Objective: This research work aimed to assess the histopathological features and degree of gas¬tritis severity in a rat model, induced by Helicobacter pylori infection after administering omepra¬zole, amoxicillin, and clarithromycin as the standard first-line eradication regimen. Material and Methods: Twenty-one male rats were adapted for 7 days and randomly divided into three equal groups. Group 1 was considered a negative control. Group 2 and Group 3 were treated as H. pylori-inoculated groups. Group 2 was set as a positive control. Group 3 was admin¬istered omeprazole, amoxicillin, and clarithromycin as a first-line eradication regimen. Gastric histopathological examination was conducted. The difference in the severity of gastritis among the groups was examined using the one-way analysis of variance test. The significance was deter¬mined to be p < 0.05. Results: Gastritis was found in all inoculated groups. The severity of gastritis was highest in Group 2 (p < 0.05). We could see a refinement in gastritis severity after administering omeprazole, amox¬icillin, and clarithromycin as a first-line eradication regimen (Group 3 vs. Group 2; p [J Adv Vet Anim Res 2021; 8(1.000): 158-163]

Objective: Previously, we have shown that predicted zymogen granule protein 16 homolog B (P-G3MZ19) existed in Bali cattle (Bos javanicus) saliva. It was suggested that P-G3MZ19 is a mem¬ber of the mannose-binding lectin family that plays an essential role in innate immunity. In the present study, we aimed to analyze the structure and ligand-binding of P-3MZ19 in Bali cattle saliva. Materials and Methods: Saliva of four adult healthy Bali cattle was collected, lyophilized, and subjected to two-dimensional (2-D) gel electrophoresis. The target spot of around 17 kDa related to P-G3MZ19 was excised for matrix-assisted laser desorption ionization time-of-flight mass spec¬trometer/time-of-flight mass spectrometer mass spectrometry analysis and sequencing. The structure and the ligand-binding of P-3MZ19 were analyzed using bioinformatics software pro¬grams published elsewhere. Results: Based on Iterative Threading ASSEmbly Refinement the 3D model of P-G3MZ19 was suggested to have similarities to exo-alpha-sialidase (EC 3.2.1.18); while its ligand-binding sites consisted of seven residues, i.e., 25aa-26aa (Gly-Gly), 95aa (Phe), 138aa (Tyr), 140aa (Leu), 141aa (Gly), and 143aa (Thr). Conclusion: The structure of P-G3MZ19 of Bali cattle saliva and its ligand-binding sites have been successfully determined by using bioinformatics techniques. The biological and immunological roles of the peptide are currently under investigation based on P-G3MZ19 synthetic peptides. [J Adv Vet Anim Res 2021; 8(2.000): 224-229]

Objective: The papers primary goal is to report the devastating impact of carbon tetrachloride (CCl4) on rat testicular tissue and the possible protecting function of propolis against CCl4 based on its free radical scavenging and inflammatory relief properties. Materials and Methods: A total of 24 adult male albino rats had been classified into four groups (six rats/group). Rats of group 1 served as control, whereas groups 24 received propolis (200 mg/kg/day), CCl4 (3 ml/kg/day), and propolis/CCl4, respectively. After 4 weeks, the collected sera were applied for the estimation of lipid profile and sex hormones. Also, histopathological picture, malondialdehyde, and tumor necrosis factor alpha (TNFα) gene profile was measured in collected testicular tissues. Results: The present information revealed a noteworthy change (p < 0.05) in lipid profile, decrease in testicular weight, testosterone, antioxidants values along with a prominent increase (p < 0.05) in estradiol, lipid peroxidation values, and expression of TNFα in rats administrated with CCl4 com¬pared to control. Moreover, the histopathological profile showed the degeneration of the epithe¬lium. Interestingly, propolis attenuated the destructive effect of CCl4 on rat testes. Conclusion: The examined dose of propolis reduced oxidation, and inflammatory reactions resulted from CCl4 exposure and proved that it might have a helpful part in free radicals inter¬ceded diseases. [J Adv Vet Anim Res 2021; 8(1.000): 70-77]

Objective: This study aimed to salvage the study population from the fatality that occurs due to iatrogenic injury to the thoracic cavitys pleural membrane. Materials and Methods: An experimental study of temporomandibular joint arthroplasty with costochondral graft was carried out on 72 healthy Oryctolagus cuniculus species of male rab¬bits. The rabbits were distributed into two age groups: growing (34 months) and adult (1218 months). All the procedures were carried out under general anesthesia with xylazine hydrochlo-ride and ketamine hydrochloride after calculating the doses, maintained by halothane and O2 inhalations. Out of 72 rabbits, 33 rabbits had accidental perforation of the pleural membrane observed that required a chest drain. Results: In this study, 21 (63.64%) rabbits received chest drain and salvaged. The rest of the rab¬bits (n = 12; 36.36%) that did not receive any chest drain and died. Most of the rabbits (n = 17; 81%) were under the growing group, weighing less than 2 kg and four (19%) were adult rabbits. Conclusion: This manual chest drain is life-saving for rabbits. It is a new addition to the advance¬ment of thoracic surgery on animals. It is cost-effective and safe. The developed customized drain¬age system may make it easier to harvest the costochondral graft-related experiments. [J Adv Vet Anim Res 2021; 8(1.000): 138-145]

