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Hazelnut breeding in the National Dendrological Park “Sofiyivka” of the NAS of Ukraine 全文
2017
І. С. Косенко | А. І. Опалко | О. А. Балабак | О. А. Опалко | А. В. Балабак
Hazelnut breeding in the National Dendrological Park “Sofiyivka” of the NAS of Ukraine 全文
2017
І. С. Косенко | А. І. Опалко | О. А. Балабак | О. А. Опалко | А. В. Балабак
Purpose. To evaluate hazelnut cultivars, species and hybrids from the genetic collection of Corylus spp. in the National Dendrological Park “Sofiyivka” of the NAS of Ukraine for the complex of economic characters. An attempt has been made to analyze the information on Corylus spp. identity, taxonomy and description, dissemination and ecological requirements of the species, possibilities to use the genetic potential for developing new cultivars. Methods. The value of the Corylus spp. collection representatives was investigated using conventional testing procedures. For summarizing information concerning phylogenetic reconstruction of the Corylus L. genus and hazelnut, a number of scientific publications to be proposed for discussion was analyzed. The oil content in hazelnut kernels and the fatty acid composition was determined using official methods. Results. The best samples of hazelnut genetic collection were included into the broad hybridization programme, and C. chinensis Franch. representatives as well. A number of hybrid seedlings was obtained including new hazelnut cultivars ‘Sofiyivsky 1’, ‘Sofiyivsky 2’ and ‘Sofiyivsky 15’ which were characterized by spherical or almost spherical fruits, high winter hardiness and drought resistance, as well as the absence of rhythmicity in fruiting. Conclusions. The collection of varieties, forms, cultivars and species of the Corylus L. genus created during the last years can be the base for hazelnut breeding in Ukraine.
显示更多 [+] 显示较少 [-]Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combination 全文
2018
Lawrence,Daniel | Holland,Leslie | Nouri,Mohamed | Travadon,Renaud | Abramians,Ara | Michailides,Themis | Trouillas,Florent
Cytospora species are destructive canker and dieback pathogens of woody hosts in natural and agroecosystems around the world. In this genus, molecular identification has been limited due to the paucity of multi-locus sequence typing studies and the lack of sequence data from type specimens in public repositories, stalling robust phylogenetic reconstructions. In most cases a morphological species concept could not be applied due to the plasticity of characters and significant overlap of morphological features such as spore dimensions and fruiting body characters. In this study, we employed a molecular phylogenetic framework with the inclusion of four nuclear loci (ITS, translation elongation factor 1-alpha, actin, and beta-tubulin) to unveil the biodiversity and taxonomy of this understudied important genus of plant pathogens. Phylogenetic inferences based on 150 Californian isolates revealed 15 Cytospora species associated with branch and twig cankers and dieback of almond, apricot, cherry, cottonwood, olive, peach, pistachio, plum, pomegranate, and walnut trees in California. Of the 15 species recovered in this study, 10 are newly described and typified, in addition to one new combination. The pathogenic status of the newly described Cytospora species requires further investigation as most species were associated with severe dieback and decline of diverse and economically important fruit and nut crops in California.
显示更多 [+] 显示较少 [-]Obtaining plant Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis 全文
2017
Гонтаренко, С. М | Лашук, С. О
Obtaining plant Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis 全文
2017
Гонтаренко, С. М | Лашук, С. О
Purpose. To obtain Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis. Methods. Biotechnological procedures, mathematical and statistical analyses. Results. Composition of nutrient medium was developed intended for induction of callusogenesis from Miscanthus seeds with a poor germination and viability of seedlings – Murashige and Skoog (MS) medium was modified for the amount of macroelements (half-dose) that was supplemented with amino acids (300 mg/l of glutamic acid, 50 mg/l of aspartic acid, 5 mg/l of tyrosine, 3 mg/l of arginine, 2 mg/l of hydroxyproline) and plant growth regulators [2,5 mg/l of 2.4D (2.4-Dichlorophenoxyacetic acid), 0,6 mg/l of BAP (6-Benzyl-aminopurine) and 0,3 mg/l of ABA (Abscisic acid)]. Composition of nutrient medium was developed for regeneration of microplants from callus – agar MS medium was modified for the amount of macroelements (half-dose) supplemented with vitamins: 10 mg/l of thiaminum, 1,0 mg/l of pyridoxine, 1,0 mg/l of nicotinic acid (by White), 1,0 mg/l of ascorbic acid, 250 mg/l of glutamic acid, 2,0 mg/l of BAP, 0,3 mg/l of NAA (Naphthaleneacetic acid). On this medium, 100% regeneration of M. sacchariflorus (Maxim.) Hack and 50% regeneration of M. sinensis Andersson was obtained. Due to media modification aimed at initiating callusogenesis and microplants regeneration, reproduction factor of M. sinensis was increased 20 times at the average, M. sacchariflorus – 35–40 times. Conclusions. Plants of M. sacchariflorus (Maxim.) Hack and M. sinensis Andersson were obtained in vitro culture by initiation of callusogenes and microplants regeneration from the Miscanthus seeds with poor germination and viability on nutrient media of certain composition.
