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Phagocytosis, bactericidal activity, and oxidative metabolism of milk neutrophils from dairy cows fed selenium-supplemented and selenium-deficient diets.
1990
Grasso P.J. | Scholz R.W. | Erskine R.J. | Eberhart R.J.
Six primiparous Holstein cows were fed a Se-deficient diet, beginning at least 90 days before their first calving, and 6 other primiparous cows were given the same diet plus a supplement of 2 mg of Se/cow/d as sodium selenite. All cows were fed their diets for the duration of the experimental period. One uninfected quarter of each cow was injected with 25 microgram of Escherichia coli endotoxin at postpartum week 5. Leukocytes were isolated by centrifugation from milk collected at postinjection hour 16. Isolated cells were 92 +/- 3% neutrophils and were incubated with Staphylococcus aureus or E coli in a 1:300 ratio. Phagocytosis and intracellular killing by neutrophils were assessed after 0, 30, 60, and 90 minutes by a fluorochrome assay, using acridine orange. Viability of neutrophils was assessed by use of trypan blue. Superoxide anion production and hydrogen peroxide production by neutrophils also were determined. Cows fed Se-deficient diets had significantly (P < 0.05) lower blood Se concentration and blood glutathione peroxidase activity than cows fed Se-supplemented diets. Selenium status had no effect on the phagocytic capacity of neutrophils. Neutrophils obtained from cows fed Se-supplemented diets killed a significantly (P < 0.05) higher percentage of ingested bacteria than did neutrophils from cows fed the Se-deficient diet. Viability was significantly (P < 0.05) reduced by incubation with S aureus in neutrophils from both groups of cows, with neutrophils from Se-deficient cows having lower viability. Superoxide anion production did not differ significantly between neutrophils from the 2 groups, but extracellular hydrogen peroxide concentration was significantly (P < 0.05) higher in neutrophils harvested from milk of cows fed the Se-deficient diet.
显示更多 [+] 显示较少 [-]Pathogenicity, hemagglutinability and the effect of physicochemical agents on virus of rabbit hemorrhagic disease.
1990
Yoon I.J. | Jeon Y.S.
Studies on the early diagnosis of pregnancy of dairy cows by EIA-kit of progesterone in milk.
1990
Kim M.K. | Shin H.J. | Lee M.H. | Lee M.H. | Kim S.K.
Effects of ammonia water on sporulation of coccidial oocysts originated from bovine.
1990
Wee S.H. | Kang Y.B. | Jang H. | Lee H.S. | Choi S.H.
Light microscopic observations on the in vitro effects of praziquantel on Heterophyopsis continua.
1990
Woo H.C. | Suh M.D. | Hong S.J.
Avidin-biotin complex for immunohistochemical diagnosis of Aujeszky's disease and hog cholera.
1990
Kim S.B. | Sur J.H. | Moon U.G.
Immunohistochemical identification of Newcastle disease virus with indirect immunoperoxidase technique.
1990
Nho W.G. | Sur J.H. | Kim S.B.
Antagonism of xylazine-induced hypotensive effect by yohimbine in rabbits.
1990
Shin D.H.
Fine structure of Theileria sergenti merozoite in Korean native cattle.
1990
Baek B.K. | Kim B.S. | Lee H.I.
Cultivo de Spirulina maxima ao ar livre. 1: inverno.
1990
Ruiz R.L. | Mos E.N. | Lima C.G. de | Ribeiro M.A.M.
