Objective: This study was carried out to find out the immunolocalization of Interleukin 6 (IL-6) and Interleukin 10 (IL-10) in the testicular tissue of testicular dysfunction rat treated with secretome from human umbilical stem cells. Materials and methods: Rats were induced with cisplatin for testicular dysfunction condition. After that, the rats were grouped into two categories and were treated with secretome at 0.2 and 0.5 ml/kg BW once every week for 4 weeks. One week later, after the secretome treatment, the rats were sacrificed for histological evaluation using the immunohistochemical method. The preparation slides were examined using a light microscope and were analyzed descriptively and quantitatively. Results: There were no IL-6 and IL-10 immunoreactivities seen in the testicular tissue after cispla¬tin induction. However, the immunoreactivities of IL-6 and IL-10 were detected after secretome treatment, with both dosages of 0.2 and 0.5 ml/kg BW. These immunoreactivities were detected in the spermatogonia, spermatid/luminal tissue of seminiferous tubule, spermatogenic cells, and Leydig cells. In the cell calculation, the numbers of IL-6 immunoreactive cells were higher at the lower secretome dosage. In contrast, the numbers of IL-10 immunoreactive cells were higher at the higher secretome dosage. Conclusion: IL-6 and IL-10 can be localized in the testicular tissue of testicular dysfunction rat after secretome treatment. [J Adv Vet Anim Res 2020; 7(3.000): 514-520]

Objectives: The current research aimed at investigating growth performances and meat yield characteristics of commercial cockerels supplemented with antibiotics and probiotics to the diet. Materials and methods: A total of 1,350 commercial cockerels (ISA Brown) were indiscriminately distributed to 3 treatment groups, each having three replications of 150-day-old chicks based on completely randomized design. The basal diet was treated as the control, while the experimental groups receiving Enrofloxacin 1 gm/kg and Protexin 1 gm/kg feed were considered as antibiotic and probiotic groups, respectively. Bodyweight, feed intake (FI), feed conversion ratio (FCR), and other relevant characteristics were recorded weekly until 49 days of trial. In the end, similar number (10) of birds from each replicate group were slaughtered to determine the carcass characteristics. Results: Significantly, better results were found in the overall growth performances of the cock¬erels in the probiotic-fed treatment group. Highly significant differences were also found in live bodyweight, weight gain, daily gain, FI, FCR, survivability, dressing percentage, abdominal fat, breast meat, drumstick, and thigh weight in the probiotic-fed treatment group compared to the others. Conclusion: The results of the present study indicate that the inclusion of dietary probiotics has a superior performance to antibiotics and may have the potentiality to be used as an alternative growth enhancer in the diet of cockerels. [J Adv Vet Anim Res 2020; 7(3.000): 471-476]

Objective: This study was undertaken to compare the curative efficacy of marigold leaf paste and turmeric paste on healing the incised wound in sheep. The study also determined the anti¬microbial effects and histopathological changes in a wounds healing process treated with these medicinal herbs. Materials and Methods: Surgical wounds (n = 18) were created aseptically in the skin of the flank region of six healthy sheep dividing them into three experimental groups. Follow-up data were taken up to day 21. Different morphological characteristics of the wound and wound contraction (length and width) were recorded weekly. Samples were collected on days 1, 2, and 3 to test the antimicrobial effects and on days 1, 3, and 7 for histopathological studies. Results: Treatment with marigold leaf paste and turmeric paste resulted in a swelled wound area of 11.78 ± 0.38 mm and 11.52 ± 0.27 mm, respectively. The wound areas were comparatively lower than that of the control group (11.44 ± 0.20 mm). Moreover, the least elevation (2.44 ± 0.12 mm) of the sutured line from the skin surface was noted and compared between the marigold leaf paste and normal saline (2.74 ± 0.13 mm). Bacterial colonies in the nutrient agar medium cultured with swabs from the normal saline-treated groups wound area were found on day 1. On the con¬trary, bacterial colonies were absent on days 2 and 3 of treatment in the groups treated with both the paste of marigold leaves and turmeric. Less intense tissue reactions and higher keratinization of epithelium were shown in the group treated with marigold leaf paste than turmeric paste and saline-treated groups. Conclusion: Marigold leaf paste showed less tissue reaction and healed the wounds effectively. Thus, this paste could be used for the treatment of superficial wounds in sheep. [J Adv Vet Anim Res 2020; 7(4.000): 750-757]

