Results of studies of peculiar properties of progesterone content in blood serum of outbred female rats under the influence of intensive ionizing radiation against the background of stress stimulation realized in the conditions of the Republic of Belarus were presented. There were the following animal groups: 1. controlled rats; 2. irradiated rats; 3. stressed animals; 4. rats subjected to ionizing radiation and stress. Research results showed that concentration of progesterone in serum blood of female rats in 3 days after immobilized stress decreased on 40% in comparison with the control indexes. More intensive decreasing of hormone level took place after irradiation in dose of 1 Gy (on 62% in comparison with the controlled group). Combine activity of ionizing radiation and stress involved the decreasing of hormone concentration on 25% in comparison with the controlled group. In ten days there was the restoration of changes. Increase of progesterone concentration in blood serum took place in all tested groups. Hormone level in blood serum of irradiated rats increased on 46,04 nmol/l in comparison with results of the third day after irradiation. Hormone level in blood serum of stressed animals increased on 11,63 nmol/l, in group subjected to the combined activity of ionizing radiation and stress – on7,65nmol/l. On the thirtieth day the indexes of progesterone concentration in blood serum of irradiated rats, stressed rats and rats subjected to ionizing radiation and stress were almost similar to the control indexes

Methods of increasing the efficiency of semen fertilizing capacity in vitro with application of hormonal and biophysical methods of influence, as well as the determination of metabolic criterion of spermium viability were studied in the conditions of the republic of Belarus. Research object was frozen-deiced sperm of cattle. Research results showed that entering of 50 mkg/ml of prostaglandin into capacitation media increased the breaking level on 2,3-3,1%, but at the same time there was the decreasing of embryo output at the pre-implantation stages. Increasing of estrophan concentration in media for capacitation up to 100 mkg/ml, and its addition into media for fertilization made it possible to increase the embryo output at the stage of morula-blastocyte up to 1,6-18,5%. Application of 8mg/ml of caffeine as a capacitation agent for the preparation of cattle sperm for fertilization in vitro made it possible to obtain embryo output of 16,7% with breaking level of 25,9%. Influence of directed polarized light on semen after its maturation was more efficient in comparison with the influence on it just after swim-up procedure; the output of pre-implantation embryos was 16,7% against 12,9%. Application of laser radiation made it possible to get 14,7 % of morula-blastocytes. Intensity of sperm breath (0,41-0,63 tg), intensity of lipid peroxidation (0,88-0,97 conditional units /10E6 cells), intracellular content of adenosine triphosphate (0,97-1,60 Nm/10E6 cells) and membrane potential (33-35 uV) in the conditions of matured in vitro oocyte made it possible to get 16,4-17,3% of pre-implantation embryos with the breaking level of 39,8-42,3%

Determination of degree and relations between derangements of metabolic processes in calves with respiratory syndrome in the milking period was realized in the conditions of the Republic of Belarus. Research results showed that the calves with the respiratory syndrome had expressed metabolic processes disturbances, which were marked in the analyzed biochemical blood indexes (total protein; albumins; urea; creatinine; glucose; cholesterin; triglycerids; bilirubin; ALP; AAT; SGPT; calcium; phosphorous; magnesium; iron; manganese; cobalt; cuprum; zinc). In calves with respiratory syndrome there was noted the increased content of crude protein in blood serum (71,42 +/- 2,57 g/l P less than 0,05), low urea level (3,08 +/- mmol/l). There was the increased level of creatine (574,11 +/- 65,26 mkmol/l; P less than 0,01) and bilirubin (8,11 +/- 1,15 mkmol/l; P less than 0,05). Glucose concentration in blood of diseased calves was at below standard level (3,49 +/- 0,69 mmol/l). Glucose indexes varied and were changing during a day; and depended on muscular activity, intervals between feeding and hormonal regulation. Diseased calves had high cholesterol level in blood serum (4,07 +/- 0,34 mmol/l; P less than 0,05), which was stated in case of liver disease and hypothyroidism. Phosphor level was higher than the standard indexes of healthy calves (2,85 +/- 0,03 and 2,05 +/- 0,05, respectively). Content of magnesium, cobalt and zinc in blood of the diseased calves was lower than in healthy ones. Calves with respiratory pathology had protein, fat, hydro-carbonic and mineral metabolic processes

