It is well known that posterior capsule opacification (PCO), one of the most common late postoperative complications of cataract surgery, is mainly caused by proliferation and differentiation of remaining lens epithelial cells (LECs) on the posterior lens capsule. Many authors suggest that alterations induced by the pathophysiology of cataracts, its metabolism and the use of 0.1% trypan blue (TB) must cause some degree of cellular damage on these cells, wicht would help to prevent and/or reduce the incidence of PCO after cataract surgery in humans. Therefore, the aim of this study was to evaluate the expression of cell death markers on LECs of older dogs with diabetic and hypermature cataracts, after capsulorhexis, both using 0.1% TB. Twenty samples collected from 13 dogs of different breeds, with ages varying from 8 to 12 years-old, with diabetic and hypermature cataracts, which had been subjected to phacoemulsification surgery (Phaco) using 0.1% TB for staining were studied. Animals were classified as dogs with diabetic (DC) and hypermature cataracts (HC), and expression of molecular markers for apoptosis and autophagy (caspase-3 and beclin-1) on LECs were obtained by immunofluorescence technique. The expression of caspase-3 and beclin-1 was observed in every studied sample and did not differ between groups. In conclusion, our findings suggest that apoptosis and autophagy processes occur to LECs in older dogs presenting diabetic and hypermature cataracts after Phaco utilizing 0.1% TB. Our results may be helpful to future studies of PCO in post-phacoemulsification surgery patients.

A opacificação da cápsula posterior da lente do globo ocular é a complicação mais observada após a remoção da lente. Essa patologia é causada principalmente pela proliferação e diferenciação das células do epitélio anterior da lente em sua cápsula posterior. Muitos autores sugerem que alterações induzidas pelo metabolismo e/ou patofisiologia da catarata e o uso do corante de azul de tripan a 0,1% devam causar algum dano a essas células, o que supostamente ajudaria a prevenir e reduzir a incidência de tal complicação em humanos. Este trabalho avaliou a expressão de marcadores de morte celular no epitélio anterior da lente de cães idosos com catarata diabética e hipermadura, após capsulorrexe realizada com o emprego do azul de tripan a 0,1%. Foram estudadas vinte amostras colhidas de treze cães de diferentes raças, com idades variando de oito a doze anos, que apresentavam catarata diabética ou hipermadura e que foram submetidos à facoemulsificação utilizando corante de azul de tripan a 0,1%. Foram designados dois grupos: com catarata diabética (DC) e com catarata hipermadura (HC). A expressão molecular dos marcadores de morte celular por apoptose a autofagia (caspase-3 e beclina-1) no epitélio anterior da lente foi avaliada pela técnica de imunofluorescência. Observou-se que a expressão de caspase-3 e beclina-1 ocorreu em todas as amostras e não foi diferente entre os grupos. Os achados deste estudo sugerem que o processo de morte celular por apoptose e autofagia ocorre no epitélio anterior da lente de cães idosos com catarata diabética e hipermadura submetidos à facoemulsificação com o corante de azul de tripan a 0,1%. Este resultado pode ser útil para estudos futuros da opacidade da cápsula posterior da lente em cães submetidos à facoemulsificação. | It is well known that posterior capsule opacification (PCO), one of the most common late postoperative complications of cataract surgery, is mainly caused by proliferation and differentiation of remaining lens epithelial cells (LECs) on the posterior lens capsule. Many authors suggest that alterations induced by the pathophysiology of cataracts, its metabolism and the use of 0.1% trypan blue (TB) must cause some degree of cellular damage on these cells, wicht would help to prevent and/or reduce the incidence of PCO after cataract surgery in humans. Therefore, the aim of this study was to evaluate the expression of cell death markers on LECs of older dogs with diabetic and hypermature cataracts, after capsulorhexis, both using 0.1% TB. Twenty samples collected from 13 dogs of different breeds, with ages varying from 8 to 12 years-old, with diabetic and hypermature cataracts, which had been subjected to phacoemulsification surgery (Phaco) using 0.1% TB for staining were studied. Animals were classified as dogs with diabetic (DC) and hypermature cataracts (HC), and expression of molecular markers for apoptosis and autophagy (caspase-3 and beclin-1) on LECs were obtained by immunofluorescence technique. The expression of caspase-3 and beclin-1 was observed in every studied sample and did not differ between groups. In conclusion, our findings suggest that apoptosis and autophagy processes occur to LECs in older dogs presenting diabetic and hypermature cataracts after Phaco utilizing 0.1% TB. Our results may be helpful to future studies of PCO in post-phacoemulsification surgery patients.

