The bacteriocin nisin at various concentrations (50, 100, 200, 300 IU/ml) was studied as a bio-preservative to extend the shelf life of standardized, pasteurized milk. A minimum of one-week extension in shelf life was obtained for standardized milk at 4°C. Higher concentrations of nisin showed no additional effect. The effect of nisin on physico-chemical, microbial and sensory qualities were also studied. Addition of nisin had significantly reduced the development of acidity and standard plate count during storage. Addition of higher levels of nisin had significantly affected the sensory scores.

The data on 1721 calves from Sahiwal and Jersey-Sahiwal crossbred cattle were utilized for studying the age specific mortality and culling rate. The overall mortality and culling rate were found to be 15.92 and 19.52 per cent in Sahiwal and Jersey crossbreds respectively. The average female calf mortality from 0-1, 1-3, 3-6, 6-12 and 12 month to age at first calving was observed as 5.81, 2.92, 2.06, 1.75 and 5.01 per cent, respectively. The genetic group had no significant effect on female calves mortality. The maximum culling rate was observed from 12 month to AFC.

With the aim to examine the effect of rearing systems on major meat quality traits in native ducks, appropriate experimental investigation was conducted. Research material included a total of 160 day-old native ducklings. The experiment was organized in two treatments with two replications each. Two different rearing systems were employed the intensive (cages) and extensive system (free-range). The native ducklings belonging to same hatch were procured from a local duck farmer and reared simultaneously for a period of ten weeks. Twelve ducks (six male and six female) from each treatment were chosen randomly and slaughtered at the end of rearing period to study meat quality traits in native ducks. Differences were significant (P<0.05) among sexes and rearing systems on pre slaughter live weight, eviscerated weight and various cut up parts, the corresponding values are higher for intensively reared ducks. Regarding sex, both male and female ducks exhibited differences considerably higher weight gain, carcass and cut up parts yield than that of female ducks.

Six dogs with unstable diaphyseal fracture of femur of different breeds of either sex and bodyweight ranging from 10 to 30 kg presented to the Small Animal Orthopedic Unit of the Madras Veterinary College Teaching Hospital, over a period of two years underwent fracture fixation with latest technique of linear locking compression plates. Different sizes and length of new generation locking plate system were used for different configuration of unstable fracture of femur. The post operative assessment of fracture healing was evaluated by radiographs at different intervals and estimation of bone specific serum alkaline phosphatase enzyme by fluorimetric methods. In the present study, the locking compression plating technique provided adequate apposition, stable fixation and promoted early weight bearing of traumatized limb. The locking plate system had a unique combi hole design in a single implant and enabled to select the function best suited for the fracture configuration to achieve the most stable fixation. The locking plate system acted as a single beam construct which increased the stiffness of the implant and was found appropriate in management of unstable or comminuted diaphyseal fracture of femur in dogs.

A clinical parameter study was conducted to file the reference serum bio-chemical values of Bonnet macaques (Macaca radiata) reared under captivity. The animals were chemically restrained and six blood samples (3 males and 3 females) were collected from the saphenous vein and processed. In this study, the mean ± S.E. of estimated value of serum biochemical profiles were close to the normal range reported earlier. But, serum glucose level was less and serum level of phosphorus was comparatively high.

Methane (CH4) is second major gas after carbon dioxide (CO2) responsible for the warming of environment and ozone layer depletion. Although CH4 production is necessary for efficient digestion, it represents an energetic loss of up to 12% of the gross energy intake in ruminants. Methane is produced by strict anaerobes belonging to the sub-group of the Archaea domain. The past decade has shown a lot of interest in the use of plant extracts to mitigate methane production in ruminants. Addition of plant essential oils, can limit the growth of the micro-organisms participating in methane formation in the rumen, thus resulting in the reduction of its production. Reduction of methane production in the rumen ecosystem is possible to achieve with the use of plantsaponins. Tannins have been found to be toxic for many of the rumen microbes, especially ciliate protozoa, fibre degrading microbes and methanogenic bacteria as a result of this methanogenesis in the rumen is reduced. 

