显示更多 [+] 显示较少 [-]Effect of feeding enzyme mixture with Fibrolytic activity in dry and solubulized Forms on the performance of lambs
2023
M.V.A.N. Suryanarayana | J. V. Ramana
One hundred lambs (11.8 kg ± 0.56) divided at random into 4 groups G1, G2, G3 and G4 were supplemented with exogenous fibrolytic enzyme mixture (EFE) in concentrates after 6 hours outside grazing. G1 and G2 were given 200 and 400 g of EFE per 50 kg feed in dry form, G3 and G4 were given EFE in solubulized form at 20 and 40 g per litre of water, respectively. The initial and final weights (kg) were non-significant among treatments. The total weight gain (kg) was higher (P<0.05) for G4 and G2 as compared to other two. Average daily gain (g) was highest (P<0.05) for G4 followed by G2, G1 and G3. Total weight gain (%) is 15, 7.6 and 16.5 lower (P<0.05) for G1, G2 and G3 (Table) as compared to G4. Lowest (P<0.05) FCR was observed for G4 and is in the order of G4< G2< G1< G3. The cost of the feed per kg gain was lower (P<0.05) by 21%, 26% and 13% for G4 as compared to G1, G2 and G3. It can be concluded that addition of EFE in solubulized form was found better for the reason that it forms a stable complex with the feed and thus facilitates for the rumen bacterial attachment earlier for rapid action due to an increase in rumen bacterial population
显示更多 [+] 显示较少 [-]Studies on fresh milt parameters and cryopreservation of spermatozoa of endangered, golden mahseer, tor putitora (hamilton)
2023
Ravindragouda Patil | Wazir Singh Lakra | Shrinivas Jahageerdar | Gopal Krishna | Asim Kumar Pal
Physico-chemical parameters of fresh milt and mitochondrial activity of fresh spermatozoa of the endangered golden mahseer, Tor putitora (Hamilton) were investigated. Cryopreservation and fertility evaluation of cryopreserved-thawed spermatozoa of T. putitora were carried out. The concentration of spermatozoa and the spermatocrit values of the fresh milt were estimated to be 3.96±0.12 x 107 spermatozoa/ml and 70.00±1.53% respectively. The motility percentage of spermatozoa in the fresh milt was estimated to be 94.84±0.43. The elemental composition analysis revealed that the concentration of K+ was higher i.e., 14.13±0.32 mg/l when compared to that of Na+, Ca+, Mg+ and Zn+. The concentration of total reducing sugars and total proteins were estimated to be 51.79±0.47mg/100 ml and 40.57±0.75 mg/100ml respectively. The absorbance value of the fresh spermatozoa in Sperm Mitochnodrial Activity Index (SMAI) assessment by Nitro Blue Tetrazolium (NBT) assay was estimated to be 0.33±0.012. The optimum sperm: egg ratio was estimated to be 3.96 ±0.12 x 104+ no./egg. Hatching percentage of 45.97±1.72 was recorded for the cryopreserved-thawed spermatozoa while the fresh milt control recorded a hatching percentage of 73.10±0.82.
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