Purpose. To reveal the frequency and intensity of callus formation and organogenesis, the effectiveness of microclonal reproduction of various species of the genus Linum L. (Linaceae) in vitro. Methods. For in vitro induction of callus formation and organogenesis, hypocotyl segments of species Linum usitatissimum L. convar. elongatum and convar. usitatissimum, L. tenue Desf., L. bienne Mill., L. corymbulosum Pchb., L. nervosum Waldst. & Kit., L. flavum L., L. campanulatum L., L. perenne L., L. austriacum L., L. grandiflorum Desf., L. strictum L. were cultivated on Murashige and Skoog medium supplementedwith 0.05 mg/l 1-naphthylacetic acid and 1.0 mg/l 6-benzyl aminopurine at 22–24 °C, relative humidity of 60–80%,with 16 hours photoperiod (2500 flux). For microclonal reproduction Murashige and Skoog, White, Gamborg and Eveleigh media and their modifications were used. The measurement results were interpreted by the arithmetic mean, standard error for the sample mean, the leastsignificantdifference and ranked. Results. Different species of the genus Linum to a large extend are capable of forming callus and regenerating shoots under the specified cultivation conditions. The frequency of callus formation for the studied samples on the 35th day of cultivation varied within 81.25–100%, the mass of callus from one explant – 0.21–1.64 g, the frequency of organogenesis – 12.50–100%, the number of shoots – 1.8–7.6 pcs. and the height of the shoots was 0.82–2.12 cm. The following species: L. usitatissimum convar. elongatum, L. tenue, L. bienne and L. strictum were distinguished by a high intensity of callus formation. Intensive organogenesis was pecular to L. tenue, L. bienne, L. flavum, L. austriacum and L. grandiflorum. The efficiency of somaclone obtaining was quite low in L. nervosum and L. campanulatum. In total, for the microclonal reproduction of species of the genus Linum Murashige and Skoog, Gamborg and Eveleighmedia supplementedwith 12.5 g/l glucose were optimal. At the final stages of microclonal propagation, before transferring microclones in vivo, it is advisable to use White medium, which contributes to a high frequency of rhizogenesis. Varieties of L. usitatissimum convar. elongatum and convar. usitatissimum had diffe­rent responses to in vitro culture. Conclusions. The frequency and intensity of callus formation and organogenesis, the effectiveness of microclonal reproduction depended on the genotype of a particular species; therefore it is advisable to select the composition of the nutrient medium and growth regulators for each of them. Some species of the genus Linum have not yet been studied in vitro, so the obtained results allow expanding the scope of their use in practice, in particular in breeding as a new source material with somaclonal variation, interspecific crosses, and ornamental floriculture.

Purpose. Finding ways to activate the germination of sugar beet seeds, obtaining even and synchronous sprouts when applying fertilizer compositions with nanoscale elements. Methods. Vegetation and laboratory. The seeds of sugar beet were sown in prepared utensils with soil in accordance with the requirements of the methods for vegetation experiments. Fertilizers were introduced in the form of solutions with different ratios according to six microstages. Results. At 01 microstage on the BBCH scale (130 hours after sowing), an increase in the mass of beet fruits in all variants was observed - in the control variant by 9.78%; in the application of nanofertilizers - 20,4-23,7%. The diameter of the fruit varied similarly to changes in mass: in the control variant, the diameter change was 4.95%; in variants with application of nanofertilizers — 9.56-13.9%. Changes in the rate of sprout organs formation and their linear dimensions were noted in the various fertilization schemes. The length of the embryonic root at 05 microstage with uniform introduction of high norms of zinc and phosphorus, after 40 hours after sowing, was 0.540-2.671 mm. For other fertilizer combinations, the appearance of the germ root was noted only 44 hours after sowing. In 60 hours after sowing (07 microstage on the BBCH scale) there was a complete exit of cotyledons from the socket of the cluster with the introduction of nano chelate microfertilizers and only the beginning of the exit of cotyledons in the control variant. Due to the intensive processes of swelling and germination, the growth of the primary root of the sugar beet was accelerated. Conclusions. Uniform provision of seeds with zinc and especially phosphorus on the background of basic complex fertilizer with nanoscale elements contributed to the activation of seed germination and the intense formation of synchronously developed shoots. On average, the opening of the pericarp lid and the appearance of the root accelerated for 4 hours; 6 hours earlier there was an exit of cotyledons. With the introduction of nano chelate fertilizers, root growth and elongation of the hypocotyl at the first microstages of sugar beet sprouting were accelerated twice, due to which the sugar beet sprouts appeared 4-6 hours earlier. Nano chelate microfertilizers, promoting even and synchronous germination, development of sugar beet seedlings ensured synchronous emergence of seedlings and formation of predetermined sowing density without further reduction of plants.