Isolation and identification of bacteria from haemolymph, stomach and hepatopancreas (HP) were carried out from 37 farms in Tamil Nadu, India to find out the prevalence of Vibrio parahaemolyticus (Vp) infections with special reference to acute hepatopancreatic necrosis disease (AHPND). Bacterial identifications were done based on the morphological, physiological and biochemical characteristics. Vp isolates were identified by polymerase chain reaction (PCR) targeting Vp specific toxR and tlh genes, human pathogenic tdh and trh genes, and AHPND causing AP1, AP2, AP3 (pirAvp) and AP4 (pirAvp and pirBvp) genes. Histopathological and immunohistochemical (IHC) techniques were employed to identify the pathology due to Vp in HP tissues. Based on the morphological, physiological, biochemical and molecular characterization, 74 isolates were identified as Vp (35.14%), V. harveyi (21.62%), V. anguillarum (16.22%), V. campbellii (10.81%), V. mimicus (8.11%), V. alginolyticus (5.41%), and V. aeruginosa (2.7%). All isolates were negative for tdh, trh, AP1, AP2, AP3 and AP4 genes. Histopathology and IHC revealed that the HP was the prime organ affected in Vp infection. It further proved that the IHC could be used as an important tool to identify the establishment of infection in hepatopancreatic tissues.

A study was conducted to assess the factors inÀuencing the occurrence of downer cow syndrome in Tamil Nadu. For this, primary data from the 67 dairy farmers (60 cow and 07 buffalo farmers) from Namakkal and Karur districts of Tamil Nadu having downer cow syndrome affected dairy animals were collected. Using logistic regression model, Wald statistic were obtained for the independent variables which indicated that the coefficients for parity, stage of lactation, summer and winter season, previous occurrence of metabolic diseases, feeding of green fodder, feeding of concentrate, proximity to parturition (near term) and species of dairy animal were observed significant. The log odds of the animal going to be affected by downer cow syndrome enhanced by 8.199 and 4.581, when parity and mid-stage of lactation were changed from 0 to 1. These findings suggested that more concentration on these factors were warranted to minimise the risk of incidence of downer cow syndrome in bovines.

Thirty healthy, parous, cyclic crossbred cows aged under 8 years were selected and randomly divided into two groups as group I (n=10) and group II (n=20). Group I served as control and group II cows were synchronized with CIDR + PGF2α protocol in the presence of matured CL. Breeding was carried during the observed estrus in group I and at 48 and 72 h after removal of CIDR in group II cows with good quality frozen thawed Jersey crossbred semen. The group II cows showed 100 per cent estrus induction with a mean time of onset of induced estrus of 46.20 ± 0.50 h in group II cows. The duration of estrus was 25.40 ± 0.45 and 26.30 ± 0.47h in group I and group II, respectively. The incidence of intense and intermediate estrus was significantly higher in group II when compared with group I cows. The length of estrus in non-conceived cows was 22. 13 ± 0.52 and 21. 37 ± 0.62 days in group I and II, respectively with a conception rate of 20 and 60 per cent in group I and II. It was concluded that application of CIDR and PGF2α with FTAI was effective in improving conception rate in cross bred cows.

On post-mortem examination of five desi chickens, cherry red coloured spindle shaped worms were collected from the proventriculus of each bird for identification. Based on the morphological examination, these worms were identified as Tetrameres mohtedai. A high prevalence of Tetrameres mohtedai helminth infection was documented in desi chickens in Cauvery delta region of Tamil Nadu, which causes high morbidity and mortality in desi chickens from Cauvery delta region of Tamil Nadu. Appropriate deworming strategies need to be followed in desi chicken for the control of parasite in this region.

Globally, many clinico-pathologic differences between hounds and other breeds have been investigated and most of the research has been focused on differences in the hematologic values between the breeds and hematologic reference intervals for the Greyhounds, which have been recently published. However, our native hound, Chippiparai, closely resembles the morphological traits of the hounds and yet there are no published reports on the hematological parameters of the Chippiparai breed from Tamil Nadu. Hence, this study was carried out to investigate the red cell variables like Hemoglobin, Packed Cell Volume and RBC counts which were compared with that of non-hound breeds. Chippiparai hound dogs (n=30) and non-hound dogs (n=30) were selected amongst the dogs presented at Madras Veterinary College Teaching Hospital and various native hamlets. The Hb, PCV and RBC level sin the Chippiparai breeds were found to be higher which may probably be attributed to the need for appropriate oxygen supplementation under extreme climatic conditions, for running and hunting and probably as an inheritance from the other sight hounds. Also, these breeds can be  recommended as good blood donors since a lesser volume of their transfused blood (with greater PCV) can increase the hematological parameters of the anemic recipient dog as compared to an equal volume of blood from the non-hound blood donors.

