Antiviral activity of ivermectin was examined against R2B strain of Newcastle disease virus in 9 - day old embryonated chicken eggs. Five distinct concentrations (500, 250, 100, 50, 10 µg/mL) of ivermectin were used in the study. Haemagglutination test was performed to determine the antiviral activity of ivermectin by using chicken RBC. The results revealed that ivermectin can exert a significant antiviral activity against Newcastle disease virus at higher concentrations (500, 250 and 100 µg/ml) but with cytotoxic effects. On the contrary a moderate to weak antiviral activity without cytotoxicity was demonstrated at lower concentrations (50, 10 µg/ml).

A pluriparous non-descript doe was presented with the history of dystocia. Clinical investigation revealed the presence of closed cervix and lodged multiple foetuses in the tract. Laparo-hysterotomy was performed and four foetuses were relieved, of which one was alive, one was still born and the other two were mummified. Thus the present report describes the incidence of quadruplets in a doe comprising of dual mummies along with a stillborn and a live kid.

A pug dog was presented to Small Animal Outpatient Unit of Madras Veterinary College with the history of oral administration of 2.5 tablets of 500 mg acetaminophen. Physical examination of animal revealed a dull and depressed with pale mucous membrane. Haematological values were within normal range and biochemistry report revealed elevated Alanine amino transferase enzyme which indicated the hepatotoxic adverse effect of acetaminophen. Normal saline was administered intravenously. N-acetyl Cystine was administered @140 mg/kg intravenously after diluting in 5% dextrose. The owner was advised to give oral N-Acetyl cystine tablets at a dose rate of 70 mg/kg after 6 hrs and to repeat as such seven times. Oral liver supplements containing S-Adenosyl methionine and silybin was prescribed and advised to give at the dose rate of 5 ml/10 kg body weight. Hematinics was prescribed and advised to give orally at a dose rate of 2.5 ml/5 kg body weight twice daily.

A five-year-old intact male non-descript dog was referred to the Madras Veterinary College Teaching Hospital with a history of blindness, persistent fever, inappetence, lethargy and corneal opacity for a week. Clinical examination revealed pale mucous membrane, pyrexia, and enlarged superficial lymph nodes and ophthalmic examination confirmed bilateral corneal opacity (5/5) and related vision loss. Hemato-biochemical profile revealed anemia, thrombocytopenia, hypoproteinemia, hypoalbuminemia and elevated creatinine levels. The wet film examination was positive for Trypanosoma evansi (+++) and following which the animal was treated with Diminazene aceturate @ 3.5 mg/kg deep IM, tab. Prednisolone @0.5 mg/kg, oral hematinics and evaluated after 2 weeks. Corneal opacity cleared After 14 days of treatment; haemato-biochemical parameters were back to normal range, and after one week of clinical recovery, the dog showed neurological signs. Abdominal ultrasonography revealed mild splenomegaly, ocular ultrasonography revealed normal ocular structure. CSF was positive by PCR for Trypanosoma evansi upon which the animal succumbed to death.

In the present study, red acrylic delta traps were fabricated and evaluated in piggery and cattle farms to trap house flies. Two different pellet baits; one containing (Z)-9-Tricosene, fish meal and molasses (FMP) and other with added antioxidant, butylated hydroxy toluene (FMPB) were used in the traps. In piggery and cattle farms, the traps were suspended at a height of six feet from ground level using metal wires at a distance of three meters between each trap. Each trial was conducted for seven days and the trial was repeated six times continuously without replacement of baits. Trapped flies were counted after each trial. In piggery farm, a total of 1232 flies were trapped, out of which 454 and 778 flies were trapped in FMP and FMPB pellet baited traps, respectively. Total number of flies trapped in the first, second, third, fourth, fifth and sixth week of the study period was 308, 247, 196, 133, 204 and 144, respectively. In cattle farm, a total of 435 flies were trapped, out of which 160 flies were trapped in FMP pellet baited traps and remaining 63.21 per cent (275 flies) were trapped in FMPB pellet baited traps. Total number of flies trapped in the first, second, third, fourth, fifth and sixth week of the study period was 134, 96, 82, 55, 27 and 41, respectively. The variation in trap catches of flies in traps baited with FMP and FMPB pellets was highly significant in both piggery (χ2 value = 170.42** p<0.01) and cattle farms (χ2 value 60.80** p<0.01). Delta traps were found effective in luring house flies and can be used in livestock farms to control house flies. 

