Objective: To evaluate the analgesic efficacy of ABT-116, a transient receptor potential cation channel vanilloid subfamily V member 1 antagonist, and compare it with that of buprenorphine by measurement of mechanical and thermal nociceptive thresholds in dogs. Animals: Six 7- to 8-month-old dogs (3 males and 3 females). Procedures: In a crossover study design, all dogs received ABT-116 (30 mg/kg, PO) and buprenorphine (0.03 mg/kg, orotransmucosally), with each treatment separated by 1 week. Physiologic variables were recorded prior to and 1, 6, and 24 hours after drug administration. Thermal (thoracic) and mechanical (dorsolateral aspect of the radius [proximal] and dorsopalmar aspect of the forefoot [distal]) nociceptive thresholds were assessed prior to (baseline) and 15 minutes and 1, 2, 4, 6, 12, 18, and 24 hours after treatment. Results: Buprenorphine administration resulted in higher overall thermal and proximal mechanical nociceptive thresholds, compared with ABT-116. Distal mechanical nociceptive thresholds after treatment were higher than baseline values for both treatments, but the magnitude of change was greater for buprenorphine at 1 hour after administration. Whereas HR and RR sporadically differed from baseline values after ABT-116 administration, rectal temperature increased from a baseline value of 39 ± 0.2°C (mean ± SD) to a peak of 40.6 ± 0.2°C at 6 hours. Conclusions and Clinical Relevance: In dogs without inflammation or nerve injury, PO administration of ABT-116 did not consistently result in an increase in nociceptive thresholds. However, clinically relevant increases in rectal temperature were identified after ABT-116 administration.

Objective: To characterize clinical and behavioral changes in calves following inoculation with Mycoplasma bovis and evaluate relationships between those changes and pulmonary disease. Animals: 22 healthy Holstein steers. Procedures: 20 calves were inoculated intranasally with < 10(8) CFU or > 10(9) CFU of M bovis. Calves were assigned a clinical illness score (CIS) on a scale of 1 through 4 twice daily on the basis of severity of cough, labored breathing, and lethargy. For each calf, distance traveled and time spent near the waterer, feed bunk, or shelter were determined via a remote location monitoring device. Calves were euthanized and necropsied 22 days after inoculation. Results: 13 calves became clinically ill after challenge inoculation; 3 calves were euthanized within 20 days. Among all calves, consolidation was evident in 0% to 79.9% of the lungs; extent of lung consolidation did not differ between the challenge dose groups. Distance traveled and percentages of time spent in proximity to the feed bunk and shelter were associated with CIS; calves with more severe disease traveled less distance and spent less time at the feed bunk and more time in the shelter. Distance traveled by calves was negatively associated with extent of lung consolidation (< or ≥ 10% of lungs affected); this effect was modified by trial day. Conclusions and Clinical Relevance: Following inoculation with M bovis, calf behavior patterns were associated with both CIS and severity of pulmonary disease. Use of behavior monitoring systems may aid in recognition of respiratory tract disease in calves.

The world widely distributed infection by Rhodococcus equi usually leads to pneumonia and associated respiratory signs. This study is aimed at detecting the occurrence of this pathogen in selected horse farms. A total of 12 R. equi isolates from few samples (13.89%) were successfully obtained from soil and faeces collected from two selected farms. However, based on the vapA gene classification, only one virulent R. equi isolate was identified.

Rice straw and oil palm empty fruit bunch were treated with urea&#xD;and commercial effective microorganisms to study their ability to breakdown fibres, improved in protein and energy values. In rice straw, the crude protein and crude fibre treated with urea and urea + effective microorganisms showed significant differences from the untreated control. The neutral detergent fibre and nitrogen free&#xD;extract values in the rice straw treatment did not show significant differences. Metabolizable energy values decreased with the treatment groups compared to the control. The results of keeping the treated rice straw in anaerobic plastic bags up to 30 days showed no significant breakdown of fibre components in the control and urea&#xD;treated groups. But there is a significant dropped in the neutral detergent fibre in urea + effective microorganisms treatment.&#xD;In oil palm empty fruit bunch, there were no significant differences seen in the crude protein, crude fibre, acid detergent fibre, neutral detergent fibre, nitrogen free extract and metabolisable energy values. Storage showed decreased in energy values in all treated groups. The addition of urea and microbes showed improved protein level and in the reduction of the fibre components in rice straw only. Keywords: rice straw, oil palm empty fruit bunch, urea, effective microorganisms, crude fibre, acid detergent fibre, neutral&#xD;detergent fibre, metabolisable energy.