显示更多 [+] 显示较少 [-]Obtaining plant Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis 全文
2017
С. М. Гонтаренко | С. О. Лашук
Purpose. To obtain Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis. Methods. Biotechnological procedures, mathematical and statistical analyses. Results. Composition of nutrient medium was developed intended for induction of callusogenesis from Miscanthus seeds with a poor germination and viability of seedlings – Murashige and Skoog (MS) medium was modified for the amount of macroelements (half-dose) that was supplemented with amino acids (300 mg/l of glutamic acid, 50 mg/l of aspartic acid, 5 mg/l of tyrosine, 3 mg/l of arginine, 2 mg/l of hydroxyproline) and plant growth regulators [2,5 mg/l of 2.4D (2.4-Dichlorophenoxyacetic acid), 0,6 mg/l of BAP (6-Benzyl-aminopurine) and 0,3 mg/l of ABA (Abscisic acid)]. Composition of nutrient medium was developed for regeneration of microplants from callus – agar MS medium was modified for the amount of macroelements (half-dose) supplemented with vitamins: 10 mg/l of thiaminum, 1,0 mg/l of pyridoxine, 1,0 mg/l of nicotinic acid (by White), 1,0 mg/l of ascorbic acid, 250 mg/l of glutamic acid, 2,0 mg/l of BAP, 0,3 mg/l of NAA (Naphthaleneacetic acid). On this medium, 100% regeneration of M. sacchariflorus (Maxim.) Hack and 50% regeneration of M. sinensis Andersson was obtained. Due to media modification aimed at initiating callusogenesis and microplants regeneration, reproduction factor of M. sinensis was increased 20 times at the average, M. sacchariflorus – 35–40 times. Conclusions. Plants of M. sacchariflorus (Maxim.) Hack and M. sinensis Andersson were obtained in vitro culture by initiation of callusogenes and microplants regeneration from the Miscanthus seeds with poor germination and viability on nutrient media of certain composition.
显示更多 [+] 显示较少 [-]The results of hexaploid triticale breeding for winter hardiness 全文
2017
Щипак, Г. В | Матвієць, В. Г | Рябчун, Н. І | Щипак, В. Г
The results of hexaploid triticale breeding for winter hardiness 全文
2017
Щипак, Г. В | Матвієць, В. Г | Рябчун, Н. І | Щипак, В. Г
Purpose. Analysis of the process of hexaploid triticale breeding for winter hardiness by intraspecific hybridization method using systemic ecological tests under contrasting conditions. Methods. Dialectical investigation, field experiments, laboratory testing and statistical evaluation. Results. The results of breeding winter and alternate triticale varieties possessing a complex of valuable traits by the method of intraspecific hybridization of forms of different types of development using systemic ecological tests under contrasting conditions (Forest-Steppe – extremely arid Steppe) and at low temperatures were presented. During the years of research (1980–2005), 18 varieties were developed and transferred to the state testing, 17 of them were registered. Conclusions. Thus, the effective selection of highly productive genotypes with increased and high winter hardiness is possible from populations obtained by crossing hexaploid triticale of different types of development (winter triticale with spring and alternate ones) and contrasting level of frost and winter hardiness. During the period of 1980–2015, medium-tall and dwarf varieties of winter (‘Amfidyploid 256’, ‘Garne’, ‘Kharroza’, ‘Rarytet’, ‘Timofey’, etc.) and alternate (‘Nikanor’, ‘Yaroslava’, ‘Plastun Volynskyi’) triticales were developed. They are superior to the standard varieties of soft winter wheat for the critical temperature of freezing by -0.5...- 2.0 °C, characterized by increased (up to 9–12 t/ha) grain yield of various quality depending on the purpose of use.
显示更多 [+] 显示较少 [-]The results of hexaploid triticale breeding for winter hardiness 全文
2017
Г. В. Щипак | В. Г. Матвієць | Н. І. Рябчун | В. Г. Щипак
Purpose. Analysis of the process of hexaploid triticale breeding for winter hardiness by intraspecific hybridization method using systemic ecological tests under contrasting conditions. Methods. Dialectical investigation, field experiments, laboratory testing and statistical evaluation. Results. The results of breeding winter and alternate triticale varieties possessing a complex of valuable traits by the method of intraspecific hybridization of forms of different types of development using systemic ecological tests under contrasting conditions (Forest-Steppe – extremely arid Steppe) and at low temperatures were presented. During the years of research (1980–2005), 18 varieties were developed and transferred to the state testing, 17 of them were registered. Conclusions. Thus, the effective selection of highly productive genotypes with increased and high winter hardiness is possible from populations obtained by crossing hexaploid triticale of different types of development (winter triticale with spring and alternate ones) and contrasting level of frost and winter hardiness. During the period of 1980–2015, medium-tall and dwarf varieties of winter (‘Amfidyploid 256’, ‘Garne’, ‘Kharroza’, ‘Rarytet’, ‘Timofey’, etc.) and alternate (‘Nikanor’, ‘Yaroslava’, ‘Plastun Volynskyi’) triticales were developed. They are superior to the standard varieties of soft winter wheat for the critical temperature of freezing by -0.5...- 2.0 °C, characterized by increased (up to 9–12 t/ha) grain yield of various quality depending on the purpose of use.
