Purpose. To study the expression of gus and gfp genes in callus explants of amphidiploid spelt wheat (Triticum spelta L.) after Agrobacterium-mediated genetic transformation. Methods. Winter spelt wheat of ‘Europa’ variety was chosen for transformation. Calli obtained from mature embryos were used as explants. Callus pre-cultivation was carried out on MS nutrient medium (Murashige–Skoog) supplemented with 2 mg/L 2.4-D (2.4-Dichlorophenoxyacetic acid) and 10 mg/L silver nitrate. For genetic transformation, Agrobacterium tumefaciens Conn., strain GV3101 and a genetic construct with reporter genes beta-glucuronidase (GUS) and green fluorescent protein (GFP) were used. Calli were transformed by inoculation with agrobacteria and vacuum infiltration. Then they were co-cultured on MS medium with 2 mg/L 2.4-D and 10 mg/L AgNO3, but without antibiotics. The expression of the gus gene was checked by histochemical and the gfp gene by visual analysis (fluorescence of the GFP protein in UV light). Gfp and gus gene expression levels were evaluated using ImajeJ software. The integration of the gfp and gus genes into the spelt genome was verified by PCR. Results. Genetic transformation of spelt callus explants by inoculation in a nutrient medium with agrobacteria and vacuum infiltration occurred at different frequencies. The level of expression of the gus gene during vacuum infiltration was 4.66 ± 0.74%, with inoculation – 4.00 ± 0.91%; and the gfp gene with vacuum infiltration – 3.66 ± 0.74%, with inoculation – 4.66 ± 1.39%. The level of expression of the gfp gene was higher when using inoculation with agrobacteria, and the gus gene was higher during vacuum infiltration. Using PCR analysis, the integration of the gfp and gus genes into the callus of spelt genome was confirmed. The length of the PCR product with primers for the gus gene was 240 bp, and 717 bp for the gfp gene. Conclusions. The use of vacuum infiltration and inoculation methods for spelt genetic transformation gave different results. The frequency of genetic transformation ranged from 3.66 to 4.66%. Agrobacterium-mediated genetic transformation of amphidiploid spelt wheat allows us to study the expression of gus and gfp reporter genes using callus explants derived from mature embryos

Purpose. To study the expression of gus and gfp genes in callus explants of amphidiploid spelt wheat (Triticum spelta L.) after Agrobacterium-mediated genetic transformation.Methods. Winter spelt wheat of ‘Europa’ variety was chosen for transformation. Calli obtained from mature embryos were used as explants. Callus pre-cultivation was carried out on MS nutrient medium (Murashige–Skoog) supplemented with 2 mg/L 2.4-D (2.4-Dichlorophenoxyacetic acid) and 10 mg/L silver nitrate. For genetic transformation, Agrobacterium tumefaciens Conn., strain GV3101 and a genetic construct with reporter genes beta-glucuronidase (GUS) and green fluorescent protein (GFP) were used. Calli were transformed by inoculation with agrobacteria and vacuum infiltration. Then they were co-cultured on MS medium with 2 mg/L 2.4-D and 10 mg/L AgNO3, but without antibiotics. The expression of the gus gene was checked by histochemical and the gfp gene by visual analysis (fluorescence of the GFP protein in UV light). Gfp and gus gene expression levels were evaluated using ImajeJ software. The integration of the gfp and gus genes into the spelt genome was verified by PCR.Results. Genetic transformation of spelt callus explants by inoculation in a nutrient medium with agrobacteria and vacuum infiltration occurred at different frequencies. The level of expression of the gus gene during vacuum infiltration was 4.66 ± 0.74%, with inoculation – 4.00 ± 0.91%; and the gfp gene with vacuum infiltration – 3.66 ± 0.74%, with inoculation – 4.66 ± 1.39%. The level of expression of the gfp gene was higher when using inoculation with agrobacteria, and the gus gene was higher during vacuum infiltration. Using PCR analysis, the integration of the gfp and gus genes into the callus of spelt genome was confirmed. The length of the PCR product with primers for the gus gene was 240 bp, and 717 bp for the gfp gene.Conclusions. The use of vacuum infiltration and inoculation methods for spelt genetic transformation gave different results. The frequency of genetic transformation ranged from 3.66 to 4.66%. Agrobacterium-mediated genetic transformation of amphidiploid spelt wheat allows us to study the expression of gus and gfp reporter genes using callus explants derived from mature embryos | Цель. Исследовать экспрессию генов gus и gfp в каллюсных эксплантах амфидиплоидной пшеницы спельты (Triticum spelta L.) после Agrobacterium-опосредованной генетической трансформации. Методы. Для трансформации был выбран сорт пшеницы спельты озимой ‘Европа’. В качестве эксплантов использовали каллюсы, полученные из зрелых зародышей. Для прекультивации каллюсов использовали питательную среду МС (Мурасиге–Скуга), дополненную 2 мг/л 2,4-Д (2,4-дихлорфеноксиуксусная кислота) и 10 мг/л нитратом серебра. Для генетической трансформации использовали Agrobacterium tumefaciens Conn., штамм GV3101, и генетическую конструкцию, содержащую репортерные гены gus (ген бета-глюкуронидазы) и gfp (ген зеленого флюоресцентного белка GFP). Каллюсы трансформировали путем инокуляции с агробактериями и вакуумной инфильтрацией. Далее их ко-культивировали на среде МС с 2 мг/л 2,4-Д и 10 мг/л AgNO3, но без антибиотиков. Экспрессию гена gus проверяли с помощью гисто­химического анализа, а гена gfp – визуального (флуоресценция белка GFP в UV свете). Уровни экспрессии генов gfp и gus оценивали с помощью программного обеспечения ImajeJ. Интеграцию генов gfp и gus в геном спельты проверяли методом ПЦР. Результаты. Генетическая трансформация каллюсных эксплантов спельты путём их инокуляции в питательной среде с агробактериями и вакуумной инфильтрацией происходила с разной частотой. Уровень экспрессии гена gus при вакуумной инфильтрации составил 4,66 ± 0,74%, а при инокуляции – 4,00 ± 0,91%, а гена gfp при вакуумной инфильтрации – 3,66 ± 0,74%, а при инокуляции – 4,66 ± 1,39%. Уровень экспресии гена gfp был выше в случае использования инокуляции с агробактериями, а гена gus – при вакуумной инфильтрации. При помощи ПЦР анализа была подтверждена интеграция генов gfp и gus в геном каллюсов спельты. Длина ПЦР продукта с праймерами к гену gus составила 240 п. н., а для гена gfp – 717 п. н. Выводы. Использование методов вакуумной инфильтрации и инокуляции для генетической трансформации спельты дали разные результаты. Частота генетической трансформации колебалась от 3,66 до 4,66%. Agrobacterium-опосредованная генетическая трансформация амфидиплоидной пшеницы спельты позволяет исследовать экспрессию репортерных генов gus и gfp при использовании каллюсных эксплантов, полученных из зрелых зародышей. | Мета. Дослідити експресію генів gus та gfp в калюсних експлантах амфідиплоїдної пшениці спельти (Triticum spelta L.) після Agrobacterium-опосередкованої генетичної трансформації. Методи. Для трансформації було обрано сорт пшениці спельти озимої ‘Європа’. Як експланти використовували калюси, отримані зі зрілих зародків. Прекультивацію калюсів здійснювали на живильному середовищі МС (Мурасіге–Скуга), доповненому 2 мг/л 2,4-Д (2,4-дихлорфеноксиоцтова кислота) та 10 мг/л нітратом срібла. Для генетичної трансформації використовували Agrobacterium tumefaciens Conn., штам GV3101, та генетичну конструкцію з репортерними генами gus (ген бета-глюкуронідази (-glucuronidase)) та gfp (ген зеленого флюоресцентного білка (Green Fluorescent Protein, GFP)). Калюси трансформували шляхом інокуляції з агробактеріями та вакуумною інфільтрацією. Далі їх ко-культивували на середовищі МС із 2 мг/л 2,4-Д та 10 мг/л AgNO3, але без антибіотиків. Експресію гена gus перевіряли за допомогою гістохімічного, а гена gfp – візуального аналізу (флуоресценція білка GFP в УФ світлі). Рівні експресії генів gfp та gus оцінювали за допомогою програмного забезпечення ImajeJ. Інтеграцію генів gfp та gus в геном спельти перевіряли методом ПЛР.Результати. Генетична трансформація калюсних експлан­тів спельти шляхом їхньої інокуляції в живильному середовищі з агробактеріями та вакуумною інфільтрацією відбувалась з різною частотою. Рівень експресії гена gus за вакуумної інфільтрації становив 4,66 ± 0,74%, за інокуляції – 4,00 ± 0,91%; а гена gfp за вакуумної інфільтрації – 3,66 ± 0,74%, за інокуляції – 4,66 ± 1,39%. Рівень експресії гена gfp був вищим у разі використання інокуляції з агробактеріями, а гена gus – при ва­ку­умній інфільтрації. За допомогою ПЛР-аналізу було під­тверджено інтеграцію генів gfp та gus в геном калюсів спельти. Довжина ПЛР продукту із праймерами до гена gus становила 240 п. н., а до гена gfp – 717 п. н.Висновки. Використання методів вакуумної інфільтрації та інокуляції для генетичної трансформації спельти дали різні результати. Частота генетичної трансформації коливалась від 3,66 до 4,66%. Agrobacterium-опосередкована генетична трансформація амфідиплоїдної пшениці спельти дозволяє дослідити експресію репортерних генів gus та gfp за використання калюсних експлантів, отриманих із зрілих зародків.

