Sumatran elephant (Elephas maximus sumatranus) was one of five big mammals in Way Kambas National Park (WKNP). Cytogenetic study was a part of conservation effort for sumatran elephant, especially in conservation genetic field. Captive sumatran elephants blood in Elephant Training Center (ETC) WKNP was collected to karyotype analysis. Captive sumatran elephants chromosome was analyzed by squashing technique with pretreating cells in a hypotonic solution. The study of karyotypes were made by giemsa staining applied to captive elephants blood cell. The diploid number of captive sumatran elephant was 56 both in male and female. The karyotype analysis performed different fundamental number in male and female, both of total fundamental number (FN) and autosomal fundamental number (FNa). Chromosome of female sumatran elephant showed the FN = 8, and FNa = 6, but in male sumatran elephant FN = 7, and FNa = . The karyotypes of X chromosome was large submetacentric and Y chromosome was small acrocentric.. The karyotype presented here will compilled with DNA fingerprinting analysis of all captive sumatran elephant in ETC WKNP as references in future breeding policy.

Sumatran elephant (Elephas maximus sumatranus) was one of five big mammals in Way Kambas National Park (WKNP). Cytogenetic study was a part of conservation effort for sumatran elephant, especially in conservation genetic field. Captive sumatran elephant’s blood in Elephant Training Center (ETC) WKNP was collected to karyotype analysis. Captive sumatran elephant’s chromosome was analyzed by squashing technique with pretreating cells in a hypotonic solution. The study of karyotypes were made by giemsa staining applied to captive elephant’s blood cell. The diploid number of captive sumatran elephant was 56 both in male and female. The karyotype analysis performed different fundamental number in male and female, both of total fundamental number (FN) and autosomal fundamental number (FNa). Chromosome of female sumatran elephant showed the FN = 8, and FNa = 6, but in male sumatran elephant FN = 7, and FNa = . The karyotypes of X chromosome was large submetacentric and Y chromosome was small acrocentric.. The karyotype presented here will compilled with DNA fingerprinting analysis of all captive sumatran elephant in ETC WKNP as references in future breeding policy.

Although urban spaces are increasingly recognised as viable habitats for wildlife, cities remain a major source of anthropogenic mortality for wild birds. While the sources of urban avian mortalities have been well documented in North America, these phenomena remain poorly studied in Southeast Asia, especially for resident species. Here we present the first summary of non-migratory urban bird mortalities for the heavily urbanised island of Singapore. We conducted a citizen science study using print and social media outreach to encourage members of the public to report their observations of dead birds between November 2013 and October 2017, and collected a total of 362 mortality records across 65 resident bird species and five mortality sources. Our results show that a diverse array of bird species is directly impacted by anthropogenic sources of mortality, although mortalities stemming from roadkill and cat predation are likely to be undersampled. We also find that forest-edge frugivores such as the Pink-necked Green Pigeon are likely to be especially vulnerable to building collisions. Our study shows that despite its limitations, opportunistic sampling using citizen science can generate large amounts of ecological data at relatively low cost, and serve as a cost-effective complement to standardised survey methodologies.

Although urban spaces are increasingly recognised as viable habitats for wildlife, cities remain a major source of anthropogenic mortality for wild birds. While the sources of urban avian mortalities have been well documented in North America, these phenomena remain poorly studied in Southeast Asia, especially for resident species. Here we present the first summary of non-migratory urban bird mortalities for the heavily urbanised island of Singapore. We conducted a citizen science study using print and social media outreach to encourage members of the public to report their observations of dead birds between November 2013 and October 2017, and collected a total of 362 mortality records across 65 resident bird species and five mortality sources. Our results show that a diverse array of bird species is directly impacted by anthropogenic sources of mortality, although mortalities stemming from roadkill and cat predation are likely to be undersampled. We also find that forest-edge frugivores such as the Pink-necked Green Pigeon are likely to be especially vulnerable to building collisions. Our study shows that despite its limitations, opportunistic sampling using citizen science can generate large amounts of ecological data at relatively low cost, and serve as a cost-effective complement to standardised survey methodologies.

