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ACUTE TOXICITY STUDY OF NEW DAPSONE SCHIFF BASE DERIVATIVE IN LABORATORY RATS 全文
2014
Wasfi A. Al-masoudi | Hassan T. hamed | Jalaa Al- ahamed
Dapsone (4,4-diaminodiphenylsulphon) is one of the types of sulfa drugs as an anti bacterial and is used particularly in the treatment of leprosy . The aim of present study to investigate in vivo toxic effect and find acute toxic doses ( LD50) of new dapsone Schiff base derivative compound ( 4-{[ 4(ethylideneamino)phenyl] sulfonyl} aniline, was evaluated in this study. The experiment was performed on 10-14-week old male and female albino rats ( 225± 25 g ) at the time of treatment. Its acute toxic dose being 0.58 g.
显示更多 [+] 显示较少 [-]ISOLATION AND IDENTIFICATION OF SALMONELLA SPP. FROM POULTRY FARMS BY USING DIFFERENT TECHNIQUES AND EVALUATION OF THEIR ANTIMICROBIAL SUSCEPTIBILITIES 全文
2014
Ali A. AL-Iedani | Nael M. Oufi | Mohammed H. Khudor
This study aimed to isolate and identify Salmonella spp. from various sources of poultry farms by using four different techniques (conventional biochemical tests, API 20E system, serology and polymerase chain reaction) the total number of isolates was 44(9%). The Salmonellae including 4 species, S. gallinarum 9(1.85%), S. typhimurium 7(1.44%), S. newport 21(4.3%) and S. ohio 1(0.21%). The highest isolation rate was in first week of chicks life 18(25.7%), however, the highest isolation rate of salmonella was from liver 13(28.8%). There are similarity in identification rate of Salmonella spp. between API 20 E system and PCR assay using flic gene. In this study using PCR amplification of rfbsg and rfbsp genes in differentiation of Salmonella serovar gallinarum into S. gallinarum and S. pullorum biovars very useful. Results of antimicrobial susceptibility revealed high resistance of isolates against seven antibiotics arranged in descending from high to low resistance (Azithromycin, Florfenicol, Trimethoprime-sulphamethaxezole, Tetracycline, Ciprofloxacin, Ampicillin and Gentamycin).
显示更多 [+] 显示较少 [-]THE EFFECT OF GENISTEIN ON SOME PRODUCTIVE AND BIOCHEMICAL BLOOD TRAITS OF QUAIL ( Coturnix coturnix japonica ) 全文
2014
Majed H. AL-Asadi | Waleed Y. Kasim | Sajida A. Alshaheen
This study was conducted at commercial poultry farm in Aboalkassib from 9/3/2013 to 9/5/2013. A total of 60 day old quail chicks were randomly distributed to four groups , 15 birds for each treatments. Treatments were (1): control group , (2) : 100 ml genistein per 1 litter of drinking water, (3): 100 ml genistein plus 10 ml vitamin E per 1 litter of drinking water , and (4): 150 ml genistein plus 10 ml vitamin E per 1 litter of drinking water . T3 caused significantly (P
显示更多 [+] 显示较少 [-]IMMUNOMODULATORY EFFECT OF CURCUMA LONGA IN MICE 全文
2014
Abeer L. Mohammed
This study was designed to find out the effect of oral inoculation of aqueous extract of Curcuma Longa at two doses (1 and 5) mg/ kg body weight daily for 4 weeks on the immune response of Balb/c mice by estimating of serum concentration of interleukin-2 (IL-2) , interleukin-4 (IL-4), interleukin-10 (IL-10) and interferon gamma (INF-γ) using ELISA test. The present results revealed that significant increase (p
显示更多 [+] 显示较少 [-]THE LAST POSITION OF OLD WORLD SCREW WORM IN IRAQ 全文
2014
Muntasir Al-Ani | Khawla bedan Al-Jassim | Mushtaq Al-Helfi
Fourteen years of study from September 1996 to September 2010 included all province of Iraq. This study showed behavior and outbreak of old world screw worm from first recoded in Iraq with signal to its epidemiology. Investigation show proliferation and spread of fly in areas and the ways of reach this fly to in. many cases recorded at 60c and zeroC.erradication study where occur by using new method of spraying chemical pesticides and used ivermectin as injection s/c for animals as prevention. This technique improved that no cases were recorded in all town of Iraq especially in Basra which have big problem of infestation of old world screw worm in animals at 2004-2005. Study improve that were good professional staff of veterinarian in Iraq can eradication old world screw worm without using of sterile male fly technique of chrysomya bezziana.
显示更多 [+] 显示较少 [-]SYNTHESIS, CHARECTERIZATION AND ANTIOXIDANT ACTIVITY OF BIS [4-(4'-HYDROXY-3'-METHOXY BENZYLIDENE AMINO PHENYL)] TELLURIDE 全文
2014
Hiathem J. Kadhum | Mohammed A. Al-Dewan | Shaker A. S. N. AL-Jadaan
Bis[4-(4'-hydroxy-3'-methoxy benzylidene amino phenyl)] telluride (R2Te) was prepared from the reaction of two moles of 4-(4'-hydroxy-5'-methoxybenzylidene amino phenyl) mercuric chloride and one mole of TeBr 4 in dry dioxane to give diorganyl tellurium dibromide (R 2 TeBr2 ) which reduced by boiling ethanolic hydrazine hydrate to give the corresponding new telluride (R 2 Te). R2 Te inhibits nitrite induced methemoglobin formation in hemolysate. The time required to convert 50% of the available hemoglobin to methemoglobin was increased from 2.5 to 4, 5.5 and 10.5 minutes by the addition of (1.25, 5 and 20µM) of test compound (R 2 Te) respectively, and from 7 to 15, 29 and 54 minutes to convert all of the available hemoglobin to methemoglobin. It seems that R 2 Te failed to inhibit nitrite induced hemoglobin oxidation if added after the autocatalytic stage (5-10 minutes after nitrite addition). R 2 Te, a novel organotellurium compound, can inhibit nitrite induced methemoglobin formation in hemolysate in a dose dependent manner if added before the autocatalytic stage.