Objectives: Aqueous extracts of Rhizophora mucronata and Avicennia marina leaves were inves¬tigated for their hepatoprotective potential in diabetic rats. Materials and methods: One hundred twenty male albino rats were randomly assigned to eight equal groups (n = 15). The first group (control) comprised normal healthy rats, while the second to fifth groups were intraperitoneally injected with a single dose of streptozotocin (STZ) [60 mg/ kg body weight (BW)] for induction of diabetes. Group 2 was kept as positive diabetic control, while groups 35 were orally treated with aqueous extracts of R. mucronata (400 mg/kg BW), A. marina (400 mg/kg BW) and with a combination of ½ a dose of the two plants, respectively, for six weeks. Groups 68 were non-diabetic rats that orally received aqueous extracts of R. mucronata (400 mg/kg BW), A. marina (400 mg/kg BW), and a combination of ½ a dose of the two plants, respectively, for 6 weeks. Results: STZ-induced diabetic rats showed a significant reduction in serum glucose and liver enzymes, increased serum insulin, Homeostasis Model Assessment of β-cells (HOMA-β), and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR). Histopathological and immuno¬histochemical examinations of the liver revealed improved pathologic criteria in the plant extract treated diabetic rats compared with the remarkable changes which had been seen in STZ-induced diabetic rats. Conclusion: This study suggests that the aqueous extract of R. mucronata or its combination with A. marina showed potent hypoglycemic and hepatoprotective effects for liver dysfunction, as well as histopathological and immunohistochemical changes in the liver of STZ-induced diabetic rats. [J Adv Vet Anim Res 2020; 7(1.000): 177-185]

Objective: This study aims for molecular identification of naturally growing Bacillus cereus strain from a unique source, able to survive, and alleviate heavy metals from the nature. Materials and Methods: Pure isolate from Murrah buffalo milk was prepared in B. cereus selec¬tive Polymyxin pyruvate egg-yolk mannitolbromothymol blue agar (PEMBA) medium through a cascade of contamination free subcultures. The morphological and biochemical tests were done prior to 16S rRNA gene sequencing for strain identification and further physiological tests. The test strain was inoculated in both solid and suspension culture medium supplemented individually with Cd, Cu, Ag, and Zn to reveal the qualitative and quantitative heavy metal tolerance proper¬ties, respectively. Finally, the data collected from the in vitro assessment was statistically analyzed Results: Molecular analysis revealed that the test strain was B. cereus BF2, which was motile, catalase positive and Gram positive rod. B. cereus BF2 was found significant at 0.3% bile salt toler¬ance [two-way analysis of variance (ANOVA)p value is < 0.0001] where, t-test p value is < 0.0002 between Control Group (CG) and TGR-1; p < 0.037 between TGR-1 and 2; p < 0.0014 between CG and TGR-2. Similarly, B. cereus BF2 was significant in pH tolerant up to 8.0 with p < 0.0115 (in scale p < 0.05). The heavy metal tolerance test revealed that the test metals could not stop the growth of B. cereus BF2 even after 24 h of incubation but partially suppressed the growth kinetics for let¬ting into stationary phase. Among the four heavy metals, Cd and Zn showed partial antagonism to the growth of B. cereus BF2. The survivability was highly significant in the medium supplemented with Zn (p < 0.0001) and Ag (p < 0.018). Conclusion: Bacillus cereus BF2 can survive in selective heavy metals with metal resistance and biodegradation capacity. [J Adv Vet Anim Res 2020; 7(1.000): 62-68]

Objective: The study obtained data on the potential of local feed ingredients, both in quantity, quality, and continuity and to observe the absorption power and ability of livestock to use feed designed in vitro. Materials and Methods: The method uses a survey method of potential sources of animal feed and calculates the carrying capacity of ruminants and nutritional analysis of feed ingredients with proximate analysis. Feed formulations were made based on proximate analysis results of four formulas (P1, P2, P3, and P4) and tested in vitro. Results: First, Nganjuk district has the highest of the local food potential with the production of agricultural waste and agricultural industries, reaching 802,341.94 tons/year. Second, the most top carrying capacity analysis reached in Tulungagung district, which reached 62,534 ST/year or 43% of the total population of ruminants. Third, the results of the study of the quality of local feed ingredients indicate that each type of feed material has the right and proper nutrition given to ruminants. Fourth, the in vitro testing included showing P1 feed that had a very significant effect (p < 0.01) on dry matter digestibility and digestibility of organic matter of cows, respectively, 74.69% and 73.39%. Conclusion: The in vitro technique of making animal feed can be developed in the areas that have the potential to produce agricultural waste and agricultural industries to increase the carrying capacity of livestock. [J Adv Vet Anim Res 2020; 7(1.000): 92-102]