The study was conducted in the village Payangkalang Parangloe District of Gowa in South Sulawesi in 2017, with the field survey method Substance himself to Bali beef cattle breeders, through interviews and questionnaires to 18 farmers, by the way (sample random sampling). Respondents beef cattle breeders, classified into 2 (two) business model with a scale of 2 adult males and 3 females scale adult stem, this data will be analyzed using economic analysis of income B/C. Based on the information that the beef cattle population of Bali is quite a many, so that the criterion for consideration for the study area. Beside that all cattle breeders Bali is already a member of a group of farmers. The results showed that net profit in the maintenance of Bali beef cattle female parent of $13.381.250/year with B/C ratio of 1.2 and a gain on the maintenance of male cattle Bali for $ 3.881.250 year, with B/C ratio of 1.1. To maintain a profitable cattle faremr cows, while the majority of beef cattle Bali parent can still be maintained as an investment for the procreation of children next year.

The study was conducted in the village Payangkalang Parangloe District of Gowa in South Sulawesi in 2017, with the field survey method Substance himself to Bali beef cattle breeders, through interviews and questionnaires to 18 farmers, by the way (sample random sampling). Respondents beef cattle breeders, classified into 2 (two) business model with a scale of 2 adult males and 3 females scale adult stem, this data will be analyzed using economic analysis of income B/C. Based on the information that the beef cattle population of Bali is quite a many, so that the criterion for consideration for the study area. Beside that all cattle breeders Bali is already a member of a group of farmers. The results showed that net profit in the maintenance of Bali beef cattle female parent of $13.381.250/year with  B/C ratio of 1.2 and a gain on the maintenance of male cattle Bali for $ 3.881.250 year, with B/C ratio of 1.1. To maintain a profitable cattle faremr cows, while the majority of beef cattle Bali parent can still be maintained as an investment for the procreation of children next year.

This study was aimed to determine the effect of epididymis extract (EE) on the testosterone and dihydrotestosterone (DHT) level of local male goat. An experimental study was performed using a completely randomized design (CRD) pattern of one-way analysis of variance (ANOVA). 15 local male goats aged 1.5 years with body weight 14-16 kg were used in this study. The K0 group as a control group, injected with only 1 ml physiological saline, while each KP1, KP2, KP3, and KP4 groups treated with multilevel EE dose, ie 1, 2, 3, and 4 ml / goat for 13 consecutive days. At the end of treatment (day 14th), testes, epididymis (caput, corpus, and cauda) and ductus deferens samples were taken through the close-castration method for examining the testosterone and DHT concentration by using enzyme-linked immunosorbent assay (ELISA) technique. Data gathered were later analyzed using ANOVA followed by Tukeys HSD in SPSS 16.0 for Windows. The result showed that the average concentration of testosterone on K0, KP1, KP2, KP3, and KP 4 in testis respectively were 10.002.64 ng/ml; 7.662.51 ng/ml; 10.006.55 ng/ml; 0.660.57 ng/ml; 11.667.37 ng/ml; caput epididymis; 5.001.73 ng/ml; 2.331.52 ng/ml; 5.002.64 ng/ml; 1.330.57 ng/ml; 5.661.15 ng/ml; corpus epididymis; 1.330.57 ng/ml; 0.660.57 ng/ml; 4.002.64 ng/ml; 0.660.57 ng/ml; 4.332.30 ng/ml; cauda epididymis: 1.000.00 ng/ml; 0.660.57 ng/ml; 1.660.57 ng/ml; 1.00 0.00 ng/ml; 2.001.73 ng/ml; ductus deferens: 3.662.51 ng/ml; 0.660.57 ng/ml; 3.001.00 ng/ml; 1.000.00 ng/ml and 3.661.15 ng/ml. While the average concentration of DHT on K0, KP1, KP2, KP3, and KP 4 in testis respectively; 10.002.64 ng/ml; 7.662.51 ng/ml; 10.006.55 ng/ml; 0.660.57 ng/ml; 11.667.37 ng/ml; caput epididymis; 5.001.73 ng/ml; 2.331.52 ng/ml; 5.002.64 ng/ml; 1.330.57 ng/ml; 5.661.15 ng/ml; corpus epididymis; 1.330.57 ng/ml; 0.660.57 ng/ml; 4.002.64 ng/ml; 0.660.57 ng/ml; 4.332.30 ng/ml; cauda epididymis: 1.000.00 ng/ml; 0.660.57 ng/ml; 1.660.57 ng/ml; 1.00 0.00 ng/ml; 2.001.73 ng/ml; ductus deferens: 3.662.51 ng/ml; 0.660.57 ng/ml; 3.001.00 ng/ml; 1.000.00 ng/ml and 3.661.15 ng/ml. Statistical analysis showed that the administration of EE only increased testosterone concentration in testes had significant effect (P 0.05). From this study, it can be concluded that the EE has the potential to improve spermatogenesis and sperm quality through increasing the testosterone concentration in the local male goats.