Purpose. To study the expression of gus and gfp genes in callus explants of amphidiploid spelt wheat (Triticum spelta L.) after Agrobacterium-mediated genetic transformation. Methods. Winter spelt wheat of ‘Europa’ variety was chosen for transformation. Calli obtained from mature embryos were used as explants. Callus pre-cultivation was carried out on MS nutrient medium (Murashige–Skoog) supplemented with 2 mg/L 2.4-D (2.4-Dichlorophenoxyacetic acid) and 10 mg/L silver nitrate. For genetic transformation, Agrobacterium tumefaciens Conn., strain GV3101 and a genetic construct with reporter genes beta-glucuronidase (GUS) and green fluorescent protein (GFP) were used. Calli were transformed by inoculation with agrobacteria and vacuum infiltration. Then they were co-cultured on MS medium with 2 mg/L 2.4-D and 10 mg/L AgNO3, but without antibiotics. The expression of the gus gene was checked by histochemical and the gfp gene by visual analysis (fluorescence of the GFP protein in UV light). Gfp and gus gene expression levels were evaluated using ImajeJ software. The integration of the gfp and gus genes into the spelt genome was verified by PCR. Results. Genetic transformation of spelt callus explants by inoculation in a nutrient medium with agrobacteria and vacuum infiltration occurred at different frequencies. The level of expression of the gus gene during vacuum infiltration was 4.66 ± 0.74%, with inoculation – 4.00 ± 0.91%; and the gfp gene with vacuum infiltration – 3.66 ± 0.74%, with inoculation – 4.66 ± 1.39%. The level of expression of the gfp gene was higher when using inoculation with agrobacteria, and the gus gene was higher during vacuum infiltration. Using PCR analysis, the integration of the gfp and gus genes into the callus of spelt genome was confirmed. The length of the PCR product with primers for the gus gene was 240 bp, and 717 bp for the gfp gene. Conclusions. The use of vacuum infiltration and inoculation methods for spelt genetic transformation gave different results. The frequency of genetic transformation ranged from 3.66 to 4.66%. Agrobacterium-mediated genetic transformation of amphidiploid spelt wheat allows us to study the expression of gus and gfp reporter genes using callus explants derived from mature embryos

Purpose. Evaluate the introduced samples of soft winter wheat from the international nursery Common Bunt-Resistant Nursery (CBUNT-RES) of different ecological and geographical origin in the southern part of the Forest-Steppe zone of Ukraine according to a set of productivity indicators in order to define the most valuable samples and describe them. Methods. During 2016–2019 on the base of Ustymivka Experimental Station for Plant Production of the V. Ya. Yuriev Plant Production Institute of NAAS the authors studied, evaluated and described 75 new soft winter wheat samples of various eco-geographical origins by productivity traits. In the field and laboratory conditions, indicators of yield and productivi­ty were determined: 1000 grains weight, plant height and ear length, the number of spikelets and grains in the ear, weight of ear and grain and early-ripening. Results. Plant material with increased parameters of economic and biological characters was identified. During studies, it was found that ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘91-142A61/KATIA1//GRISET-4’, ‘SAULESKU#44/TR810200//GRISET-4’, ‘ATTILA/BABAX//PASTOR/4/…’ (IU067591) (Turkey), ‘INTENSIVNAYA//PBW343*2/TUKURU’, ‘SANZAR-8/KKTS’ (Mexico) were the high-yielding varieties. The samples of soft winter wheat as ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘INTENSIVNAYA//PBW343*2/…’ (IU067637) (Mexico), ‘ATAY/GALVEZ87/6/TAST/…’ (IU067587), ‘DE9/MERCAN-2’, ‘KRASNODAR/FRTL/6…’ (IU067595), ‘SAULESKU#44/TR810200//GRISET-4’, ‘GANSU-1/3/AUSGS50AT34/…’ (IU067598), ‘ORKINOS-1*2/3/AUS…’ (IU067608), ‘KAMBARA1/ZANDER-17’ , ‘TAM200/KAUZ/4/CHAM6//…’ (IU067612) (Turkey) contained a large grain weight (more than 5.0 g), plant productivity in these samples was rather high due to increased amount of grains and the thousand kernel weight. Conclusions. The introduced soft winter wheat samples of various eco-geographical origins were adapted to the Southern Forest-Steppe and can be recommended as a source material in breeding to increasing productive capacity.

Purpose. Assessment and selection of self-pollinated S5 maize (Zea mays L.) families with high combining ability and wide adaptive capacity obtained on the basis of specially produced hybrids by crossing elite lines of various genetic plasms with the best main agronomic characters. Methods. Field studies, mathematico-statistical evaluation. Results. An amplitude of grain yield ranging from 5.96–10.96 t/ha (x = 8.44 t/ha) in 2013 to 2.67–7.59 t/ha (x = 5.08 t/ha) in 2014 was determined in the course of study of the testcrosses of self-pollinated S5 families. It was found that different response of genotypes of the studied testcrosses to the year conditions significantly affected the average yield level, which decreased in the stressful 2014 by 3.4 t/ha as compared to 2013. The results of the assessment based on the general and specific combining ability of new parent material of mixed germplasm were shown. A significant variability of the estimates of GCA (general combining ability) effects depending on the year conditions was observed in the course of study. A marked difference in the estimates of GCA effects based on the grain yield was revealed. They were persistently high in 19% of the best self-pollinated families in both years, 14% of the families had persistently low estimates of GCA effects, 67% changed their value depending on the genotype and year conditions. Conclusions. 17 best self-pollinated families with persistently high estimates of GCA, 6 families featuring high tolerance to drought and 4 families with persistently high variances of SCA were selected. The selected families will be used as a parent material in selection programs aimed to create new high performance hybrids.

