A 16-year-old, 7.4 kg, castrated male Cocker Spaniel presenting anorexia and weight loss was referred due to a splenic mass, and total splenectomy was performed to resolve clinical signs. Following surgery, the dog developed mild hyperkalemia (6.27 mmol/L) without any clinical symptoms. Further investigations were conducted to determine the cause of hyperkalemia. The serum-plasma potassium difference was measured (1.05 mmol/L), and pseudohyperkalemia was diagnosed. The cause of pseudohyperkalemia was considered as thrombocytosis after splenectomy. The dog did not receive any specific treatment to lower blood potassium. To our knowledge, we report the first case of post-splenectomy pseudohyperkalemia in a dog.

A captive female capybara (Hydrochoerus hydrochaeris) of unknown age discharged a bloody mass from the vaginal region. A histopathology examination revealed the mass to be a reproductive leiomyosarcoma, and an ovariohysterectomy was performed. The histopathology examination confirmed that the excised tissue was a uterine leiomyosarcoma. The purpose of this report is to describe clinical history and histopathological diagnosis of leiomyosarcoma in capybaras. This report is novel because it describes the first diagnosis of uterine leiomyosarcoma in a capybara. Since clinical data about capybaras are rare, this case report will help to diagnosis and treat reproductive diseases of this species.

A 12-year-old castrated male Persian cat presented with abdominal distension and anorexia. The radiography revealed ascites, pleural effusion, and a cranioventral mediastinal mass. Ultrasonography showed a well-defined mass lesion within a thickened ileal segment with a transmural loss of layering and multiple hyperechoic nodules arising from the peritoneum. Computed tomography showed peritoneal thickening with contrast enhancement, a bulky heterogeneous ileal mass, and mesenteric involvement with diffuse nodules. An alimentary lymphoma was confirmed by fine needle aspiration of the small intestinal mass. This report describes the imaging features of lymphoma with peritoneal lymphomatosis in a cat.

We describe 7 cases of splenic smooth muscle tumors in dogs. Histopathological and immunohistochemical analysis revealed that 6 cases were diagnosed as splenic leiomyosarcoma and 1 case as leiomyoma. All dogs underwent splenectomy without chemotherapy, and one of them was euthanized 2 months after surgery because of hepatic metastasis. Of the remaining 6 dogs, 5 died in the postoperative period and only one dog survived > 4 years. The median survival of the 6 dogs was 16.9 months. To the best of our knowledge, this is the first detailed study on splenic smooth muscle tumors in dogs in Korea.

Incidences of major feline viral diseases provide basic information for preventing viral disease in cats. Despite the growing interest in feline viral diseases, sero-surveillances have been lacking. In this study, we analyzed the diagnoses of feline viral diseases and conducted a sero surveillance of feline panleukopenia virus (FPV), feline calicivirus (FCV), feline herpesvirus-1 (FHV-1), and feline infectious peritonitis virus (FIPV) in Korean cats. Of the 204 confirmed cases since 2015, the numbers of diagnoses for FPV, FIPV, FCV, feline influenza virus, and FHV-1 were 156, 32, 12, 3, and 1 case, respectively. In total, 200 sera, collected between 2019 and 2021, were screened for the presence of antibodies against FPV, 2 FCVs, FHV-1, and FIPV using a hemagglutination inhibition test and a virus-neutralizing assay (VNA). The overall seropositive rates in cats tested for FPV, the 2 FCVs, FHV-1, and FIPV were 92.5%. 42.0%, 37.0%, 52.0%, and 14.0%, respectively. A low correlation (r = 0.466) was detected between the VNA titers of 2 FCV strains. The highest incidence and seropositive rate of FPV reveal that FPV is circulating in Korean cats. The low r-value between 2 FCVs suggests that a new feline vaccine containing the 2 kinds of FCVs is required.

A 12-year-old intact male Dalmatian dog presented hyporexia and vomiting for 1 week. Blood analysis revealed increased liver enzyme activity. Histopathological examination of the liver confirmed chronic hepatitis with fibrosis and necrosis. Copper staining revealed marked copper accumulation (2,770 ppm; normal range, 200 to 400 ppm), prominent in the centrilobular region, and compatible with copper-associated chronic hepatitis. However, copper metabolism domain containing 1 (COMMD1) mutation predisposing to copper accumulation in the liver tissue was not identified. The dog received medications but died 1 month after first visit. This is the first case of primary copper-associated hepatitis without COMMD1 mutation in a Dalmatian dog in South Korea.

No abstract available.