显示更多 [+] 显示较少 [-]Peculiarities in covering the requirements for seed material of sorghum crops 全文
2017
Мельник, С. І | Гаврилюк, М. М | Каражбей, Г. М | Курочка, Н. В | Дудка, Т. В
Peculiarities in covering the requirements for seed material of sorghum crops 全文
2017
Мельник, С. І | Гаврилюк, М. М | Каражбей, Г. М | Курочка, Н. В | Дудка, Т. В
Purpose. To assess the demand for sorghum seed material and sufficiency of domestic seeds. Results. The analysis of the State register for the period of 2002–2012 showed that there was the tendency not only towards increasing quantity of sorghum crops in general but their substitution by hybrids of foreign breeding. During the period from 2002 to 2017, 72 sorghum varieties were entered on the State register in total, among them only 12 varieties were of domestic breeding, the rest 60 was presented by foreign breeding institutions. Investigation results allowed to determine that the production of base and prebase seeds of sorghum in 2010 amounted to 1,3 t, in 2016 was 43 t. During the same period the production of sugar sorghum increased from 0,2 to 12,0 t, grass sorghum – from 4,0 to 83 t. In 2017, requirements of acreage of such crops as grass sorghum and broomcorn were completely satisfied by the amount of grown seeds. At the same time, the need for seeds of sorghum and sugar sorghum can not be covered completely at the expense of domestic varieties reproduction. In 2017, general demand for sorghum seeds was 400,5 t, among which only 42,0 t was of domestic production. The rest demand for seeds will be met at the expense of import of foreign breeding seeds into the country to be grown and prepared for sowing abroad. Conclusions. In the Register of plant varieties suitable for dissemination in Ukraine, there are 72 sorghum varieties among them only 12 varieties were of domestic breeding, that is 17%, as compared to 83% of recommended great sorghum varieties of foreign breeding. In Ukraine, the area occupied by sorghum cultivation was 22,8 thou ha in 2005, up to 2017 it increased to 89,0 thou ha, and accordingly the demand for seeds run up from 102,6 to 400,5 t. The area occupied by the sugar sorghum in 2005 amounted to only 2,6 thou ha, in 2017 – 20,0 thou ha, that accordingly determined increase of demand for seed material from 13,0 to 99,9 t. In 2017, the part of the area where domestic hybrids of sorghum are growing was 10%, hybrids of sugar sorghum – 12%, that was indicative of very poor situation with domestic varieties dissemination.
显示更多 [+] 显示较少 [-]Peculiarities in covering the requirements for seed material of sorghum crops 全文
2017
С. І. Мельник | М. М. Гаврилюк | Г. М. Каражбей | Н. В. Курочка | Т. В. Дудка
Purpose. To assess the demand for sorghum seed material and sufficiency of domestic seeds. Results. The analysis of the State register for the period of 2002–2012 showed that there was the tendency not only towards increasing quantity of sorghum crops in general but their substitution by hybrids of foreign breeding. During the period from 2002 to 2017, 72 sorghum varieties were entered on the State register in total, among them only 12 varieties were of domestic breeding, the rest 60 was presented by foreign breeding institutions. Investigation results allowed to determine that the production of base and prebase seeds of sorghum in 2010 amounted to 1,3 t, in 2016 was 43 t. During the same period the production of sugar sorghum increased from 0,2 to 12,0 t, grass sorghum – from 4,0 to 83 t. In 2017, requirements of acreage of such crops as grass sorghum and broomcorn were completely satisfied by the amount of grown seeds. At the same time, the need for seeds of sorghum and sugar sorghum can not be covered completely at the expense of domestic varieties reproduction. In 2017, general demand for sorghum seeds was 400,5 t, among which only 42,0 t was of domestic production. The rest demand for seeds will be met at the expense of import of foreign breeding seeds into the country to be grown and prepared for sowing abroad. Conclusions. In the Register of plant varieties suitable for dissemination in Ukraine, there are 72 sorghum varieties among them only 12 varieties were of domestic breeding, that is 17%, as compared to 83% of recommended great sorghum varieties of foreign breeding. In Ukraine, the area occupied by sorghum cultivation was 22,8 thou ha in 2005, up to 2017 it increased to 89,0 thou ha, and accordingly the demand for seeds run up from 102,6 to 400,5 t. The area occupied by the sugar sorghum in 2005 amounted to only 2,6 thou ha, in 2017 – 20,0 thou ha, that accordingly determined increase of demand for seed material from 13,0 to 99,9 t. In 2017, the part of the area where domestic hybrids of sorghum are growing was 10%, hybrids of sugar sorghum – 12%, that was indicative of very poor situation with domestic varieties dissemination.
