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Risk of establishment of canine leishmaniasis infection through the import of dogs into South Africa 全文
2019
Abdalla A. Latif | Bonginkosi Nkabinde | Brian Peba | Olivier Matthee | Ronel Pienaar | Antoinette Josemans | Daniel Marumo | Karien Labuschagne | Nada A. Abdelatief | Andreas Krüger | Ben J. Mans
Risk of establishment of canine leishmaniasis infection through the import of dogs into South Africa 全文
2019
Abdalla A. Latif | Bonginkosi Nkabinde | Brian Peba | Olivier Matthee | Ronel Pienaar | Antoinette Josemans | Daniel Marumo | Karien Labuschagne | Nada A. Abdelatief | Andreas Krüger | Ben J. Mans
Canine leishmaniasis is a vector-borne disease caused by protozoa of the genus Leishmania that affect dogs, humans and wildlife. Sandflies of the genera Phlebotomus and Lutzomyia are the primary vectors. Canine leishmaniasis is an exotic and controlled disease in South Africa. The main purpose of our risk assessment study was to evaluate the likelihood that this exotic disease could enter and be established in South Africa through importation of live dogs. Risk analysis to the spread of the disease follows the World Organization for Animal Health (OIE) formal method of quantitative risk assessment documented as a step-by-step process. We have identified and discussed 11 possible risk factors involved in three steps for final assessment. The annual average number of diagnostic tests performed on imported dogs from 44 countries for 2011–2015 was 1158. Leishmania is reported to occur in 21/44 (47.7%) exporting countries. A total of 71.1% of Leishmania positive dogs were imported from these endemic countries. The yearly percentage of Leishmania positive dogs ranged from 0.2% to 2%. Three confirmed clinical and fatal cases of leishmaniasis in dogs of unidentified origin have been reported by our laboratory and the state veterinarians. The disease has been reported in neighbouring countries as well as the putative sandfly vectors. This study concluded that the risk for the introduction and degree of uncertainty of Leishmania in imported dogs in South Africa are moderate. Risk mitigation and recommendations such as investigations into possible occurrence of autochthonous leishmaniasis in the country, surveillance in its wildlife reservoirs and systematic surveillance of sandfly populations are discussed.
显示更多 [+] 显示较少 [-]Risk of establishment of canine leishmaniasis infection through the import of dogs into South Africa 全文
2019
Latif, Abdalla A. | Nkabinde, Bonginkosi | Peba, Brian | Matthee, Olivier | Pienaar, Ronel | Josemans, Antoinette | Marumo, Daniel | Labuschagne, Karien | Abdelatief, Nada A. | Krüger, Andreas | Mans, Ben J. | Agricultural Research Council - South Africa
Canine leishmaniasis is a vector-borne disease caused by protozoa of the genus Leishmania that affect dogs, humans and wildlife. Sandflies of the genera Phlebotomus and Lutzomyia are the primary vectors. Canine leishmaniasis is an exotic and controlled disease in South Africa. The main purpose of our risk assessment study was to evaluate the likelihood that this exotic disease could enter and be established in South Africa through importation of live dogs. Risk analysis to the spread of the disease follows the World Organization for Animal Health (OIE) formal method of quantitative risk assessment documented as a step-by-step process. We have identified and discussed 11 possible risk factors involved in three steps for final assessment. The annual average number of diagnostic tests performed on imported dogs from 44 countries for 2011–2015 was 1158. Leishmania is reported to occur in 21/44 (47.7%) exporting countries. A total of 71.1% of Leishmania positive dogs were imported from these endemic countries. The yearly percentage of Leishmania positive dogs ranged from 0.2% to 2%. Three confirmed clinical and fatal cases of leishmaniasis in dogs of unidentified origin have been reported by our laboratory and the state veterinarians. The disease has been reported in neighbouring countries as well as the putative sandfly vectors. This study concluded that the risk for the introduction and degree of uncertainty of Leishmania in imported dogs in South Africa are moderate. Risk mitigation and recommendations such as investigations into possible occurrence of autochthonous leishmaniasis in the country, surveillance in its wildlife reservoirs and systematic surveillance of sandfly populations are discussed.
