This study was carried out to investigate the resistance phenotypes and resistance genes of Escherichia coli from swine in Guizhou, China. A total of 47 E. coli strains isolated between 2013 and 2018 were tested using the Kirby-Bauer (K-B) method to verify their resistance to 19 common clinical antimicrobials. Five classes consisting of 29 resistance genes were detected using polymerase chain reaction. The status regarding extended-spectrum β-lactamase (ESBL) and the relationship between ESBL CTX-M-type β-lactamase genes and plasmid-mediated quinolone resistance (PMQR) genes were analysed. A total of 46 strains (97.9%) were found to be multidrug resistant. Amongst them, 27 strains (57.4%) were resistant to more than eight antimicrobials, and the maximum number of resistant antimicrobial agents was 16. Twenty antibiotic resistance genes were detected, including six β-lactamase genes blaTEM (74.5%), blaCTX-M-9G (29.8%), blaDHA (17.0%), blaCTX-M-1G (10.6%), blaSHV (8.5%), blaOXA (2.1%), five aminoglycoside-modifying enzyme genes aac(3′)-IV (93.6%), aadA1 (78.7%), aadA2 (76.6%), aac(3′)-II c (55.3%), aac(6′)-Ib (2.1%) and five amphenicol resistance genes floR (70.2%), cmlA (53.2%), cat2 (10.6%), cat1 (6.4%), cmlB (2.1%), three PMQR genes qnrS (55.3%), oqxA (53.2%), qepA (27.7%) and polypeptide resistance gene mcr-1 (40.4%). The detection rate of ESBL-positive strains was 80.9% (38/47) and ESBL TEM-type was the most abundant ESBLs. The percentage of the PMQR gene in blaCTX-M-positive strains was high, and the detection rate of blaCTX-M-9G was the highest in CTX-M type. It is clear that multiple drug resistant E. coli is common in healthy swine in this study. Extended-spectrum β-lactamase is very abundant in the E. coli strains isolated from swine and most of them are multiple compound genotypes.

Toxoplasma gondii, an obligate intracellular parasite, is the aetiological agent of toxoplasmosis, a disease that affects approximately 25% - 30% of the world's population. At present, no safe and effective vaccine exists for the prevention of toxoplasmosis. Current treatment options for toxoplasmosis are active only against tachyzoites and may also cause bone marrow toxicity. To contribute to the global search for novel agents for the treatment of toxoplasmosis, we herein report the in vitro activities of previously synthesised benzyltriazole derivatives. The effects of these compounds against T. gondii in vitro were evaluated by using a expressing green fluorescent protein (GFP) type I strain parasite (RH-GFP) and a type II cyst-forming strain of parasite (PruΔku80Δhxgprt). The frontline antitubercular drug isoniazid, designated as Frans J. Smit -isoniazid (FJS-INH), was also included in the screening as a preliminary test in view of future repurposing of this agent. Of the compounds screened, FJS-302, FJS-303, FJS-403 and FJS-INH demonstrated > 80% parasite growth inhibition with IC50 values of 5.6 µg/mL, 6.8 µg/µL, 7.0 µg/mL and 19.8 µg/mL, respectively. FJS-302, FJS-303 and FJS-403 inhibited parasite invasion and replication, whereas, sulphadiazine (SFZ), the positive control, was only effective against parasite replication. In addition, SFZ induced bradyzoite differentiation in vitro, whilst FJS-302, FJS-303 and FJS-403 did not increase the bradyzoite number. These results indicate that FJS-302, FJS-303 and FJS-403 have the potential to act as a viable source of antiparasitic therapeutic agents.

Objective: Newborn calf diarrhea causes significant economic losses. Therefore, this study was aimed to determine the effect of adding probiotics on Total Antioxidant (TAS) and Total Oxidant (TOS) levels in routine treatment of calf diarrhea. Material-Method: The material of the study consisted of 12 calves, routine treatment (Group 1, n=6 calves) and probiotic group. (Group 2; n=6 calves).Results: TAS values increased statistically after treatment (P <0.05) in group 2 compared to pretreatment. Comparison of the groups after treatment revealed that TAS significantly increased (P <0.05) in group 2. While there was no statistical difference in TOS values between the groups a statistically significant increase (P <0.05) was detected after treatment compared to pretreatment.Conclusion: It was concluded that adding probiotics may be useful in addition to routine treatment in newborn calf diarrhea.