显示更多 [+] 显示较少 [-]Peculiarities in covering the requirements for seed material of sorghum crops | Особливості забезпечення потреби в насіннєвому матеріалі соргових культур | Особенности обеспечения потребности в семенном материале сорговых культур 全文
2017
Мельник, С. І. | Гаврилюк, М. М. | Каражбей, Г. М. | Курочка, Н. В. | Дудка, Т. В.
Цель. Оценить потребность в семенном материале сорговых культур и обеспеченность отечественным семенами.Результаты. По результатам анализа Государственного реестра за 2002–2017 гг., наблюдается тенденция не только к увеличению количества сорговых культур в целом, но и к замещению их гибридами зарубежной селекции. Всего за период с 2002 по 2017 г. в Государственный реестр внесено 72 сорта сорго обыкновенного двухцветного, из них только 12 сортов отечественной селекции, остальные 60 представлены зарубежными селекционными учреждениями. По результатам проведенных исследований установлено, что производство базовых и добазовых семян сорго обыкновенного двухцветного в 2010 г. достигало 1,3 т, в 2016 – 42 т. За аналогичный период производство семян сорго сахарного возросло с 0,2 до 12,0 т, сорго суданского – с 4,0 до 83,0 т. В 2017 г. для обеспечения потребностей посевных площадей таких культур, как сорго суданское и сорго веничное вполне достаточно выращенных семян. В то же время потребность в семенах сорго обыкновенного двухцветного и сорго сахарного не в полной мере может быть обеспечена за счет размножения отечественных сортов. В 2017 г. общая потребность в семенах сорго обыкновенного двухцветного составляет 400,5 т, из которых лишь 42,0 т – отечественного производства. Остальная потребность в семенах будет обеспечена за счет импорта в страну семян зарубежной селекции, в частности выращенного и подготовленного к севу.Выводы. В Реестр сортов растений, пригодных к распространению в Украине, внесены 72 сорта сорго обыкновенного двухцветного, из них только 12 сортов отечественной селекции, то есть 17% по сравнению с 83% рекомендованных сортов сорго двухцветного зарубежной селекции. Площади, занятые в Украине под выращиванием сорго обыкновенного двухцветного, в 2005 г. составляли 22,8 тыс. га, до 2017 г. они выросли до 89,0 тыс. га, соответственно и потребность в семенах – с 102,6 до 400, 5 т. Площади, занятые под сорго сахарным, по состоянию на 2005 г. составляли всего 2,6 тыс. га, в 2017 г. – 20,0 тыс. га, что соответственно и определило повышение потребности в семенном материале с 13,0 до 99,9 т. В 2017 доля площадей, на которых выращивают отечественные гибриды сорго обыкновенного двухцветного, составляет 10%, гибриды сорго сахарного – 12%, что свидетельствует о крайне неудовлетворительной ситуации с распространением отечественных сортов. | Мета. Оцінити потребу в насіннєвому матеріалі соргових культур та забезпеченість вітчизняним насінням.Результати. За результатами аналізу Державного реєстру за 2002–2017 рр., спостерігається тенденція не тільки до збільшення кількості соргових культур загалом, а й до заміщення їх гібридами зарубіжної селекції. Всього за період з 2002 по 2017 р. до Державного реєстру внесено 72 сорти сорго звичайного двокольорового, з них лише 12 сортів вітчизняної селекції, решту 60 представлено зарубіжними селекційними установами. За результатами проведених досліджень визначено, що виробництво базового та добазового насіння сорго звичайного двокольорового в 2010 р. було на рівні 1,3 т, у 2016 р. – 42 т. За аналогічний період виробництво насіння сорго цукрового зросло з 0,2 до 12,0 т, сорго суданського – з 4,0 до 83,0 т. У 2017 р. для забезпечення потреб посівних площ таких культур, як сорго суданське та сорго віникове повністю достатньо вирощеного насіння. Водночас, потреба в насінні сорго звичайного двокольорового та сорго цукрового не повною мірою може бути забезпечена за рахунок розмноження вітчизняних сортів. У 2017 р. загальна потреба в насінні сорго звичайного двокольорового становить 400,5 т, з яких лише 42,0 т – вітчизняного виробництва. Решта потреби в насінні буде забезпечена за рахунок імпорту в країну насіння зарубіжної селекції, зокрема вирощеного та підготовленого до сівби за кордоном.Висновки. У Державному реєстрі сортів рослин, придатних до поширення в Україні, перебуває 72 сорти сорго звичайного двокольорового, з них лише 12 сортів вітчизняної селекції, тобто 17%, порівняно з 83% рекомендованих сортів сорго двокольорового іноземної селекції. Площі, зайняті в Україні під вирощуванням сорго звичайного двокольорового, в 2005 р. становили 22,8 тис. га, до 2017 р. вони зросли до 89,0 тис. га, відповідно й потреба в насінні – з 102,6 до 400,5 т. Площі, зайняті під сорго цукровим, станом на 2005 р., становили всього 2,6 тис. га, в 2017 р. – 20,0 тис. га, що відповідно й визначило підвищення потреби в насіннєвому матеріалі з 13,0 до 99,9 т. У 2017 р. частка площ, на яких вирощують вітчизняні гібриди сорго звичайного двокольорового, становить 10%, гібриди сорго цукрового – 12%, що свідчить про вкрай незадовільну ситуацію з поширенням вітчизняних сортів. | Purpose. To assess the demand for sorghum seed material and sufficiency of domestic seeds.Results. The analysis of the State register for the period of 2002–2012 showed that there was