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Purpose. Assessing under in vitro conditions the degree of resistance to agents of bacterial diseases of tomato varieties which are included into the State Register of plant varieties suitable for dissemination in Ukraine in 2015. Defining integrated biochemical indices of the quality of tomato fruits with different resistance to agents of bacterial diseases. Methods. In the course of performance biotechnological methods were used to select callus cells with increased resistance to the agents of bacterial diseases, biochemical ones – determine qualitative and quantitative indicators of the tomato fruit quality, statistical ones – analyse experimental data. Results. Studied tomato varieties of Ukrainian breeding had different resistance to warm cells of agents of bacterial speck, bacterial black spot and to exopolysaccharides of bacterial canker agents. ‘Chaika’, ‘Klondaik’, ‘Zoreslav’, ‘Flandriia’, ‘Legin’, ‘Oberig’, ‘Atlasnyi’, ‘Gospodar’ and ‘Kimmeriiets’ tomato varieties were distinguished by high palatability traits and quality. Conclusions. It was found that tomato varieties ‘Chaika’, ‘Klondaik’ and ‘Zoreslav’ are resistant to bacterial canker, bacterial speck and bacterial black spot; ‘Flandriia’ and ‘Legin’ – to bacterial black spot, ‘Oberig’, ‘Atlasnyi’, ‘Gospodar’ and ‘Kimmeriiets’ – to bacterial speck.

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