Purpose. To study the economic and biological characteristics and to reveal the genetic potential of various hybrids of Chinese cabbage depending on the climatic zone of cultivation. Methods. In the experiment, hybrids of Chinese cabbage ‘Pioner F1’ (control), ‘Villi F1’, ‘Manoko F1’, ‘Orient Star F1’, ‘Vitimo F1’, ‘Sprinkin F1’, ‘Summer Highland F1’, ‘Suprin F1’, and ‘Richi F1’ were evaluated. The experiment was laid out in a randomized block design with four replications with a single plot area of 21 m2. The container seedlings (40 days old) were planted in the middle of April according to the scheme 70 cm by 25 cm. Results. Having been planted at the same time, seed germination over the studied hybrids was not simultaneous. The first sprouted seeds (4 days after seeding) belonged to hybrids ‘Villi F1’, ‘Manoko F1’, ‘Orient Star F1’, and‘Summer Highland F1’. Seeds of the other hybrids started active germination on the 5–6 days after seeding. The highest yield of the cabbage heads was obtained from hybrids ‘Villi F1’ (31.7 t/ha) and ‘Sprinkin F1’ (28.7 t/ha), which was 10.0 and 7.0 t/ha more than in the control variant. The yield of ‘Summer Highland F1’ was 24.9 t/ha and ‘Suprin F1’ 24.6 t/ha. Under the conditions of unstable soil moisture, hybrids ‘Villi F1’and ‘Sprinkin F1’ appeared the most productive and ensured yield increase of 10.0 t/ha and 7.0 t/ha, respectively, compared to the control; and crop commercial quality was high. The highest percentage of dry matter (DM) content was in ‘Summer Highland F1’ (6.2%) followed by ‘Sprinkin F1’ (5.9%), which was 1.1% and 0.8% more than in the control. There was no significant difference between the values of the total sugars content over the variants. They ranged between 1.7 and 2.1%, which was similar to the control values. The content of nitrates in the cabbage heads of the studied Chinese cabbage hybrids was within the tolerance limit and amounted to 600 mg/kg (raw mass). Conclusions. Phenological observations of plant development and their biometric indices, depending on the varietal characteristics, indicate that under the conditions of unstable soil moisture, hybrids ‘Villi F1’ and ‘Sprinkin F1’ were more yielding and ensured yield increase of 10.0 t/ha and 7.0 t/ha, respectively, compared to the control. The crop commercial quality was high. The long growing season of ‘Richi F1’ (93 days) did not affect the crop quality and yield and demonstrated the lowest productivity compared to the control and the other experiment variants.

Purpose. To study the economic and biological characteristics and to reveal the genetic potential of various hybrids of Chinese cabbage depending on the climatic zone of cultivation. Methods. In the experiment, hybrids of Chinese cabbage ‘Pioner F1’ (control), ‘Villi F1’, ‘Manoko F1’, ‘Orient Star F1’, ‘Vitimo F1’, ‘Sprinkin F1’, ‘Summer Highland F1’, ‘Suprin F1’, and ‘Richi F1’ were evaluated. The experiment was laid out in a randomized block design with four replications with a single plot area of 21 m2. The container seedlings (40 days old) were planted in the middle of April according to the scheme 70 cm by 25 cm. Results. Having been planted at the same time, seed germination over the studied hybrids was not simultaneous. The first sprouted seeds (4 days after seeding) belonged to hybrids ‘Villi F1’, ‘Manoko F1’, ‘Orient Star F1’, and‘Summer Highland F1’. Seeds of the other hybrids started active germination on the 5–6 days after seeding. The highest yield of the cabbage heads was obtained from hybrids ‘Villi F1’ (31.7 t/ha) and ‘Sprinkin F1’ (28.7 t/ha), which was 10.0 and 7.0 t/ha more than in the control variant. The yield of ‘Summer Highland F1’ was 24.9 t/ha and ‘Suprin F1’ 24.6 t/ha. Under the conditions of unstable soil moisture, hybrids ‘Villi F1’and ‘Sprinkin F1’ appeared the most productive and ensured yield increase of 10.0 t/ha and 7.0 t/ha, respectively, compared to the control; and crop commercial quality was high. The highest percentage of dry matter (DM) content was in ‘Summer Highland F1’ (6.2%) followed by ‘Sprinkin F1’ (5.9%), which was 1.1% and 0.8% more than in the control. There was no significant difference between the values of the total sugars content over the variants. They ranged between 1.7 and 2.1%, which was similar to the control values. The content of nitrates in the cabbage heads of the studied Chinese cabbage hybrids was within the tolerance limit and amounted to 600 mg/kg (raw mass). Conclusions. Phenological observations of plant development and their biometric indices, depending on the varietal characteristics, indicate that under the conditions of unstable soil moisture, hybrids ‘Villi F1’ and ‘Sprinkin F1’ were more yielding and ensured yield increase of 10.0 t/ha and 7.0 t/ha, respectively, compared to the control. The crop commercial quality was high. The long growing season of ‘Richi F1’ (93 days) did not affect the crop quality and yield and demonstrated the lowest productivity compared to the control and the other experiment variants.

