OBJECTIVE To investigate the in vitro effects of clinically relevant concentrations of the local anesthetics (LAs) bupivacaine, lidocaine, lidocaine with preservative (LP), mepivacaine, and ropivacaine on equine chondrocyte and fibroblast-like synoviocyte (FLS) viability. SAMPLES Chondrocytes and FLSs of the metacarpophalangeal joints of 4 healthy adult horses. PROCEDURES Viability of chondrocytes and FLSs was determined with 3 assays: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH), and trypan blue (TB) exclusion (only FLS). Viability was assessed after 30- and 60-minute exposures to 0.0625%, 0.125%, and 0.25% bupivacaine; 0.25%, 0.5%, and 1% lidocaine; 0.25%, 0.5%, and 1% LP; 0.25%, 0.5%, and 1% mepivacaine; and 0.125%, 0.25%, and 0.5% ropivacaine. RESULTS Viability of chondrocytes was significantly decreased with exposure to 0.25% bupivacaine, 1% lidocaine, 1% LP, 1% mepivacaine, and 0.25% ropivacaine. Viability of FLSs was significantly decreased with exposure to 0.25% bupivacaine, 1% mepivacaine, 1% LP, and 0.5% ropivacaine. CONCLUSIONS AND CLINICAL RELEVANCE Clinically relevant concentrations of LAs had in vitro time- and concentration-dependent cytotoxicity for chondrocytes and FLSs isolated from the metacarpophalangeal joints of healthy horses. Bupivacaine was more toxic to chondrocytes than lidocaine, mepivacaine, and ropivacaine, whereas bupivacaine, LP, mepivacaine, and ropivacaine were more toxic to FLSs than preservative-free lidocaine. Several LAs may negatively affect chondrocyte and FLS viability.

Dogs with lower airway pathology that present in respiratory distress often receive oxygen therapy as the first line of treatment regardless of the underlying cause. Conventional "low-flow" systems deliver oxygen with a maximum flow rate of 15 L/minute. Traditionally, when an animal's respiratory status does not improve with conventional oxygen therapy and treatments for underlying disease, options might be limited to either intubation and mechanical ventilation or humane euthanasia. High-flow oxygen therapy (HFOT) has been gaining popularity in veterinary medicine as an alternative route of oxygen supplementation for animals that require support beyond conventional therapy. High-flow oxygen therapy can supply a mixture of air and oxygen via a heated and humidified circuit. It is user friendly and can be used in an environment in which mechanical ventilation is unavailable. This review article is written for emergency doctors and general practitioners who lack access to mechanical ventilation. This article briefly reviews pertinent respiratory physiology, traditional oxygen supplementation techniques, the physiology of HFOT, and the limited evidence available in veterinary medicine regarding the use of HFOT, its applications, and limitations. Guidelines for the use of HFOT are suggested and HFOT is compared to conventional therapy.

Since June 2017, several outbreaks of a Seneca Valley virus (SVV) USA/GBI29/2015-like strain have emerged in pigs in China. In our study, we successfully isolated the SVV strain CH-GDZQ-2018, confirmed by immunofluorescence and Western blot assays. Phylogenetic and recombinant analyses showed that the USA/GBI29/2015-like CH-GDZQ-2018 strain was the result of recombination between epidemic strains local to Guangdong, showing that SVV has undergone evolution in China.

The objective of this retrospective study was to evaluate the expression of receptor tyrosine kinases (RTKs) in canine adrenal tumors and correlate this expression with features of tumor aggressiveness and survival in dogs undergoing adrenalectomy. Forty-three canine adrenal tumors were evaluated for expression of c-kit, fms-like tyrosine kinase 3 (flt-3), platelet-derived growth factor receptor-β (PDGFR-β), and vascular endothelial growth factor receptor 2 (VEGFR2) using immunohistochemistry. Tumor RTK staining characteristics were compared to normal adrenals. Medical records were reviewed for data regarding patient outcome and tumor characteristics. Expression of c-kit, flt-3, PDGFR-β, and VEGFR2 was detected in 26.9%, 92.3%, 96.2%, and 61.5% of cortical tumors and 0%, 63.2%, 47.4%, and 15.8% of pheochromocytomas, respectively. Expression of RTKs was not significantly increased when compared to normal adrenals and did not correlate with survival after adrenalectomy. Receptor tyrosine kinases are not overexpressed in canine adrenal tumors compared to normal adrenal tissue. Therapeutic inhibition of these receptors may still represent an effective approach in cases where receptor activation is present.

