Purpose. To define spring barley breeding lines with an optimal combination of yielding capacity and stability under different weather conditions in the Central Forest-Steppe zone of Ukraine. Methods. Field studies, ANOVA, AMMI, GGE biplot analysis. Results. Hydrothermal regime during interphase periods of spring barley vegetation under conditions of The V. M. Remeslo Myronivka Institute of Wheat of NAAS in 2012–2014 was characterized by significant variability, that facilitated detailed evaluation of the lines for productivity, stability, and resistance to abiotic and biotic factors. In the performance of an experiment, the highest average yielding capacity (5.87 t/ha) was noted in 2012, the lowest one (3.50 t/ha) was in 2013. As for these years, average yield of 4.63 t/ha was obtained in 2014. By applying AMMI and GGE biplot analysis, significant differences in response of the studied lines to variability of weather conditions was revealed. Using AMMI model, additive components of the main effects of the breeding lines and years of testing as well as multiplicative components of their interaction were characterized. GGE biplot genotypes ranking in relation to a hypothetical “ideal” genotype showed an absolute advantage of breeding line ‘Nutans 4540’ for yielding capacity and stability. In addition, breeding lines ‘Nutans 4241’ and ‘Nutans 4120’ were close to ideatype. Selected breeding lines were characterized by resistance and moderate resistance to leaf diseases and lodging. Conclusions. Use of AMMI and GGE biplot analysis to evaluate breeding lines at the final stages of breeding process allows to describe them thoroughly and graphically as well as differentiate them not only for average yielding capacity but also for their interaction with changing conditions during the years of testing. Breeding lines ‘Nutans 4540’, ‘Nutans 4241’, and ‘Nutans 4120’ to be identified for the stability of yielding capacity display in combination with other agronomic characters were transferred to the State variety testing as new spring barley varieties ‘MIP Myrnyi’, ‘MIP Saliut’, and ‘MIP Sotnyk’ respectively.

Purpose. To define spring barley breeding lines with an optimal combination of yielding capacity and stability under different weather conditions in the Central Forest-Steppe zone of Ukraine. Methods. Field studies, ANOVA, AMMI, GGE biplot analysis. Results. Hydrothermal regime during interphase periods of spring barley vegetation under conditions of The V. M. Remeslo Myronivka Institute of Wheat of NAAS in 2012–2014 was characterized by significant variability, that facilitated detailed evaluation of the lines for productivity, stability, and resistance to abiotic and biotic factors. In the performance of an experiment, the highest average yielding capacity (5.87 t/ha) was noted in 2012, the lowest one (3.50 t/ha) was in 2013. As for these years, average yield of 4.63 t/ha was obtained in 2014. By applying AMMI and GGE biplot analysis, significant differences in response of the studied lines to variability of weather conditions was revealed. Using AMMI model, additive components of the main effects of the breeding lines and years of testing as well as multiplicative components of their interaction were characterized. GGE biplot genotypes ranking in relation to a hypothetical “ideal” genotype showed an absolute advantage of breeding line ‘Nutans 4540’ for yielding capacity and stability. In addition, breeding lines ‘Nutans 4241’ and ‘Nutans 4120’ were close to ideatype. Selected breeding lines were characterized by resistance and moderate resistance to leaf diseases and lodging. Conclusions. Use of AMMI and GGE biplot analysis to evaluate breeding lines at the final stages of breeding process allows to describe them thoroughly and graphically as well as differentiate them not only for average yielding capacity but also for their interaction with changing conditions during the years of testing. Breeding lines ‘Nutans 4540’, ‘Nutans 4241’, and ‘Nutans 4120’ to be identified for the stability of yielding capacity display in combination with other agronomic characters were transferred to the State variety testing as new spring barley varieties ‘MIP Myrnyi’, ‘MIP Saliut’, and ‘MIP Sotnyk’ respectively.

Purpose. To study the effect of AKM plant growth regulator on growth, development and yield formation of sunflower (Helianthus annuus L.) hybrids in hybridization plots under the conditions of the Southern Steppe zone of Ukraine. Methods. Laboratory tests, field study, statistical evaluation. Results. The results of studies devoted to determining the optimal AKM concentration for the treatment of seeds of the maternal and paternal lines, the effect of AKM on field germination, biometric parameters of sunflower plants, seed quality (F1) and yield are presented. Three hybrids of the Ukrainian selection, such as ‘Alpha’, ‘Logos’ and ‘Persei’ were studied during 2014–2016. Optimal concentration of AKM (0.0015 g/l) was defined. The vigor of seeds processed by AKM was higher than in check variety by 0.8–12.8 р.р. (♂); 0.4–10.7 p.p. (♀), laboratory germination – by 2.3–6.1 p.p. (♂); 3.5–6.2 p.p. (♀). In 2016, the sunflower plant height for all variants exceeded this parameter to be obtained for other years of the study. This could be explained by the fact that HTC in 2016 for the BBCH 00–39 period was 1.4 times higher than in 2015. In general, hybrids as the studied factor considerably influenced sunflower yield, and the share of the hybrid (factor A) influence is 33%. This should be considered when selecting hybrids for sunflower cultivation technologies in the Steppe zone of Ukraine. Conclusions. Hydrothermal conditions of the year had the maximum impact on the formation of seed quality and yield of sunflower plants of the hybrids under study, but when using AKM growth regulator for presowing seed treatment, this negative impact was reduced by an average of 23%.