Meat tenderization is the disruption of meat structure, breaking the collagen subsequently resulting in good palatability and acceptance by consumer. Tenderization could be achieved by the traditional method of ripening for a long period in controlled temperature with due precautions to prevent meat spoilage. In this process endogenous muscle enzymes viz. calpains, cathepsins and caspases are responsible for proteolysis of muscle. Other processes adopted for tenderization include use of electricity, heat, physical force (hydrostatic pressure), ultrasonic waves, shock waves in water (hydrodyne), enzyme action, use of vitamins, ionic compounds, mineral salts, and chemical compounds. The electrical stimulus of low voltage is more popular than high voltage due to the cost involved. Like endogenous enzymes, plant based exogenous enzymes also cause tenderization, but their activity should be monitored to avoid over-tenderization. Chemicals, vitamins and ionic compounds activate the calcium-dependent proteases and lysosomal enzymes, which are responsible for tenderization. 

An adult Nellore brown sheep was presented to Veterinary hospital with a history of swollen unilateral protrusion of left eyeball with congestion of conjunctival mucous membrane, exophthalmos condition since 5-6weeks. Clinical examination revealed blindness due to chronic keratitis and on palpation found a large fluid filled cyst in the left eye. With the surgical intervention the cyst was removed successfully along with its membrane from the retrobulbar region of the left eye and on parasitological examination it was diagnosed as Coenurus gaigeri cyst. Surgical intervention-involved treatment coupled with antibiotic therapy was proven to be an efficient way of managing retrobulbar coenurus cases.

With the advancement of research and development of medical technology, there is an increase in the number of animals used in research. As millions of experimental animals are being used in different experiments worldwide, there is a lot of debate about the pain, distress, and death experienced by animals during scientific experiments. Experimental animals also require skilled manpower, time-consuming protocols and are costly. There are various alternatives to animal testing to overcome the drawbacks of animal experiments and avoid unethical procedures. Russell and Burch published principles of the Humane Experimental Technique which includes a strategy of 3 Rs (i.e. reduction, refinement, and replacement) in 1959. They introduced and defined the terms replacement, reduction, and refinement, which subsequently have become known as ‘alternatives’ or ‘alternative methods’ for minimizing the potential for animal pain and distress in biomedical research. Therefore, different methods and different alternative organisms are being used to implement the 3 Rs strategy. So, a brief account of these alternatives and the advantages associated is discussed in this review with examples. An integrated application of these approaches would give insight into the minimum use of animals in scientific experiments.

An experiment was conducted to study the effect of sex on growth pattern and carcass characters of commercial broilers. One hundred and twenty numbers of day-old chicks were wing banded, weighed and reared up to 40 days of age. The body weight was individually recorded from all the birds on 7, 14, 21, 28, 35 and 40 days of age to assess the influence of gender on body weight. At the end of the experiment 12 males and 12 females were subjected to slaughter studies to assess the carcass characters. The body weight between males and females were similar up to 14 days of age and thereafter, males recorded significantly (p<0.01) higher body weights on 21, 28, 35, 40 days of age, weighing 35.79, 107.69, 179.73, 211.88 g respectively higher than females. The ready-to-cook weight (%) and dressed carcass (%) were significantly (p<0.05) higher in males; whereas, New York carcass (%) was significantly (p<0.05) higher in females. Among the cut of parts, the drumstick percentage was significantly (p<0.05) higher in males, whereas, the wings percentage was significantly (p<0.05) higher in females. Among processing yields and losses, only shank & feet and abdominal fat (%) showed significant (p<0.05) difference between sexes; while, feather, blood, head, viscera, kidney and total offal yields had non-significant difference between sexes. Among giblets, only the heart yield was significantly (p<0.05) different between sexes with higher value in females. The results showed that the males attain marketable weight early, therefore can be marketed early. The males can be slaughtered earlier than females for producing specialized products from drumstick; whereas, females can be slaughtered early to harvest drumette.