This study aimed to find out how different stress conditions (like temperature and pH) affect Salmonella enterica biofilm formation. This was done by looking at the phenotypic and genotypic features of isolates. 12 Salmonella enterica Isolate from animals, and 13 Salmonella enterica Isolate from people were used. S. enterica isolates were grown in tryptic soy broth (TSB) at (37°C, 25°C, and 42°C), and at pH levels (7, 5, and 9). The results revealed that the percentage was 52% in the standard conditions (temperature 37ºC and pH 7) while, in another condition, observed in the same temperature (37 °C) but with pH differences (pH 5, pH 9). S. enterica, did not produce biofilm. As for the stability pH in the, pH 7 with a change in, the temperature at 25°C percentage, biofilm produce (44%) while in 42 °C (64%). The detection rates of genes, biofilm-related PCR was used to find BapA and CsgD, were 100%. Although the biofilm formation of the phenotype did not give 100% results, the genotype gave 100%, which indicates that the gene is present but not expressed. Based on the findings in this study provided valuable information on the biofilm formation of Salmonella isolated from animals and humans.

In this project, 270 broiler chickens one day old were used to demonstrate the effect of the addition of the amino acid cysteine ​​added to the diets of birds contaminated with aflatoxin B1 on the liver and kidneys. The experiment was divided into 9 equal groups; & each group had 30 birds with 3 replicates, and each replicate had 10 birds. The control group was without addition. As for the treated groups, cysteine, ​​and aflatoxin B1 were added to their diets at 40%, 80% & 160% cysteine, and aflatoxin B1 was added at 0 ml, 4 ml & 8 ml, respectively. The variables collected were liver & kidney histopathology, Alanine aminotransferase (ALT), and Aspartate aminotransferase (AST) levels. When adding cysteine to a bird's diet contains Aflatoxin B1 not observed in blood ALT amount. The histopathological examination showed fibrosis in the liver and degeneration and dilatation of cortical tubules in the kidney. The amount of AST in the blood was greater at 28 days of age, specifically in G2 (Cysteine 80%) &G3 (Cysteine 160%) at Aflatoxin B1 0 ml, which caused significant damage to the liver. The giving of cysteine 40, 80& 160% in birds' feed contaminated with AflatoxinB1 0, 4& 8ml, which is intake by birds, has harmful effects on the health of the liver.

The current investigation was done to study the characteristic anatomical features of the trachea in the swan geese (Anser cygnoides). For that purpose, the methods included using 10 birds (5 males 5 females) collected between October and March. The birds were euthanized, the trachea was collected, and features such as location, relationship, length, weight, and volume were reported. The results revealed that the trachea was located between (caudally) first tracheosyrnigeal cartilage border and (rostrally) in the caudal border of the cricoid cartilage of the larynx. The skeleton of the trachea and each ring of the tracheal cartilages included both broad and narrow regions, with the broad parts of adjacent rings overlapping the narrow parts of the adjacent rings. The trachea was joined to two muscles. Sternotracheolaryngeus muscles, also called sternotrachealis muscles, are a pair of large skeletal muscles securely attached to the trachea at the tenth ring of the distal half, cranial to the pessulus cartilage of the syrinx. They are easy to see, face forward, and come from the craniolateral process of the sternum.  This serves as the primary origin of the caudolateral and caudomedial extrinsic muscles of the larynx. This study clearly shows the characteristic features of the trachea of the swan geese that could be useful buildups for future studies that deal with different sciences related to this important bird.

The present study aimed to investigate the effects of nano-capsulated ivermectin on the liver and kidney function and oxidative status in mite infested-rabbits, compared to ivermectin. Additionally, the ivermectin residue profile in adipose tissue, liver, muscle, and kidney was evaluated. For this purpose, nano-capsulated ivermectin was prepared and characterized using high-resolution transmission electron microscopy (HRTEM) and cytotoxicity assay on Vero cells. To assess the effect of dose escalation of nano-capsulated ivermectin, one-hundred naturally mite-infested male rabbits were divided into four groups (G1-G4; n=25). Rabbits kept in G1 were left untreated (positive control), while rabbits kept in G2 and G3 received subcutaneously 200 and 400 μg/kg body weight ivermectin, respectively, at zero-day and repeated after two weeks of the first injection. Rabbits in G4 were treated with 200 μg/kg nano-capsulated ivermectin at zero day as a single dose. Additionally, twenty-five healthy male rabbits (G0) were used as a negative control. The efficacy was assessed based on clinical manifestations, liver and kidney function, and oxidative stress parameters. Ivermectin residues were measured in fat, liver, muscle, and kidney using high-performance liquid chromatography (HPLC). Results showed that the size of the nano-capsulated ivermectin was 35.4 nm with a narrow size distribution of 0.578 polydispersity indexes. A significant improvement in liver and kidney functions (P