To compare the effect of intraperitoneal administered Carvacrol with bevacizumab in an oxygen-induced retinopathy (OIR) model in rats. Twenty-eight newborn rats were included in the study and the OIR model was created with the 50/10% oxygen style. The study consisted of 4 groups and each rat in the groups received an intraperitoneal injection of 0.01 mL once on the postnatal 14th day. While the OIR model was not created in Group I (control group), it was created for Groups II, III, and IV. Groups I and II were injected with 0.9% NaCl solution, Group III with bevacizumab, and Group IV with carvacrol. The rats were sacrificed on the postnatal 18th day.Histopathological and immunohistochemical studies showed that the number of retinal vascular endothelial cells (RVECs) and nuclear factor (NF)-κB levels decreased similarly in Group III and Group IV compared to Group II. RVECs values for Group I, Group II, Group III and Group IV were measured as 1.26±0.80, 27.10±3.63, 7.54±1.38, and 6.22±1.22, respectively and it differed significantly between groups (p<0.001). Likewise, NF-κB levels were recorded as 0.61 ± 0.30, 4.36±0.65, 2.68±0.44, and 2.85±0.58, respectively and it differed significantly between groups (p<0.001). On the other hand, RVECs and NF-κB levels were similar between Group III, and Group IV (p values were 0.58 and 0.91, respectively).The study demonstrated that carvacrol significantly reduced retinal pathological neovascularizations, RVECs, and NF-κB levels. Moreover, the observed effects were comparable to those of bevacizumab.

This study was designed to provide important clinical signs for tracking nerves in the mandible during regional anesthesia in Gurcu goats. Materials and methods: The study was conducted on the mandible bones of ten adult Gurcu goats of both sex (five male and five female). The mandible bone samples of naturally dead Gurcu goat were collected from Kafkas University veterinary faculty education, research, and application farm and processed as per the standard maceration technique. Altogether, 16 measurements were taken in the mandible bones of Gurcu goats by using a digital caliper and the results were expressed as mean and standard deviation.The obtained parameters from the present study can be useful for an extraoral and intraoral approach for nerve block of mental and mandibular nerve in the mandibular regions of Gurcu goat. According to results the mandibular length and height were 158.86±10.37 mm, 89.38±5.81 mm, in females and 198.93±3.85 mm, 114.5±7.29 mm, in males of Gurcu goats, respectively. The distance between the first inferior incisor tooth and mental foramen and to the first premolar tooth was 19.72±2.3, 19.26±0.44 mm in females, and 29.41±6.10, 21.83±1.02 mm, in males, respectively. The present study revealed that all the obtained parameters related to regional anesthesia showed a significant statistical difference (p<0.01**) between the males and females of Gurcu goat.It can be concluded from the present study that the various applied parameters of the present study are thought to assist clinicians in the administration of regional anesthesia in the lower jaw area (mandibular region) of the Gurcu goat.

The aim of this study is to determine the incidences of Malassezia infection and flea allergy in dogs with dermatitis complaints which were brought to veterinarians for examination in Ankara and Kırıkkale provinces. Materials and Methods: The material for this study consists of 40 pet dogs of different breeds genders and ages found in Ankara and Kırıkkale provinces of Turkey. Malassezia examination was conducted by staining smear samples taken with the acetate band method with Modified Wright’s Stain method. Flea existency examination was performed by using a flea comb.Out of the 40 dogs with dermatitis which were included in this study, 6 dogs (15%) were found to have Malassezia infection, 12 dogs (30%) were found to have flea infestation and 4 dogs (10%) were found to have together.In light of the results that were found in this study, Malassezia and flea infestation hold an important place for dogs which were brought with complaints of dermatitis to veterinary clinics for examination in Ankara and Kırıkkale provinces. Clinical veterinarians must keep in mind that, there is high incidence rates of these 2 dermatitis causing agents and also remember to especially consider them in the list of differential diagnosis.