A number of studies have measured collagen fibers and collagen&#xD;deposition in wound healing process with advances imaging techniques. However, these are performed by complicated methods and need specific tools. In search of the easier ways in routine histopathological laboratory, collagen measurement and staining pattern of wound healing process were observed in wounded skin of Sprague Dawley’s rat by using two different stains which are standard haematoxylin and eosin (H&E) and modified Masson’s&#xD;trichrome staining (MT). The comparison between these staining in wounded tissues was made to evaluate the advantages and disadvantages of both staining in wound healing study for 21 days postwounding. Tissues which stained with MT staining was then evaluated its collagen re-organization and density by using polarized light microscope with the aid of image analyzer software. Results showed that tissues stained with standard H&E could not be used to measure and differentiate the collagen deposition which is contradictory to MT staining. Wounded tissue stained with MT staining has showed a clear view of collagen fibers deposition&#xD;and re-organisation compared to H&E staining. This finding could validate the using of modified MT staining which leads to accurate histopathological analysis and observation in wound healing study.

A biogas plant is an anaerobic digester that produces biogas from organic materials such as animal waste, sewage slurry, vegetable waste and others. The Department of Veterinary Services had developed a small-scale biogas plant at a commercial dairy farm&#xD;in Semenyih, Selangor. The objectives of this project were to promote green technology and zero waste concepts on animal farming as well as to mitigate adverse effects on the environment due to unsystematic management of animal waste disposal. The biogas produced was used as fuel for biogas lamp, biogas stove, biogas water heater, biogas rice cooker and 1 kW biogas generator. The generator was connected to the distribution board in order to supply electricity to a few switch sockets and lighting system for farm use. A biogas plant in an animal farm is one of the green technology applications because it not only produces renewable gas and generates&#xD;electricity but also minimises greenhouse gas emissions and environmental pollution. Furthermore, the residual solid waste&#xD;produced at the end of the process can be dried to be used as organic fertiliser.

This study was carried out to investigate the prevalence of equine&#xD;lameness and metabolic disorders in endurance horses during an endurance race. Out of 67 horses that participated in the race, 19 horses completed the race successfully without any derangement while 48 horses were eliminated from the race for various disorders. Fifty-three (53.73%) percent of these horses had metabolic disorders and&#xD;17.91% were eliminated due to lameness. The study showed that the highest number of endurance horses that were eliminated were due to metabolic disorders followed by lameness. These findings may assist veterinarians in designing laudable measures in the management and&#xD;conditioning protocols of endurance horses during training and further prevent the morbidity and mortality during endurance races.

The study was carried out to investigate the effect of prolong&#xD;submaximal exercise on heart rate reflecting the performance of endurance horses after covering distances of 40 and 80 km races. This study was conducted in Malaysia to determine the post-race&#xD;heart rates of endurance horses based on distances for the eliminated horses and those that completed the races with good&#xD;performance. Heart rates of competing endurance horses were examined at pre and post-race. Thirty-four endurance horses were sampled; N = 9 (40 km) and N = 9 (80 km) were the good performance horses while, N = 3 (40 km) and N = 13 (80 km) were the poor performance horses&#xD;eliminated from the race. The mean heart rate of the good performance horses in the 40 km category was 53 ± 8 bpm and that of&#xD;the 80 km in the good performance category was 56 ± 7 bpm, while the eliminated horses of the 40 km group had 74 ± 17 bpm and those in the 80 km group had 78 ± 7 bpm. The study showed that eliminated&#xD;horses in the 40 and 80 km categories both exhibited high heart rates compared to the good performance horses that were in the same racing category. Thus, the heart rates of horses during training may be used to predict performance based on distances covered in endurance races.

Parasitic helminth infections in small ruminants are prevalent in South East Asia (SEA), limiting productivity and causing major economic loss for farmers. The hot, wet, tropical climate all year&#xD;round favours trichostrongylid infections, predominantly haemonchosis in sheep and goats. Commercial large scale farms, with&#xD;more than 300 animals, as well as small holders or backyard farmers with less than 50 animals face the debilitating effects of haemonchosis when they graze their animals as effective worm control is often hampered by anthelmintic resistance. In Malaysia, frequent and indiscriminate use of anthelmintics in the past has resulted&#xD;in the majority of the small ruminant population facing resistance to one or more anthelmintics. Several alternative methods of worm control are being employed by farmers; the most important and effective being cut and carry or zero grazing, where the animals are kept in pens and grass is cut and fed. In Cambodia and Myanmar,&#xD;ruminants are still tethered or stall fed with minimal drug use. In Indonesia and Thailand, commercial goat and sheep farms are fast expanding to produce breeder stock for the SEA market. However, up to&#xD;75% of the small ruminant population is still traditionally managed by small scale farmers. In most of SEA, the McMaster method for faecal worm egg counts is the only diagnostic test used to assess helminthosis in ruminants. There is an urgent need to increase awareness and information on the need for testing faecal samples regularly before drenching, conducting faecal egg count reduction tests on a yearly basis, use of the FAMACHA technique to enable&#xD;selective treatment of individual animals. The use of alternative worm control methods to manage helminthosis will help promote effective ruminant production with reduced drug use and ncourage “green” farming methods. Extension of research on local bioactive plants which may have the potential to control helminthosis may also be beneficial in the longer term.