OBJECTIVE To compare efficacy and duration of desensitization of oral structures with a lidocaine-bupivacaine mixture administered via a lateral percutaneous or modified infraorbital approach. ANIMALS 6 healthy adult hound-type female dogs. PROCEDURESIn this crossover study, dogs were randomized for side (left or right) and maxillary nerve approach (lateral percutaneous or infraorbital), with a 2-week washout period. Dogs were anesthetized, and a 2-mL mixture of 2% lidocaine and 0.5% bupivacaine (50:50 [vol/vol]) was administered with a 22-gauge, 4.5-cm-long catheter inserted through the infraorbital canal (infraorbital approach) or with a shielded stimulating needle to the maxillary nerve (percutaneous approach). Reflex-evoked motor potentials were measured for the maxillary canine tooth, fourth premolar tooth, second molar tooth, and hard palate mucosa ipsilateral to the injected mixture and for the contralateral maxillary canine tooth (control) at three 10-minute intervals before injection (baseline) and at predetermined times after injection for up to 6.7 hours. For each oral structure, the proportion of dogs with desensitization (efficacy) and time to onset and duration of desensitization were compared between approaches. RESULTS The proportion of dogs with successful nerve blockade did not significantly differ between infraorbital and percutaneous approaches and among the 4 oral structures. Time to onset of desensitization did not differ between approaches, but duration was significantly longer with the infraorbital approach. CONCLUSIONS AND CLINICAL RELEVANCE A modified infraorbital approach with the lidocaine-bupivacaine mixture had similar effects to a lateral percutaneous approach but provided a longer duration of desensitization. Neither approach was universally successful at desensitizing all oral structures.

Enzootic bovine leukosis (EBL) is a notifiable infectious disease with sporadic fre-quency in Albania. EBL is not a zoonotic disease, but it has an important impact on the cattle trade. Diagnosis of this disease can be performed by many diagnostic proce-dures, including agar gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) tests. In some cases, where persis-tent lymphocytosis is present, cell count can turn useful. This study aimed to evaluate serological and hematological tests\' suitability for the diagnosis of EBL and assess the manual and automatic hematological count technique diagnostic parameters com-pared to the serological test. Results suggest that strategic use of hematological and AGID tests in parallel can increase the sensitivity of diagnosis and facilitate the detec-tion of infected animals in different disease stages. Moreover, our results indicate that this approach is feasible in small scale cattle herds size, which properly fits Albanian circumstances

Lighting is a complex management tool in turkey production, controlled by three parameters; daylength, intensity, and chromaticity. As light-emitting diodes (LED) increase in popularity as alternatives to traditional light sources, research regarding LED impacts on commercial-type turkey production is lacking. Therefore, turkey hens of the same strain were reared under experimental brooding and grow-out conditions with six lighting treatments. An environmentally and light controlled facility (ECF) consisted of 5,000 Kelvin (K) LED or 5,000K + far-red LED (639nm) (RED) with either 12h short or 18h long daylength to test LED spectra. In the remaining treatments, hens were exposed to sunlight in a curtain-sided facility (CSF) as two treatments 1) natural decreasing daylength from September to November (NAT) or 2) natural daylight + 5,000 K LED lighting with an 18h long blocked daylength (BLK). The intensity was 9 footcandles in the ECF and naturally fluctuating in the CSF. Hen\'s performance was evaluated at 5, 9, and 14 weeks for eye development and feather coverage. Hens brooded and grown-out under NAT light had significantly increased body weight gain compared to BLK hens for the same period. Hens reared with RED LED spectrum had significantly increased eye anterior-posterior distance than birds raised in the NAT treatment. A significant reduction in red heat signature on the breast tissue in the NAT treatment compared to all other 18h treatments indicated improved feather coverage. However, these same results were not observed during serum thyroid hormone analysis. While turkey hens reared under different lighting programs had similar ending performance, lighting parameters significantly affected bird performance during the growing period, bird\'s eye development, and body feather coverage. Therefore, potential effects on growth patterns and physiology should be considered when choosing a LED lighting program for turkeys.