One of the biggest contributors of air pollution is the production of coal fly ash from power plant industry. Fly ash has an immediate impact on human and animal health around the power plant. Many buffaloes in Nagan Raya usually grazing near the 110-megawatt steam power plant that using low rank coal. This action raises concerns about buffalo’s health due to exposure to fly ash. This study aimed to examine the relationship of knowledge, attitude and action of buffalo breeders to the impact of fly ash exposure on the buffaloes. This research used analytical survey with cross sectional design. This study was conducted in February 2018 with 30 respondents from four villages around Nagan Raya Power Plant to evaluate their knowledge, attitudes and actions by using questionnaires. The data were analyzed statistically with Chi Square Test with SPSS for Windows version 17.0. The results showed that there was no significant correlation between the knowledge of buffalo breeders and the impact of fly ash exposure (P 0.05), while the attitude and actions of buffalo breeders had a significant correlation with the impact of fly ash exposure (P 0.05). These results concluded that the buffalo breeders in the villages around the power plant did not realize the impact of fly ash arising from coal of power plant. It is expected that the associated institution would conduct a counseling about the impact of fly ash exposure as well as good management of buffaloes.

One of the biggest contributors of air pollution is the production of coal fly ash from power plant industry. Fly ash has an immediate impact on human and animal health around the power plant. Many buffaloes in Nagan Raya usually grazing near the 110-megawatt steam power plant that using low rank coal. This action raises concerns about buffalos health due to exposure to fly ash. This study aimed to examine the relationship of knowledge, attitude and action of buffalo breeders to the impact of fly ash exposure on the buffaloes. This research used analytical survey with cross sectional design. This study was conducted in February 2018 with 30 respondents from four villages around Nagan Raya Power Plant to evaluate their knowledge, attitudes and actions by using questionnaires. The data were analyzed statistically with Chi Square Test with SPSS for Windows version 17.0. The results showed that there was no significant correlation between the knowledge of buffalo breeders and the impact of fly ash exposure (P 0.05), while the attitude and actions of buffalo breeders had a significant correlation with the impact of fly ash exposure (P 0.05). These results concluded that the buffalo breeders in the villages around the power plant did not realize the impact of fly ash arising from coal of power plant. It is expected that the associated institution would conduct a counseling about the impact of fly ash exposure as well as good management of buffaloes.

The activity of the cellulase enzyme and the content of phytoestrogen (genistein) forming is determined during the fermentation of soybean waste with Aspergullus niger. The analysis is performed at fermentation incubation time of 0, 48, 96, 144 and 192 hours and treatment with addition of micro nutrient (V2) and without micro nutrient (V1). Data analysis on cellulase enzyme activity on phytoestrogen forming material is performed using T test. The average of cellulase content of fermented soybean waste increases from 0.032-0.139 IU / ml (V1) and 0.061-0.158 IU / ml (V2) after 48 hours into 0.238 IU / ml (V1) and 0.245 IU / ml (V2) after 192 hours The average of genestein levels in the fermented soybean waste increases as well from 0.101 - 0.573 mg / g (V1) and 0.114 - 0.587 mg / g (V2) after 48 hours and increase into 0.722 mg / g (V1) and 0.699 mg / g (V2) after 192 hours. Statistical analysis indicates that the treatment of V1 and V2 has an effect on the increase of cellulase enzyme content and genistein level (P 0.05). The correlation between enzyme activity of cellulase and genistein on soybean waste fermented with Aspergillus niger is significant (R2 = 0,949). The relationship between the enzyme performance and its formation material is running synergistic, cellulase enzyme activity and genistein level experience significant increase during the fermentation process.