Objective: The present study aimed to evaluate the impact of dietary turmeric or garlic extract on physiological responses, hematological parameters, antioxidants status, testicular functions, and health indices of heat-stressed rabbit males. Materials and Methods: Seventy bucks were distributed into seven experimental groups (ten/ group). A commercial diet (CD) was fed to rabbit males in the control group, while males in G2, G3, and G4 were fed CD with turmeric at 30, 60, and 90 mg/kg, respectively. Males in G5, G6, and G7 were fed CD with garlic extract at 50, 75, and 100 mg/kg, respectively, for 8 weeks. Results: The results showed that turmeric or garlic extract levels decreased ear temperature, res¬piration rate, germ cell apoptotic number, total cholesterol, triglycerides, malondialdehyde level, libido, and sperm of dead and abnormal tail (p < 0.05). The hemoglobin and red and white blood cells, platelets, total antioxidants capacity, mass motility, mitochondrial potential, normal, acro¬some reacted, normality, and a total functional fraction of spermatozoa and relative of epididymal weight were increased (p < 0.05). The increased packed cell volume percentage and initial semen fructose were significant only when 100 mg per kg garlic extract was supplemented. Conclusion: Phytochemicals extract supplementation can be an effective dietary supplementation to eliminate heat stress and improve health, oxidative capacity, and testicular functions of rabbit males. [J Adv Vet Anim Res 2021; 8(2.000): 256-265]

Objective: Present research aims to isolate, identify, and determine the virulence of the Streptococcus agalactiae (group B Streptococcus; GBS), isolated from popped eye disease affected Tilapia and Vietnamese Koi (V. Koi) fishes. Materials and Methods: A total of 330 fish samples were collected, of which Tilapia (n = 180) and V. Koi (n = 150), were collected from 35 affected ponds of four selected districts of Bangladesh. Isolation of the bacterium was done using different culture media (Nutrient broth, Plate count agar, Tryptic Soy Agar, and Blood agar), and identification by using various biochemical tests (con¬ventional and using API 20 Strep kit) and polymerase chain reaction (PCR) using primers against 16S rRNA gene of S. agalactiae. Antibiotic susceptibility of the bacteria was performed using seven different antibiotics disc (Tetracycline, Oxytetracycline, Chlortetracycline, Streptomycin, Ciprofloxacin, Gentamicin, and Neomycin). Virulence of the isolated S. agalactiae was determined by infecting healthy Tilapia and V. Koi fishes through experimental infection. Results: Isolated bacteria were found Gram-positive paired and chained cocci, β-hemolytic and non-motile. Findings of biochemical and serological tests indicate that the isolated bacterium belongs to Group B Streptococcus of Lancefield classification. PCR result also confirmed that the bacteria were S. agalactiae. The bacterial isolates possessed resistance property against all the seven antibiotics used in this study. The isolated GBS was found highly virulent and showed 80%90% mortality for Tilapia and V. Koi fishes in experimental infection within 16 days of post-infection. Conclusion: From the findings of this study, it may be concluded that isolated GBS from the Tilapia and V. Koi fishes were highly virulent and possessed multidrug-resistance properties. [J Adv Vet Anim Res 2021; 8(1.000): 14-23]

Objective: In this case report, we report for first the time the presence of porcine deltacoronavi¬rus (PDCoV) in Peru (October 2019) and Latin America, and we present a control and eradication program using feedback (exposure)/controlled homogenization in a pig farm. Materials and Methods: This farm is located in the eastern jungle of Peru. Initially, clinical signs that appeared to be infectious diarrhea were detected, but the disease rapidly progressed to green diarrhea, vomiting, and increased mortality in piglets. These symptoms were compatible with those produced by porcine epidemic diarrhea virus and transmissible gastroenteritis virus, but also with PDCoV. Because the disease could not be diagnosed by clinical signs and symptoms, analysis by real-time polymerase chain reaction was used. Implementation of a feedback/con¬trolled homogenization program was quickly planned, accompanied by the closure of the farm, animal and farm health strategies, and its respective monitoring. Results: At the farm level, between 1 and 9 weeks after application of the program, the samples were positive for PDCoV, but at week 10, they were negative. At week 12, the weaned and fatten¬ing piglets gradually became populated as negative animals. In the follow-up before the opening of the farm, all the piglets were negative. In the final verification, gilts (week 35) entered the breeding area as replacements only after being tested negative for PDCoV. Conclusion: A rigorous feedback/controlled homogenization program and complementary mea¬sures led to eradicating PDCoV from the farm. [J Adv Vet Anim Res 2021; 8(2.000): 300-306]