Цель. Установить особенности накопления каннабидиола и других каннабиноидных соединений в онтогенезе технической (промышленной) однодомной конопли, определить оптимальную фенологических фазу конопли, в которую целесообразно проводить уборку растений с целью получения каннабидиола для нужд фармацевтической отрасли. Методы. Полевые, лабораторные (тонкослойная хроматография), математической статистики (корреляционно-регрессионный анализ). Результаты. Приведены результаты трехлетних исследований накопления биомассы в онтогенезе растений конопли сортов ‘Гляна’, ‘ЮСО 31’ и ‘Золотоніські 15’, проявления содержания каннабидиола, тетрагидроканнабинола и канабинола в средневзвешенном образце вегетативной массы всех живых листьев и соцветия. Выводы. Наличие и интенсивность накопления определенного каннабиноидного соединения являются наследственно обусловленными признаками. Небольшое количество каннабиноидов у конопли можно идентифицировать уже на ранних стадиях развития, в частности в фазе 1–3 пар настоящих листьев. На основе теоретических расчетов и согласно проведенных исследований установлено, что оптимальным периодом для сбора биомассы конопли медицинского направления использования с последующим выделением из нее каннабидиола является период от полного цветения до фазы биологической спелости. В отдельных семей сорта ‘Гляна’, с которыми проводилась селекционная работа в направлении повышения содержания каннабидиола, можно получить около 5,808 г/м2, сорта ‘ЮСО 31’ – 1,528 г/м2, сорта ‘Золотоніські 15’ – 1,563 г/м2 данного вещества. Соцветия конопли содержат гораздо больше каннабиноидных соединений по сравнению с листьями, но ввиду их доли в общей биомассе растений можно утверждать, что и соцветия, и листья в равной степени пригодны для использования в качестве источника каннабидиола. Использование технической (промышленной) конопли в медицинской (фармацевтической) отраслях является перспективным, но при условии целенаправленной селекционной работы. | Purpose. To find out the specifics of cannabidiol and other cannabinoid compounds accumulation during the ontogenesis of industrial monoecious hemp (i); to determine the optimal phenological stage of harvesting for the purpose of obtaining cannabidiol for pharmaceutical purposes (ii); to find out the prospects of using hemp for the needs of pharmaceutical industry (iii).Methods. Field, labo­ratory (thin-layer chromatography), statistics (correlation and regression analysis).Results. The results of three-year research on the dynamics of biomass accumulation in the ontogenesis of hemp plants of the ‘Hliana’, ‘USO 31’ and ‘Zolotoniski 15’ varieties are presented. The manifestation of the content of cannabidiol, tetrahydrocannabinol and cannabinol is given not in terms of their maximum content (using the analysis of apical parts of plants or female flo­wers) but using a weighted average sample of the vegetative mass from all live leaves and inflorescences.Conclusions. The presence and intensity of certain cannabinoid compound accumulation are the hereditary signs. A small number of cannabinoids in hemp can be identified already at early stages of development, in particular at the 1–3 true leaf pairs stage. On the basis of theoretical calculations and in accordance with the described research methodology, it was found that the optimal period for harvesting biomass of pharmaceutical hemp with subsequent release of cannabidiol is the period from full flowering to the stage of biological maturity. Certain families of the ‘Hliana’ variety involved into selection for increasing cannabidiol content can produce about 5.808 g/m2 of the active substance. The families of variety ‘USO 31’ can produce 1.528 g/m2 and the ‘Zolotoniski 15’ 1.563 g/m2 of the active substance. Inflorescences of hemp contain much more cannabinoid compounds compared to leaves; however, taken into account their shares in the total biomass of plants, it can be argued that inflorescences and leaves are equally suitable for use as a source of cannabidiol. The use of industrial hemp in the pharmaceutical industry is promising but on the assumption of the target breeding | Мета. Встановити особливості накопичення канабідіолу та інших канабіноїдних сполук під час онтогенезу технічних (промислових) однодомних конопель, визначити оптимальну фенологічну фазу конопель, у яку доцільно проводити збирання рослин з метою отримання канабідіолу для потреб фармацевтичної галузі.Методи. Польові, лабораторні (тонкошарова хроматографія), математичної статистики (кореляційно-регресійний аналіз).Результати. Наведено результати трирічних досліджень із вивчення накопичення біомаси в онтогенезі рослин конопель сортів ‘Гляна’, ‘ЮСО 31’ і ‘Золотоніські 15’, вмісту канабідіолу, тетрагідроканабінолу і канабінолу в середньозваженому зразку вегетативної маси усіх живих листків і суцвіття.Висновки. Наявність та інтенсивність накопичення певної канабіноїдної сполуки є спадково обумовленими ознаками. Невелику кількість канабіноїдів у конопель можна ідентифікувати вже на ранніх стадіях розвитку, зокрема у фазі 1–3 пар справжніх листків. На основі теоретичних розрахунків і згідно проведених досліджень установлено, що оптимальним періодом для збирання біомаси конопель медичного напряму використання та подальшого виділення канабідіолу є період від повного цвітіння до фази біологічної стиглості. У окремих сімей сор­ту ‘Гляна’, з якими проводилась селекційна робота у напрямі підвищення вмісту канабідіолу, можна отримати близько 5,808 г/м2, сорту ‘ЮСО 31’ – 1,528 г/м2, сорту ‘Золотоніські 15’ – 1,563 г/м2 даної речовини. Суцвіття конопель містять набагато більше канабіноїдних сполук, порівняно з листками, але зважаючи на їх частку в загальній біомасі рослин, можна стверджувати, що і суцвіття, і листки однаковою мірою придатні для використання як джерела канабідіолу. Використання технічних (промислових) конопель у медичній (фармацевтичній) галузях є перспективним, але за умови цілеспрямованої селекційної роботи.