the tendency not only towards increasing quantity of sorghum crops in general but their substitution by hybrids of foreign breeding. During the period from 2002 to 2017, 72 sorghum varieties were entered on the State register in total, among them only 12 varieties were of domestic breeding, the rest 60 was presented by foreign breeding institutions. Investigation results allowed to determine that the production of base and prebase seeds of sorghum in 2010 amounted to 1,3 t, in 2016 was 43 t. During the same period the production of sugar sorghum increased from 0,2 to 12,0 t, grass sorghum – from 4,0 to 83 t. In 2017, requirements of acreage of such crops as grass sorghum and broomcorn were completely satisfied by the amount of grown seeds. At the same time, the need for seeds of sorghum and sugar sorghum can not be covered completely at the expense of domestic varieties reproduction. In 2017, general demand for sorghum seeds was 400,5 t, among which only 42,0 t was of domestic production. The rest demand for seeds will be met at the expense of import of foreign breeding seeds into the country to be grown and prepared for sowing abroad. Conclusions. In the Register of plant varieties suitable for dissemination in Ukraine, there are 72 sorghum varieties among them only 12 varieties were of domestic breeding, that is 17%, as compared to 83% of recommended great sorghum varieties of foreign breeding. In Ukraine, the area occupied by sorghum cultivation was 22,8 thou ha in 2005, up to 2017 it increased to 89,0 thou ha, and accordingly the demand for seeds run up from 102,6 to 400,5 t. The area occupied by the sugar sorghum in 2005 amounted to only 2,6 thou ha, in 2017 – 20,0 thou ha, that accordingly determined increase of demand for seed material from 13,0 to 99,9 t. In 2017, the part of the area where domestic hybrids of sorghum are growing was 10%, hybrids of sugar sorghum – 12%, that was indicative of very poor situation with domestic varieties dissemination.
显示更多 [+] 显示较少 [-]Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture 全文
2017
Пикало, С. В | Дубровна, О. В
Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture 全文
2017
Пикало, С. В | Дубровна, О. В
Purpose. To conduct in vitro screening of different genotypes of winter triticale for resistance to salinity in the shoot apical meristem culture. Methods. Plant tissue culture in vitro, in vitro breeding, statistical analysis. Results. It was found that the increase of sodium chloride concentration from 0.6 to 1.5% resulted in inhibition of the callus culture growth in all genotypes that was indicative of the toxic effect of the stress factor. It turns out that 1.2% sodium chloride concentration allowed to differentiate triticale genotypes for salt tolerance. The line ‘38/1296’ appeared to be the most resistant to salinity stress because under breeding conditions calli of this genotype were characterized by higher morphogenetic potential, had the highest crude mass increase, and plants-regenerants were obtained only from explants of this line after cultivation on the medium containing 1.5% sodium chloride. The ‘ADM 11’ variety was the most sensitive to saline stress as mass necrosis and lack of regenerative ability in its calli were observed under breeding conditions. In the studied forms, genotypic dependence of morphogenesis processes in vitro culture was registered. From the induced calli, plants-regenerants were obtained, and their completion of growing, root development and transfer to in vivo conditions were optimized. Conclusions. Genotypic response to salinity stress in the culture of shoot apical meristems of winter triticale was expressed by various crude mass increase and different morphogenetic potential on exposure to a stress factor. The line ‘38/1296’ can be used as a valuable material for further breeding of winter triticale. The culture of shoot apical meristems is recommended to apply as a test system for screening of triticale genotypes for resistance to salinity stress
显示更多 [+] 显示较少 [-]Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture 全文
2017
С. В. Пикало | О. В. Дубровна
Purpose. To conduct in vitro screening of different genotypes of winter triticale for resistance to salinity in the shoot apical meristem culture. Methods. Plant tissue culture in vitro, in vitro breeding, statistical analysis. Results. It was found that the increase of sodium chloride concentration from 0.6 to 1.5% resulted in inhibition of the callus culture growth in all genotypes that was indicative of the toxic effect of the stress factor. It turns out that 1.2% sodium chloride concentration allowed to differentiate triticale genotypes for salt tolerance. The line ‘38/1296’ appeared to be the most resistant to salinity stress because under breeding conditions calli of this genotype were characterized by higher morphogenetic potential, had the highest crude mass increase, and plants-regenerants were obtained only from explants of this line after cultivation on the medium containing 1.5% sodium chloride. The ‘ADM 11’ variety was the most sensitive to saline stress as mass necrosis and lack of regenerative ability in its calli were observed under breeding conditions. In the studied forms, genotypic dependence of morphogenesis processes in vitro culture was registered. From the induced calli, plants-regenerants were obtained, and their completion of growing, root development and transfer to in vivo conditions were optimized. Conclusions. Genotypic response to salinity stress in the culture of shoot apical meristems of winter triticale was expressed by various crude mass increase and different morphogenetic potential on exposure to a stress factor. The line ‘38/1296’ can be used as a valuable material for further breeding of winter triticale. The culture of shoot apical meristems is recommended to apply as a test system for screening of triticale genotypes for resistance to salinity stress