显示更多 [+] 显示较少 [-]Prevalence of gastrointestinal helminths and parasites in smallholder pigs reared in the central Free State Province 全文
2019
Ifeoma C. Nwafor | Hester Roberts | Pieter Fourie
Prevalence of gastrointestinal helminths and parasites in smallholder pigs reared in the central Free State Province 全文
2019
Ifeoma C. Nwafor | Hester Roberts | Pieter Fourie
Pigs are kept by farmers as a source of livelihood and food. Unfortunately, helminthiasis and other internal parasites are major setbacks to profitable pig production in Africa. There is a lack of information on the prevalence and intensity of gastrointestinal helminths and parasites plaguing resource-poor pig farmers in the Free State. Knowledge of these endemic parasites can be used as baseline data to help design future intervention plans. The aim of this study was to identify and quantify the types of gastrointestinal helminths and parasites prevalent in smallholder pigs reared in the central Free State Province. Faecal samples were randomly collected from 77 pigs and parasitologically analysed. Quantification was done using the McMaster counting technique. Farming system, age, gender and health status were the risk factors considered. The study was conducted between January and March 2016. Overall, results showed that 61 samples (79.2%) tested positive for one or more gastrointestinal parasites, which were observed as single or mixed infections. Amongst the positive samples, 44.5% were infected with Ascaris suum, 50.6% with Trichuris suis, 26.0% and 72.7% were infected with Oesophagostomum dentatum and coccidia, respectively. There were significant differences (p < 0.05) between the rate of infection in the intensive and semi-intensive systems and between the dewormed and non-dewormed pigs. Piglets and female pigs recorded a higher prevalence in their categories. Pigs excreted mostly low (eggs per gram [EPG] ≤ 100) to moderate (EPG > 100 < 500) levels of helminth eggs. It is concluded that different species of gastrointestinal parasites are present in most pigs reared by smallholder farmers in this study area.
显示更多 [+] 显示较少 [-]Prevalence of gastrointestinal helminths and parasites in smallholder pigs reared in the central Free State Province 全文
2019
Nwafor, Ifeoma C. | Roberts, Hester | Fourie, Pieter | Central University of Technology, Free State, South Africa.
Pigs are kept by farmers as a source of livelihood and food. Unfortunately, helminthiasis and other internal parasites are major setbacks to profitable pig production in Africa. There is a lack of information on the prevalence and intensity of gastrointestinal helminths and parasites plaguing resource-poor pig farmers in the Free State. Knowledge of these endemic parasites can be used as baseline data to help design future intervention plans. The aim of this study was to identify and quantify the types of gastrointestinal helminths and parasites prevalent in smallholder pigs reared in the central Free State Province. Faecal samples were randomly collected from 77 pigs and parasitologically analysed. Quantification was done using the McMaster counting technique. Farming system, age, gender and health status were the risk factors considered. The study was conducted between January and March 2016. Overall, results showed that 61 samples (79.2%) tested positive for one or more gastrointestinal parasites, which were observed as single or mixed infections. Amongst the positive samples, 44.5% were infected with Ascaris suum, 50.6% with Trichuris suis, 26.0% and 72.7% were infected with Oesophagostomum dentatum and coccidia, respectively. There were significant differences (p 0.05) between the rate of infection in the intensive and semi-intensive systems and between the dewormed and non-dewormed pigs. Piglets and female pigs recorded a higher prevalence in their categories. Pigs excreted mostly low (eggs per gram [EPG] ≤ 100) to moderate (EPG 100 500) levels of helminth eggs. It is concluded that different species of gastrointestinal parasites are present in most pigs reared by smallholder farmers in this study area.
