Purpose. To analyse the qualitative and quantitative composition of volatile organic substances (VOS) in the petals of six species of dog-rose in the collection of M. M. Gryshko National Botanic Garden of the NAS of Ukraine for further bree­ding.Methods. Volatile organic substances were obtained by steam distillation. VOS was determined using gas chromato­graphy (Chromatograph Agilent Technologies 6890).Results. The qualitative and quantitative composition of volatile organic substances in the petals in such species as Rosa L. (R. roullettii HCh (Correvon), R. multiflora Thunb., R. pimpinellifolia L., R. canina L., R. centifolia L., R. rugosa Thunb.) was investigated. According to the results of the research, 105 VOS were discovered in the dog-rose petals, among which 11 were not identified. The dog-rose petals of identified VOC contained 16 components which share exceeds 5%, 31 – was ranging from 1% to 5%, 47 – was less than 1%. They belonged to different groups including monoterpenoids, sesquiterpenoids, sesquiterpene alcohols, saturated unbranched hydrocarbons etc. The largest number of components were identified in the petals of R. roulettii (49) and R. centifolia (45), while the number of identified components in species R. multiflora (33), R. rugosa (31) and R. canina (30) was relatively smaller. All studied dog-rose species were characterized by the presence of such saturated unbranched hydrocarbons as decan, tetradecane, pentadecane, hexadecane, heptadecane, octadecane, nanodecane, heneicosane, tricosane, tetracosane, pentacosane, heptocosane, hentriacontane. Triterpene hydrocarbon squalene is the important component of the VOS complex in dog-rose pen tals. b-phenylethyl alcohol to be one of the main components of the rose essential oils was found during investigation in the following four dog-rose species as R. centifolia (0.61%), R. pimpinellifolia (3.56%), R. rugosa (4.24%), and R. multiflora (5.43%). Significant content of dihydro-b-ionol (18.46%), dihydro-b-ionone (0.69%), thias­piran A (1.35%), and thiaspiran B (2.17%) were revealed in the petals of R. roulettii, which also have an influence on the aromatic bouquet of the rose oil.Conclusions. For the first time, 105 volatile organic substances were found in the petals of six species of dog-rose, 11 of which were not identified. It was found out that the largest number of components were identified in the petals of R. roulettii (49) and R. centifolia (45). The number of VOS identified in species R. multiflora (33), R. rugosa (31), and R. canina (30) was relatively smaller. Component composition of VOS in the petals of studied dog-rose species is an important constituent in breeding investigations of prospective producers for the various purposes of use.

To create an industrial hemp variety of the Central European ecological and geographical type with a high cannabigerol content and universal application. Methods. Breeding (self-pollination, creation of artificial populations, selection), field, biochemical (thin-layer and gas-liquid chromatography of cannabinoid compounds), instrumental and technological assessment of fibre quality, and statistical methods. Results. Variety ‘Vik 2020’ was obtained as a result of creation of artificial populations. The plants are characterized by higher content of cannabigerol (1.034 ± 0.0323%), and almost zero of other secondary metabolites, such as cannabidivarin, cannabidiol, cannabichromene and psychotropic tetrahydrocannabinol (0.003 ± 0.0011; 0.018 ± 0.0080; 0.012 ± 0.0027, and 0.005 ± 0.0012%, respectively). The t rait of cannabigerol content is quite stable within the population and is not correlated with the trait of tetrahydrocannabinol content (r = -0.23). TLC showed that cannabigerol accumulated mainly in the form of cannabigerolic acid and to a lesser extent as a neutral compound, which is consistent with the theory that this substance is a precursor for the synthesis of other cannabinoids. According to the results of the competitive variety test, when growing to obtain fibre and seeds, the variety features short height, specifically significantly lower total (206.4 cm) and technical stem length (135.6 cm) compared to the standard variety, significantly higher inflorescence length (70.8 cm), which determine the formation of the significant yield of biomass suitable for pharmaceutical use and high seed yield (0.98 t/ha). The yield of total fibre was the same as in the standard variety (29.0%), but its quality and technological value for primary processing were higher. The variety had a homogeneous sex structure, resistance to bioltic and abiotic environmental factors. Plants reached biological maturity in 116 days (BBCH 89). This cultivar is recommended for obtaining seeds, quality fiber and potentially cannabigerol (on condition of changes in legislation). Conclusions. The efficiency of using self-pollinating lines in breeding with their subsequent combining into a synthetic population and improving selection was proved by the case of a new variety of industrial hemp ‘Vik 2020’, characterized by an increased content of cannabigerol and the absence of psychotropic properties