Background: Bovine brucellosis is an important zoonotic disease in Albania. Both, Brucella abortus and B. melitensis have been isolated from ruminants. National control and eradication programs for brucellosis are applied in sheep and goat farms as well as on larger dairy cattle farms. A study was performed in beef cattle farms, to assess herds and within herd prevalence. The prevalence of bovine brucellosis in small dairy herds was unknown and its importance has been largely neglected. The current study aimed to assess the herd and within herd prevalence of bovine brucellosis in small bovine herds and to provide scientific evidence for establishing an evidence-based approach to control the disease in this subset of the population not previously included in the national eradication program. To achieve this objective, a statistical survey was designed and implemented in small dairy herds in the Lushnja district where samples from statistically selected herds were serologically tested in parallel with Rose Bengal Test (RBT), Fluorescence Polarization Assay (FPA) and Enzyme-Linked Immunosorbent Assay (ELISA). Methodology: In total, 120 dairy herds were randomly selected from a list of 1,955 registered herds: from these selected herds 368 blood samples were collected from all animals older than 12 months, and their sera were tested using RBT, FPA, and c-ELISA. Results: The test results revealed no positive or suspect cases. Based on these results, we are confident (P ≤ 0.05) that currently in the Lushja district, Brucella spp is not circulating in this subpopulation of cattle. This deduction is supported by analyses of the main risk factors, other epidemiological data and the perceptions of official and private veterinarians. Conclusion: This is the first structured survey of bovine brucellosis in small dairy herds in Albania. In conclusion, our study results and our findings show that the epidemiological status of bovine brucellosis in the Lushnja district is encouraging. A test and slaughter control program appears to be appropriate in smaller herds. The approach used in this pilot study could be extended to establish the prevalence of brucellosis in other districts, the result of which would establish the basis for rational control measures in the smaller herds of cattle.

An outbreak of brucellosis in a dairy mixed farm with a total of 508 animals at Fayoum governorate, North Upper Egypt was investigated. The present study showed that extensive animal farming was a potential risk factor for interspecies transmission of brucellosis. The seroprevalences of brucellosis using Rose Bengal Test (RBT) and Buffered Acidified Plate Antigen Test (BAPAT) were (9.5%) in cattle, (35%) in sheep, and (50%) in camels. The milk ring test (MRT) identified fewer positive cases than BAPAT and RBPT due to less sensitivity to detect low concentrations of antibodies in milk or due to fat clustering factors. Therefore, MRT cannot be used alone to get rid of the infection inside the farm. A total of 31 Brucella isolates were recovered from cows and sheep on the farm. Bacteriological examination and molecular confirmation of isolated Brucella species using AMOS-PCR confirmed that all isolates were typed as Brucella melitensis biovar 3. AMOS-PCR was a perfect method for rapid, sensitive, and accurate Brucella detection at the species level. The strategy of test and slaughter has been applied to eradicate brucellosis from the farm. The early release of the herd out of the quarantine should be avoided especially under unhygienic conditions and lack of controlled movement of animals. However, the animal population must be subjected to successive serological examinations for the exploration of animals that may be still incubating the disease. Despite 50% of male camels being seropositive, no clinical signs have been reported. Mixed breeding systems have to be avoided and the application of biosecurity practices as well fair compensation policy for owners should be implemented.

Brucellosis is a zoonotic disease that has serious animal welfare and economic consequences worldwide. In mammals, this stealthy intracellular pathogen causes abortion and infertility, and in humans, it produces a terrible febrile illness that can progress into a long-term condition with serious implications. The pathogenicity of brucellae is based on their ability to survive and replicate in host cells, which allows them to escape from the immune system. The gold standard test for diagnosis, which demands competence, is still isolation and identification. Advancements in diagnostic procedures and screening of recently infected animals are required to achieve effective control. Despite their drawbacks, the most widely used vaccine strains to protect against Brucella infection and relevant abortions in cattle are B. abortus strains S19 and RB51 and in small ruminants is B. melitensis Rev1. However, there are no safe vaccine candidates for humans. Therefore, it is critical needs to improve vaccine production using advanced techniques such as subunit vaccines that are both effective and safe. Studying the overview of the Brucella immune response mechanism and advances in the diagnosis procedures allow more understanding of effective control strategies. The current review provides an overview on the immune response and updates on the diagnosis and control of brucellosis based on published literature on different search engines

The current study aimed to evaluate the objectivity of the vertebral heart scale in the right, left, and generalized cardiomegaly. The correlation between vertebral heart scale and cardiomegaly was determined by conventional methods using a detailed evaluation form of vertebral heart scale and main stem bronchus spine distance measurements and radiographic findings. For this reason, a detailed radiographic evaluation form, including cardiac shape alterations, tracheal displacement, the ratio of the width of the heart to the thorax, and effusive findings due to cardiac failure, was compiled to provide gradation and detection of both-sided alterations in latero-lateral and dorso-ventral view. X-rays of 189 symptomatic dogs with cardiac disorders were included in the study and evaluated by experienced staff. X-ray findings were classified as left-right and both-sided enlargement and graded as mild, moderate, or severe enlargement. Using statistical analysis, the vertebral heart scale method was found to be correlated with findings of left and generalized enlargement, and a significant difference was found between the moderate and severe class (respectively P

An 8-year-old spayed female, mixed dog presented multiple, bloody exudative skin lesions on the bilateral flank which spread 1 week after mastectomy for treatment of mammary gland tumor (MGT). Multiple, ill-marginated, irregular, and heterogeneously thickened cutaneous and subcutaneous lesions and enlarged lymph nodes were identified in ultrasound and computed tomography. Histopathological examination confirmed adenocarcinoma with lymphatic invasion presumed to be metastatic MGT. Clinical signs improved after chemotherapy but died after 1 month. This study suggests that cutaneous metastasis be considered for differential diagnosis of cutaneous lesions in dogs with a history of MGT, although skin metastasis from MGT is rare.

This paper describes a fatal case of pneumonia in a 14-day-old dog caused by extraintestinal pathogenic Escherichia coli (ExPEC). The necropsy showed that almost all of left lobes of the lungs had dark-red consolidation. A histopathology examination revealed moderate acute fibrino-hemorrhagic necrotizing pneumonia with intralesional bacterial colonies. Non-suppurative epicarditis, congestion in the liver, and necrosis in the white pulp of the spleen also were found. E. coli with cytotoxic necrotizing factor 1 and α-hemolysin was isolated from the lung. This case was confirmed to have fatal pneumonia caused by ExPEC that led to a systemic infection.