Lactobacillus plantarum IRQ12 was isolated from local pickle producing from Cucumis sativus submerged in 5% salt solution for one week far from sun light, selective media was used for isolation and activation process which was MRS broth and MRS agar, biochemical tests like catalase, nitrate reducing, productive gas, citrate consumption, produced ammonia from arginine, produced indole from tryptophan, fluid gelatin, ability of growth in 15, 45 °C, carbohydrates fermentation test, were carried out. DNA of bacteria was extracted by DNA extraction kit, identification by 16S rDNA gene. the local strain tolerance ability was investigated for different levels of pH 4, 3, 2 and 1.5 for 0, 1, 2 and 3 hours, log cfu/ml at pH 4 after 3 hours of treatment was 7.83 and at pH 3 the log cfu/ml decrease to 6.8 after 3 hours of treatment. The log cfu/ml was decreased to 5.76 and 5.05 at pH 2 and 1.5 respectively after 3 hours of treatment.

A total of twelve oviducts of indigenous geese were used in this study. In immature geese the four parts of oviduct were lined by simple cuboidal to low simple columnar epithelium.The mean height of cuboidal epithelium was 20±1µm, and 29±2µm for columnar epithelium and at this period the intra epithelial capillaries were not observe in all parts of oviduct. The mature and laying geese the epithelium lining has changed into ciliated pseudostratified columnar epithelium which its height has measured about58±3µm in isthmus and 60±1µm in magnum. The intraepithelial capillaries were appeared only in the isthmus and magnum regions and most of these capillaries in the isthmus have arranged between the basal cells at the basement membrane. The intra epithelial capillaries of magnum region were observed at the center of epithelium.

The study was designed to evaluate the analgesic effects of Medetomidine and Tramadol in rabbits, to detect the best dose (as onset and duration) for antinociceptive in this model, also evaluate the antinociceptive effect as sedative doses in these drugs as a mixture by using electrical stimulator. Administration of Medetomidine alone at 200 µgkg B.W. (I.M) and Tramadol alone at 2 mg kg B.W. (I.P) were the best doses for relief pain induced by electrical stimuli. There was increased in the voltage change for pain ( 15, 30, 45, 60, 75, 90 minutes) in comparison with control and other doses for each drug. Administration of Medetomidine at 50 µg kg B.W. (I.M) with Tramadol at 0.5 mg kg B.W. I.P) significantly referred to synergism of the antinociceptive effect which induced analgesia in 100 % of the rabbits in comparison with other groups for each drug alone (at the same analgesic doses) without any overt side effects and without differences in glucose, glutathione, ALT and AST level in animals. The data of this study demonstrated the mixture of Medetomidine and Tramadol at low doses (subanalgesic doses) had a typical synergistic effect (super-additive) for inducing good and safe analgesia as well as its skeletal muscle relaxation in rabbits.

The Providencia rettgeri (P.rettgeri) is an opportunistic pathogen of human and animals and has a clinical importance as a nosocomial pathogen causing infections in the urinary system after catheterization and in immunocompromised patients less than human feces, bile and sputum. To estimati the pathogenic role of P.rettgeri in mice post-infection intraperitonial infection (I/P). thirty mice from both sexes, aged 6-7 weeks and weight 25-30 gm. were infected I/P by a whole bacterial suspension of P.rettgeri 0.5 ml (containing 1x108 CFU/ml), the negative control group injected I/P with (0.5 ml) PBS,five mice were sacrificed after (24, 48hrs. and 4,7 and 14 days) post-inoculation, also (n=5) were considered as negative control group, for histopathological examination. The histopathological changes at 24,48hrs until 4 days; recorded mild to moderate acute inflammatory cells reaction, edema, fibrinous exudate. At 7&14 days there was also necrosis and focal aggregation of mononuclear cells. Strong pathogenic role of P.rettegri in induction acute inflammatory response in experimental lab mice.

The study was conducted at the animal house of the collage of Veterinary medicine university of Basrah, the objective of this study to know effect ketamine drug as anesthetic agent in rabbits and comparison between several types of general anesthetic systems in rabbits and study the combination with Diazepam and fentanyl , in this study used (32) adult domestic rabbits from both gender , the average of body weight (1000-1900) grams , the rout of administration by intramuscular , were divided equally and randomly into four groups (8) rabbit in each groups .We were studied the induction time, surgical anesthesia time and recovery time also we were studied some physiological parameter (heart rate, respiratory rate and body temperature), also we were recorded (Degree of Analgesia, Eyes Reflexes and Degree of Muscle Relaxation).We were recorded data of this parameter before I.M administration at (Zero) minute and after I.M administration depending the following time (5, 10, 20, 30, 40, 50, 60) minute, until response the rabbit to external stimuli.The biochemical test are (ASTand ALT).

