A 5-year-old castrated male domestic shorthair cat was referred for further investigation of pulmonary nodules incidentally detected on thoracic radiographs. Thoracic radiographs identified ill-defined soft tissue opacity nodules with small faint mineral opacity. Thoracic computed tomography (CT) revealed that bronchial dilation and bronchial wall thickening in bilateral cranial and accessory lung lobes. Round to ellipsoid mineralized concretions were found embedded in the intrabronchial soft tissue attenuation material. Based on radiography, CT, and cytology, the patient was tentatively diagnosed as having broncholithiasis with chronic bronchitis. This report described the CT diagnosis of broncholithiasis, which has been rarely reported in a cat.

Vaccination against Newcastle disease (ND) is the most effective means of controlling the disease, and these vaccines are commercialized only after their safety and effectiveness have been verified through tests that comply with Korean Standards of National Lot Release for Veterinary Biologics. This study investigated whether a relatively convenient and safe serological test can be used in place of the challenge test using highly virulent ND virus. Hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA) were considered positive of log2 2 or more and cutoff value of 200 or more, respectively, in both live and inactivated vaccines. However, when the antibody levels of the live and inactivated vaccines induced using the Ulster 2C, KBNP-C4152R2L, and K148/08 strains were compared, the antibody titers for inactivated vaccines were significantly higher than those for live vaccines in both the HI assay and ELISA. A strong positive correlation was observed between HI and ELISA antibody titers. The live vaccines corresponded to a survival rates of ≥ 80% and the inactivated vaccines corresponded to 100% survival rates. This study confirmed that standard efficacy tests can serve as serological tests, and can replace the challenge test and that the vaccine approval process can be improved.

We describe four cases of feline progressive histiocytosis (FPH) including three females (one intact, two spayed) and one castrated male cat, with a mean age of 5.95 years at diagnosis. Masses were found under the skin of head, lip, neck, and vulva. Histologically, proliferative round cells had ovoid nuclei, foamy eosinophilic cytoplasm, distinct cytoplasmic processes, and numerous mitotic figures. Immunohistochemically, all cases were positive for Iba1 and MHC II (Dako). One case showed cytoplasmic positive staining for E-cadherin. To the best of our knowledge, this is the first documented report of FPH in Korea.

Infectious bronchitis virus (IBV) and avian reovirus (ARV) cause significant losses in the poultry industry throughout the world. A cross-sectional study was conducted in four villages in Manjacaze district, Southern Mozambique, to determine the seroprevalence of IBV and ARV. A total of 467 serum samples from adult unvaccinated backyard chickens were screened using commercial and competitive enzyme-linked immunoabsorbent assay kits. Our results showed anti-IBV and anti-ARV antibodies in all surveyed households and villages. The overall seroprevalence was 89.5% (95% confidence interval [CI]: 77.2–97.4) and 95.7% (95% CI: 88.0–99.2) for IBV and ARV, respectively. The risk of becoming exposed to IBV was lower in Chidenguele village compared with the other three villages (p  0.05). However, no statistically significant differences were observed for becoming exposed to ARV between villages (p  0.05). The backyard chickens tested in this study had no previous history of vaccination, outbreaks or typical clinical signs of IB and AR diseases. Therefore, the presence of antibodies to IBV and ARV was considered clear evidence that the birds have been naturally exposed to those two infectious agents, and the infection was of subclinical type. It is concluded that IBV and ARV are widespread in backyard chickens in the studied area. These obtained data are essential for design and implementation of chicken health development programmes. Contribution: The epidemiology of IBV and ARV of backyard chicken in Mozambique is unknown. This study determined the seroprevalence of IBV and ARV in backyard chicken health. The obtained data are essential for design and implementation of chicken health development programmes.

In this study, it was aimed to evaluate the levels of interleukins such as IL-1β, IL-6, IL-10 and IL-12β in sheep with Jaagsiekte by immunohistochemical methods. In this way, it will be revealed whether interleukins are effective in the progression of Jaagsiekte and how useful they are in the diagnosis of the disease. The material of the current study consisted of lung tissues of 26 sheep (Control, n=6 and Jaagsiekte, n=20) brought to the Department of Pathology for routine histopathological diagnosis. Tissue samples taken were fixed in 10% buffered formaldehyde solution. 5 µm-thick sections were taken from the paraffin blocks prepared after routine tissue follow-up procedures. Hematoxylin & Eosin staining was applied to the sections in order to detect histopathological changes. Sections were examined and photographed under a light microscope. The routine streptavidin–biotin peroxidase complex method was used.In sheep with Jaagsiekte, tumoral foci with large and small acinar or papillary growths were observed in the alveolar and bronchiole lumens. The control group was negative for IL-1β, IL-6, IL-10 and IL-12β immunoreactivity. IL 1β-6-10 and 12β levels were dramatically increased in the Jaagsiekte group compared to the control group.It was determined that interleukins were produced from tumoral cells and tumor microenvironment elements, and these interleukins showed pro-inflammatory effects, except for IL-10.