Study of peculiar features of protein metabolism of healthy colts in the first three months of life was realized in the conditions of the Republic of Belarus. Research results showed that there was a tendency of increasing of hemoglobin concentration up to 150,270+/- 4,73 g/l, number of total protein of blood serum – up to 60,33 +/- 1,33 g/l, and the decreasing of uric acid up to 97,33 +/- 10,72 micromole /l. Quantity of beta-1 and alpha globulin fractions of serum protein fluctuated a little – from 10,78 +/- 3,69% up to 8,44 +/- 1,24% and from 25,12 +/- 4,84% up to 22,06 +/- 4,93%, respectively. The most essential changes of protein metabolism were stated at two month old colts, which had urea concentration of 5,25 +/- 0,23 micromole/l, alanine aminotransferase activity – 66,92 +/- 3,16 units/l, creatine content increased up to 77,37 +/- 5,83 micromole /l; and there was the lowering of aspartate transaminase level up t159,5 +/- 8,1units/l. There was stated the lowering of albumin quantity up to 32,07 +/- 2,19%, and increasing of alpha-1, alpha2- and beta2- globulin fractions up to 7,52 +/- 1,23%, 14,98 +/- 1,41%, 11,82 +/- 1,04%, respectively. At the age of three months the quantity of urea and creatinine in blood serum of colts remained at the same level, alanine aminotransferase activity decreased on 21,56%, and aspartate transaminase increased up to 204,33 +/- 10,37 units/l. At this period the quantity of albumin fractions was 44,56 +/- 6,59%., concentration of alpha-1, alpha2- and beta2- globulins decreased on 10,64%, 30,04% and 34,52%, respectively

The problem of creation of improved conditions for cattle oocyte maturation in vitro was studied in the Republic of Belarus. The objects of study were cattle oocytes and embryos of early preimplantation stages of development obtained in vitro conditions. Research results showed that application of bovine serum in complex with embryonic or fetal serum in the process of oocyte cultivation in vitro conditions made it possible to obtain 35,5-44,8% of breaking down cells and 16,1-17,2 % of embryos at the stage of morula-blastocyte. Entering of 0,02 ng/ml of gonadotropin-releasing hormone (Surfagon) into media for oocyte maturation at the cost of regulation of the processes of growing and development of oocyte-cumulus complexes made it possible to increase of yield of matured till the stage of metaphase II oocytes up to 89,3 %. At the same time, the yield of divided embryos after fertilization increased on 2,5 % in comparison with control indexes and amounted 45,2 %. Research results showed that the synthetic phytohormone epibrassinolide could be used as a biologically active factor in the process of getting early embryos in vitro in concentration of 2 x 10E-7 – 2 x 10E-9 mole/l. It made it possible to get 46,4-55,0 % of divided cells and 14,2-16,2% preimplantation embryos

In the conditions of the Republic of Belarus there was produced a trial series of hyperimmune serum against livestock animals colibacillosis and there was realized the quality control of the analysed biological preparation. Research results proved that the developed serum was sterile, safe and had high immunogenetic activity. In course of quality control there were considered the following indexes: determination of physiognomy, colour, presence of visually accessible particulate matters; determination of safety and harnfullness; determination of activity; determination of concentration of hydrogen ions; determination of sterility; determination of serum volume in consumption package. For serum production there were used strains E.coli O9, O78, O0, O117, O26, O139, O15, O41, O55, O115, O141, O8, K88, K99, F41, 987P. For the production of trial series there was realized cultivation of Escherichia on the special nutritive media. There used bulls as serum producers

Investigation of spreading of diseases of micronutrient deficiencies at cow breeding farms of Grodno district of the Republic of Belarus depending on the season and character of polymicroelementoses, as well as the determination of relations between polyhypomicroelementoses and state of new born calves were realized. Clinical investigation of 1350 milked and calf-bearing dry cows and biochemical analysis of 450 blood samples taken at nongrazing time (before cows pasture) and at pasture time were conducted. The following animal groups were selected: the first group – cows after calving; the second group – the cows of three month pregnancy; the third group – calf-bearing dry cows. The following groups were selected among calf-bearing dry cows in nongrazing and pasture time: clinically healthy cows, which had biochemical blood changes, showing development of subclinical polyhypomicroelementoses (n = 20); clinically healthy cows with blood indicators, standing in limits of physiological standards. Number of calves was considered after calving of cows of both groups. Body weight, realization of staying and sucking position, evidence of sucking reflex, and calves disposition to neonatal pathology were considered in the study. Research result showed that cow microelementoses were marked by the development of subclinical polyhypomicroelementoses. Among cow polyhypomicroelementoses there was noted the domination of states followed by lowering of zinc, cobalt and cuprum content. Polyhypomicroelementoses were developing both in winter housing season and in course of grazing season. Polyhypomicroelementoses involve cows of different physiological states and were spreading more intensively at cow dry period and after calving. Calves obtained from cows with polyhypomicroelementoses often had the state of hypotrophy and high incidence of dyspepsia at colostric period. Clinical and biochemical status of the analyzed calves was characterized by signs of intoxication and hepatic insufficiency