This study was carried out to evaluate the anti-inflammatory effect of losartan in 18 female rats which were divided into 3 groups. Respiratory disease were experimentally induced by the intraperitoneal injection and spray inhalation of ovalbumin (OVA) in first and second group while third group left as negative control group. First group were treated with losartan orally at dose rate of 5 mg/kg body weight, on the other hand second group considered as positive control group. ELISA test were used to estimate the concentration of TNF-α, IL-4 in BALF and total IgE in serum samples. Total WBC, neutrophil, eosinophil and lymphocyte were counted in bronchoaleveolar lavage (BAL). First and second rat groups show signs of pulmonary disease. Losartan treated group showed significant (p

The study was designed to describe the morphological and histological characteristics of the adrenal gland in female Duck (Anasplatyrhynchos).Ten adrenal glands was used in the study collected from five adult female ducks.The morphological features of glands were described and histological sections were prepared from each sample. The study revealed that theducks have paired glands located in the abdominal cavity at the anterior end of the kidneys.The shape of right gland is semi-circular while, the left elongated or triangular in shape. The mean weight of right gland of duck about (0.39) gram while, the left was (0.41)gram. The mean values of long axis of left adrenal gland about (15.46) mm and the diameter of right gave mean values about (11.30) mm. The histological sections of the adrenal gland revealed in a division of gland into a subcapsular layer (SZ)-(peripheral zone) and central zone (CZ) or inner zone. The subscapular zone (SZ) consisted of groups of cells immediately below the capsule which forms irregularly arranged cords while, there are two types of cells in central zone of gland, the first type of cells, acidophilic cells and the second type of cells, basophilic cells called chromaffin cells.

Real-time polymerase chain reaction assay was used for detection of Clostridium perfringens toxins genes alpha (cpa )directly from the fecal samples of cattle and sheep.Fecal samples from 20 clinically healthy cattle and 20 clinically healthy sheep were collected randomly from different farms located in AL-Diwanyia city ,All the strains of Clostridium perfringens were analyzed by Real-Time PCR using specific primers for alpha (cpa)toxin genes ,its reveald that (cpa)toxin gene of Clostridium perfringens ,were detected in cattles in 8(40%) and 14 (70%) in sheep Real time positive respectively samples ,This results indicate that Clostridium perfringens alpha toxin producing strains were prevalent in sheep and cattles and its possibly play an important role in the diarrheal disease caused by Clostridium perfringens .

The aim of the present work was to study chronic Babesiosis in drought horses in Basrah ,Iraq. Sixteen drought local breed horses more than (5) years old of both sexes were used in this study, show signs of generalized debility and icteric mucous membranes .The study were carried out in Basrah ,Iraq .Five clinically healthy local breed horses were considered as controls .Results revealed that diseased drought horses show signs of complete anorexia , severe emaciation and evident loss of body weight with poor tolerance , icteric mucous membranes of eyes, bulging of supra orbital fossa , edema were detected around fetlock joint, dehydration ,hemoglobinuria with intermittent episodes of colicky signs, Moreover , on clinical examination, diseased horses show shallow rapid respiration and accelerated strong heart beats with normal body temperature ,Moreover diseased horses suffering from constipation or diarrhea with presence or absence of cecal borborygmi sounds. Statistically significant increase were encountered in, respiratory , heart rates, and capillary refilling time, Furthermore Babesia caballi were detected microscopically on blood smears Giemsa stained, as large paired or crescent-shaped parasites ,Moreover it were also seen as , single and double pyriform with obtuse or acute angle in side the erythrocytes, In addition hematological examination of diseased horses with Babesiosis caballi revealed anemia represented by significant decrease in total erythrocytes count ,hemoglobin concentration and packed cell volume which were reflected macrocytic normochromic type due to significant increase in mean corpuscular volume ,Furthermore no significant difference were encountered in total and differential leukocytes count between diseased animals and controls ,in addition results were indicated no significant difference in clotting factors indices .It haven been concluded that chronic Babesiosis infected horses results in obvious hemolytic anemia and sever debility of diseased drought horses

The aim of this study was to investigate the acute toxicity of Organophosphate(Malathion and Atrazine), and determined the accumulation of these pesticides in thewhole body of zebrafish, and some biochemical responses in its brain and muscle.The results showed that MAL and ATR could be rapidly accumulated in the fish bodyshortly after being exposed to acute concentrations of the toxicants and exhibited adose-dependent manner. This was also validated by the response of antioxidantdefense, superoxide dismutase and catalase, both exhibited significant increase inactivity. Acetylcholinesterase activity of zebrafish was also shown inhibited in adose-dependent manner.