This study aimed to monitor oxidative stress parameters including total antioxidant status (TAS) and total oxidant status (TOS), and tumor necrosis factor-alpha (TNF-α) level during the healing process in hair goats with metritis. This study was carried out on a total of 25 hair goats with metritis (n=10; Group 1) and healthy (n=15; Group 2). The beginning of the study was accepted as day 0. In both Group 1 and Group 2, blood samples were collected on days 0 (first measurement day), 14 (second measurement day) and 28 (third measurement day) of the study for TAS, TOS and TNF-α analyzes. In addition, oxidative stress index (OSI) was calculated using TAS and TOS values.It was found that TAS level decreased in Group 1 compared to Group 2 at the first measurement day (p<0.05). However, TOS, OSI and TNF-α levels increased in Group 1 compared to Group 2 (p<0.01). At the second measurement day, TOS and OSI values were higher in Group 1 than Group 2 (p<0.05).In conclusion, the antioxidant system weakened, oxidative stress and TNF-α levels increased in animals with metritis compared to healthy animals. However, at the third measurement time, all parameters became similar between groups.

Objective: This study aimed to reveal the coronary veins and the branches that join it to provide venous drainage of the heart in Southern Karaman sheep. Material-Method: Eight Southern Karaman sheep’s heart tissues were used in the study. Latex injection techniques were used to determine the coronary veins and the branches that join it for providing venous drainage of the heart.Result: In the study, vena cordis magna, vena cordis media, and vv. cordis dextra was observed as venous drainage providing vessels. The vena cordis magna began in the lower third of the sulcus interventricularis paraconalis, called vena interventricularis paraconalis. This vein reached the sulcus coronarius and continued as vena circumflexus sinister. Vena cordis media was named as vena interventricularis subsinosus in sulcus interventricularis subsinosus. The vv. cordis dextra was responsible for the venous drainage of the facies atrialis of the heart.Conclusion: In the evaluation of the study findings, it was determined that the coronary veins and the branches joined it of Southern Karaman sheep were mainly similar to other sheep breeds in the literature. Still, there were some anatomical differences, for example; vena distalis ventriculi sinistri was opening into vena circumflexus sinister, vena apicis cordis was absent, vena semicircumflexa dextri was present.

To investigate the possible effect of N-acetylcysteine (NAC) treatment on rat diabetes-induced liver damage and immune reactivity of asprosin hormone in the liver. Twenty-eight Wistar albino male rats were used in the study. They were separated into 4 groups as Control (n=7), Diabetes (n=7), Diabetes+NAC (n=7), and NAC (n=7). The rats in all groups were dissected after the treatment, and liver tissues were taken for pathological examination. Tissue sections were stained with immunohistochemistry for detecting asprosin immunoreactivity, hematoxylin-eosin and picrosirius red staining were performed to determine the changes in the tissues.In the microscopical examination of hematoxylin-eosin-stained sections normal histological hepatic tissues were seen in the Control and NAC groups. Pathological examination of liver tissue from diabetic rats showed marked dissociation, fibrosis, degeneration, inflammation, necrosis, Kupffer cells activation, bile duct proliferation, and congestion. A significant decrease in these lesions was observed in the DM+NAC group. Immunohistochemical studies showed that asprosin immunoreactivity was increased in the DM group in a significant manner. Asprosin expression was observed to be significantly reduced in the DM+NAC group in comparison to the DM group.Our findings show that NAC administration reduces liver damage in diabetic rats and can be used to reduce/eliminate the negative effects of diabetes in rat liver tissue..

This study aimed to investigate the effects of Chia (Salvia hispanica L.) oil and Dandelion (Taraxacum Officinale) extract on Tumor Necrosis Factor-α (TNF-α) and Interleukin 6 (IL-6) release in liver tissue of diabetic rats. Experimental groups were created as control, sham, chia, dandelion, diabetes (DM), diabetes+chia (DC), and diabetes+dandelion (DD). Body weight and blood glucose measurements were taken on the 1st, 3rd, and 17th days of the study and evaluated statistically. A one-way ANOVA test was performed to determine the differences between the groups. The Duncan test was used to compare significant differences between groups. At the end of the study, Masson's trichrome staining and Hematoxylin-Eosin staining were employed for histological examinations of liver tissues, and the distribution of TNF-α and IL-6 was examined by applying the Streptavidin-biotin peroxidase method.It was determined that body weight and blood glucose measurements were significantly decrease for the DC group compared to other groups. Immunoreactivity of TNF-α and IL-6 was found to decrease in DC and DD groups at close to the control levels.Based on our results, it was thought that the use of chia and dandelion in diabetes may contribute to the alleviation of disease-related complications by having a positive effect on proinflammatory cytokine levels.