Цель. Исследовать хозяйственно-биологические особенности и раскрыть генетический потенциал различных гибридов капусты пекинской в зависимости от климатической зоны выращивания. Методы. Вариантами опыта были гибриды капусты пекинской ‘Pioner F1’ (контроль), ‘Vili F1’, ‘Manoko F1’, ‘Orient Star F1’, ‘Vitimo F1’, ‘Sprinkin F1’, ‘Summer Highland F1’, ‘Suprin F1’, ‘Richi F1’. Площадь учетного участка 21 м2. Опыт закладывался в четырех повторениях, исследуемые варианты размещали методом рендомизированных блоков. Кассетную рассаду в возрасте 40 суток высаживали во второй декаде апреля по схеме 70 × 25 см. Результаты. В один срок посева у гибридов всходы появлялись не одновременно и первые отмечены у гибридов ‘Vili F1’, ‘Manoko F1’, ‘Orient Star F1’ и ‘Summer Highland F1’ – на 4 сутки после посева, а в остальных – массовые всходы наблюдались несколько позже, на 5–6 сутки после посева. Самый высокий товарный урожай головок получили у гибридов ‘Vili F1’ (31,7 т/га) и ‘Sprinkin F1’ (28,7 т/га), что на 10,0 и 7,0 т/га больше, чем в контроле. Существенно ниже была урожайность у гибридов ‘Summer Highland F1’ – 24,9 и ‘Suprin F1’ – 24,6 т/га. В условиях неустойчивого увлажнения более урожайными были гиб­риды ‘Vili F1’ и ‘Sprinkin F1’, которые обеспечили прирост урожая к контролю 10,0 и 7,0 т/га, а продукция имела высокое товарное качество. Самый высокий процент сухих растворимых веществ установлен в головках гибридов ‘Summer Highland F1’ (6,2%) и ‘Sprinkin F1’ (5,9%), что на 1,1 и 0,8% больше контроля. По сумме сахаров не отмечено существенной разницы между вариантами, данный показатель был на уровне контроля (1,7–2,1%). Содержание нитратов в головках исследуемых гибридов капусты пекинской было в пределах допустимой нормы 600 мг/кг сырой массы. Выводы. Фенологические наблюдения за развитием растений и их биометрические показатели в зависимости от сортовых особенностей указывают, что в условиях неустойчивого увлажнения более урожайными были гибриды ‘Vili F1’ и ‘Sprinkin F1’, которые обеспечили прирост урожая к контролю 10,0 и 7,0 т/га, а продукция была высокого товарного качества. Длительный период вегетации у гибрида ‘Richi F1’ – 93 суток не повлиял на качество и урожайность культуры и обеспечил низкие показатели относительно контрольного и исследуемых вариантов. | Мета. Дослідити господарсько-біологічні особливості та розкрити генетичний потенціал різних гібридів капусти пекінської залежно від кліматичної зони вирощування.Методи. Варіантами досліду були гібриди капусти пекінської ‘Pioner F1’ (контроль), ‘Vili F1’, ‘Manoko F1’, ‘Orient Star F1’, ‘Vitimo F1’, ‘Sprinkin F1’, ‘Summer Highland F1’, ‘Suprin F1’, ‘Richi F1’. Площа облікової ділянки 21 м2. Дослід закладався в чотирьох повтореннях, досліджувані варіанти розміщували методом рендомізованих блоків. Касетну розсаду віком 40 діб висаджували в другій декаді квітня за схемою 70 × 25 см.Результати. За одного строку сівби у гібридів сходи з’являлись неодночасно і перші відмічено у гібридів ‘Vili F1’, ‘Manoko F1’, ‘Orient Star F1’та ‘Summer Highland F1’ – на 4 добу після сівби, а у решти – масові сходи спостерігали дещо пізніше, на 5–6 добу після сівби. Найвищий товарний врожай головок одержали у гібридів ‘Vili F1’ (31,7 т/га) та ‘Sprinkin F1’ (28,7 т/га), що на 10,0 і 7,0 т/га більше, ніж у контролі. Істотно нижчою була врожайність у гібридів ‘Summer Highland F1’ – 24,9 і ‘Suprin F1’ – 24,6 т/га. В умовах нестійкого зволоження врожайнішими були гібриди ‘Vili F1’та ‘Sprinkin F1’, які забезпечили приріст урожаю до контролю 10,0 і 7,0 т/га, а продукція була високої товарної якості. Найвищий відсоток сухих розчинних речовин був у головках гібридів ‘Summer Highland F1’ (6,2%) і ‘Sprinkin F1’ (5,9%), що на 1,1 і 0,8% більше за контроль. За сумою цукрів не відмічено істотної різниці між варіантами, даний показник був на рівні контролю (1,7–2,1%). Уміст нітратів у головках досліджуваних гібридів капусти пекінської був у межах допустимої норми – 600 мг/кг сирої маси.Висновки. Фенологічні спостереження за розвитком рослин та їхні біометричні показники залежно від сортових особливостей вказують, що в умовах нестійкого зволоження врожайнішими були гібриди ‘Vili F1’та ‘Sprinkin F1’, які забезпечили приріст урожаю до контролю 10,0 і 7,0 т/га, а продукція була високої товарної якості. Тривалий період вегетації у гібриду ‘Richi F1’– 93 доби, не вплинув на якість і врожайність та забезпечив найнижчі показники відносно контрольного і досліджуваних варіантів. | Purpose. To study the economic and biological characteristics and to reveal the genetic potential of various hybrids of Chinese cabbage depending on the climatic zone of cultivation.Methods. In the experiment, hybrids of Chinese cabbage ‘Pioner F1’ (control), ‘Villi F1’, ‘Manoko F1’, ‘Orient Star F1’, ‘Vitimo F1’, ‘Sprinkin F1’, ‘Summer Highland F1’, ‘Suprin F1’, and ‘Richi F1’ were evaluated. The experiment was laid out in a randomized block design with four replications with a single plot area of 21 m2. The container seedlings (40 days old) were planted in the middle of April according to the scheme 70 cm by 25 cm.Results. Having been planted at the same time, seed germination over the studied hybrids was not simultaneous. The first sprouted seeds (4 days after seeding) belonged to hybrids ‘Villi F1’, ‘Manoko F1’, ‘Orient Star F1’, and‘Summer Highland F1’. Seeds of the other hybrids started active germination on the 5–6 days after seeding. The highest yield of the cabbage heads was obtained from hybrids ‘Villi F1’ (31.7 t/ha) and ‘Sprinkin F1’ (28.7 t/ha), which was 10.0 and 7.0 t/ha more than in the control variant. The yield of ‘Summer Highland F1’ was 24.9 t/ha and ‘Suprin F1’ 24.6 t/ha. Under the conditions of unstable soil moisture, hybrids ‘Villi F1’and ‘Sprinkin F1’ appeared the most productive and ensured yield increase of 10.0 t/ha and 7.0 t/ha, respectively, compared to the control; and crop commercial quality was high. The highest percentage of dry matter (DM) content was in ‘Summer Highland F1’ (6.2%) followed by ‘Sprinkin F1’ (5.9%), which was 1.1% and 0.8% more than in the control. There was no significant difference between the values of the total sugars content over the variants. They ranged between 1.7 and 2.1%, which was similar to the control values. The content of nitrates in the cabbage heads of the studied Chinese cabbage hybrids was within the tolerance limit and amounted to 600 mg/kg (raw mass).Conclusions. Phenological observations of plant development and their biometric indices, depending on the varietal characteristics, indicate that under the conditions of unstable soil moisture, hybrids ‘Villi F1’ and ‘Sprinkin F1’ were more yielding and ensured yield increase of 10.0 t/ha and 7.0 t/ha, respectively, compared to the control. The crop commercial quality was high. The long growing season of ‘Richi F1’ (93 days) did not affect the crop quality and yield and demonstrated the lowest productivity compared to the control and the other experiment variants.

Purpose. The adaptation of regenerant plants to environmental conditions is the final stage of micropropagation. According to a number of authors, when in vitro plants are transferred to in vivo non-sterile conditions, a significant percentage of mortality is recorded. In a previous publication, the regenerative capacity of strawberry (Fragaria vesca L.) in vitro tissues on MS culture medium (Murashige & Skoog, 1962) and a regenerants was obtained (Chornobrov O. Yu., 2019). The objective of the study is to develop an optimal protocol of acclimation of in vitro F. vesca plants to in vivo conditions. Methods. Biotechnological and statistical methods of research were applied. For the research ‘Ruiana’ and ‘Zhovte Dyvo’ cultivars were used with in vitro cultivation cycle of 30–35 days. Prepared plants were planted in 0.33 L plastic contai ners, one piece in a mixture of coconut substrate and perlite (3:1). Plants were kept under high relative humidity (85–90%) conditions for 3–5 days, 6–8 days and 10–14 days. The studies were carried out in the Plant Biotechnology Laboratory of SS of NULES of Ukraine “BFRS” during 2019–2020. Results. The duration of Fragaria vesca regenerant plants exposure in conditions of high relative humidity significantly affected adaptation efficiency. The proportion of ‘Ruiana’ and ‘Zhovte Dyvo’ plants adapted to the greenhouse conditions were 47.6 ± 2.5% and 60.0 ± 1.7%, respectively, when the plants were kept for 10–14 days. A significant efficiency of plant adaptation (more than 70%) was obtained under condition of preliminarily exposure the roots of the plants in an auxin solution for 25–30 minutes with daily application of 30% solution of glycerine as foliar spray. The plants adapted to the greenhouse conditions had pigmentation characteristic of the variety, without signs of chlorosis and vitrification. Conclusions. An optimal protocol for in vitro adaptation of F. vesca cultivars to in vivo conditions was developed and viable plants were obtained. Further research will be aimed at studying the growth and development of F. vesca regenerant plants in open ground.