Purpose. To analyze the level of cross-resistance of obtained salt- and osmotolerant cell lines and plants regenerants of winter triticale to osmotic and salt stresses. Methods. Cultures of tissue and organs in vitro, in vitro breeding, biochemical, statistical analysis. Results. It was established that the stability of cross-resistance trait display to saline and osmotic stresses in obtained cell lines of winter triticale was rather high – from 50 to 76% of calli have survived to the end of the sixth passage. It has been shown that despite the presence of sublethal concentrations of the stress-factor (mannitol/sodium chloride) in selective medium, stable cell lines of the triticale actively continued to grow and accumulate biomass. It was found that in the line ‘38/1296’ cell lines 5L/sl and 5L/os respectively were the most resistant to osmotic and salt stresses, and lines 1C/s1 and 1C/os respectively in the ‘Obrii’ variety, since they had the highest percent of living calli and biomass increment under the selective conditions and their plant regenerant – the highest level of survival after the impact of the abiotic stressors complex. The salt-resistant cell lines of both genotypes of winter triticale as compared to the control were also characterized by significantly higher free proline content under the selective factors impact. The results obtained may indicate that the cell lines and triticale plant regenerants have a genetically determined trait of resistance to stress factors. Conclusions. Verification of traits of resistance to abiotic stressors has shown a significantly high level of cross-tolerance of the obtained cell lines of both triticale genotypes for saline and osmotic stresses. Resistance to saline and osmotic stresses of cells separated in vitro was preserved in induced plants and at the organism level has increased tolerance to abiotic environmental factors. It is shown that due to the general non-specific mechanisms of resistance, the capacity of the callus cultures of triticale to resist to one abiotic stressor can lead to increased tolerance for another one.

Мета. Проаналізувати рівень перехресної стійкості отриманих соле- та осмостійких клітинних ліній і рослин-регенерантів тритикале озимого до осмотичного та сольового стресів.Методи. Культури тканин і органів in vitro, селекції in vitro, біохімічні, статистичний аналіз.Результати. Встановлено, що стабільність прояву ознаки перехресної стійкості як до сольового, так і до осмотичного стресів у отриманих клітинних ліній тритикале озимого була на досить високому рівні – до кінця шостого пасажу виживало від 50 до 76% калюсів. Показано, що, незважаючи на наявність у селективному середовищі сублетальної концентрації стрес-фактора (маніту/хлориду натрію), стійкі клітинні лінії тритикале активно продовжували рости й накопичувати біомасу. Виявлено, що у лінії ‘38/1296’ найбільш стійкими до осмотичного та сольового стресів були клітинні лінії 5Л/сл та 5Л/ос відповідно, а в сорту ‘Обрій’ – 1С/сл та 1С/ос відповідно, оскільки вони мали найвищу частку живих калюсів та приріст біомаси за селективних умов, а рослини-регенеранти з них – найвищий рівень виживання після дії комплексу абіотичних стресорів. Солестійкі клітинні лінії обох генотипів тритикале озимого порівняно з контролем характеризувались також достовірно вищим вмістом вільного проліну за дії селективних чинників. Одержані результати можуть свідчити про те, що клітинні лінії та рослини-регенеранти тритикале мають генетично обумовлену ознаку стійкості до стресових факторів.Висновки. Перевірка ознак стійкості до абіотичних стресорів засвідчила досить високий рівень перехресної толерантності отриманих клітинних ліній обох генотипів тритикале як до сольового, так і до осмотичного стресів. Стійкість до сольового та осмотичного стресів виділених in vitro клітин збереглась у індукованих рослинах і на організмовому рівні забезпечила підвищення толерантності до абіотичних факторів середовища. Показано, що завдяки загальним неспецифічним механізмам стійкості резистентність калюсних культур тритикале до одного абіотичного стресора може призводити до підвищення толерантності й до іншого. | Purpose. To analyze the level of cross-resistance of obtained salt- and osmotolerant cell lines and plants regenerants of winter triticale to osmotic and salt stresses. Methods. Cultures of tissue and organs in vitro, in vitro breeding, biochemical, statistical analysis. Results. It was established that the stability of cross-resistance trait display to saline and osmotic stresses in obtained cell lines of winter triticale was rather high – from 50 to 76% of calli have survived to the end of the sixth passage. It has been shown that despite the presence of sublethal concentrations of the stress-factor (mannitol/sodium chloride) in selective medium, stable cell lines of the triticale actively continued to grow and accumulate biomass. It was found that in the line ‘38/1296’ cell lines 5L/sl and 5L/os respectively were the most resistant to osmotic and salt stresses, and lines 1C/s1 and 1C/os respectively in the ‘Obrii’ variety, since they had the highest percent of living calli and biomass increment under the selective conditions and their plant regenerant – the highest level of survival after the impact of the abiotic stressors complex. The salt-resistant cell lines of both genotypes of winter triticale as compared to the control were also characterized by significantly higher free proline content under the selective factors impact. The results obtained may indicate that the cell lines and triticale plant regenerants have a genetically determined trait of resistance to stress factors.Conclusions. Verification of traits of resistance to abiotic stressors has shown a significantly high level of cross-tolerance of the obtained cell lines of both triticale genotypes for saline and osmotic stresses. Resistance to saline and osmotic stresses of cells separated in vitro was preserved in induced plants and at the organism level has increased tolerance to abiotic environmental factors. It is shown that due to the general non-specific mechanisms of resistance, the capacity of the callus cultures of triticale to resist to one abiotic stressor can lead to increased tolerance for another one. | Цель. Проанализировать уровень перекрестной устойчивости полученных соле- и осмоустойчивых клеточных линий и растений-регенерантов тритикале озимого к осмотическому и солевому стрессам. Методы. Культуры тканей и органов in vitro, селекции in vitro, биохимические, статистический анализ. Результаты. Установлено, что стабильность проявления признака перекрестной устойчивости как к солевому, так и осмотическому стрессам у полученных клеточных линий тритикале озимого была на достаточно высоком уровне – к концу шестого пассажа выживало от 50 до 76% каллусов. Показано, что, несмотря на наличие в селективной среде сублетальной концентрации стресс-фактора (маннита/хлорида натрия), устойчивые клеточные линии тритикале активно продолжали свой рост и накапливали биомассу. Выявлено, что в линии ‘38/1296’ наиболее устойчивыми к осмотическому и солевому стрессам были клеточные линии 5Л/сл и 5Л/ос соответственно, а у сорта ‘Обрий’ – 1С/сл и 1С/ос соответственно, поскольку они имели самую высокую долю живых каллусов и прирост биомассы в селективных условиях, а растения-регенеранты из них – наивысший уровень выживания после воздействия комплекса абиотических стрессоров. Солеустойчивые клеточные линии обоих генотипов тритикале озимого по сравнению с контролем характеризовались также достоверно более высоким содержанием свободного пролина при действии селективных факторов. Полученные результаты могут свидетельствовать о том, что клеточные линии и растения-регенеранты тритикале имеют генетически обусловленный признак устойчивости к стрессовым факторам. Выводы. Проверка признаков устойчивости к абиотическим стрессорам показала достаточно высокий уровень перекрестной толерантности полученных клеточных линий обоих генотипов тритикале как к солевому, так и к осмотическому стрессам. Устойчивость к солевому и осмотическому стрессам выделенных in vitro клеток сохранилась в индуцированных растениях и на уровне организма обеспечила повышение толерантности к абиотическим факторам среды. Показано, что благодаря общим неспецифическим механизмам устойчивости резистентность каллусных культур тритикале к одному абиотическому стрессору может приводить к повышению толерантности и к другому.

