Accurate detection of oestrus is a key factor for breeding and successful conception in buffaloes which is a major constraint in buffaloes. Protein concentrations of saliva and cervico vaginal fluid (CVF) were determined during various stages in normal cycling, silent oestrus and anoestrus buffaloes. Eighteen pluriparous buffaloes were selected and divided into Group I (regular oestrus; n=6), Group II (silent oestrus; n=6) and Group III (anoestrus n=6) buffaloes. Saliva was collected at proestrus, oestrus and diestrus stages of Group 1 and II animals and on a random day in Group III animals. CVF were collected only at oestrus stage of Group I and Group II buffaloes. Intense, intermediate and no oestrus signs were noticed in Group I, II and III buffaloes respectively. Non-significantly higher protein concentration was recorded in saliva and CVF during oestrus stage in Group I and II buffaloes.

An one and half years old tom cat weighing 5.2 kg was presented at Veterinary Clinical Complex, Navania, with the history of swallowing a sewing-needle along with the thread. Lateral radiograph was taken for confirmatory diagnosis. Diagnosis and a successful surgical retrieval of ingested 3.4 cms long sewing-needle attached with 17 cms long thread was carried out and the animal recovered by a week after surgery without any complications.

The effect of GnRH pretreatment on superovulatory response (SOR) and embryo yield in crossbred cattle was studied. Six crossbred cows were subjected to three superovulatory treatments: i. Control: Four day FSH schedule was initiated on Day 10 of the cycle ii. Gn-D8: GnRH was administered on Day 6 and FSH schedule was initiated on Day 8 and iii. Gn­D10: GnRH was administered on Day 6 and FSH schedule was initiated on Day 10. In control group, the mean SOR was 13.7 + 5.8 CL. In Gn-D8 group, the SOR is inconsistent (5.0 ± 1.7) with recovery of poor quality embryos, which was attributed to significantly higher progesterone concentration on the day of superovulatory oestrus. In Gn-D10 group, SOR (11.0 ± 1.6) and embryo yield were comparable to the control group. Transferable quality embryos were signcantly higher in Gn-D10 group which could be attributed to the follicular maturation under favourable endocrine environment. Thus, GnRH pretreatment in superovulation protocol ensured consistent SOR and increased yield of transferable quality embryos in crossbred cattle.

The objective of the study is to analyze the alterations in the protein profile of follicular cells in normal cyclic and acyclic anoestrus water buffaloes. Ovaries from sexually mature buffaloes were collected from abattoir and categorized into two groups viz., i. Cyclic and ii. Acyclic. In both the categories of ovaries, the follicular fluid (FF) was aspirated from all large follicles (> 9mm diameter) and small follicles (< 9 mm diameter) separately. FF was centrifuged and four categories of pelleted granulosa cells (CSG and ASG: Granulosa cells of small follicles in cyclic and acyclic groups respectively; CLG and ALG: Granulosa cells of large follicles in cyclic and acyclic groups respectively) were subjected for SDS-PAGE analysis. A total of 30 and 18 bands (from 7.1 to 209.0 kDa) were observed in CSG and CLG categories, while 16 bands in each of the ASG and ALG were recorded. It was observed that 40 per cent of bands present in the CSG group were not observed in CLG group. On the contrary, almost all the proteins found in ASG group were retained in the ALG group. It can be inferred that a group of small molecular weight granulosa cell proteins, correlating to IGF / IGFBP system, play a key role in providing a favorable proteo-genomic environment in the early stages of follicular development. In acyclic animals, retaining of such proteins in the large follicles indicated that the non-availability of metabolic factors needed for final maturation of follicles.

A 2 years old Labrador dog brought with history of ingestion of a newly paper covered packet shaving blade immediately falling from owner's hand during his shaving one day after the incident. There were no clinical signs observed and the dog was normal. Lateral radiography taken showed radio-opaque shaving blade in the stomach area. Solid food was not given after ingestion of shaving blade till surgical intervention and under general anaesthesia shaving blade was removed surgically. The stomach was sutured with vicryl no. 1/0 in continuous lumbert and cushing pattern in double layer. Post-operatively administration of antibiotics, analgesics and regular dressing of wound was done and dog recovered uneventfully.

Bovine Lymphocyte Antigen DRB3 (BoLA-DRB3), a gene of the major histocompatibility complex (MHC) has received attention because this is considered to be a potential genetic marker associated with disease resistance traits in cattle. Hence, a study was taken up to characterize BoLA-DRB3.2 alleles in the 60 Ongole animals. The most common alleles observed in Ongole cattle was *15 and *6 with frequency of 0.225 and 0.200, respectively. Of the 22 identified alleles detedted, these 7 allleles (BoLA-DRB3.2*15,*6,*12,*13,*23, *31 and *47) represented 77.5 percent of allelic frequencies. Result indicated that the BoLADRB3 exon 2 was highly polymorphic in Ongole cattle. Direct sequencing BoLA-DRB3.2 allele reveled that at nucleotide at position 91 and 133 of the sequence had both A and G nucleotides in contrast to T nucleotide of Bos taurus breed and the variations leads to different band pattern in gels.

A study was conducted to assess the quality control practices of both physical and chemical methods of evaluation adopted by feed mills in India while procuring the feed ingredients for preparing poultry feeds. Based on physical methods of evaluation of different feed ingredients, 6.4 % of lots were found to be inferior quality out of 2606 lots tested. The results indicated that the moisture content of grains procured during the period from October to December months was high whereas it was low from April to July. The protein content of soybean meal varied from 45.5 to 47.5% while its solubility varied from 72.1 to 83.2%. The rejection of raw material consignments was 7.85 %. The major reasons for rejection included high moisture, low protein, high level of sand amp; silica, adulteration / contamination with foreign materials. the findings of this study emphasis ther sgnificance of strict quality control measures to be adopted to ensure feed and food safety.

A survey was conducted to assess the breeder production performance in Aseel breeding farms. Aseel birds had multicolored plumage with solid feather patterns distributed all over the body. Pea combs, strawberry and single comb were observed in the population. Birds were housed in layer shed with the ratio 1 male for 8 female. Age at first egg was  recorded as 139±1.94 days. Average cumulative egg production up to 120 weeks per bird was 160.59±0.59 with the 77% hatchability. Percentage hen day egg production for Aseel was 29.72±1.60 and the effective population size (Ne) ranged from 352.94 to 4781.16 and the overall mean Ne in the Aseel population was 2086.59±148.35 with the rate of inbreeding 0.0003.

A retrospective study was carried out to assess the udder affections of cows that were presented to the Large Animal Medicine Referral Clinic of Veterinary College and Research Institute, Thanjavur, Orathanadu, Tamil Nadu during June 2017 to April 2018. In this study involving 161 cows, 4 cows were found to have udder swelling / abscess diagnosed by ultrasound and percutaneous aspiration done under sonagraphic guidance. Udder ultrasound revealed hyperechoic alveolar tissue with anechoic/ heterogenous content in the affected quarters. Aspirated samples were culturally positive for the isolate Staphylococcus aureus and E. coli. In vitro antibiotic sensitivity test showed highest susceptibility to streptomycin followed by enrofloxacin and ciprofloxacin, gentamicin and amoxycillin with good recovery on treatment.

Trazodone is a commonly used medication in the United States for the treatment of anxiety in dogs.This article is intended to bring awareness among small animal veterinary practitioners of India about trazodone and its potential uses in the treatment of post-operative hyperactivity, noise phobia, separation anxiety and aggressive behavior.