Two cercosporoid species are respectively described from Mexican whorled milkweed (Asclepias fascicularis), and spineless safflower (Carthamus tinctorius) from California. Passalora californica represents a new pathogen on Asclepias fascicularis, while Ramularia cynarae is confirmed on Carthamus tinctorius and Cynara cardunculus (Asteraceae), and an epitype designated. Pathogenicity is also established for both pathogens based on Koch’s postulate.

Мета. Суперсолодка кукурудза містить ген, експресія якого зумовлює підвищення концентрації цукру. Ідентифіковано гени ендосперму, що регулюють підвищення вмісту цукру і зменшення вмісту крохмалю; найефективнішими з них були shrunken2 (sh2), brittle1 (bt), sugary1 (su1) і sugary enhancer1 (se). Рецесивна мутація shrunken2 (sh2) забезпечує високий уміст цукрів у зерні молочно-воскової стиглості порівняно з іншими мутантними формами, що безпосередньо впливає на смакові якості sh2-гібридів, обумовлює широке використання цієї мутації в селекційній роботі і має найбільший комерційний потенціал. Основним завданням селекції є створення нового покоління високопродуктивних гібридів, яке залежить від наявності високоякісного вихідного селекційного матеріалу. Тому метою роботи було оцінити лінії суперсолодкої кукурудзи за морфо-біологічними ознаками, виділити ліпші лінії-донори генетичних ознак, створити високопродуктивні гібриди кукурудзи. Методи. Морфо-біологічні ознаки досліджених ліній оцінювали за Класифікатором-довідником виду Zea mays L. Комплексний аналіз зразків за господарсько-цінними ознаками проводили згідно з Методичними рекомендаціями для польового та лабораторного вивчення генетичних ресурсів кукурудзи. Уміст редукувальних цукрів у зерні досліджених ліній і гібридів кукурудзи визначали за методом Бертрана. Результати. Досліджено морфо-біологічні ознаки суперсолодкої кукурудзи. Істотні відмінності між дослідженими лініями спостережено за тривалістю вегетаційного періоду, кількістю насінин в одному ряді й кількістю рядів зерен у качані. Виділено лінії-джерела господарсько-цінних ознак для практичної селекції. Проведено схрещування і отримано 50 гібридних комбінацій, серед яких виділено 16, які характеризувалися кращими показниками господарських ознак. Висновки. У результаті аналізу основних морфо-біологічних ознак у гібридних комбінаціях цукрової кукурудзи з мутантним геном sh2 виділено джерела селекційно-цінних ознак. За тривалістю вегетаційного періоду всі лінії було розділено на три групи: ранні – 8 ліній, середні – 6 і пізні – 8 ліній. Установлено, що перспективними донорами продуктивності качана були лінії SH-621, SH-234 і SH-936, висоти рослин – SH-234 і SH-936, кількості рядів зерен – SH-234 і SH-113, довжини качана – SH-318 і SH-936. За оцінкою морфо-біологічних ознак виділено ліпші лінії, які були схрещені, отримано низку гібридів. Найкращі з них – ‘Матір Драконів F1’, ‘Юрмала F1’ і ‘Ларус F1’ – передано на державну реєстрацію.

Purpose. Providing information on the formation, composition, maintenance of the collection of Buckwheat (Fagopyrum Mill.) genus at Ustymivka Experimental Station of Plant Production (Poltava region), and areas of its use: introduction, preservation and study of the material, for the set of index, formation of special collections and providing research and education institutions of Ukraine with a valuable original material. Methods. Methodical developments dealing with formation and maintenance of crop collections, methodical literature on the study of buckwheat collection for breeding and agronomic performance. Results. The author represented results of the collection material study for the main breeding and economically valuable traits including yield, productivity, resistance to abiotic and biotic factors of the environment. The results of work are considered aimed on creating special collections of the gene pool – base, trait (for yield and large seeds; for productivity, drought and heat resistance; for suitability for mechanical cultivation), educational and core ones. Conclusions. All formed and registered collections are a valuable base material for solving a number of breeding goals to increase the quantity and improve the quality of plant production.

Purpose. Identification of soft winter wheat varieties and lines from the Plant Production Institute nd. a. V. Ya. Yuryev, NAAS by allelic state of Pina–D1 and Pinb-D1 genes for targeted use in the breeding for high confectionery properties of flour. Methods. Identification of the Pina-D1 and Pinb-D1 genes allelic state was performed by polymerase chain reaction (PCR) using allele-specific primer pairs. Confectionery properties of flour were evaluated by determining the quality indicators: the water absorption capacity (WAC) of the flour, trial baking of cookies and evaluation of its quality. Results. According to the results of PCR analysis, 9 samples had an allelic composition of puroindoline genes (Pina-D1a and Pinb-D1a) characteristic for soft-grained varieties. Flour of the lines 'L137-26-0-2', 'L137-26-0-3' had the best confectionery properties, it had a WAC value less than 55%, cookies diameter 85 mm, cookies height 10 mm, estimation of a surface of cookies 7– 9 points, what meets the requirements for soft-grained wheat. 76% of the samples belonged to hard-grained varieties and had the corresponding alleles of the Pina-D1 or Pinb-D1 genes. In the studied sample, Pina-D1 gene is represented by 2 alleles: Pina-D1a and Pina-D1b. 27 samples had Pina-D1a allele, which also allows them to be used in breeding programs for grain quality when crossed with soft samples, 4 ones had Pina-D1b allele. As to Pinb-D1 gene, all hard grain samples had Pinb-D1b allele, and the 'Erythrospermum S 424-1 / 14' line was heterogeneous for Pinb-D1a / Pinb-D1b. The flour of these samples had typical for hard wheat quality indicators: WAC 68% and more, cookie diameter of 60–72 mm, cookie height of 13–15 mm, the surface evaluation of 1–4 points. Conclusions. The studies allowed to differentiate the breeding material and transfer a soft winter wheat cultivar of a confectionery use 'L137-26-0-3' ('Mazurok') which has an allelic structure of puroindolins genes (Pina-D1a and Pinb-D1a) characteristic for soft-grained varieties and high confectionery flour properties for qualification examination.