Histomoniasis is a parasitic disease of poultry with worldwide prevalence, having significant negative impacts on infected turkey health and welfare. A total of 53,000 male fattening turkeys (British United Turkeys, BUT6) of 140-day-old were admitted to the slaughterhouse in central Ukraine. No clinical signs were reported in the turkeys during the production cycle. Slaughtering of the turkeys revealed enlargement of the livers, most of which had a greenish color with alterations in the hepatic tissues (fibrosis). No other gross lesions were noted in other organs of the slaughtered birds. Fresh liver tissues were submitted to the laboratory to measure the concentrations of lead, cadmium, copper, and zinc to detect possible intoxication and/or differential diagnosis. The concentrations of the measured heavy metals in the affected liver tissues were within the recommended normal ranges. Histological examination revealed microscopically proliferative changes in liver sections in different turkeys. Suspected tissue sections were stained with Periodic Acid Schiff and found positive for Histomonas meleagridis trophozoites. Overall, macroscopical or microscopical examinations of turkey livers are essential to confirm infection with histomonads, even if the clinical signs are unapparent. As outbreaks of histomoniasis in turkey flocks are increasing approaches to prevent and treat histomoniasis are urgently needed.

Lumpy skin disease (LSD) is an infectious economically important viral disease of cattle. Lumpy skin disease virus (LSDV) is still circulating in Egypt, despite the annual mass vaccination with sheep pox virus vaccine by the Egyptian authorities. This study was carried out on 2 farms of pregnant Holstein cows vaccinated with Bovivax LSD-N® Vaccine (farm I) and Servac Capri-C vaccine® (farm II). After 40-60 days post-vaccination, mild clinical sings were detected in 3% of farm I cows whereas a more severe clinical infection was developed in 40% of cows in farm II. LSDV was isolated on the chorioallantoic membrane (CAM) of 11 days old emberyonated chicken egg (ECEs) and Madin Darby bovine kidney (MDBK) tissue culture. LSDV was identified in collected skin tissues by Transmission electron microscopy (TEM) and Histopathological examination. Finally, LSDV was confirmed by polymerase chain reaction (PCR) amplification of 192 base pair (bp) of P32 gene and two samples were selected for DNA sequencing. LSDV developed characteristic pock lesion in inoculated ECEs. MDBK cell culture developed prominent LSDV cytopathic effect at the 3rd passage. Viral particles were detected in the cytoplasm of both epidermal cells and dermal macrophages by TEM. Histopathological examination revealed different lesions correlated with LSDV infection age. LSDV was confirmed in all tested samples by PCR. Our strains (Dakahlia- 2020-1 and Dakahlia- 2020-2) were closely related to other Egyptian LSDV strains on GenBank with 98.2%-100% identity. The present study proved conclusive evidence that live attenuated Sheep Poxvirus vaccine poorly protect Egyptian cattle against LSDV, while LSDV Neethling strain vaccine gave a promising and sufficient protection rate.

Poultry meat is an important source of animal protein in Bangladesh. Small-scale poultry production plays a crucial role in the socio-economic development and nutritional requirements of rural and peri-urban households and provides an income source for resource-poor areas. Therefore, focusing on small-scale poultry production remains important. However, improper management, disease outbreak, market volatility and many other factors are typically responsible for the sustainability of this rising prospect of small-scale poultry production potential. The recent pandemic coronavirus disease of 2019 (COVID-19) tremendously influenced the sustainability of the small-scale poultry sector. This review addressed Bangladeshi small-scale poultry production and its market value, identified issues, and suggested potential guidelines to sustain the small-scale sector