Purpose. To analyze the level of cross-resistance of obtained salt- and osmotolerant cell lines and plants regenerants of winter triticale to osmotic and salt stresses. Methods. Cultures of tissue and organs in vitro, in vitro breeding, biochemical, statistical analysis. Results. It was established that the stability of cross-resistance trait display to saline and osmotic stresses in obtained cell lines of winter triticale was rather high – from 50 to 76% of calli have survived to the end of the sixth passage. It has been shown that despite the presence of sublethal concentrations of the stress-factor (mannitol/sodium chloride) in selective medium, stable cell lines of the triticale actively continued to grow and accumulate biomass. It was found that in the line ‘38/1296’ cell lines 5L/sl and 5L/os respectively were the most resistant to osmotic and salt stresses, and lines 1C/s1 and 1C/os respectively in the ‘Obrii’ variety, since they had the highest percent of living calli and biomass increment under the selective conditions and their plant regenerant – the highest level of survival after the impact of the abiotic stressors complex. The salt-resistant cell lines of both genotypes of winter triticale as compared to the control were also characterized by significantly higher free proline content under the selective factors impact. The results obtained may indicate that the cell lines and triticale plant regenerants have a genetically determined trait of resistance to stress factors. Conclusions. Verification of traits of resistance to abiotic stressors has shown a significantly high level of cross-tolerance of the obtained cell lines of both triticale genotypes for saline and osmotic stresses. Resistance to saline and osmotic stresses of cells separated in vitro was preserved in induced plants and at the organism level has increased tolerance to abiotic environmental factors. It is shown that due to the general non-specific mechanisms of resistance, the capacity of the callus cultures of triticale to resist to one abiotic stressor can lead to increased tolerance for another one.

Purpose. To conduct in vitro screening of different genotypes of winter triticale for resistance to salinity in the shoot apical meristem culture. Methods. Plant tissue culture in vitro, in vitro breeding, statistical analysis. Results. It was found that the increase of sodium chloride concentration from 0.6 to 1.5% resulted in inhibition of the callus culture growth in all genotypes that was indicative of the toxic effect of the stress factor. It turns out that 1.2% sodium chloride concentration allowed to differentiate triticale genotypes for salt tolerance. The line ‘38/1296’ appeared to be the most resistant to salinity stress because under breeding conditions calli of this genotype were characterized by higher morphogenetic potential, had the highest crude mass increase, and plants-regenerants were obtained only from explants of this line after cultivation on the medium containing 1.5% sodium chloride. The ‘ADM 11’ variety was the most sensitive to saline stress as mass necrosis and lack of regenerative ability in its calli were observed under breeding conditions. In the studied forms, genotypic dependence of morphogenesis processes in vitro culture was registered. From the induced calli, plants-regenerants were obtained, and their completion of growing, root development and transfer to in vivo conditions were optimized. Conclusions. Genotypic response to salinity stress in the culture of shoot apical meristems of winter triticale was expressed by various crude mass increase and different morphogenetic potential on exposure to a stress factor. The line ‘38/1296’ can be used as a valuable material for further breeding of winter triticale. The culture of shoot apical meristems is recommended to apply as a test system for screening of triticale genotypes for resistance to salinity stress