Мета. Ідентифікувати за алельним станом гени Pina-D1 і Pinb-D1 сортів та ліній пшениці м’якої озимої селекції Інституту рослинництва ім. В. Я. Юр’єва НААН для цільового використання в селекції на високі кондитерські показники борошна.Методи. Алельний стан генів Pina-D1 і Pinb-D1 ідентифікували методом полімеразної ланцюгової реакції (ПЛР) з використанням алель-специфічних пар праймерів. Кондитерські властивості борошна оцінювали, визначивши показники якості: водопоглинальну здатність борошна (ВПЗ), пробне випікання печива та оцінювання його якості.Результати. За результатами ПЛР-аналізу 9 зразків мали алельний склад генів пуроіндолінів (Pina-D1a і Pinb-D1а), характерний для м'якозерних сортів. Кращим за кондитерськими властивостями було борошно ліній 'L137-26-0-2', 'L137-26-0-3', воно мало показник ВПЗ менший 55%, діаметр печива 85 мм, висоту – 10 мм, оцінку поверхні – 7–9 балів, що відповідало вимогам до м'якозерних пшениць. 76% зразків належали до твердозерних сортів та мали відповідні алелі генів Pina-D1 або Pinb-D1. У дослідженій вибірці ген Pina-D1 був представлений 2 алелями: Pina-D1а та Pina-D1b. 27 зразків мали алель Pina-D1а, що також дозволило використовувати їх в селекційних програмах на якість зерна при схрещуванні зі зразками типу soft, 4 – алель Pina-D1b. За геном Pinb-D1 всі твердозерні зразки мали алель Pinb-D1b, а лінія 'Еритроспермум S 424-1/14' була гетерогенною Pinb-D1а/Pinb-D1b. Борошно цих зразків мало характерні для твердозерної пшениці показники якості: ВПЗ 68% і більше, діаметр печива 60–72 мм, висота – 13–15 мм, оцінка поверхні – 1–4 бали.Висновки. Виконані дослідження дозволили ефективно диференціювати селекційний матеріал і передати на кваліфікаційну експертизу сорт пшениці м'якої озимої кондитерського напряму використання 'L137-26-0-3' ('Мазурок'), який має алельний склад генів пуроіндолінів (Pina-D1a і Pinb-D1а), характерний для м'якозерних сортів, та високі кондитерські властивості борошна. | Цель. Идентифицировать аллельное состояние генов Pina-D1 и Pinb-D1 сортов и линий пшеницы мягкой озимой селекции Института растениеводства им. В. Я. Юрьева НААН для целевого использования в селекции на высокие кондитерские показатели муки. Методы. Аллельное состояние генов Pina-D1 и Pinb-D1 идентифицировали методом полимеразной цепной реакции (ПЦР) с использованием аллель-специфических пар праймеров. Кондитерские свойства муки оценивали, определив показатели качества: водопоглотительную способность муки (ВПС), пробную выпечку печенья и оценку его качества. Результаты. По результатам ПЦР-анализа 9 образцов имели аллельное состояние генов пуроиндолинов, характерное для мягкозёрных сортов – Pina-D1a и Pinb-D1а. Лучшей по кондитерским свойствам была мука линий пшеницы ‘L137-26-0-2’, ‘L137-26-0-3’, она имела показатель ВПС меньше 55%, диаметр печенья 85 мм, высоту – 10 мм, оценка поверхности печенья составляла 7–9 балов, что соответствовало требованиям к мягкозёрным пшеницам. 76% изученных образцов относились к твёрдозёрным сортам и имели соответствующие аллели генов Pina-D1 или Pinb-D1. В опытной выборке образцов ген Pina был представлен 2 аллелями: Pina D1а и Pina D1b. 27 образцов имели аллель Pina D1а, это также позволило использовать их в селекции на качество зерна при скрещивании с сортами типа soft, 4 – аллель Pina D1b. По гену Pinb все твёрдозёрные образцы имели аллель Pinb D1b, а линия ‘Эритроспермум S 424-1/14’ была гетерогенной Pinb D1а/Pinb D1b. Эти образцы имели соответствующие твёрдозёрным пшеницам показатели качества муки: ВПС 68% и выше, диаметр печенья 60–72 мм, высота – 13–15 мм, оценка поверхности – 1–4 балла. Выводы. Проведенные исследования позволили эффективно дифференцировать селекционный материал и передать на квалификационную экспертизу сорт пшеницы мягкой озимой кондитерского направления использования ‘L137-26-0-3’ (‘Мазурок’), который имеет аллельный состав генов пуроиндолинов (Pina-D1a и Pinb-D1а), характерный для мягкозёрных сортов, и высокие кондитерские свойства муки. | Purpose. Identification of soft winter wheat varieties and lines from the Plant Production Institute nd. a. V. Ya. Yuryev, NAAS by allelic state of Pina–D1 and Pinb-D1 genes for targeted use in the breeding for high confectionery properties of flour. Methods. Identification of the Pina-D1 and Pinb-D1 genes allelic state was performed by polymerase chain reaction (PCR) using allele-specific primer pairs. Confectionery properties of flour were evaluated by determining the quality indicators: the water absorption capacity (WAC) of the flour, trial baking of cookies and evaluation of its quality. Results. According to the results of PCR analysis, 9 samples had an allelic composition of puroindoline genes (Pina-D1a and Pinb-D1a) characteristic for soft-grained varieties. Flour of the lines 'L137-26-0-2', 'L137-26-0-3' had the best confectionery properties, it had a WAC value less than 55%, cookies diameter 85 mm, cookies height 10 mm, estimation of a surface of cookies 7– 9 points, what meets the requirements for soft-grained wheat. 76% of the samples belonged to hard-grained varieties and had the corresponding alleles of the Pina-D1 or Pinb-D1 genes. In the studied sample, Pina-D1 gene is represented by 2 alleles: Pina-D1a and Pina-D1b. 27 samples had Pina-D1a allele, which also allows them to be used in breeding programs for grain quality when crossed with soft samples, 4 ones had Pina-D1b allele. As to Pinb-D1 gene, all hard grain samples had Pinb-D1b allele, and the 'Erythrospermum S 424-1 / 14' line was heterogeneous for Pinb-D1a / Pinb-D1b. The flour of these samples had typical for hard wheat quality indicators: WAC 68% and more, cookie diameter of 60–72 mm, cookie height of 13–15 mm, the surface evaluation of 1–4 points.Conclusions. The studies allowed to differentiate the breeding material  and transfer a soft winter wheat cultivar of a confectionery use 'L137-26-0-3' ('Mazurok') which has an allelic structure of puroindolins genes (Pina-D1a and Pinb-D1a) characteristic for soft-grained varieties and high confectionery flour properties for qualification examination.

Abstract The genus Cercospora includes many important plant pathogens that are commonly associated with leaf spot diseases on a wide range of cultivated and wild plant species. Due to the lack of useful morphological features and high levels of intraspecific variation, host plant association has long been a decisive criterion for species delimitation in Cercospora. Because several taxa have broader host ranges, reliance on host data in Cercospora taxonomy has proven problematic. Recent studies have revealed multi-gene DNA sequence data to be highly informative for species identification in Cercospora, especially when used in a concatenated alignment. In spite of this approach, however, several species complexes remained unresolved as no single gene proved informative enough to act as DNA barcoding locus for the genus. Therefore, the aims of the present study were firstly to improve species delimitation in the genus Cercospora by testing additional genes and primers on a broad set of species, and secondly to find the best DNA barcoding gene(s) for species delimitation. Novel primers were developed for tub2 and rpb2 to supplement previously published primers for these loci. To this end, 145 Cercospora isolates from the Iranian mycobiota together with 25 additional reference isolates preserved in the Westerdijk Fungal Biodiversity Institute were subjected to an eight-gene (ITS, tef1, actA, cmdA, his3, tub2, rpb2 and gapdh) analysis. Results from this study provided new insights into DNA barcoding in Cercospora, and revealed gapdh to be a promising gene for species delimitation when supplemented with cmdA, tef1 and tub2. The robust eight-gene phylogeny revealed several novel clades within the existing Cercospora species complexes, such as C. apii, C. armoraciae, C. beticola, C. cf. flagellaris and Cercospora sp. G. The C. apii s. lat. isolates are distributed over three clades, namely C. apii s. str, C. plantaginis and C. uwebrauniana sp. nov. The C. armoraciae s. lat. isolates are distributed over two clades, C. armoraciae s. str. and C. bizzozeriana. The C. beticola s. lat. isolates are distributed over two clades, namely C. beticola s. str. and C. gamsiana, which is newly described.

