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Assessing tropism and genetic traits of carp oedema virus isolates to enhance detection strategies 全文
2024
Adamkowska, Natalia | Kiełpińska, Jolanta | Bergmann, Sven Michael
Assessing tropism and genetic traits of carp oedema virus isolates to enhance detection strategies 全文
2024
Adamkowska, Natalia | Kiełpińska, Jolanta | Bergmann, Sven Michael
Introduction Carp oedema virus (CEV) is a relatively understudied poxvirus. It exhibits an affinity for gill and skin epithelial cells. Investigations were conducted into selected aspects of CEV biology, with a focus on determining cell and tissue tropism of CEV, acquiring gene sequences and updating CEV tests in fish tissues. Material and Methods A total of 238 common carp tissue samples from nine aquaculture farms were analysed. The study evaluated the efficacy of intermediate detection of CEV by real-time PCR and in situ hybridisation. The genes encoding protein P4a were sequenced, analysed and aligned in a phylogenetic tree using a molecular evolution model. Results In situ hybridisation revealed the necessity to validate the Centre for Environment, Fisheries and Aquaculture Science protocols for sampling for CEV detection and to use the tissues for which the virus has the highest tropism, namely the skin and kidneys, rather than solely the gills. The level of genetic variability was determined, and it was shown that CEV mutates systematically. The creation of two distinct phylogenetic clades confirms certain strains’ description as Polish isolates. Conclusion Determining the localisation of CEV genetic material in organs and tissues is pivotal for shaping the World Organisation for Animal Health guidelines. The utility of molecular diagnostics has been demonstrated in the skin and kidney of carp, in addition to the gills, impelling their inclusion in diagnostic protocols. The clusters identified in the phylogenetic tree offer valuable insights for developing the current PCR primers. The prevalence of CEV infection in aquaculture, juxtaposed with its notably lower detection in wild fish, underscores the significance of mandatory molecular diagnostic testing for CEV in carp farming.
显示更多 [+] 显示较少 [-]Assessing tropism and genetic traits of carp oedema virus isolates to enhance detection strategies 全文
2024
Adamkowska Natalia | Kiełpińska Jolanta | Bergmann Sven Michael
Carp oedema virus (CEV) is a relatively understudied poxvirus. It exhibits an affinity for gill and skin epithelial cells. Investigations were conducted into selected aspects of CEV biology, with a focus on determining cell and tissue tropism of CEV, acquiring gene sequences and updating CEV tests in fish tissues.
显示更多 [+] 显示较少 [-]The regulatory action of acetylcholine and its receptors on B4 and C4 leukotriene formation in the porcine endometrium after experimental inflammogenic Escherichia coli infection 全文
2024
Jana Barbara | Całka Jarosław | Bulc Michał | Kawka Dominika
Endometritis is a very common pathology in animals which changes endometrial leukotriene (LT) formation and muscarinic 2 and 3 receptor subtypes (M2R/M3R) and α-7 nicotinic acetylcholine (ACh) receptor (α-7 nAChR) expression patterns. With the relationship between ACh, its receptors and LT production remaining unclear, the role of M2R, M3R and α-7 nAChR in action of ACh on the 5-lipoxygenase (5-LO), LTA4 hydrolase (LTAH) and LTC4 synthase (LTCS) protein abundances in the inflamed porcine endometrium and on the tissue secretion of LTB4 and LTC4 were studied.
显示更多 [+] 显示较少 [-]The grey wolf (Canis lupus) as a host of Echinococcus multilocularis, E. granulosus s.l. and other helminths – a new zoonotic threat in Poland 全文
2024
Karamon Jacek | Samorek-Pieróg Małgorzata | Bilska-Zając Ewa | Korpysa-Dzirba Weronika | Sroka Jacek | Zdybel Jolanta | Cencek Tomasz
The aim of this study was to estimate the occurrence of Echinococcus spp. and other helminth infections in grey wolves in south-eastern Poland.
显示更多 [+] 显示较少 [-]Serological evidence of Erysipelothrix rhusiopathiae antibodies in wild boars (Sus scrofa) in Poland 全文
2024
Czyżewska-Dors Ewelina Barbara | Augustyniak Agata | Piekutowska-Nóżka Ewa | Jężak Joanna | Kowalczyk Ewelina | Jabłoński Artur
This study aimed to evaluate the seroprevalence of Erysipelothrix rhusiopathiae among wild boars inhabiting Poland.