Purpose. To conduct in vitro screening of different genotypes of winter triticale for resistance to salinity in the shoot apical meristem culture. Methods. Plant tissue culture in vitro, in vitro breeding, statistical analysis. Results. It was found that the increase of sodium chloride concentration from 0.6 to 1.5% resulted in inhibition of the callus culture growth in all genotypes that was indicative of the toxic effect of the stress factor. It turns out that 1.2% sodium chloride concentration allowed to differentiate triticale genotypes for salt tolerance. The line ‘38/1296’ appeared to be the most resistant to salinity stress because under breeding conditions calli of this genotype were characterized by higher morphogenetic potential, had the highest crude mass increase, and plants-regenerants were obtained only from explants of this line after cultivation on the medium containing 1.5% sodium chloride. The ‘ADM 11’ variety was the most sensitive to saline stress as mass necrosis and lack of regenerative ability in its calli were observed under breeding conditions. In the studied forms, genotypic dependence of morphogenesis processes in vitro culture was registered. From the induced calli, plants-regenerants were obtained, and their completion of growing, root development and transfer to in vivo conditions were optimized. Conclusions. Genotypic response to salinity stress in the culture of shoot apical meristems of winter triticale was expressed by various crude mass increase and different morphogenetic potential on exposure to a stress factor. The line ‘38/1296’ can be used as a valuable material for further breeding of winter triticale. The culture of shoot apical meristems is recommended to apply as a test system for screening of triticale genotypes for resistance to salinity stress

Purpose. To summarize results of studying consumptive qualities of different varieties of potato and define basic characteristics which allow to subsume them under specific economic categories. Methods. Field study, laboratory test, summarizing, analytical approach. Results. Potato varieties entered in the State register of plant varieties suitable to dissemination in Ukraine and new ones especially to be bred at the Institute of Potato Growing of NAAS were studied during the period of 2005–2016 for such basic economic characters as consumptive quality of tubers, content of starch, dry matters, protein, sugar, vitamins, carotenoids and mineral substances as well as aminoacids, color of flesh, suitability for industrial manufacturing of potato products and for purpose of technology. Attention was paid to the good prospects to use varie­ties with purple, blue and red potato tuber flesh with high antioxidant capacity. Potato varieties with above cha­racteristics and their complex combination were defined and described. The requirements of processing industry for potato as a raw material for manufacturing of potato food were given. Conclusions. The major criterion for consumptive qualities of a potato variety and correspondingly division for the commercial use is consumptive quality of tubers, especially content of essential nutrients and their favorable combination, improved taste and cooking quality, high antioxidant capacity, suitability for potato products manufacturing and use for purpose of technology. Potato varieties can be divided for economic purposes into edible, suitable for potato products manufacturing, technical and multipurpose ones.

Purpose. To summarize results of studying consumptive qualities of different varieties of potato and define basic characteristics which allow to subsume them under specific economic categories. Methods. Field study, laboratory test, summarizing, analytical approach. Results. Potato varieties entered in the State register of plant varieties suitable to dissemination in Ukraine and new ones especially to be bred at the Institute of Potato Growing of NAAS were studied during the period of 2005–2016 for such basic economic characters as consumptive quality of tubers, content of starch, dry matters, protein, sugar, vitamins, carotenoids and mineral substances as well as aminoacids, color of flesh, suitability for industrial manufacturing of potato products and for purpose of technology. Attention was paid to the good prospects to use varie­ties with purple, blue and red potato tuber flesh with high antioxidant capacity. Potato varieties with above cha­racteristics and their complex combination were defined and described. The requirements of processing industry for potato as a raw material for manufacturing of potato food were given. Conclusions. The major criterion for consumptive qualities of a potato variety and correspondingly division for the commercial use is consumptive quality of tubers, especially content of essential nutrients and their favorable combination, improved taste and cooking quality, high antioxidant capacity, suitability for potato products manufacturing and use for purpose of technology. Potato varieties can be divided for economic purposes into edible, suitable for potato products manufacturing, technical and multipurpose ones.

Purpose. To determine morphological and morphometric features of vegetative and generative organs of Persica species and varieties under the conditions of the Forest-Steppe zone of Ukraine for further use in breeding. Methods. Biological (morphological analysis) and statistical (processing of morphometric parameters) ones. Results. It was found that except ternate multiple buds, in peach hybrid ‘Suputnyk’collateral buds were prevalent which was not common to other tested varieties and species. Buds of each species and variety have a certain number of scales. The most scales were observed in ‘Suputnyk’. Pubescence of abaxial side of the leaf in P. davіdiana and ‘Suputnyk’ plants was not revealed. Species of P. vulgaris (‘Pecherskyi’, ‘Antotsianovyi’, ‘Poleskyi’) was characterized by increase in size of leaf blades, flowers and fruits. The distinctive features of P. davidiana and ‘Suputnyk’ are that stamens were accumulated in their flowers while in P. vulgaris they are branched. Raphe of a P. vulgaris stone protrudes above the edges of the bordering creases and consists of a number of narrow plates. Conclusions. For the breeding purposes, plants have an advantage for some basic criteria: the most flower buds that is common to interspecies hybrid ‘Suputnyk’; increase in photosynthetic productivity of the leaf apparatus to be typical for the variety ‘Pecherskyi’; large fruits that ‘Pecherskyi’ variety is noted for; intense red color of fruits that is inherent feature of ‘Antocianovyi’ variety.

The symposium “One fungus = Which name” held in Amsterdam 12–13 April 2012, addressed the drastic changes in the naming of pleomorphic fungi adopted by the 18th International Botanical Congress in Melbourne in 2011. Possible solutions and ways to face resulting problems were suggested. The fundamental change is that under the new rules fungi in future will be treated nomenclaturally like plants and all other groups of organisms ruled by the ICN, i.e. with one correct name for each species. Numerous discussions and statements during the Symposium reflected widespread anxieties that these rules could negatively influence taxonomic work on pleomorphic fungi. However, they are groundless, being based on misunderstandings and confusion of nomenclature and taxonomy. With pleomorphic fungi, taxonomists will in future have to answer the question whether different morphs can represent one fungus (taxon), but this remains a taxonomic decision and has nothing to do with nomenclature. Furthermore, the ICN does not and cannot rule on how this decision is made. Thus it cannot provide rules based solely on methods involving morphology in vivo or in vitro, molecular analyses, physiological and biochemical data, inoculation experiments in pathogenic groups or any other methods or combinations of them. It is up to the taxonomist to select appropriate methods and to decide which data are sufficient to introduce new taxa. Some future problems and strategies around the application of anamorph- and teleomoph-typified taxon names (genera and species), arediscussed here, using the recently monographed powdery mildews (Erysiphales) as an example.