显示更多 [+] 显示较少 [-]A field study on the efficacy of ivermectin via subcutaneous route against chewing lice (Bovicola caprae) infestation in naturally infested goats 全文
2019
Ajith, Y.(ICAR-Indian Veterinary Research Institute Division of Medicine) | Dimri, Umesh(ICAR-Indian Veterinary Research Institute Division of Medicine) | Gopalakrishnan, A.(Tamil Nadu Veterinary and Animal Sciences University Department of Veterinary Clinical Medicine) | Devi, Gopinath(ICAR-Indian Veterinary Research Institute Department of Veterinary Medicine)
Caprine pediculosis is an ectoparasitic disease of great concern among goat farmers in India. It may be caused by either sucking lice or chewing lice; the latter one results in severe skin lesions, leading to production loss. This study evaluated the effectiveness of the macrocytic lactone drug, ivermectin, administered via subcutaneous injection, against chewing lice Bovicola (Damalinia) caprae infestation in naturally infested goats. The study was conducted on 20 goats with severe B. caprae infestation. Animals of group A (n = 10) were treated using a single dose of ivermectin (200 µg/kg body weight) subcutaneously and animals of group B (n = 10) underwent placebo therapy using normal saline. The animals were examined on days 0, 3, 7, 14, 21, 28, 42 and 56 for lice counts. There was 100% elimination of lice in all animals of group A and effective protection from re-infection remained at least for 21 days. Considerable improvement in haematological parameters was also observed by day 21. Based on this study, ivermectin injected via a subcutaneous route can be used effectively for the therapeutic and prophylactic management of chewing lice infestation in goats maintained under an extensive grazing system.
显示更多 [+] 显示较少 [-]Sheep enteric cestodes and their influence on clinical indicators used in targeted selective treatments against gastrointestinal nematodes 全文
2019
Meradi, Salah(University of Batna Institute of Veterinary Sciences and Agronomic Sciences) | Cabaret, Jacques(François-Rabelais University National Institute for Agricultural Research) | Bentounsi, Bourhane(University of Constantine Institute of Veterinary Sciences)
Clinical indicators such as diarrhoea (DISCO) or anaemia (FAMACHA©) are used as a measure for targeted selective treatments against gastrointestinal nematodes (GIN). Enteric cestodes such as Moniezia may interfere directly with DISCO or indirectly with the FAMACHA© score. We investigated 821 Ouled Djellal rams naturally infected in a steppe environment (GIN alone, cestodes alone, GIN and cestodes) or not. The rams were treated with ivermectin 2 months before being slaughtered to reduce the impact of nematodes on the clinical scores; however, persistent or newly acquired GINs were not related to both scores. Of the non-infected rams (n = 296), 26% identified as needing treatment against GIN using the FAMACHA score, and 34.5% using DISCO would have been thus selected. This implies that the clinical indicators used for the targeted selective treatment of gastrointestinal nematodes are not fully reliable when a low infection is recorded and may well be influenced by confounding factors. As expected, only DISCO was affected by cestode infection, and we suggest that the presence of Moniezia should also be taken into consideration.
显示更多 [+] 显示较少 [-]Prevalence of gastrointestinal helminths and parasites in smallholder pigs reared in the central Free State Province 全文
2019
Nwafor, Ifeoma C.(Central University of Technology Faculty of Health and Environmental Sciences Department of Agriculture) | Roberts, Hester(Central University of Technology Faculty of Health and Environmental Sciences Department of Life Sciences) | Fourie, Pieter(Central University of Technology Faculty of Health and Environmental Sciences Department of Agriculture)
Pigs are kept by farmers as a source of livelihood and food. Unfortunately, helminthiasis and other internal parasites are major setbacks to profitable pig production in Africa. There is a lack of information on the prevalence and intensity of gastrointestinal helminths and parasites plaguing resource-poor pig farmers in the Free State. Knowledge of these endemic parasites can be used as baseline data to help design future intervention plans. The aim of this study was to identify and quantify the types of gastrointestinal helminths and parasites prevalent in smallholder pigs reared in the central Free State Province. Faecal samples were randomly collected from 77 pigs and parasitologically analysed. Quantification was done using the McMaster counting technique. Farming system, age, gender and health status were the risk factors considered. The study was conducted between January and March 2016. Overall, results showed that 61 samples (79.2%) tested positive for one or more gastrointestinal parasites, which were observed as single or mixed infections. Amongst the positive samples, 44.5% were infected with Ascaris suum, 50.6% with Trichuris suis, 26.0% and 72.7% were infected with Oesophagostomum dentatum and coccidia, respectively. There were significant differences (p < 0.05) between the rate of infection in the intensive and semi-intensive systems and between the dewormed and non-dewormed pigs. Piglets and female pigs recorded a higher prevalence in their categories. Pigs excreted mostly low (eggs per gram [EPG] ≤ 100) to moderate (EPG > 100 < 500) levels of helminth eggs. It is concluded that different species of gastrointestinal parasites are present in most pigs reared by smallholder farmers in this study area.