The activity of the cellulase enzyme and the content of phytoestrogen (genistein) forming is determined during the fermentation of soybean waste with Aspergullus niger. The analysis is performed at fermentation incubation time of 0, 48, 96, 144 and 192 hours and treatment with addition of micro nutrient (V2) and without micro nutrient (V1). Data analysis on cellulase enzyme activity on phytoestrogen forming material is performed using T test. The average of cellulase content of fermented soybean waste increases from 0.032-0.139 IU / ml (V1) and 0.061-0.158 IU / ml (V2) after 48 hours into 0.238 IU / ml (V1) and 0.245 IU / ml (V2) after 192 hours The average of genestein levels in the fermented soybean waste increases as well from 0.101 - 0.573 mg / g (V1) and 0.114 - 0.587 mg / g (V2) after 48 hours and increase into 0.722 mg / g (V1) and 0.699 mg / g (V2) after 192 hours. Statistical analysis indicates that the treatment of V1 and V2 has an effect on the increase of cellulase enzyme content and genistein level (P 0.05). The correlation between enzyme activity of cellulase and genistein on soybean waste fermented with Aspergillus niger is significant (R2 = 0,949). The relationship between the enzyme performance and its formation material is running synergistic, cellulase enzyme activity and genistein level experience significant increase during the fermentation process.

Sumatran elephant (Elephas maximus sumatranus) was one of five big mammals in Way Kambas National Park (WKNP). Cytogenetic study was a part of conservation effort for sumatran elephant, especially in conservation genetic field. Captive sumatran elephant’s blood in Elephant Training Center (ETC) WKNP was collected to karyotype analysis. Captive sumatran elephant’s chromosome was analyzed by squashing technique with pretreating cells in a hypotonic solution. The study of karyotypes were made by giemsa staining applied to captive elephant’s blood cell. The diploid number of captive sumatran elephant was 56 both in male and female. The karyotype analysis performed different fundamental number in male and female, both of total fundamental number (FN) and autosomal fundamental number (FNa). Chromosome of female sumatran elephant showed the FN = 8, and FNa = 6, but in male sumatran elephant FN = 7, and FNa = . The karyotypes of X chromosome was large submetacentric and Y chromosome was small acrocentric.. The karyotype presented here will compilled with DNA fingerprinting analysis of all captive sumatran elephant in ETC WKNP as references in future breeding policy.

Sumatran elephant (Elephas maximus sumatranus) was one of five big mammals in Way Kambas National Park (WKNP). Cytogenetic study was a part of conservation effort for sumatran elephant, especially in conservation genetic field. Captive sumatran elephants blood in Elephant Training Center (ETC) WKNP was collected to karyotype analysis. Captive sumatran elephants chromosome was analyzed by squashing technique with pretreating cells in a hypotonic solution. The study of karyotypes were made by giemsa staining applied to captive elephants blood cell. The diploid number of captive sumatran elephant was 56 both in male and female. The karyotype analysis performed different fundamental number in male and female, both of total fundamental number (FN) and autosomal fundamental number (FNa). Chromosome of female sumatran elephant showed the FN = 8, and FNa = 6, but in male sumatran elephant FN = 7, and FNa = . The karyotypes of X chromosome was large submetacentric and Y chromosome was small acrocentric.. The karyotype presented here will compilled with DNA fingerprinting analysis of all captive sumatran elephant in ETC WKNP as references in future breeding policy.

On April 2016, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Bogor Agricultural University (VTH FKH IPB) received 4 cases of femoral fractures in cats caused by trauma. Two cats suffered oblique diaphysis fracture femoral, one cat suffered comminuted diaphysis fracture femoral, and one cat suffered epiphyseal fracture femoral (column femoral and trochanter major). All cats were treated by open surgery using intramedullary pins (Steinmann) and cerclage wires (Kirschner). Fracture recovery went without complication and all cats were able to move their feet one week after surgery.

On April 2016, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Bogor Agricultural University (VTH FKH IPB) received 4 cases of femoral fractures in cats caused by trauma. Two cats suffered oblique diaphysis fracture femoral, one cat suffered comminuted diaphysis fracture femoral, and one cat suffered epiphyseal fracture femoral (column femoral and trochanter major). All cats were treated by open surgery using intramedullary pins (Steinmann) and cerclage wires (Kirschner). Fracture recovery went without complication and all cats were able to move their feet one week after surgery.

Eurytrematosis is a common zoonotic disease has emerged as a major species causing veterinary disease and public health problem. This review examines recent advances in Eurytrema species, the life cycle, intermediate hosts required for completing the life cycle, and geographical distribution of Eurytrema species in the world, as well as several factors considered suitable to occur.