Purpose. To review publications relating to the key point of the genotyping technology that is competitive allele-specific polymerase chain reaction (which is called now Kompetitive Allele Specific PCR, KASPTM) and its use in various genetic-breeding researching (a study of maize). Results. The essence of KASP-genotyping, its advantages are highlighted. The requirements for matrix DNA are presented, since the success of the KASP-analysis depends on its qua­lity and quantity. Examples of global projects of plant breeding for increasing crop yields using the KASP genoty­ping technology are given. The results of KASP genotyping and their introduction into breeding and seed production, in particular, for determining genetic identity, genetic purity, origin check, marker-assisted selection, etc. are presented using maize as an example. It is demonstrated how geno­mic selection according to KASP genotyping technology can lead to rapid genetic enhancement of drought resistance in maize. Comparison of the effectiveness of creating lines with certain traits (for example, combination of high grain yield and drought resistance) using traditional breeding approaches (phenotype selection) and molecular genetic methods (selection by markers) was proved that it takes four seasons (two years in case of greenhouses) in order to unlock the potential of the plant genotype using traditional self-pollination, test-crossing and definitions), while using markers, the population was enriched with target alleles during one season. At the same time, there was no need for a stress factor. Conclusions. KASP genotyping technology is a high-precision and effective tool for modern genetics and breeding, which is successfully used to study genetic diversity, genetic relationship, population structure, gene­tic identity, genetic purity, origin check, quantitative locus mapping, allele mapping, marker-assisted selection, marker-assisted breeding. It is expedient and timely to introduce KASP genotyping technology in our country to solve a wide range of modern genetics, breeding, seed production tasks.

Purpose. To review publications relating to the key point of the genotyping technology that is competitive allele-specific polymerase chain reaction (which is called now Kompetitive Allele Specific PCR, KASPTM) and its use in various genetic-breeding researching (a study of maize). Results. The essence of KASP-genotyping, its advantages are highlighted. The requirements for matrix DNA are presented, since the success of the KASP-analysis depends on its qua­lity and quantity. Examples of global projects of plant breeding for increasing crop yields using the KASP genoty­ping technology are given. The results of KASP genotyping and their introduction into breeding and seed production, in particular, for determining genetic identity, genetic purity, origin check, marker-assisted selection, etc. are presented using maize as an example. It is demonstrated how geno­mic selection according to KASP genotyping technology can lead to rapid genetic enhancement of drought resistance in maize. Comparison of the effectiveness of creating lines with certain traits (for example, combination of high grain yield and drought resistance) using traditional breeding approaches (phenotype selection) and molecular genetic methods (selection by markers) was proved that it takes four seasons (two years in case of greenhouses) in order to unlock the potential of the plant genotype using traditional self-pollination, test-crossing and definitions), while using markers, the population was enriched with target alleles during one season. At the same time, there was no need for a stress factor. Conclusions. KASP genotyping technology is a high-precision and effective tool for modern genetics and breeding, which is successfully used to study genetic diversity, genetic relationship, population structure, gene­tic identity, genetic purity, origin check, quantitative locus mapping, allele mapping, marker-assisted selection, marker-assisted breeding. It is expedient and timely to introduce KASP genotyping technology in our country to solve a wide range of modern genetics, breeding, seed production tasks.

Purpose. To define morphological peculiarities of the different types of shoots of switch grass (Panicum virgatum L.) in the context of introduction in the Right-Bank Forest-Steppe and Polissia zones of Ukraine. Methods. Morpho-physiological and ontogenetic analysis, morphometric technique, statistical evaluation. Results. It was found that P. virgatum plants were producing the following types of shoots: vegetative-generative short or long rhizomatous ones (they appeared as in the underground part of a maternal shoot in «the area of shortened internodes» and in the base of its aboveground part); vegetative underground and aboveground ones (they are forming both in the axils of scales of the underground part of a maternal shoot and in the axils of cauline leaves); generative aboveground shoots (borne in the axil of cauline leaves). Morphological parameters and features of morphogenesis of reproduction, resting and secondary buds have been described. Conclusions. During flowering phase of maternal shoot, P. virgatum reproduction buds were on the organogenesis phase I–II, their bursting occured after maternal shoot has flowered. The correlation of internodes length, leave blades and sheaths has been established. The maximum values of the above parameters were observed in the middle part of the prefloral zone of the stem. In the context of introduction, panicle earing came in the third decade of July, flowering began in the first decade of August. It was revealed that complete flowers are bloomed faster than the staminate ones.

Purpose. To define morphological peculiarities of the different types of shoots of switch grass (Panicum virgatum L.) in the context of introduction in the Right-Bank Forest-Steppe and Polissia zones of Ukraine. Methods. Morpho-physiological and ontogenetic analysis, morphometric technique, statistical evaluation. Results. It was found that P. virgatum plants were producing the following types of shoots: vegetative-generative short or long rhizomatous ones (they appeared as in the underground part of a maternal shoot in «the area of shortened internodes» and in the base of its aboveground part); vegetative underground and aboveground ones (they are forming both in the axils of scales of the underground part of a maternal shoot and in the axils of cauline leaves); generative aboveground shoots (borne in the axil of cauline leaves). Morphological parameters and features of morphogenesis of reproduction, resting and secondary buds have been described. Conclusions. During flowering phase of maternal shoot, P. virgatum reproduction buds were on the organogenesis phase I–II, their bursting occured after maternal shoot has flowered. The correlation of internodes length, leave blades and sheaths has been established. The maximum values of the above parameters were observed in the middle part of the prefloral zone of the stem. In the context of introduction, panicle earing came in the third decade of July, flowering began in the first decade of August. It was revealed that complete flowers are bloomed faster than the staminate ones.