显示更多 [+] 显示较少 [-]Dynamics of correlation relationship changes in new winter wheat varieties (<em>Triticum aestivum</em> L.) under the influence of environmental factors in the Southern Forest-Steppe zone of Ukraine 全文
2017
Гаврилюк, М. М | Каленич, П. Є
Dynamics of correlation relationship changes in new winter wheat varieties (<em>Triticum aestivum</em> L.) under the influence of environmental factors in the Southern Forest-Steppe zone of Ukraine 全文
2017
Гаврилюк, М. М | Каленич, П. Є
Purpose. To establish correlation dependence of influence of such factors as seeding rate, sowing time and seeding methods on yield indicators, as well as individual response of winter wheat varieties to them in case of the ecological system of seed production, that would allow to form elements of intensive varietal technology and obtain the genetically determined productivity potential of the variety in the future. Methods. Weight measuring was used to determine seed yield; the reliability of the experiment results was identified by statistical evaluation, analysis of variance and regression procedure. Results. During the period of investigation, the weather conditions differed significantly both for the temperature regime and the amount of precipitation, but the stability of grain and seed yield over years was the main requirement to varieties, that allowed to study the intergenotypic correlation relationships of crop yield indices and the dynamics of their changes under the effect of ecological factors. In the course of investigation, winter wheat yield indices were fixed annually from 684 plots, which were grouped and analyzed for factors of influence in order to ensure the complete certainty. Conclusions. Variants of investigation were defined for the certified seed yield and environmental factors (sowing time and seeding method, seeding rate), that had strong positive correlation relationships. Seeding rate and seeding method had the greatest impact on 1000 kernel weight. For tillering coefficient, varieties depending on the variant of the investigation had a negative close correlation relationship, which was greatly affected by seeding rate. For correlation relationships, winter wheat varieties ‘Bohdana’ and ‘Slavna’ appeared to be the most stable, ‘Chorniava’ and ‘Astarta’ were the most plastic.
显示更多 [+] 显示较少 [-]Dynamics of correlation relationship changes in new winter wheat varieties (<em>Triticum aestivum</em> L.) under the influence of environmental factors in the Southern Forest-Steppe zone of Ukraine 全文
2017
М. М. Гаврилюк | П. Є. Каленич
Purpose. To establish correlation dependence of influence of such factors as seeding rate, sowing time and seeding methods on yield indicators, as well as individual response of winter wheat varieties to them in case of the ecological system of seed production, that would allow to form elements of intensive varietal technology and obtain the genetically determined productivity potential of the variety in the future. Methods. Weight measuring was used to determine seed yield; the reliability of the experiment results was identified by statistical evaluation, analysis of variance and regression procedure. Results. During the period of investigation, the weather conditions differed significantly both for the temperature regime and the amount of precipitation, but the stability of grain and seed yield over years was the main requirement to varieties, that allowed to study the intergenotypic correlation relationships of crop yield indices and the dynamics of their changes under the effect of ecological factors. In the course of investigation, winter wheat yield indices were fixed annually from 684 plots, which were grouped and analyzed for factors of influence in order to ensure the complete certainty. Conclusions. Variants of investigation were defined for the certified seed yield and environmental factors (sowing time and seeding method, seeding rate), that had strong positive correlation relationships. Seeding rate and seeding method had the greatest impact on 1000 kernel weight. For tillering coefficient, varieties depending on the variant of the investigation had a negative close correlation relationship, which was greatly affected by seeding rate. For correlation relationships, winter wheat varieties ‘Bohdana’ and ‘Slavna’ appeared to be the most stable, ‘Chorniava’ and ‘Astarta’ were the most plastic.