Buffaloes have high productive potential but poor manifestations of oestrus signs and silent oestrus act as a major constraint in buffaloes affecting their reproduction. There are several detection tools used for detection and confirmation of oestrus in cattle but none of the methods have succeeded to overcome the problem of silent estrus in buffaloes. In recent years proteomics approach has gained much attention to identify proteins associated with various physiological and disease conditions in saliva and cervico vaginal fluid mostly in humans and up to certain extent in cattle and buffaloes. In this study twelve pluriparous Murrah buffaloes aged 4 to 7 years with good body condition were selected and divided into group I (regular oestrus), group II (silent oestrus). Each group carries six animals and saliva was collected at proestrus (day -3), oestrus (day 0) and diestrus stage (day 7). Cervico vaginal fluid (CVF) was collected only at oestrum stage. Protein profiling of saliva were studied by SDS - PAGE analysis. The SDS-PAGE analysis showed proteins of molecular weight 150, 110, 80, 40 kDa during proestrus; 150, 120, 70 and 50 kDa during oestrus; 250, 150, 90, 50 and 37 kDa during diestrus in group I buffaloes. Similarly, proteins of molecular weight 250, 150, 100, 80 and 25 kDa during proestrus; 150, 100, 75 and 50 kDa during oestrus and 250, 100,75 and 25 kDa during diestrus were observed  in group II buffaloes. The SDS-PAGE analysis of CVF showed proteins of molecular weight of 100 and 75 kDa in group I buffaloes and 250 and 150 kDa in group II buffaloes. Oestrus specific proteins with different molecular weights were identified in terms of different bands size. However further study is warranted to find out their role during oestrus in regular and silent oestrus buffaloes for the development of specific diagnostic assay.

Lice infestation is one of the major problems to the duck farming, causing irritation that lead to lowered feed intake, retarded growth, reduced weight gain and decreased egg production. The present communication deals with occurrence of different species of lice in a desi duck from Cauvery delta region of Tamil Nadu. A farmer from Thanjavur district of Tamil Nadu brought five dead two year old native ducks for necropsy at Dept. of Veterinary Pathology, Veterinary College and research Institute, Orathanadu, Tamil Nadu. External examination of ducks revealed presence of 13 lice specimen in one bird. They were examined and identified as Trinoton querquedulae (n= 8), Anatoecus dentatus (n= 4) and Anaticola crassicornis (n= 1) morphologically. This study is the first to establish the presence of mixed lice infestation in native ducks from Cauvery delta region of Tamil Nadu and also first repot of Trinoton querquedulae in ducks from India.

A four-month-old male Labrador retriever puppy was presented to Referral Veterinary Polyclinic, Indian Veterinary Research Institute with the history of anorexia, vomiting, haemorrhagic diarrhoea and signs of hypovolaemic shock. The case was diagnosed as parvovirus gastroenteritis by rapid antigen detection test. Shock was managed by infusion of polyionic isotonic fluid.  Haematological examination revealed severe leukopenia with WBC count of 0.3×10³/µL, which was treated with administration of recombinant human granulocyte colony stimulating factor (Filgrastim) at 5 mcg/kg subcutaneously along with supportive therapy. Dog showed elevated WBC count and improvement in clinical signs after 48 h. Administration of granulocyte colony stimulating factor along with routine therapy enhanced the survivability in canine parvovirus gastroenteritis.

A 2  month old male German shepherd dog was presented to Veterinary Clinical Complex, Small Animal Surgery Unit of Madras Veterinary College, Chennai with the history of persistent regurgitation for 15 days. Plain and positive contrast radiographic evaluation revealed the dilated oesophagus cranial to the heart and tracheal deviation with constriction at the base of the heart. Surgical correction through resection of persistent ligamentum arteriosum around the oesophagus under general anaesthetic procedure. Post operative plain and contrast radiographic procedure revealed no evidence of barium stasis in the course of oesophagus and complete resolution of regurgitation. Recovery was good and normal feeding habit noticed 14th post operative day.

The current study was designed to evaluate the influence of three different ground vegetable oil seeds on various physico-chemical, microbiological and sensory attributes of chicken sausages from spent broiler breeder hen stored under frozen (-18±1°C) condition. Chicken sausages were incorporated with ground poppy, sesame and peanut seeds each at 10 % level separately as three treatments and were analyzed for their keeping quality along with high fat control. The mean values of FFA, TBARS, tyrosine values, standard plate count, psychrophilic count and yeast and moulds counts were significantly (P<0.05) influenced by formulation during frozen storage and values increased significantly (P<0.05) throughout the storage period in all chicken sausages. Chicken sausages with ground sesame scored lowest standard plate counts (5.66 log CFU/g) than any others after 180 days of storage at 18±1°C. Coli forms, lacto bacillus and anaerobic counts could not be detected during entire storage period. Scores for all sensory attributes decreased significantly (P<0.05) during storage in all treatments irrespective of formulation. Study proved that, the three ground vegetable oil seeds were effective by delaying the lipid oxidation, microbial growth and preserving sensory quality during frozen storage and among them ground sesame seed was more potent to be a better preservative.