Мета. Виявити закономірності рівня прояву врожайності сортів ячменю ярого у взаємодії «генотип–середовище» за випробування в різних екологічних зонах України та виділити генотипи з підвищеним адаптивним потенціалом. Методи. Об’єкт досліджень – 36 сортів ячменю ярого вітчизняної та зарубіжної селекції. Сортовипробування проведено в Миронівському інституті пшениці імені В. М. Ремесла НААН України (МІП) (Центральний Лісостеп) у 2015–2017 рр., на Носівській селекційно-дослідній станції МІП (НСДС) (Полісся) та на Кіровоградській державній сільськогосподарській дослідній станції НААН (КДСГДС) (Північний Степ) у 2016–2017 рр. За три роки досліджень (2015–2017) отримано результати випробування сортів у семи середовищах. Ділянки обліковою площею 10 м2 закладали у триразовій повторності методом повних рендомізованих блоків, відповідно до загальноприйнятих методик. Статистичний аналіз експериментальних даних проведено з використанням комп’ютерних програм Excel 2010 і Statistica 8.0. Для наочної інтерпретації взаємодії «генотип–середовище» використано GGE biplot модель. Результати. Дисперсійний аналіз даних урожайності засвідчив достовірні внески у загальну варіацію середовища – 64,64%, гено­типу – 14,90% та їх взаємодії – 20,46%. Найвищою диференціювальною здатністю (інформативністю) характеризувались умови МІП у 2016 р., найнижчою – умови КДСГДС у 2017 р. Найбільш репрезентативними були умови МІП у 2017 р. і НСДС у 2016 р., найменш репрезентативними – у КДСГДС (2016 р.). Найвіддаленішими між собою були умови МІП та KДСГДС у 2016 р. Візуалізація GGE biplot «хто-де-переміг» дала змогу розподілити середовища на два сектори: перший – умови МІП 2015–2017 рр. і НСДС 2016–2017 рр., другий – умови КДСГДС 2016–2017 рр. У першому секторі суттєву перевагу мав сорт ‘МІП Мирний’, у другому – сорт ‘Скарб’. Диференційовано й виділено сорти ячменю ярого з оптимальним рівнем прояву врожайності в середовищах, найближчих до гіпотетичного «ідеального» генотипу GGE biplot моделі – ‘МІП Мирний’, ‘МІП Богун’, ‘Талісман Миронівський’, ‘МІП Азарт’, ‘Доказ’, ‘Пан’. Висновки. Моделювання багатосередовищних сортовипробувань шляхом комбінування контрастних за гідротермічним режимом років і різних екологічних умов з інтерпретацією результатів досліджень за сучасними статистично-графічними методами сприяє детальнішій характеристиці взаємодії «генотип–середовище», ранжируванню і виділенню перспективних генотипів.