This study was carried out on a total of 550 lactating animals; 310 and 240 cows and buffaloes, respectively which were examined for signs of clinical mastitis (swelling, hotness, redness, and apparent milk change) from different dairy farms and veterinary units located at El-Fayoum Governorate during the period from May 2017 to November 2017. Clinical examination proved that out of these animals, a total of 126 animals (87 cattle and 39 buffaloes) were found with clinical mastitis. Streptococcus species were recovered from 73 animals including; 29(39.7%) and 44(60.0%) cows and buffaloes, respectively. Furthermore, out of the 73 Streptococci isolates recovered from cows and buffaloes; there were 10(13.7%) and 15(20.5%) S. agalactiae, 5(6.8%) and 10(23.7%) S. dysgalactiae, 8 (10.6%) and 7 (13.7%) S. uberis, 3(4.1%) and 10(13.7 %) E. fecalis and 3(4.1%) and 2(2.7%) S. lactarius, respectively. Anti-microbial susceptibility testing showed that the highest resistance was recorded against penicillin, gentamicin, streptomycin, and doxycycline (100%). Conversely, the highest sensitivity was recorded against ceftriaxone, ciprofloxacin, and sulfamethoxazole-trimethoprim (100%). Biofilm formation capacity was phenotypically assessed on YESCA CR agar medium and showed that all examined S. agalactiae and S. dysgalactiae were strong biofilm producers, meanwhile, 78%, 50%, and 75% of S. uberis, S. lactarius, and E. fecalis were biofilm positive isolates respectively. Application of PCR technique revealed that enterotoxins producing genes; sed, seb were found in 20% and 80% of isolates, in order. Biofilm-associated genes; fnbA and icaA genes were detected in 90% and 70%, respectively. Resistance genes; mecA and blaZ, genes were possessed in 90% and 70% of isolates, respectively.

The study was conducted from September 2018 to April 2019, A total of 350chicken samples were examined from different areas of the Basrah province for thediagnosis of ectoparasites and 154 chickens were infected with ectoparasites. Fourspecies of lice were isolated, which were Menacanthus cornutus, Menacanthusstramineus, Menacanthus pallidulus, Menapon gallinae and one species of mitewhich was Dermanyssus gallinae. The experimental study was carried out toeliminate the ectoparasites where 24 chickens were purchased and placed in the fieldof the faculty of agriculture. The chickens were divided into 3 groups. First group wasthe control group and the second group was infected with ectoparasites and the thirdgroup was infected with ectoparasites which was sprayed with garlic extract at aconcentration of 10%. Ten days later, the blood parameters were measured for thethree groups where the blood parameters of the group treated with garlic extract wereclose to the blood parameters of the control groupand show significant different(P<0.05).INTRODUCTION

The present study aims to isolate and identify Escherichia coli from fecal samples of farmanimals and human, also, it aims to molecular detection of shigatoxin and intimin genes inisolates. A total of (264) fecal samples and swabs were collected from different parts of Basrahin the period extending from September 2018 to January 2019. These samples were composed of(85) samples from cows, (94) samples from humanand (85) samples from sheep. Differenttechniques were used in this study to detect the presence of E. coli; these techniques includedconventional microbiological assays and molecular techniques (amplification of uidA gene byusing polymerase chain reaction).The results of these techniques indicated 50 (18.9%) were E. coli from the tested samples.These isolates were subjected to PCR to detect Shiga toxins and intimin genes (stx1, stx2, andeaeA). The results of PCR confirmed all (50) isolates were harbor at least one virulence gene.Out of 50 isolates 20 (40%) carried stx2 gene alone, the percentages of the carrier were (66.7 %,41.7% and 23.5%) from human, sheep and cattle samples, respectively. The genes (stx1 andstx2) were detected together in 9/50 (18%), represent (52.9%) of cattle isolates. The intimin gene(eaeA) alone was detected in 2/50 (4%), represent (11.8%) of cattle isolates. (28%) of isolatesharbor (stx2 and eaeA) genes, the isolates belong to human and sheep isolates (33.3%) and(45.8%), respectively. Presence of the genes (stx1, stx2, and eaeA) were discovered in (10%)of isolates, (11.8%) of cattle and (12.5%) of sheep.  The isolates were resistant to ampicillin, tetracycline (92%, 74%), respectively. However, theisolates were susceptible to imipenem, gentamycin, chloramphenicol, ciprofloxacin, andcefotaxime with a ratio of 100%, 92%, 78%, 68%, and 58%, respectively.