Purpose. To determine the efficiency of growing soybean varieties ‘Ustia’, ‘Cordoba’, ‘Estafeta’ depending on the influence of microfertilizers and growth regulators. Methods. Field, laboratory. Results. The article presents the results of studying the efficiency of growing different varieties of soybeans. It was found that in the cultivar ‘Ustia’ the best values of assimilation of photosynthetically active radiation were obtained for the applications of Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) with growth regulator Radostym, and Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) + in the flowering phase (0.25 l/ha) with two studied growth regulators. Similar patterns of the utilization rate of photosynthetic radiation were obtained for the ‘Cordoba’ and ‘Estafeta’ varieties. According to the indicators of the energy balance, the complex use of micronutrient fertilizers and growth regulators in any case pays off with an increase in the yield, which means it is energetically expedient. The maximum coefficients of energy efficiency in the ‘Ustia’, ‘Cordoba’ and ‘Estafeta’ varieties were obtained with the introduction of micronutrient fertilizers Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) + in the flowering phase (0.25 l/ha) and growth regulators Biosyl and Radostym. However, the use of growth regulators separately does not always provide an economic return on investment. In particular, in the variant without Yara Vita Mono Molitrak microfertilizer, the ‘Cordoba’ variety received 28 873 UAH/ha of net profit, while the introduction of growth regulators did not pay off, since there was no significant increase in the yield, and with the use of growth regulators Biosyl and Radostym net profit was within 28824–29179 UAH/ha. In general, the use of micronutrient fertilizer Yara Vita Mono Molitrak with growth regulators Radostim and Biosyl ensu­red guaranteed yield increase. Conclusions. When gro­wing ‘Ustia’, ‘Cordoba’ and ‘Estafeta’ varieties from an energy point of view, it is better to use Yara Vita Mono Molitrak microfertilizer in the budding phase (0.25 l/ha) with the growth regulator Radostym. Under these conditions, the obtained energy efficiency ratio was 3.24; 3.41 and 3.45, and the profit was 31503, 34072 and 34649 UAH/ha, respectively

The trend for development and spreading of diseases and pests of blackcurrant at Orel region in the conditions of extreme drought in 2010 has been detected. Abnormal weather conditions of the summer and the lack of precipitation limited both growth of host plant and disease spreading. At the background of extreme conditions of 2010 the crop phenophases shifted to earlier dates. The awakening of wintering pathogen structures began in the classic dates of the spring, although in summer their biological cycle went off rapidly. Due to the lack of moisture the disease pathogens failed to fully realize their potential of development and spreading. A favorable aspect of the extreme conditions of vegetation period, the reducing of the protective measures against diseases, is to be admitted. Abnormal weather conditions accelerated the pathogen biological cycles and reduced the stock of wintering infection; meanwhile they provoked colonization of the plants by pests. In extremely high air temperature and lasting lack of precipitation the pests number was intensively increasing, exceeding the maliciousness threshold. Due to the above some additional insecticide and acaricide treatments of berry crops were applied. Thus, the vegetation period of 2010 was described as most favorable for the development and spreading of pests and less favorable for disease pathogens.

Purpose. This study was aimed to determine some biochemical parameters and productivity of the gene fund of Silphium L. genus in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine. Methods. Plant raw material investigated at the flowering stage (17 genotypes) and the end of vegetation (20 genotypes) of Silphium spp. 3 species and 4 cultivars studied for the content of nutrients at the flowering. Determination of dry matter, ash, calcium, nitrogenfree extractives conducted according to Hrytsaienko et al. (2003), phosphorus, protein – according to Pochinok (1976), sugars – according to Krishchenko (1983), cellulose – according to Zheng et al. (2018), lipids – according to Zamowski, Suzuki (2004). It was used productivity parameters: yield of above-ground mass, the yield of dry mass, energetic value, yield of energy. Data analyzed statistically. Results. Investigation of nutrients content showed that content of dry matter was in the range of 18.90–29.3%, protein – in the range of 8.88–23.56%, cellulose – 15.10–36.14%, ash – 8.13–12.19%, lipids – 1.83–3.97%; yield of above-ground mass – 45.0–139.0 t/ha, the yield of dry matter – 10.31–36.92 t/ha, energy value – 3933–4249 cal/g, and yield of energy – 43.81–149.27 Gcal/ha. A study of genotypes at the flowering and end of vegetation identified that the content of dry matter for all samples was in a range of 18.38–67.49%, sugars – 2.78–19.0%, ash – 3.93–11.20%, calcium – 1.68–5.99%, phosphorus – 0.14–1.21%, energy value – 3153.36– 3770.28 cal/g. Conclusions. Plant raw material of genotypes of Silphium L. spp. is a valuable source of nutrients. The content of ash, its components, energetic value, and parameters of productivity depending on genotype and stage of growth. The results allow recommending selected Silphium genotypes as perspective energetic crops in Ukraine as well as other countries.