Purpose. Identifying the features of the seasonal growth rhythm, development of the plants, morphological and biological differences in monoecious introduced and domestic varieties of Cannabis sativa L. in the North-east region of Ukraine. Methods. The research was conducted from 2014 till 2019. The following research methods were employed: field (phenological observations, sampling for laboratory analysis), microscopic (study of the morphological structure of glandular and cystolithic hairs on vegetative and generative organs, determination of the size and density of hair formation), laboratory and analytical (determination of the content of cannabinoid substances by thin layer and gas-liquid chromatography), biomorphological me­thods (analysis of morphological characteristics of plants). Chemical analysis for the content of cannabinoid substan­ces was carried out in the laboratory of the breeding and seed production department of the Institute of Bast Crops of the NAAS of Ukraine (Glukhiv, 2014–2019). The content of neutral substances [cannabinol (CBN), cannabidiol (CBD), tetrahydrocannabinol (THC)] and acids (CBDA, THCA) was determined by using a thin layer chromatography (TLC) and gas-liquid chromatography (GLC) methods. For the study, 11 monoecious varieties of C. sativa: ‘YuSO 1’, ‘YuSO 31’, ‘Hliana’, ‘Viktoriia’, ‘Hlesiia’, ‘Hlukhivskyi 46’, ‘Hlukhivski 51’, ‘Zoloto­niski 15’ and 3 introduced varieties: ‘Fedora 17’, ‘Felina 32’, ‘Futura 77’ (France) which differed among themselves in morphological, biological, genetic, ecological-geographical and economically valuable characteristics were selected. Results. Biological and morphological characteristics of modern monoecious varieties of C. sativa were investigated depending on the ecological and geographical zones of origin, conditions of the growing season and the stage of ontogenesis. The functional interdependence between the formation of specialized excretory structures and the content of cannabinoid substances in plants of different varieties of C. sa­tiva was revealed. The following promising varieties of C. sativa in a ration of ((THC + CBN) ≤ CBD) point the possibility to use them as the source material for creating the medicinal varie­ties of plants with a high content of CBD. Conclusions. Nowadays C. sativa is the main source of phyto material for industrial production of natural CBD. In recent years, high-yielding varieties of C. sativa have been created, which, together with high commercially valuable traits, did not contain psychoactive THC and had an insignificant amount of CBD. The results of the research indicated the features of the seasonal growth rhythm, development of the plants and the morphological and biological features of modern monoecious introduced and domestic varieties of C. sativa in the North-east region of Ukraine. The low correlation between the content of cannabinoid substances and their acids in varieties, which are cha­racterized by the low content of cannabinoid compounds was established. According to the developed own classification (THC + KBN): CBD, varieties with a predominant CBD content ‘Hlukhivskyi 46’, ‘Zolotoniski 15’, ‘Hliana’ which are of significant medicinal value were identified.

Purpose. To analyse the qualitative and quantitative composition of volatile organic substances (VOS) in the petals of six species of dog-rose in the collection of M. M. Gryshko National Botanic Garden of the NAS of Ukraine for further bree­ding. Methods. Volatile organic substances were obtained by steam distillation. VOS was determined using gas chromato­graphy (Chromatograph Agilent Technologies 6890). Results. The qualitative and quantitative composition of volatile organic substances in the petals in such species as Rosa L. (R. roullettii HCh (Correvon), R. multiflora Thunb., R. pimpinellifolia L., R. canina L., R. centifolia L., R. rugosa Thunb.) was investigated. According to the results of the research, 105 VOS were discovered in the dog-rose petals, among which 11 were not identified. The dog-rose petals of identified VOC contained 16 components which share exceeds 5%, 31 – was ranging from 1% to 5%, 47 – was less than 1%. They belonged to different groups including monoterpenoids, sesquiterpenoids, sesquiterpene alcohols, saturated unbranched hydrocarbons etc. The largest number of components were identified in the petals of R. roulettii (49) and R. centifolia (45), while the number of identified components in species R. multiflora (33), R. rugosa (31) and R. canina (30) was relatively smaller. All studied dog-rose species were characterized by the presence of such saturated unbranched hydrocarbons as decan, tetradecane, pentadecane, hexadecane, heptadecane, octadecane, nanodecane, heneicosane, tricosane, tetracosane, pentacosane, heptocosane, hentriacontane. Triterpene hydrocarbon squalene is the important component of the VOS complex in dog-rose pen tals. b-phenylethyl alcohol to be one of the main components of the rose essential oils was found during investigation in the following four dog-rose species as R. centifolia (0.61%), R. pimpinellifolia (3.56%), R. rugosa (4.24%), and R. multiflora (5.43%). Significant content of dihydro-b-ionol (18.46%), dihydro-b-ionone (0.69%), thias­piran A (1.35%), and thiaspiran B (2.17%) were revealed in the petals of R. roulettii, which also have an influence on the aromatic bouquet of the rose oil. Conclusions. For the first time, 105 volatile organic substances were found in the petals of six species of dog-rose, 11 of which were not identified. It was found out that the largest number of components were identified in the petals of R. roulettii (49) and R. centifolia (45). The number of VOS identified in species R. multiflora (33), R. rugosa (31), and R. canina (30) was relatively smaller. Component composition of VOS in the petals of studied dog-rose species is an important constituent in breeding investigations of prospective producers for the various purposes of use.

Мета. Провести дослідження зразків соняшникової олії з різним вмістом олеїнової кислоти хроматографічним методом, показати можливість застосування відносно простих, експресних та ефективних фізичних методів для конт­ролю вмісту олеїнової кислоти у процесі селекції гібридів соняшнику і в товарному виробництві під час визначення автентичності зразків високоолеїнової олії та розпізнавання її замінників. Методи. Загальноприйняті міжнародні методи ІСО, математико-статистичні. Результати. Проведено дослідження жирнокислотного складу соняшникової олії на газовому хроматографі за спрощеною процедурою, встановлено залежність ряду фізичних методів – заломлення на рефрактометрі, в’язкості та щільності – від жирнокислотного складу соняшникової олії, зокрема вмісту олеїнової кислоти. Висновки. Метод газової хроматографії виявився найінформативнішим та ефективним для контролю вмісту олеїнової кислоти на всіх етапах як селекційного процесу, так і в умовах товарного виробництва. Серед фізичних методів на особливу увагу заслуговують ті, що ґрунтуються на визначенні показників заломлення, кінематичної в’язкості та щільності, оскільки між ними та вмістом олеїнової кислоти в соняшниковій олії встановлено високу кореляційну залежність.