This study was aimed to determine the effect of epididymis extract (EE) on the testosterone and dihydrotestosterone (DHT) level of local male goat. An experimental study was performed using a completely randomized design (CRD) pattern of one-way analysis of variance (ANOVA). 15 local male goats aged 1.5 years with body weight 14-16 kg were used in this study. The K0 group as a control group, injected with only 1 ml physiological saline, while each KP1, KP2, KP3, and KP4 groups treated with multilevel EE dose, ie 1, 2, 3, and 4 ml / goat for 13 consecutive days. At the end of treatment (day 14th), testes, epididymis (caput, corpus, and cauda) and ductus deferens samples were taken through the close-castration method for examining the testosterone and DHT concentration by using enzyme-linked immunosorbent assay (ELISA) technique. Data gathered were later analyzed using ANOVA followed by Tukey’s HSD in SPSS 16.0 for Windows. The result showed that the average concentration of testosterone on K0, KP1, KP2, KP3, and KP 4 in testis respectively were 10.00±2.64 ng/ml; 7.66±2.51 ng/ml; 10.00±6.55 ng/ml; 0.66±0.57 ng/ml; 11.66±7.37 ng/ml; caput epididymis; 5.00±1.73 ng/ml; 2.33±1.52 ng/ml; 5.00±2.64 ng/ml; 1.33±0.57 ng/ml; 5.66±1.15 ng/ml; corpus epididymis; 1.33±0.57 ng/ml; 0.66±0.57 ng/ml; 4.00±2.64 ng/ml; 0.66±0.57 ng/ml; 4.33±2.30 ng/ml; cauda epididymis: 1.00±0.00 ng/ml; 0.66±0.57 ng/ml; 1.66±0.57 ng/ml; 1.00 ± 0.00 ng/ml; 2.00±1.73 ng/ml; ductus deferens: 3.66±2.51 ng/ml; 0.66±0.57 ng/ml; 3.00±1.00 ng/ml; 1.00±0.00 ng/ml and 3.66±1.15 ng/ml. While the average concentration of DHT on K0, KP1, KP2, KP3, and KP 4 in testis respectively; 10.00±2.64 ng/ml; 7.66±2.51 ng/ml; 10.00±6.55 ng/ml; 0.66±0.57 ng/ml; 11.66±7.37 ng/ml; caput epididymis; 5.00±1.73 ng/ml; 2.33±1.52 ng/ml; 5.00±2.64 ng/ml; 1.33±0.57 ng/ml; 5.66±1.15 ng/ml; corpus epididymis; 1.33±0.57 ng/ml; 0.66±0.57 ng/ml; 4.00±2.64 ng/ml; 0.66±0.57 ng/ml; 4.33±2.30 ng/ml; cauda epididymis: 1.00±0.00 ng/ml; 0.66±0.57 ng/ml; 1.66±0.57 ng/ml; 1.00 ± 0.00 ng/ml; 2.00±1.73 ng/ml; ductus deferens: 3.66±2.51 ng/ml; 0.66±0.57 ng/ml; 3.00±1.00 ng/ml; 1.00±0.00 ng/ml and 3.66±1.15 ng/ml. Statistical analysis showed that the administration of EE only increased testosterone concentration in testes had significant effect (P 0.05). From this study, it can be concluded that the EE has the potential to improve spermatogenesis and sperm quality through increasing the testosterone concentration in the local male goats.