Eurytrematosis is a common zoonotic disease has emerged as a major species causing veterinary diseaseand public health problem. This review examines recent advances in Eurytrema species, the life cycle, intermediatehosts required for completing the life cycle, and geographical distribution of Eurytrema species in the world, as wellas several factors considered suitable to occur.

Vaginal discharges are commonly seen in the pediatric population, approximately more than 50% of all pediatric genital complaints. Normal vaginal secretion is usually thin, and clear to white with a variable amount, while malodorous, abnormal consistency of vaginal discharge accompanied by blood, pain, pruritus, or dysuria is usually pathologic. Many factors can cause vaginal discharge such as anatomy of genital area that more susceptible to inflammation and infection, numerous organisms that including those associated with the sexually transmitted diseases or child sexual abuse, also mechanical and chemical substances, as well as poor hygiene. Recurrent vaginal discharge can be very distressing to children, especially if associated with discomfort and the most common cause is vulvovaginitis. The management of vaginal discharge is based on underlying causes, either by initiating proper therapeutic or teaching the children about good hygiene.

Vaginal discharges are commonly seen in the pediatric population, approximately more than 50% of all pediatric genital complaints. Normal vaginal secretion is usually thin, and clear to white with a variable amount, while malodorous, abnormal consistency of vaginal discharge accompanied by blood, pain, pruritus, or dysuria is usually pathologic. Many factors can cause vaginal discharge such as anatomy of genital area that more susceptible to inflammation and infection, numerous organisms that including those associated with the sexually transmitted diseases or child sexual abuse, also mechanical and chemical substances, as well as poor hygiene. Recurrent vaginal discharge can be very distressing to children, especially if associated with discomfort and the most common cause is vulvovaginitis. The management of vaginal discharge is based on underlying causes, either by initiating proper therapeutic or teaching the children about good hygiene.

This study was conducted to validate the DRG Cortisol ELISA EIA-1887 Germany kit for measure the concentration of stress hormone metabolites (cortisol) from the feces and its correlation to the stressor factor in captive elephants in PKG and CRU of Aceh. These factors are location, diet and presence of livestock. There is no special treatment, observation based on the activity, behavior or natural condition of the animals. The sampling technique was non invasive, fresh dung samples of each (±20 gram) were collected from 25 elephants in CRU and PKG. Feces taken in the morning (before the animals are bathed) along with the observation of animal behavior. All samples were collected and stored at -200C until the analysis process. The validation test are analytic (parallelilmsm) and biological validation test. The analytic test result (paralillsm), showed that the sample curve was not parallel to the standard curve, but crossed the standard curve. While the results of biological validation test, DRG Cortisol ELISA EIA-1887 Germany kit can measure the concentration of cortisol hormone feces of Sumatran elephant and able to describe the difference of cortisol concentration relation to physiological events (stress vs non-stress). The mean values of cortisol metabolite concentrations from PKG Saree (Komplek PKG and Hutan Seunapet), Sampoiniet CRU, Cot Girek, Das Peusangan, Meulaboh and Aceh Timur were (577 ng/g and 400 ng/g), 435ng /g, 419ng /g, 517ng / g, 401ng/g and 425ng /g. The measurement results correlate with the physiological conditions and observed  factors.

This study was conducted to validate the DRG Cortisol ELISA EIA-1887 Germany kit for measure the concentration of stress hormone metabolites (cortisol) from the feces and its correlation to the stressor factor in captive elephants in PKG and CRU of Aceh. These factors are location, diet and presence of livestock. There is no special treatment, observation based on the activity, behavior or natural condition of the animals. The sampling technique was non invasive, fresh dung samples of each (20 gram) were collected from 25 elephants in CRU and PKG. Feces taken in the morning (before the animals are bathed) along with the observation of animal behavior. All samples were collected and stored at -200C until the analysis process. The validation test are analytic (parallelilmsm) and biological validation test. The analytic test result (paralillsm), showed that the sample curve was not parallel to the standard curve, but crossed the standard curve. While the results of biological validation test, DRG Cortisol ELISA EIA-1887 Germany kit can measure the concentration of cortisol hormone feces of Sumatran elephant and able to describe the difference of cortisol concentration relation to physiological events (stress vs non-stress). The mean values of cortisol metabolite concentrations from PKG Saree (Komplek PKG and Hutan Seunapet), Sampoiniet CRU, Cot Girek, Das Peusangan, Meulaboh and Aceh Timur were (577 ng/g and 400 ng/g), 435ng /g, 419ng /g, 517ng / g, 401ng/g and 425ng /g. The measurement results correlate with the physiological conditions and observed factors.

