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Enzyme-linked immunosorbent assay for canine serum ferritin, using monoclonal anti-canine ferritin immunoglobulin G.
1988
Weeks B.R. | Smith J.E. | Phillips R.M.
Immunoassay of serum ferritin is currently used to evaluate the clinical iron status of human beings, horses, cattle, and swine. Because ferritins are immunologically species specific, a separate assay must be developed for each species. We have developed an ELISA for serum ferritin in dogs, using a monoclonal anti-canine ferritin antibody. Ferritin standards were linear (r = 0.997) from 0 to 80 ng/ml. Recovery of ferritin from canine serum was 94%. Dilutions of pooled canine serum were linear from 0 to 50% (r = 0.994). Within-assay coefficient of variability was 5.5%, whereas assay-to-assay coefficient of variability ranged from 12.5 to 21%. This assay should provide a nonsurgical means of accurately estimating dogs' iron stores.
显示更多 [+] 显示较少 [-]Effects of infection with parainfluenza-3 virus and infectious bovine rhinotracheitis virus on neutrophil functions in calves.
1988
Briggs R.E. | Kehrli M. | Frank G.H.
Hypotension and cutaneous reactions associated with intravenous administration of etoposide in the dog.
1988
Ogilvie G.K. | Cockburn C.A. | Tranquilli W.J. | Reschke R.W. | Weigel R.M.
A study was undertaken to determine the pressor and toxic effects of etoposide, an antineoplastic agent, when administered IV in 0.9% sodium chloride solution (0.4 mg of etoposide/ml) over a 30-minute period to dogs at a dosage of 40 mg/m2 of body surface. On day 1, 6 adult German Shorthaired Pointers were anesthetized with halothane, and blood pressures were measured via a femoral artery catheter before, during, and after the etoposide was administered. Systolic, diastolic, and mean blood pressures of each dog increased significantly (P less than 0.01) within 30 minutes after initiation of etoposide infusion. On day 3, when the dogs were not anesthetized, etoposide was again administered to each dog, using the same dosage. Each dog developed a moderate to severe cutaneous reaction characterized by moderate to severe pruritus, urticaria, and swelling of the head and extremities that began during the second infusion of etoposide. These same cutaneous reactions were seen on day 30, when etoposide was administered to 3 of the previously treated dogs and 2 previously untreated Beagles. We concluded that the administration of the commercial preparation of etoposide is likely to cause a significant reduction in blood pressure of anesthetized dogs, and that the drug is likely to induce a moderate to severe cutaneous reaction when administered to unanesthetized dogs.
显示更多 [+] 显示较少 [-]Effect of transport on feeder calves.
1988
Cole N.A. | Camp T.H. | Rowe L.D. Jr. | Stevens D.G. | Hutcheson D.P.
Differential extraction of antigens of Anaplasma marginale.
1988
Adams J.H. | Smith R.D.
Induction and characterization of acrosome reaction in equine spermatozoa.
1987
Varner D.D. | Ward C.R. | Storey B.T. | Kenney R.M.
Use of viable-cell ELISA for detection of monoclonal antibodies recognizing tumor-associated antigens on bovine lymphosarcoma cells.
1987
Aida Y. | Onuma M. | Kasai N. | Izawa H.
Neurophysiologic maps of cutaneous innervation of the hind limb of sheep.
1987
Kirk E.J. | Kitchell R.L. | Johnson R.D.
Radioimmunoassay for parathyroid hormone in equids.
1987
Roussel A.J. | Lin Y.C. | Strait J.R. | Modransky P.D.
Clinical pathologic alterations in horses during a water deprivation test.
1987
Genetzky R.M. | Loparco F.V. | Ledet A.E.