Objective: The present study aimed to explain the effect of fetal bovine serum (FBS) on the in vitro production of porcine embryos and the molecular effects of FBS on the growing of porcine embryos. Materials and Methods: Immature porcine oocytes were matured and fertilized in vitro. The resulting zygotes were cultured in porcine zygotic medium-3- until day 7 and FBS was added on day 4. Without FBS, it was treated as a control group. Quantitative real-time PCR and 2′,7′-dichloro-di¬hydro-fluorescein diacetate (H2DCFDA) molecular staining techniques were used to detect the expression patterns of apoptosis-associated genes and the accumulation of reactive oxygen species (ROS), respectively. Paired students t-test was used by GraphPad Prism statistical software. Results: FBS supplementation boosted blastocyst (BL) development and total cell count per BL substantially (p < 0.05). However, hatching and hatched BLs also increased in the FBS-treated group compared to the control. We also found that ROS accumulation in FBS-treated embryos was significantly reduced (p < 0.05) compared to the control group. The expression of the anti-apoptotic gene BCL-2 was significantly increased in FBS-treated BLs, but the pro-apoptotic gene, caspase-3 expression, was significantly reduced in FBS-treated BLs. Conclusion: Our results suggest that FBS supplementation in porcine culture media could increase porcine embryo production by decreasing ROS accumulation and increasing the anti-apoptotic gene expression in developing BLs. [J Adv Vet Anim Res 2021; 8(4.000): 589-596]

Objectives: The purpose of this study was to determine the species composition of Eimeria circu¬lating in Mymensingh district, Bangladesh, using Internal Transcribed Spacer 1 (ITS1) sequences in polymerase chain reaction (PCR) assay. Materials and Methods: Coccidian oocysts were isolated and sporulated in a solution containing 2% potassium dichromate from litter slurry collected from 13 commercially active broiler farms in the research region. Genomic DNA was isolated from sporulated oocysts and used to amplify the Eimeria species-specific ITS1 gene by PCR amplification. Electrophoresis of 1.5% agarose gel was used to visualize the amplified PCR products. Results: In the study samples from Mymensingh district, Bangladesh, the presence of Eimeria brunetti, Eimeria acervulina, Eimeria necatrix, Eimeria mitis, and Eimeria tenella was identified. Conclusions: The findings of this study may shed light on the zonal approach to chicken coccidio¬sis control. Additionally, it suggests that ITS1-based PCR might be used in the field to accurately identify Eimeria species. [J Adv Vet Anim Res 2021; 8(3.000): 489-493]

Objective: This study aimed to explore the seroprevalence of Brucella spp. in goats in some selected areas of Bangladesh. Materials and Methods: This study was conducted in different goat-populated regions of Bangladesh from July 2017 to June 2018. A total of 208 serum samples were randomly collected from goats in Jashore (n = 50), Jhenidah (n = 22), Tangail (n = 40), Savar (n = 46), Thakurgaon (n = 18), and Bandarban (n = 32) areas. The samples were subjected to determine the presence of antibodies against Brucella spp. by rose bengal plate test (RBPT) and competitive enzyme-linked immunosorbent assay (c-ELISA). Results: Overall, the seroprevalence of Brucellosis in goats was 4.33% (n = 9/208) by RBPT and 2.40% (n = 5/208) by c-ELISA. The seroprevalence of brucellosis on the basis of RBPT was 6% (buck: 0%, doe: 6%) in Jashore, 4.5% (buck: 0%, doe: 4.5%) in Jhenidah, 2.5% (buck: 0%, doe: 2.5%) in Tangail, 4.35% (buck: 0%, doe: 4.35%) in Savar, 6.25% (buck: 0%, doe: 6.25%) in Bandarban, and 5.56% (buck: 0%, doe: 5.56%) in Thakurgaon. On the other hand, the seroprevalence of brucel¬losis by c-ELISA was 4% (buck: 0%, doe: 4%) in Jashore, 4.5% (buck: 0%, doe: 4.5%) in Jhenidah, 3.13% (buck: 0%, doe: 3.13%) in Bandarban, and 5.56% (buck: 0%, doe: 5.56%) in Thakurgaon. Brucellosis was more prevalent (p > 0.001) in does aging 34 years. Conclusion: Goats from different areas of Bangladesh are caring antibodies against Brucella organisms. Further bacteriological investigations are necessary. [J Adv Vet Anim Res 2021; 8(1.000): 123-128]