显示更多 [+] 显示较少 [-]Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey 全文
2019
Polat, Pelin F.(Harran University Faculty of Veterinary Medicine Department of Internal Medicine) | Şahan, Adem(Harran University Faculty of Veterinary Medicine Department of Internal Medicine) | Aksoy, Gürbüz(Harran University Faculty of Veterinary Medicine Department of Internal Medicine) | Timurkan, Mehmet O.(Atatürk University Faculty of Veterinary Medicine Department of Virology) | Dinçer, Ender(Mersin University Research and Application Center)
Canine parvovirus-2 (CPV-2) is the aetiological agent of an infectious viral disease of dogs, characterised by diarrhoea and vomiting. Mutations of the CPV-2 genome have generated new variants circulating worldwide. This article reports the molecular analysis of CPV-2 variants collected in the dog population in southeast Anatolia, Turkey. Twenty blood samples previously taken for the laboratory diagnosis of dogs with suspected parvovirus were screened for CPV-2 by polymerase chain reaction (PCR). Of the 20 samples, 18 tested positive for CPV-2. Partial VP2 gene sequencing and restriction fragment length polymorphism (RFLP) analysis revealed CPV-2a (n = 1), CPV-2b (n = 16) and CPV-2c (n = 1) variants. Phylogenetic analysis based on the partial length VP2 gene showed that CPV-2b (n = 15) variants showed sequences clustering separately in the phylogenetic tree. The CPV-2c sample was phylogenetically related to Chinese strains and Indonesia strain, whereas the CPV-2a sample was phylogenetically related to the Portuguese strain. These results, which are the first to demonstrate the presence of CPV-2c in the dog population of southeast Anatolia, Turkey, indicate that CPV-2a/2b/2c variants co-exist in Turkey's dog population.
显示更多 [+] 显示较少 [-]Serum biochemistry in cows of different breeds presented with reproductive conditions 全文
2019
Molefe, Keitiretse(North-West University Faculty of Natural and Agricultural Science Department of Animal Health) | Mwanza, Mulunda(North-West University Faculty of Natural and Agricultural Science Department of Animal Health)
Minimising health problems and increasing yield have always been the objectives in livestock agriculture. Hence, increases in incidences of reproductive conditions in cattle farming pose a great threat to productivity and impose undesirable economic implications. This study aimed to examine the concentrations of different biochemical compounds in cows with reproductive conditions. Seventy-seven blood samples were collected from cows at different rural areas around Mafikeng, following cases of downer cow syndrome, dystocia, retained placenta, vaginal prolapse and abortion. Means of serum metabolites across the different reproductive conditions were statistically compared using Pearson's chi-square test to determine variations of serum metabolites in cows of different breeds. In mixed breed cows, higher than normal calcium concentrations were observed in downer cow syndrome (25.25 ± 8.47) and dystocia (85.50 ± 8.46) cases. It was also observed that cholesterol concentrations were significantly low in abortion (2.52 ± 0.79), retained placenta (3.18 ± 0.61) and vaginal prolapse (2.37 ± 0.97) cases in Afrikaner cows. The study showed that Brahman (43.1%) and Afrikaner (43.1%) breeds were mostly affected by downer cow syndrome. Additionally, the occurrences of downer cow syndrome (53.9%) and abortions (60%) were mostly observed in cows of 1-3 years, in second and first parities, respectively. This study proves that concentrations of calcium, urea or blood urea nitrogen (BUN), magnesium and cholesterol are significantly altered in incidences of reproductive conditions in cows of different breeds. It is also shown that serum biochemistry is affected by reproductive conditions in cows of different ages and parity. This data serves as a tool that could be used to enhance research in animal production and reproduction.