The house fly sex pheromone (Z)-9-tricosene was impregnated into different pheromone dispensers (septa, plastic and polythene) and their efficacy in luring Muscadomestica towards red acrylic delta trap was studied. Out of the 514 house flies trapped, (Z)-9tricosene when impregnated in circular plastic green lure attracted the maximum number of flies (165), followed by polyvial (132), silicone septa (111) and rubber septa (79) whereas the least number of flies was attracted with rectangular plastic dispenser (27). Among the house flies trapped, 46.49 per cent (239) were males and 53.50 per cent (275) were females redominance of male flies was observed with circular plastic green lure, polyvial and silicone septa, whereas in rubber septa and rectangular plastic lure, more number of female house flies were trapped. Observations revealed that circular plastic green lure, polyvial and silicone septa can be used as an effective dispensers to impregnate (Z)-9-tricosene in order of preference to facilitate slow and sustained release of pheromone and thereby trapping the optimum number of house flies towards delta trap.

An investigation was carried out to assess the nurtional , texture profile, microbial and sensory quality of wafer cones. Refined wheat flour and water, lecthin oil, vegetable fat, baking powder, SMBS (Sodium Meta Bisulphite) were used for the preparation of the wafer cone. Treatments were prepared with casein powder at the rate of 3, 5 and 7 per cent for enriching the wafer cones and control samples were prepared by using refined wheat flour. The quality assessment for moisture, protein and fat was carried out using infrared analyser from NDC technologies. Sensory acceptability was assessed by 9- Point Hedonic Scale and results were analysed statistically.

A 3 time calved cross bred Jersey cow with its 20 days old calf was presented to the outpatient medical unit of Veterinary Clinical Complex, Veterinary College and Research Institute, Namakkal with the history of anorexia, mild respiratory distress and eruptions on the skin for a period of 10 days. General clinical examination revealed high temperature, anaemia, icteric conjunctival mucous membrane, enlarged prescapular amp; cutaneous lymph nodes, eruptions on the skin and presence of ticks on the body. Fine needle aspiration cytology of pre scapular lymph node and pheripheral blood smear examintaion confirmed theileriosis. Incidentally its 20 days old calf too had similar symptoms. Both the dam and the calf were treated with suitable therapeutic regimen including blood transfusion.

Tiruchy Black is one of the coarse wool breeds of sheep reared in Tamil Nadu. An investigation was undertaken to ascertain the migratory pattern and socio-economic status of Tiruchy Black sheep at 71 flocks in 53 villages of Dharmapuri and Krishnagiri Districts of Tamil Nadu. The migratory tracts were almost regular over the year in the breeding tract. A total of eight migratory tracts comprising of three major and five minor tracts were identified. The overall distance covered was 98.64 ± 1.13 km with a range of 15 to 128 km. The mean radial migratory distance was 184.12 ± 1.42 with range from 65 to 234 in major tracts and 34.12 ± 1.62 with a range of 15 to 64 Km in minor tracts. The Tiruchy Black sheep farmers family size was comprised of 2-5 numbers and both the genders were involved in sheep rearing. In the present study, it was observed that vast majority of sheep farmers belonged to the most backward (72.16 %), followed by backward (22.37 %) and others (5.47 %). Majority of sheep farmers belonged to the ‘Kurumba Gounders’ (96.00%). A small proportion of them in small farms belonged to the Vanniyars (4.00%). Statistical analysis revealed no significant relation between caste, community and farm size. Depending upon the condition of the animal, prices of adult rams and ewes varied from Rs. 7,000 to 8,000 and from Rs.5000 to 6,000 respectively. Surplus ram lambs were sold at the age of 3 to 5 months for Rs.1,750 to 2,500. It is concluded that the Tiruchy Black sheep farmers were following traditionally migratory pattern over the generations and which helps improves their socio­economic status in the breeding tract of Tamil Nadu.

In present day, supplementation of extra minerals and vitamins is highly essential in commercial diets due to high productivity and to withstand the detrimental effects of different stresses. Selenium is one of essential trace minerals for better growth and productivity as well as anti-stressor in commercial broilers. Nano-selenium can effectively be synthesized through High Energy Ball Milling (HEBM) technique from its precursor, for use in commercial broilers as anti-stressor and to support multiple bodily functions. The prepared nano particle had 44.5 % of selenium as measured by Energy Dispersive X-ray (EDAX) analysis with the product yield of 50 g/hr. The chemical composition of sodium selenite powder was same as that of the original mega particle. The size of Se nano particle ranged from 37-85 nm as analyzed through Field Emission Scanning Electron Microscope (FESEM). X-Ray diffraction pattern confirmed that the synthesized Se nano particle was free of impurities and provided accurate information on the atomic arrangements. The Fourier Transform Infra-Red (FTIR) spectrum of synthesized nano particle source of selenium peaks was located at 3023.26, 2800.12, 2502.23, 2314.17, 1610.40 and 1413.30 cm-1 which showed chemical bonding in a target material. The zeta potential of nano selenium was -23.30 mV when analyzed through particle size analyzer. Se nano-particles could be successfully synthesized through High Energy Ball Milling method from its precursor and could be characterized for its quantity, size, shape, stability and purity. The synthesized Se nano-particles could be utilized for the conduct of biological trial in commercial broilers.