显示更多 [+] 显示较少 [-]Consumptive qualities of different potato varieties 全文
2017
Фурдига, М. М | Верменко, Ю. Я | Сонець, Т. Д
Consumptive qualities of different potato varieties 全文
2017
Фурдига, М. М | Верменко, Ю. Я | Сонець, Т. Д
Purpose. To summarize results of studying consumptive qualities of different varieties of potato and define basic characteristics which allow to subsume them under specific economic categories. Methods. Field study, laboratory test, summarizing, analytical approach. Results. Potato varieties entered in the State register of plant varieties suitable to dissemination in Ukraine and new ones especially to be bred at the Institute of Potato Growing of NAAS were studied during the period of 2005–2016 for such basic economic characters as consumptive quality of tubers, content of starch, dry matters, protein, sugar, vitamins, carotenoids and mineral substances as well as aminoacids, color of flesh, suitability for industrial manufacturing of potato products and for purpose of technology. Attention was paid to the good prospects to use varieties with purple, blue and red potato tuber flesh with high antioxidant capacity. Potato varieties with above characteristics and their complex combination were defined and described. The requirements of processing industry for potato as a raw material for manufacturing of potato food were given. Conclusions. The major criterion for consumptive qualities of a potato variety and correspondingly division for the commercial use is consumptive quality of tubers, especially content of essential nutrients and their favorable combination, improved taste and cooking quality, high antioxidant capacity, suitability for potato products manufacturing and use for purpose of technology. Potato varieties can be divided for economic purposes into edible, suitable for potato products manufacturing, technical and multipurpose ones.
显示更多 [+] 显示较少 [-]Consumptive qualities of different potato varieties 全文
2017
М. М. Фурдига | Ю. Я. Верменко | Т. Д. Сонець
Purpose. To summarize results of studying consumptive qualities of different varieties of potato and define basic characteristics which allow to subsume them under specific economic categories. Methods. Field study, laboratory test, summarizing, analytical approach. Results. Potato varieties entered in the State register of plant varieties suitable to dissemination in Ukraine and new ones especially to be bred at the Institute of Potato Growing of NAAS were studied during the period of 2005–2016 for such basic economic characters as consumptive quality of tubers, content of starch, dry matters, protein, sugar, vitamins, carotenoids and mineral substances as well as aminoacids, color of flesh, suitability for industrial manufacturing of potato products and for purpose of technology. Attention was paid to the good prospects to use varieties with purple, blue and red potato tuber flesh with high antioxidant capacity. Potato varieties with above characteristics and their complex combination were defined and described. The requirements of processing industry for potato as a raw material for manufacturing of potato food were given. Conclusions. The major criterion for consumptive qualities of a potato variety and correspondingly division for the commercial use is consumptive quality of tubers, especially content of essential nutrients and their favorable combination, improved taste and cooking quality, high antioxidant capacity, suitability for potato products manufacturing and use for purpose of technology. Potato varieties can be divided for economic purposes into edible, suitable for potato products manufacturing, technical and multipurpose ones.
显示更多 [+] 显示较少 [-]Functional features of photosynthetic apparatus of modern high-yielding winter wheat varieties 全文
2016
Моргун, В. В | Стасик, О. О | Кірізій, Д. А | Прядкіна, Г. О
Functional features of photosynthetic apparatus of modern high-yielding winter wheat varieties 全文
2016
Моргун, В. В | Стасик, О. О | Кірізій, Д. А | Прядкіна, Г. О
Purpose. Comparative studies of the photosynthetic apparatus of winter wheat varieties of modern and earlier breeding in the relation to their productivity. Methods. Field, pot experiment, biometrical, gas analysis, analytical and statistical ones. Results. It is found that the modern high-yielding winter wheat varieties have a higher rate of flag leaf photosynthesis during generative period of development, particularly at grain filling period, improved ability to store assimilates in stem and effectively use them later for grain growth. The modern varieties form crop canopy with greater leaf area and chlorophyll indexes and larger photosynthetic capacity and maintain functional activity of photosynthetic apparatus longer at the end of the growing season. Conclusions. The superiority for grain productivity of modern high-yielding wheat varieties, originated from the Institute of Plant Physiology and Genetics, NAS of Ukraine, over varieties released before the “green revolution”, results from increased capacity and durability of functioning the crop photosynthetic apparatus, higher CO2 assimilation rate and optimization of source-sink relations in whole plant.