Objective: The objective of this work was to study the normal musculotendinous structures of the ostrich foot. Materials and methods: Ten African apparent healthy adult female ostriches (Struthio camelus) were slaughtered, and the pelvic limbs were separated from the ankle joint. The different biomed¬ical scanning techniques including radiography, computed tomography (CT), magnetic resonance imaging, and ultrasonography were achieved. Then, the ostrich feet were freshly dissected. Results: The radiographs and CT images showed the bony components of the ostrich foot that revealed the presence of long tarsometatarsus and phalanges of the only developed third and fourth toes. The third digit was the longest and possessed four phalanges, whereas the shorter fourth toe contained five phalanges. The ostrich foot consisted mainly of tendons in addition to several small associated muscles. The extensor structures were the extensor digitorum longus tendon, Mm. extensor proprius digiti III, and extensor brevis digiti III and IV. On the other hand, the flexor structures were the flexor digitorum longus, flexor hallucis longus, flexor perforans et perforatus digiti III, and flexor perforatus digiti III and IV tendons. Furthermore, fibularis lon-gus tendon and two muscles (Mm. abductor digiti IV and lumbricalis) were related to the flexor tendons. Conclusion: The combination between the dissecting anatomy and the different biomedical scan¬ning techniques was of value in describing the normal anatomical course of the musculotendi¬nous structures of the ostrich foot, which aids in the diagnosis of any clinical abnormalities in these structures. [J Adv Vet Anim Res 2020; 7(2.000): 242-252]

Objective: The objective of this study was to investigate the intensity of phage infection caused by Phage SAvB14, which was isolated from dairy farms, depending on the initial number of Staphylococcus aureus cells in the medium. Material and methods: To evaluate the impact of the viable bacteria S. aureus var. bovis on the intensity of phage infection caused by Phage SAvB14, 1 mg of phagolysate (phage titer 105 CFU/ ml) was introduced in 9 ml of nutrient broth with an appropriate amount of daily culture of S. aureus var. bovis under study. The number of viable staphylococci was determined by total viable count/ml. Results: In this experiment, we found that the intensity of phages lytic activity was dependent on the number of sensitive bacterial cells in the volume of the culture medium. Effective phage ther¬apy requires a high concentration of phages in the medium (inflammation foci) for rapid contact of the virus with bacteria. Conclusion: When developing a phage drug to treat subclinical mastitis, it is necessary to increase the phage titer in the drug or its dosage compared to the clinical form, as there is a lower proba¬bility of phage contact with a susceptible microbial cell. Besides, at a high concentration of bacte¬ria, there is a gradual decrease in nutrients in the medium, resulting in phages going back to the condition of lysogeny. [J Adv Vet Anim Res 2020; 7(3.000): 509-513]

Objective: The present study aimed to assess for the first time, in Medina, the prevalence of Mycobacterium avium subsp. paratuberculosis in ruminants due to its potential zoonotic importance. Materials and methods: A total of 823 sera samples and 364 milk samples were used to deter¬mine the incidence of Mycobacterium avium subsp. Paratuberculosis (MAP) using the indirect Enzyme-Linked Immunosorbent Assay. Results: The seroprevalence of MAP was 11.1% in sheep and 13.8% in goats, while no infection was recorded among camels. MAP infection was not influenced by the animals gender, but it was influenced by its locality since the infection rate in local animals was higher than that in imported ones with a significant correlation (p < 0.05). MAP infection had a significant correlation (p < 0.05) with 2 years aged animals. On the other hand, the detection of MAP in milk revealed that 17 (13.8%) goats and 12 (4.9%) sheep were infected. The prevalence of MAP in milk samples was not influenced by either the animals age or locality. Conclusion: Sheep and goats may act as a reservoir for MAP to the Medina community. Since Medina is an active area of mass gatherings as a destination for pilgrims throughout the year, therefore, necessary control measures should be implemented to lower the economic losses, zoonotic infections, and the possibility of a global epidemic. [J Adv Vet Anim Res 2020; 7(1.000): 69-76]

Objectives: The present work was conducted to investigate the nutritional profile of the highly consumed beef luncheon in Egypt. Besides, the potential health hazards associated with the con¬sumption of luncheon were highlighted. Material and methods: A total of 60 beef luncheon samples were collected from Egyptian mar¬kets. They were classified into three classes: A, B, and C based on their prices. The collected samples were examined for their chemical composition by determining moisture, protein, fat, ash, carbohydrate, and energy percentage. The contents of trace elements were also investigated. Results: The obtained findings showed a comprehensive dissimilarity in the chemical composition. According to the fresh weight base, moisture, protein, fat, ash, and carbohydrate fluctuated from 56.97 to 64.52, 3.50 to 16.10, 4.73 to 13.39, 3.30 to 3.51, and 11.32 to 27.44% w/w, respectively. The highest price class A Egyptian beef luncheon had more accepted nutritive value and dietary energy content. All the examined classes were low in calcium, potassium, zinc, and magnesium. The target hazard quotient indicated that the trace elements did not present any risks for con¬sumers except for sodium. High phosphorous content and high phosphorusprotein ratio were observed in all categories which had a harmful health effect, hence named new cholesterol. Conclusion: Based on the information, this study is the leading work that deeply investigated the chemical composition of the Egyptian luncheon classes, and the obtained data could be beneficial to update the nutritional knowledge used by dietitians and the responsible for nutrition assess¬ment and surveillance by the government. [J Adv Vet Anim Res 2020; 7(2.000): 299-307]