Investigation of acute and subacute toxicity of formulations of elecampane (Inula helenium) (herbal infusion, tincture, liquid and dry extracts) by the example of experimental white mice of both sexes ( body weight - 18-20 g) and white rats (body weight - 180-200 g) was realized in the conditions of the Republic of Belarus. In course of studies there were analyzed behavior (excitation or depression), physical activity, habitus, appetite, degree of reaction revelation to exogenous irritants, presence of shivering, cramps, paresis, comatose state, time and character of intoxication, dates of animal death). Results of the toxicological experiments showed that the developed preparative forms of elecampane could be referred to the substance hazard category 4, i.e. low-hazardous substances (lethal dose (LD) sub(50) more than 5000 mg/kg live weight). Median lethal dose of tincture was 14043 mg/kg for mice and 12684 mg/kg for rats. Median lethal dose of liquid extract was 13230 mg/kg for mice and 12285 mg/kg for rats

Studying of toxicity of enterosorbent produced the basis of mixture of filtering pulvis of perlite, kizelgur and activated carbon was realized in the conditions of the Republic of Belarus by the example of healthy white mice of both sexes with body weight of 18-25 grams. Animals were divided in three groups. The tested substance was entered insitu to ventricle on an empty stomach after 12-hours of absolute diet. The first group animals were administrated the enterosorbent in doze of 5 g/1 kg, the second group animals - 2,5 g/1 kg. Glucose content increased from 3,8 +/- 0,47 to 4,3 +/- 0,17 mmol/l on the 30-th experimental day and decreased on 8,2 % on the 60-th experimental day. Cholesterin and triglyceride content lowered on 9,5 % and 11,1 %,respectively. Enzymes concentration of aspartate transaminase and alkaline phosphatase decreased from 120,0 +/- 3,87 up to 117,0 +/- 2,58 units/l and from 396,3 +/- 3,78 to 345,3 +/- 2,97 units/l, respectively. Urea increased from 3,7 +/- 0,16 to 3,9 +/- 0,17 mmol/l on the 30-th experimental day. Research results showed that mixture of filtering pulvis after the single oral administration in dose of 2,5-5 g/1 kg did not cause mice death. The analyzed mixture could be referred to the class of low-toxic substances. The mixture did not have irritation action and did not render chronic toxic action on the experimental mice

Scientific and production examination of feed additive Semerik-Vita containing iodine and selenium was realized in the conditions of the Republic of Belarus by studying of its influence on production and economic indexes of laying hens and their products, as well as by studying of its influence on clinical state and biochemical blood indexes of poultry. Research results showed that application of feed additive Semerik-Vita made it possible to increase of egg producing ability intensity on 1,8 %, egg yield - on 2,4 % in the conditions of lowering of feeding expenses on 1,4 %. Application of the analyzed feed additive made it possible to obtain a new kind of eggs fortified with iodine, selenium, beta-carotene and vitamins (A, E, C, PP, B1, B2, pantothenic acid and folic acid). In eggs of poultry of experimental group the content of selenium increased in 2,1 times, iodone - in 1,5 times, vitamin E – in 3,9 times, beta-carotene – in 1,4 times, vitamin A – in 1,3 times, pantothenic acid – in 1,2 times, vitamin C – in 1,17 times, vitamin PP – in 1,4 times, vitamin B1 – in 1,5 times, vitamin V2 – in 1,4 times, folic acid - in 1,5 times in comparison with eggs of laying hens from the control group. Research results proved that application of Semerik-Vita did not render negative effect on the clinical indexes of laying hens. It made it possible to lower the content of urea acid in 1,7 times, as well as to increase the content of triglycerides in 2 times, cholesterol - in 2,4 times, magnesium - in 1,28 times