显示更多 [+] 显示较少 [-]Peculiarities of grain technological indices establishment for coated and naked grain oats (Avena sativa L.) 全文
2013
О. І. Буняк
Oats technological indices have been analyzed. Influence of growing conditions on natural weight, kernel size, and hoodness is established. The study summary indicates essential dependence of natural weight and hoodness and precipitation at the stage of grain formation in coated grain oats varieties. Moisture supply in the period of grain formation essentially influenced the percentage of coated grains formation and natural weight score in naked grain varieties. Morphometric analyzes of oats caryopsis was completed using the method of machine vision. Correlation between technological indices and morphometric characteristics of naked and coated grain of oats was determined. Also, essential dependence of hoodness on 1000 seeds weight and the lack of essential correlation with morphometric characteristics of the calliopsis were established. Calliopsis width essentially influenced the weight of 1000 seeds. Metric indices of naked grain oats calliopsis exposed close correlation with kernel size and natural weight. Weight of 1000 seeds exposed essential varietal distinctness and was determined by genetic peculiarities of varieties
显示更多 [+] 显示较少 [-]Bioinformatic analysis of maize gene encoding starch branching enzyme SBEIIb 全文
2016
Сліщук, Г. І | Жернаков, Т. Ю | Волкова, Н. Е
Bioinformatic analysis of maize gene encoding starch branching enzyme SBEIIb 全文
2016
Сліщук, Г. І | Жернаков, Т. Ю | Волкова, Н. Е
Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, primers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are present only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.
显示更多 [+] 显示较少 [-]Bioinformatic analysis of maize gene encoding starch branching enzyme SBEIIb. 全文
2016
Г. І. Сліщук | Т. Ю. Жернаков | Н. Е. Волкова
Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, primers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are present only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.
显示更多 [+] 显示较少 [-]Bioecological peculiarities of mid-ripening varieties of strawberry (Fragaria ananassa Duch.) in the northern part of the Forest-Steppe zone of Ukraine 全文
2016
Павлюк, В. В | Ковальчук, Н. С | Павлюк, Н. В
Bioecological peculiarities of mid-ripening varieties of strawberry (Fragaria ananassa Duch.) in the northern part of the Forest-Steppe zone of Ukraine 全文
2016
Павлюк, В. В | Ковальчук, Н. С | Павлюк, Н. В
Purpose. Improvement of a range of strawberry varieties based on comprehensive study and introduction of new Ukrainian and West European cultivars. Methods. Cultivars and elite forms were studied using the methods of primary variety studying and state variety testing at three-year strawberry plantations of the Institute of Horticulture of the National Academy of Sciences of Ukraine (the city of Kyiv) and its variety testing stations. Results. It was found that more frequent spring and summer droughts as well as black frosts in winter adversely affected the strawberry productivity. The best varieties were selected among new ones for the complex of traits, they included ‘Elegance’ from the Great Britain, ‘Gercules’ from Ukraine (both are dessert grapes), ‘Elsanta’ from Holland (for universal use), ‘Alba’ from Italia (as raw material). The highly adaptive and productive ‘Istochnik’ and ‘Festyvalna romashka’ varieties (as raw material) are recommended for the north regions. Conclusions. In the context of profound climatic change, high-profitable cultivation of mid-ripening varieties and a strawberry as such in the Northern Forest-Steppe zone is expedient under controlled conditions – in high film tunnels, in hot-houses and under perennial irrigation that already has become the European tendency.
显示更多 [+] 显示较少 [-]Bioecological peculiarities of mid-ripening varieties of strawberry (Fragaria ananassa Duch.) in the northern part of the Forest-Steppe zone of Ukraine 全文
2016
В. В. Павлюк | Н. С. Ковальчук | Н. В. Павлюк
Purpose. Improvement of a range of strawberry varieties based on comprehensive study and introduction of new Ukrainian and West European cultivars. Methods. Cultivars and elite forms were studied using the methods of primary variety studying and state variety testing at three-year strawberry plantations of the Institute of Horticulture of the National Academy of Sciences of Ukraine (the city of Kyiv) and its variety testing stations. Results. It was found that more frequent spring and summer droughts as well as black frosts in winter adversely affected the strawberry productivity. The best varieties were selected among new ones for the complex of traits, they included ‘Elegance’ from the Great Britain, ‘Gercules’ from Ukraine (both are dessert grapes), ‘Elsanta’ from Holland (for universal use), ‘Alba’ from Italia (as raw material). The highly adaptive and productive ‘Istochnik’ and ‘Festyvalna romashka’ varieties (as raw material) are recommended for the north regions. Conclusions. In the context of profound climatic change, high-profitable cultivation of mid-ripening varieties and a strawberry as such in the Northern Forest-Steppe zone is expedient under controlled conditions – in high film tunnels, in hot-houses and under perennial irrigation that already has become the European tendency.
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