The increase in beef production is constrained by the slow growth of beef cattle population which is caused by the cattle breeding business that is considered less commercially profitable. The supplying of beef needs in a critical and effective manner is always increasing each year, so the price of beef is fluctuate. The various priority concept of the government's main program for the development of beef cattle is always done in every region in Indonesia. But the production of beef is still less and the government have to import the beef to fulfill the beef production. Therefore, this issues need necessary operational steps to provide more open space for some factors and leverage points in the supplying of beef needs. The purpose of this review is to examine the efforts to supply beef needs for the future in Indonesia critically. The high price of beef is not a mistake of the government. Basically, the government has tried to make the beef cattle population increased, to fulfill the consumer needs and the prices of beef is affordable for the customer. To cope with the higher beef prices, the government is make a policy of developing small, medium-sized, and big beef cattle breeding industries through seed cattle spreads on plantations of oil palm, rubber and cultivable fields for the development of beef cattle population. It is expected that in the coming year, beef cattle business can be oriented to agribusiness bussiness so that farmer's welfare will increase and can support Indonesia as the world food granary.

The increase in beef production is constrained by the slow growth of beef cattle population which is caused by the cattle breeding business that is considered less commercially profitable. The supplying of beef needs in a critical and effective manner is always increasing each year, so the price of beef is fluctuate. The various priority concept of the government's main program for the development of beef cattle is always done in every region in Indonesia. But the production of beef is still less and the government have to import the beef to fulfill the beef production. Therefore, this issues need necessary operational steps to provide more open space for some factors and leverage points in the supplying of beef needs. The purpose of this review is to examine the efforts to supply beef needs for the future in Indonesia critically. The high price of beef is not a mistake of the government. Basically, the government has tried to make the beef cattle population increased, to fulfill the consumer needs and the prices of beef is affordable for the customer. To cope with the higher beef prices, the government is make a policy of developing small, medium-sized, and big beef cattle breeding industries through seed cattle spreads on plantations of oil palm, rubber and cultivable fields for the development of beef cattle population. It is expected that in the coming year, beef cattle business can be oriented to agribusiness bussiness so that farmer's welfare will increase and can support Indonesia as the world food granary.

This study aims to dicover the economic loss caused by nematodiasis in local goat farmings around the animal primary health care (Puskeswan) in Batee Roo, Aceh Jaya. In this study, as many as 84 goats positive of nematodiasis were used as samples for cross-sectional approach. Nematodiasis goats were confirmed from clinical symptoms and fecal examination using centrifugation method. Economic loss was determined by measuring body weight of all the goats. The results showed that the goats in the local farms had more than 70% prevalence rate of nematodiasis, which caused economic loss around Rp. 34.300,-/male goat, and Rp.9.310,-/ female goat. Based on the goats population in Batee Roo in 2014, total economic loss from nematodiasis for male goats reached Rp. 19.012.000,- per year, and for female it was Rp. 9.012.000,- per year. From this data, it can be concluded that the economic loss from nematodiasis in male and female goats around Puskeswan Batee Roo, Aceh Jaya per year could reach Rp. 20.000.000.

This study aims to dicover the economic loss caused by nematodiasis in local goat farmings around the animal primary health care (Puskeswan) in Batee Roo, Aceh Jaya. In this study, as many as 84 goats positive of nematodiasis were used as samples for cross-sectional approach. Nematodiasis goats were confirmed from clinical symptoms and fecal examination using centrifugation method. Economic loss was determined by measuring body weight of all the goats. The results showed that the goats in the local farms had more than 70% prevalence rate of nematodiasis, which caused economic loss around Rp. 34.300,-/male goat, and Rp.9.310,-/ female goat. Based on the goats population in Batee Roo in 2014, total economic loss from nematodiasis for male goats reached Rp. 19.012.000,- per year, and for female it was Rp. 9.012.000,- per year. From this data, it can be concluded that the economic loss from nematodiasis in male and female goats around Puskeswan Batee Roo, Aceh Jaya per year could reach Rp. 20.000.000.