显示更多 [+] 显示较少 [-]Whole genome sequencing and analysis of a goose-derived Mycoplasma gallisepticum in Guangdong Province, China 全文
2024
Zhou Yuanyuan | Song Shuti | Li Weihuo | Jia Yixin | Li Yangshuo | Liang Jingyi | Yao Zhaofeng | Zhang Nan
Mycoplasma gallisepticum (MG) infection is a primary cause of chronic respiratory disease in poultry, threatening the economic viability of China’s goose-farming industry. This study investigated the pathogenicity and drug resistance of an MG strain isolated from geese and whole-genome sequenced the strain.
显示更多 [+] 显示较少 [-]How does adulteration of wax foundation affect phenoloxidase and lysozyme activities as selected parameters of immunity in Apis mellifera? 全文
2024
Strachecka Aneta | Chęć Magdalena | Olszewski Krzysztof | Staniszewska Patrycja | Dziechciarz Piotr | Gagoś Mariusz
The adulteration of wax foundation is, for many reasons, a growing problem of modern beekeeping not only in Europe but also around the world. Wax foundation contaminated with stearin addition leads to a brood die-off, while paraffin addition negatively affects the strength of combs. It is tenable that such adulterated wax foundation reduces bees’ immunity. The aim of the study was to determine the activities of two bee immune enzymes, lysozyme and phenoloxidase, in the haemolymph of worker bees which had emerged from combs with wax foundations contaminated with stearin or paraffin.
显示更多 [+] 显示较少 [-]The effect of coffee husks used as pellet bedding material on the intestinal barrier, immune-related gene expression and microbiota composition in the broiler chicken caecum 全文
2024
Biesek Jakub | Dunisławska Aleksandra | Kozdruń Wojciech
Using coffee husks as waste material for bedding contributes to sustainable development. A sustainable choice of bedding has also, however, to be a safe choice for poultry. The study analysed immune-related gene expression in the intestinal mucosa and indicator bacteria in caecal content collected from broiler chickens bedded on material with coffee husk addition.
显示更多 [+] 显示较少 [-]The influence of probiotic administration on the phagocytic and oxidative burst activity of neutrophils and monocytes in the peripheral blood of dairy cows during different lactation periods 全文
2024
Brodzki Piotr | Gorzkoś Hubert | Marczuk Jan | Lisiecka Urszula | Junkuszew Andrzej | Krakowski Leszek | Szczubiał Marek | Brodzki Nikodem | Głodkowska Katarzyna
The objective of this bovine peripheral blood study was a comparative assessment of the phagocytic activity of neutrophils and monocytes and of the intracellular killing capacity of neutrophils from cows given no probiotic and from cows which were administered a probiotic consisting of Saccharomyces cerevisiae, Lactobacillus acidophilus, Lactobacillus plantarum and Rhodopseudomonas palustris. These activity types were compared during different lactation periods.
显示更多 [+] 显示较少 [-]Effect of the addition of different forms of rosemary (Rosmarinus officinalis L.) on the quality of vacuum-packed minced pork 全文
2024
Śmiecińska Katarzyna | Daszkiewicz Tomasz | Krajewska Agnieszka | Kubiak Dorota
Apart from their antioxidant activity, plant-derived bioactive compounds can also positively affect the quality of meat and meat products by improving their sensory and microbiological properties and preventing discolouration. The aim of this study was to determine how the addition of different forms of rosemary improved the quality of pork.
显示更多 [+] 显示较少 [-]Biomarkers of oxidative stress, biochemical changes, and the activity of lysosomal enzymes in the livers of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against yersiniosis before a Yersinia ruckeri challenge 全文
2024
Kurhaluk Natalia | Grudniewska Joanna | Pękala-Safińska Agnieszka | Pajdak-Czaus Joanna | Terech-Majewska Elżbieta | Platt-Samoraj Aleksandra | Tkaczenko Halina
This study aimed to evaluate biomarkers of oxidative stress (2-thiobarbituric acid reactive substances, aldehyde and ketone derivatives of oxidatively modified proteins and total antioxidant capacity), the activity of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase), that of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase and acid phosphatase) and changes in biochemical parameters (alanine aminotransferase, aspartate aminotransferase, de Ritis ratio, lactate dehydrogenase activity, lactate and pyruvate levels and their ratio) in the liver tissue of fish that were vaccinated against enteric redmouth disease and challenged with its causative agent, the bacterium Yersinia ruckeri.
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