显示更多 [+] 显示较少 [-]THE PRODUCTION OF LACTIC ACID BY THE LOCAL IRAQI STRAIN ENTEROCOCCUS FAECIUM 全文
2014
Amal Kadhim Al Asady | Wissal Audah Hassan Alhilfi | Maytham A. Alhamdani
Lactic acid is an important carboxylic acid used in different aspects of life. In this study a pure isolate of homofermentative lactic acid bacteria Enterococcus faecium was isolated , and characterized by morphological examinations with physiological and biochemical tests. The present results have revealed the ability of the isolate to produce L- lactic acid in high concentration and low concentration of D- lactic acid in contrast with control. Lactic acid has been extracted and partially purified by Amberlite IRA400 resin column, the obtained extracted parts have been tested by carboxylic acid test in addition to High Performance Liquid Chromatography (HPLC) analysis which has showed that the concentration of L-lactic acid was 0.659g/l, while D-lactic acid was 0.174g/l in crude parts with two peaks appeared, one of area 322854 for L while no visible peak appeared for D because of small area 72214 in the figure, whereas the second peak of area 203238 may returned to the interference of D and L-lactic acid . Purified parts have reached to a purity of 72.3% for L-lactic and 38.2% for D-lactic acid also with one sharp peak for L with area of 43639 and no visible peak for D-lactic due to small area of 16713.
显示更多 [+] 显示较少 [-]BORAGE OIL RICH IN GAMMA LINOLENIC ACID (GLA) REDUCES CARDIOVASCULAR DISEASE (CVD) RISK FACTORS IN HAMSTERS FED IN DIET RICH IN SATURATED FATTY ACIDS (SFAS) AND CHOLESTEROL. 全文
2014
Ahmad Mufeed Supuh | Najeh Hapra | Mohammad Khalil Alhilal
The experiment was applied on /45/ healthy male of golden Syrian hamsters, were divided into three groups as follows: Control group(C), High Fat-High Cholesterol group(HF-HC) and Borage Oil group (BO). All groups had laboratorial diet freely during/15/ days(first period), after that each of HF-HC and BO groups took diet consisting of 80% laboratorial diet+ 20% fats(13.5% sheep fat+6.5% coconut oil) for /4/ weeks(second period). The treatment of BO group changed after second period and hamsters had laboratorial diet and dosed borage oil 2g/kg of the body weight daily for /4/ weeks(third period). The results showed a significant increase(p=0.0000) of the total cholesterol(TC), low density lipoprotein cholesterol (LDL-C) and triglycerides(TG) concentrations approximately(163%, 224%, 232%) respectively in each of HF-HC and BO groups in the second period in comparison with first period. Our results showed, in third period, a dramatic significant decrease(P=0.0000) in the TC, LDL-C and TG concentrations approximately (56%, 69%, 40%) respectively in BO group in comparison with the same group of second period
显示更多 [+] 显示较少 [-]USING TWO DIFFERENT PROMOTERS OF LYSOSTAPHIN GENE TO CHANGE PROTEIN PRODUCTION 全文
2014
Jalal Y. Mustafa
Bacteriocins are bacterial antimicrobial peptides with bactericidal activity against other bacteria. Staphylococcins are bacteriocins produced by staphylococci, which are gram-positive bacteria with medical and veterinary importance. In this study we are using two promoters from two plasmids to lysostaphin gene to change production of lysostaphin protein then killed Staphylococcus aureus and compared with pBluescript plasmid as control, pTrc99a get increased the production of lysostaphin protein while the pBAD30 are decreased the production in compared with pBluescript plasmid as control, at the same time both plasmid reported succeeded to lysostaphin expression and gave the protein with different concentration.
显示更多 [+] 显示较少 [-]ANATOMICAL STUDY OF THE PRIMARY BRONCHI AND THE LUNG OF THE BEE-EATER BIRD ( MEROPS ORIENTALIS) 全文
2014
Nabeel Abd Murad Al-Mamoori
The present study has been out to examine macroscopic structure of the primary bronchi and lung in Bee-eaters bird ( Merops orientalis) observed exist within the rib cage, the distal part of the trachea are divided into two primary bronchi (left and right) and the macroscopic appearance of the primary bronchi consists of a short tube extend caudally from syrinx to enter the proximal third of the visceral surface of the lungs through the hilus. The basic unit consisting of the primary bronchi are cartilaginous rings which takes - C - shape. The mean total length of left and right primary bronchi are (1.025 ± 0.15 cm) and (1.075 ± 0.14 cm); and the number of cartilaginous rings in left and right primary bronchi are ( 18.5 ± 0.50) and (18.5 ± 1.50). The lungs are small, pyramidal-shaped, unlobed, bright pink color, and surrounded by thin colorless membrane the pleura and the air sacs. The lung contains two surfaces (dorsal and ventral), two borders ( medial and lateral) and two extremities ( proximal and distal ). The mean total length, width and thickness of the right lung are (1.77 ± 0.17 cm), (0.95 ± 0.15 cm) and (0.4 ± 0.10 cm) while the mean total length, width and thickness of the left lung are (1.6 ± 0.15 cm ), ( 0.9 ± 0.14 cm ) and (0.37 ± 0.02 cm ).
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