显示更多 [+] 显示较少 [-]Molecular characterisation of infectious bursal disease virus in Namibia, 2017 全文
2019
Molini, Umberto(University of Namibia Faculty of Agriculture and Natural Resources School of Veterinary Medicine) | Aikukutu, Gottlieb(Central Veterinary Laboratory) | Kabajani, Juliet(International Atomic Energy Agency Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture) | Khaiseb, Siegfried(Central Veterinary Laboratory) | Cattoli, Giovanni(International Atomic Energy Agency Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture) | Dundon, William G.(International Atomic Energy Agency Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture)
Between July and September 2017, samples collected from six unvaccinated chickens in Namibia were shown to be positive for infectious bursal disease virus (IBDV) by RT-PCR. Partial sequence and phylogenetic analysis of the VP1 and VP2 genes from six viruses revealed that they all belong to the very virulent pathotype (Genogroup 3) and are genetically very similar to IBDVs identified in neighbouring Zambia. This is the first molecular characterisation of IBDV in Namibia and has implications on the control and management of the disease in the country.
显示更多 [+] 显示较少 [-]Effect of passive transfer of immunity on growth performance of preweaned dairy calves 全文
2019
Elsohaby, I. | Cameron, M. | Elmoslemany, A. | McClure, J. T. | Keefe, G.
The primary objective of this observational study was to examine the association between passive transfer of immunity and growth performance in preweaning calves. A secondary objective was to evaluate the utility of a heart girth tape (HGT) to estimate body weight (BW) in preweaning calves. A total of 142 Holstein calves were enrolled in this study. Blood samples were collected 24 to 48 hours after birth and serum immunoglobulin G (IgG) concentration for each calf was measured by radial immunodiffusion assay. Calf BW was determined at birth, at 21 days, and at weaning using an electronic scale (ES) and HGT. A significant positive association was detected between serum IgG and both BW at 21 days and average daily gain (ADG) from 0 to 21 days of life. Additionally, ADG from 0 to 42 days of life showed a trend toward an improved rate of gain as IgG concentration increased. The Pearson correlation coefficient between BW obtained from ES and HGT was 0.81 at birth, 0.86 at 21 days, and 0.83 at weaning. The mean differences between BW obtained from ES and HGT were -3.1 kg at birth, -3.2 kg at 21 days, and -7.7 kg at weaning. In conclusion, serum IgG concentration in neonatal calves is an important contributing factor for the variation in growth performance of preweaning calves. The HGT can be used to estimate the BW of preweaning calves but has a tendency to overestimate weight, especially at weaning compared to birth and 21 days of age.
显示更多 [+] 显示较少 [-]Comparison of glucose concentrations in serum, plasma, and blood measured by a point-of-care glucometer with serum glucose concentration measured by an automated biochemical analyzer for canine and feline blood samples 全文
2019
Lechner, Matthew J. | Hess, Rebecka S.
OBJECTIVE To determine the correlation between glucose concentrations in serum, plasma, and blood measured by a point-of-care glucometer (POCG) and serum glucose concentration measured by an automated biochemical analyzer (ABA; gold standard). SAMPLE 152 canine and 111 feline blood samples. PROCEDURES For each sample, the glucose concentration in serum, plasma, and blood was measured by a POCG and compared with the ABA-measured glucose concentration by means of the Lin concordance correlation coefficient. Results were summarized by species for all samples and subsets of samples with hyperglycemia (ABA-measured glucose concentration > 112 mg/dL for dogs and > 168 mg/dL for cats) and pronounced hyperglycemia (ABA-measured glucose concentration > 250 mg/dL for both species). The effect of PCV on correlations between POCG and ABA measurements was also assessed. RESULTS Hyperglycemia and pronounced hyperglycemia were identified in 69 and 36 canine samples and 44 and 29 feline samples, respectively. The POCG-measured glucose concentrations in serum, plasma, and blood were strongly and positively correlated with the gold standard concentration. The PCV was positively associated with the correlation between the POCG-measured blood glucose concentration and the gold standard concentration but was not associated with the correlations between the POCG-measured glucose concentrations in serum and plasma and the gold standard concentration. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that POCG-measured glucose concentrations in serum, plasma, and blood were strongly correlated with the ABA-measured serum glucose concentration, even in hyperglycemic samples. Given the time and labor required to harvest serum or plasma from blood samples, we concluded that blood was the preferred sample type for use with this POCG.
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