Цель. Выявить закономерности уровня проявления урожайности сортов ячменя ярового во взаимодействии «генотип–среда» при испытании в разных экологичес­ких зонах Украины и выделить генотипы с повышенным адаптивным потенциалом.Методы. Объект исследований – 36 сортов ячменя ярового отечественной и зарубежной селекции. Сортоиспытания проведены в Мироновском институте пшеницы имени В. Н. Ремесло НААН Украины (МИП) (Центральная Лесостепь) в 2015–2017 гг., на Носовской селекционно-опытной станции МИП (НСОС) (Полесье) и Кировоградской государственной сельскохозяйственной опытной станции НААН (КГСХОС) (Северная Степь) в 2016–2017 гг. За три года исследований (2015–2017) получены результаты испытания сортов в семи средах. Делянки учетной площадью 10 м2 были заложены в трёхкратной повторности методом полных рендомизированных блоков, в соответствии с общепринятыми методиками. Статистический анализ экспериментальных данных проведен с использованием компьютерных программ Excel 2010 и Statistica 8.0. Для наглядной интерпретации взаимодействия «генотип–среда» использована GGE biplot модель.Результаты. Дисперсионный анализ данных урожайности засвидетельствовал достоверные вклады в общую вариацию среды – 64,64%, генотипа – 14,90% и их взаимодействия – 20,46%. Самой большой дифференцирующей способностью (информативностью) характеризировались условия МИП в 2016 г., наименьшей – условия КГСХОС в 2017 г. Наиболее репрезентативными были условия МИП в 2017 г. и НСХОС в 2016 г., наименее – в КГСХОС (2016 г.). Наиболее отдаленными между собой были условия МИП и КГСХОС в 2016 г. Визуализация GGE biplot «кто-где-победил» позволила разделить среды на два секторы: первый – условия МИП 2015–2017 гг. и НСОС 2016–2017 гг., второй – условия КГСХОС 2016–2017 гг. В первом секторе существенно превалировал сорт ‘МИП Мирный’, во втором – ‘Скарб’. Дифференцированы и выделены сорта ячменя ярового с наиболее оптимальным уровнем проявления урожайности в средах, наиболее близких к гипотетическому «идеальному» генотипу GGE biplot модели – ‘МИП Мирный’, ‘МИП Богун’, ‘Талисман Мироновский’, ‘МИП Азарт’, ‘Доказ’, ‘Пан’. Выводы. Моделирование многосредовых сортоиспытаний путем комбинирования контрастных по гидротермическим режимам годов и различных экологических условий с интерпретацией результатов исследований в соответствии с современными статистическо-графическими методами способствует более детальной характеристике взаимодействия «генотип–среда», ранжированию и выделению перспективных генотипов. | Purpose. To define the regularities of yield level for spring barley varieties in “genotype–environment” interaction when testing in different ecological zones environments of Ukraine and identify genotypes with increased adaptive potential. Methods. As object of the research there were 36 spring barley varieties of domestic and foreign breeding. Varieties were tested at the V. M. Remeslo Myronivka Institute of Wheat of NAAS (MIW) (the Central Forest-Steppe) in 2015–2017, at Nosivka Plant Breeding Experimental Station of the V. M. Remeslo Myronivka Institute of Wheat of NAAS (NPBES) (Polissia) in 2016–2017 and at Kirovohrad State Agricultural Experimental Station of NAAS (KSAES) (the Northern Steppe) in 2016–2017. During three years of the investigation (2015–2017), the results of varieties testing in seven environments have been obtained. Plots with discount area of 10 m2 were laid out with three replications by the method of full randomized blocks, in accordance with conventional methods. Statistical analysis of experimental data was performed using Excel 2010 and Statistica 8.0 software. To interpret visually “genotype-environment” interaction the GGE biplot model was used. Results. The ANOVA of yield data showed reliable contributions into the total variation of environment (64.64%), genotype (14.90%), and their interaction (20.46%). Environmental conditions of MIW in 2016 were characterized with the highest discriminative fineness (informativeness), while KSAES in 2017 were characterized with the lowest one. Environmental conditions of both MIW in 2017 and NPBES in 2016 were the most representative; conditions of KSAES in 2016 were the least representative. The conditions of MIW and KSSGDS in 2016 were the most distant against each other. The GGE biplot “who-won-where” vizualization allowed to divide the environments in two sectors: the first – conditions of MIW 2015–2017 and NPBES 2016–2017, the second – conditions of KSAES 2016–2017. The variety ‘MIP Myrnyi’ had a significant advantage in the first sector, while the variety ‘Skarb’ had it in the second one. The varieties of spring barley ‘MIP Myrnyi’, ‘MIP Bohun’, ‘Talisman Myronivskyi’, ‘MIP Azart’, ‘Dokaz’, ‘Pan’ have been differen­tiated and defined as those with the optimal level of yield in environments being the closest to hypothetical “ideal” genotype of the GGE biplot model. Conclusions. Modelling of integrated variety testing by combining years being contrast in hydrothermal regime and different ecological conditions with interpretation of the investigation results using modern statistical and graphical method contributes to more detailed characterization of the “genotype–environment” interaction, ranking and identifying of prospecting genotypes. | Мета. Виявити закономірності рівня прояву врожайності сортів ячменю ярого у взаємодії «генотип–середовище» за випробування в різних екологічних зонах України та виділити генотипи з підвищеним адаптивним потенціалом.Методи. Об’єкт досліджень – 36 сортів ячменю ярого вітчизняної та зарубіжної селекції. Сортовипробування проведено в Миронівському інституті пшениці імені В. М. Ремесла НААН України (МІП) (Центральний Лісостеп) у 2015–2017 рр., на Носівській селекційно-дослідній станції МІП (НСДС) (Полісся) та на Кіровоградській державній сільськогосподарській дослідній станції НААН (КДСГДС) (Північний Степ) у 2016–2017 рр. За три роки досліджень (2015–2017) отримано результати випробування сортів у семи середовищах. Ділянки обліковою площею 10 м2 закладали у триразовій повторності методом повних рендомізованих блоків, відповідно до загальноприйнятих методик. Статистичний аналіз експериментальних даних проведено з використанням комп’ютерних програм Excel 2010 і Statistica 8.0. Для наочної інтерпретації взаємодії «генотип–середовище» використано GGE biplot модель.Результати. Дисперсійний аналіз даних урожайності засвідчив достовірні внески у загальну варіацію середовища – 64,64%, гено­типу – 14,90% та їх взаємодії – 20,46%. Найвищою диференціювальною здатністю (інформативністю) характеризувались умови МІП у 2016 р., найнижчою – умови КДСГДС у 2017 р. Найбільш репрезентативними були умови МІП у 2017 р. і НСДС у 2016 р., найменш репрезентативними – у КДСГДС (2016 р.). Найвіддаленішими між собою були умови МІП та KДСГДС у 2016 р. Візуалізація GGE biplot «хто-де-переміг» дала змогу розподілити середовища на два сектори: перший – умови МІП 2015–2017 рр. і НСДС 2016–2017 рр., другий – умови КДСГДС 2016–2017 рр. У першому секторі суттєву перевагу мав сорт ‘МІП Мирний’, у другому – сорт ‘Скарб’. Диференційовано й виділено сорти ячменю ярого з оптимальним рівнем прояву врожайності в середовищах, найближчих до гіпотетичного «ідеального» генотипу GGE biplot моделі – ‘МІП Мирний’, ‘МІП Богун’, ‘Талісман Миронівський’, ‘МІП Азарт’, ‘Доказ’, ‘Пан’.Висновки. Моделювання багатосередовищних сортовипробувань шляхом комбінування контрастних за гідротермічним режимом років і різних екологічних умов з інтерпретацією результатів досліджень за сучасними статистично-графічними методами сприяє детальнішій характеристиці взаємодії «генотип–середовище», ранжируванню і виділенню перспективних генотипів.