It was aimed to investigate whether chia seeds provide an advantage during ultrasonographic imaging in cats when added to food by means of its high water-holding capacity. Ten healthy cats were ultrasonographically examined following 12- hour fasting period and after feeding with 3 different food types at 1-week intervals. Thus, 4 study group were formed as follows; FST; fasting 12- hour period, WF; feeding with wet cat food following 12- hour fasting period DWF; feeding with ½ of same wet food diluted with the same volume drinking water, CWF; feeding with ½ of same wet food mixed with 36 ml swollen chia seeds obtained by holding 2 hours 3ml chia seeds in 33 ml drinking water. Cats were evaluated ultrasonographically immediately after feeding, and at the 30th minutes and 60th minutes.Especially in terms of thickness, gastric wall evaluation was easier after food intake. In the CWF, the stomach was fully imaged on the frame at all times. The gastrointestinal tract content moved faster when cats were fed DWF or CWF; In the examination immediately after feeding the contents were in duodenum and seen in the jejunum and ileum in the 30- and 60-minutes’ examinations. CWF and DWF revealed more heterogeneous images then WF and also CWF provided better visualization for the intestinal canal wall layer.Ultrasonographic examination after feeding with chia seed added formula can be used as a complementary method following the fasting examination.

Factors such as location, volume, and the type of neoplasm complicate achieving tumor control. Electrochemotherapy (ECT) is a supplementary treatment for inoperable neoplasms in veterinary patients. Three dogs were diagnosed with a tumor. Two were squamous cell carcinoma (SCC), and the other was liposarcoma, each with a single tumor with the size range of 1 to 5 cm. The tumor locations were the cervical, oral, and abdominal cavity. ECT was selected as a treatment. Bleomycin was injected intratumorally at the dose of 0.5 to 1.0 mg/cm³. Five minutes after the injection, electric pulses applied in a sequence of eight pulses lasting 100 µsec each, were delivered in 1,000 V/cm. An evaluation was performed after 1 week, and the next session was administered 2 weeks later. In a patient with oral SCC, the tumor was in partial remission after two sessions of ECT. Another patient with SCC on her neck was showed complete remission after 2 weeks of ECT administration. A third patient showed stable disease for 8 weeks. Complications were mild and transient and included skin necrosis, edema, local pain, and gait disturbance. ECT is a valid adjuvant, especially for inoperable, cutaneous, or accessible intra-abdominal tumors.

The purpose of this retrospective study was to provide additional data on the use of toceranib in a wide variety of tumor types in small breed dogs, especially < 8 kg (except 5 dogs). This was a retrospective study of 31 dogs with malignant tumors treated with a 2.5 mg/kg median dose of toceranib (Palladia; Zoetis, USA) on a Monday-Wednesday-Friday schedule. Clinical benefit was observed in 13 of 15 dogs (86.7%, 3 with complete response, 4 with partial response, 6 with stable disease) with gross disease. Distant metastasis, response to treatment, and treatment setting were significantly associated with survival time. Negative prognostic factors were multiple chemotherapy and distant metastasis (affecting progression-free survival [PFS]), surgery, regional enlarged lymph nodes, underlying disease, and toxicity (affecting median survival time [MST]). Positive prognostic factors were epithelial and round cell tumor (affecting PFS), epithelial tumor, microscopic disease, no evidence of disease response, and stable disease (MST). In conclusion, a clinical benefit from toceranib treatment was noted in most of the dogs with gross disease in our study. This study suggested that the toceranib is probably selective treatment to various tumor types in small breed dogs.

The reproductive tracts have an intimate relationship with reproduction because there are bacterial communities that can affect reproductive health. The differences in the bacterial community of periparturient dairy cows were investigated. Vaginal and fecal samples were collected seven days before and after calving, and DNA was extracted to sequence the V3-V4 regions of the 16S rRNA genes. In the postpartum vaginas, operational taxonomic units, Chao1, Shannon, and Simpson were decreased, and phyla Fusobacteria and Bacteroidetes were increased. In summary, bacterial abundance can affect the periparturient biological differences in dairy cows, suggesting a susceptibility to infection within one week after calving.