Purpose. This study was aimed to determine some biochemical parameters and productivity of the gene fund of Silphium L. genus in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine.Methods. Plant raw material investigated at the flowering stage (17 genotypes) and the end of vegetation (20 genotypes) of Silphium spp. 3 species and 4 cultivars studied for the content of nutrients at the flowering. Determination of dry matter, ash, calcium, nitrogenfree extractives conducted according to Hrytsaienko et al. (2003), phosphorus, protein – according to Pochinok (1976), sugars – according to Krishchenko (1983), cellulose – according to Zheng et al. (2018), lipids – according to Zamowski, Suzuki (2004). It was used productivity parameters: yield of above-ground mass, the yield of dry mass, energetic value, yield of energy. Data analyzed statistically.Results. Investigation of nutrients content showed that content of dry matter was in the range of 18.90–29.3%, protein – in the range of 8.88–23.56%, cellulose – 15.10–36.14%, ash – 8.13–12.19%, lipids – 1.83–3.97%; yield of above-ground mass – 45.0–139.0 t/ha, the yield of dry matter – 10.31–36.92 t/ha, energy value – 3933–4249 cal/g, and yield of energy – 43.81–149.27 Gcal/ha. A study of genotypes at the flowering and end of vegetation identified that the content of dry matter for all samples was in a range of 18.38–67.49%, sugars – 2.78–19.0%, ash – 3.93–11.20%, calcium – 1.68–5.99%, phosphorus – 0.14–1.21%, energy value – 3153.36– 3770.28 cal/g.Conclusions. Plant raw material of genotypes of Silphium L. spp. is a valuable source of nutrients. The content of ash, its components, energetic value, and parameters of productivity depending on genotype and stage of growth. The results allow recommending selected Silphium genotypes as perspective energetic crops in Ukraine as well as other countries. | Мета. Це дослідження мало на меті визначити деякі біохімічні параметри та продуктивність генофонду рослин роду Silphium L. у Національному ботанічному саду імені M. M. Гришка НАН України.Методи. Рослинна сировина Silphium досліджена у фазі квітування (17 генотипів) та в кінці вегетації (20 генотипів). Уміст поживних речовин під час квітування вивчено у 3 видів та 4 сортів рослин. Визначення вмісту сухої речовини, золи, кальцію, безазотистих екстрактивних речовин проводили за Грицаєнком і співавт. (2003), фосфору, білка – за Починок (1976), цукрів – за Крищенко (1983), целюлози – за Zheng et al. (2018), ліпідів – за Zamowski, Suzuki (2004). Були визначені параметри продуктивності рослин: урожайність наземної маси, вихід сухої маси, енергетична цінність сировини та вихід енергії з одиниці площі. Дані аналізовані статистично.Результати. Дослідження вмісту поживних речовин показало, що вміст сухої речовини знаходився в межах 18,90–29,3%, білка – 8,88–23,56, целюлози – 15,10–36,14, золи – 8,13–12,19, ліпідів – 1,83–3,97%; урожайність наземної маси – 45,0–139,0 т/га, вихід сухої речовини – 10,31–36,92 т/га, енергетична цінність фітосировини – 3933–4249 кал/г, вихід енергії з урожаєм – 43,81–149,27 Гкал/га. Дослідження генотипів у період квітування та в кінці вегетації дало змогу виявити, що вміст сухої речовини в усіх зразках становив 18,38–67,49%, цукрів – 2,78–19,0, золи – 3,93–11,20, кальцію – 1,68–5,99, фосфору – 0,14–1,21%, енергетична цінність – 3153,36–3770,28 кал/г.Висновки. Рослинна сировина генотипів Silphium L. spp. є цінним джерелом поживних речовин. Уміст золи, її компоненти, енергетична цінність та параметри продуктивності залежить від генотипу та періоду росту й розвитку рослин. Результати досліджень дають змогу рекомендувати деякі генотипи Silphium як перспективні енергетичні культури в Україні, а також інших країнах.

Purpose. This study was aimed to determine some biochemical parameters and productivity of the gene fund of Silphium L. genus in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine. Methods. Plant raw material investigated at the flowering stage (17 genotypes) and the end of vegetation (20 genotypes) of Silphium spp. 3 species and 4 cultivars studied for the content of nutrients at the flowering. Determination of dry matter, ash, calcium, nitrogenfree extractives conducted according to Hrytsaienko et al. (2003), phosphorus, protein – according to Pochinok (1976), sugars – according to Krishchenko (1983), cellulose – according to Zheng et al. (2018), lipids – according to Zamowski, Suzuki (2004). It was used productivity parameters: yield of above-ground mass, the yield of dry mass, energetic value, yield of energy. Data analyzed statistically. Results. Investigation of nutrients content showed that content of dry matter was in the range of 18.90–29.3%, protein – in the range of 8.88–23.56%, cellulose – 15.10–36.14%, ash – 8.13–12.19%, lipids – 1.83–3.97%; yield of above-ground mass – 45.0–139.0 t/ha, the yield of dry matter – 10.31–36.92 t/ha, energy value – 3933–4249 cal/g, and yield of energy – 43.81–149.27 Gcal/ha. A study of genotypes at the flowering and end of vegetation identified that the content of dry matter for all samples was in a range of 18.38–67.49%, sugars – 2.78–19.0%, ash – 3.93–11.20%, calcium – 1.68–5.99%, phosphorus – 0.14–1.21%, energy value – 3153.36– 3770.28 cal/g. Conclusions. Plant raw material of genotypes of Silphium L. spp. is a valuable source of nutrients. The content of ash, its components, energetic value, and parameters of productivity depending on genotype and stage of growth. The results allow recommending selected Silphium genotypes as perspective energetic crops in Ukraine as well as other countries.

Purpose. To reveal the peculiarities of leaf area formation and a structure of lentils variety ‘Antonina’ yield depending on the elements of cultivation technology: ino culation with nitrogen-fixing and phosphate-solubilizing microorganisms and the use of foliar growth stimulants. Methods. Field, laboratory. The experimental scheme included inoculation of seeds with nitrogen-fixing microorganisms (Ryzogumin), application of phosphate-solubili zing microorganisms (Polimixobacteryn and Biophosphoryn) into the row zone, and foliar feeding with a growth stimulator (Alga 600). Results. The results of studies of the leaf area formation peculiarities and the structure of lentils yield depending on the influence of cultivation technology elements are given in the article. It was found that the maximum indicators of the leaf area were formed by lentils in the flowering phase, which on average in the experiment was at the level of 37.5 thousand m2/ha, and in the control variant – only 32.0 thousand m2/ha. By inoculating the seeds with Rhyzogumin, applying phosphate-solubilizing biopreparation and foliar feeding, we obtained the maximum parameters of the leaf surface of lentil plants in the experiment. Thus, in the variant of inoculation with Rhyzogumin, application of Biophosphoryn and treatment with Alga 600 lentil plants formed a leaf area of 40.3 thousand m2/ha. However, due to the use of the phosphate-solubilizing biopreparation Polimixobacteryn and Alga 600 on the background of seeds inoculation with Rhyzogumin, the leaf area was formed at the level of 39.9 thousand m2/ha. Conclusions. When the seeds were treated with nitrogen-fixing microorganisms (Rhyzogumin) and phosphate-solubilizing bacteria (Polimixobacteryn and Biophosphoryn), the yield of lentils increased significantly. Thus, in the experimental plots in the variants with Ryzogumin + Polimixobacteryn the yield was – 1.64 t/ha, and in the combination Ryzogumin + Polimixobacteryn + growth stimulator Alga 600 the yield of lentils was – 1.90 t/ha. Seed inoculation had a positive effect on plant height. The best results were obtained when the seeds were treated with Ryzogumin in combination with Biophosphorin and Polimixobacteryn – 44.5 and 44.1 cm, respectively.

Purpose. To reveal the peculiarities of leaf area formation and a structure of lentils variety ‘Antonina’ yield depending on the elements of cultivation technology: ino culation with nitrogen-fixing and phosphate-solubilizing microorganisms and the use of foliar growth stimulants. Methods. Field, laboratory. The experimental scheme included inoculation of seeds with nitrogen-fixing microorganisms (Ryzogumin), application of phosphate-solubili zing microorganisms (Polimixobacteryn and Biophosphoryn) into the row zone, and foliar feeding with a growth stimulator (Alga 600). Results. The results of studies of the leaf area formation peculiarities and the structure of lentils yield depending on the influence of cultivation technology elements are given in the article. It was found that the maximum indicators of the leaf area were formed by lentils in the flowering phase, which on average in the experiment was at the level of 37.5 thousand m2/ha, and in the control variant – only 32.0 thousand m2/ha. By inoculating the seeds with Rhyzogumin, applying phosphate-solubilizing biopreparation and foliar feeding, we obtained the maximum parameters of the leaf surface of lentil plants in the experiment. Thus, in the variant of inoculation with Rhyzogumin, application of Biophosphoryn and treatment with Alga 600 lentil plants formed a leaf area of 40.3 thousand m2/ha. However, due to the use of the phosphate-solubilizing biopreparation Polimixobacteryn and Alga 600 on the background of seeds inoculation with Rhyzogumin, the leaf area was formed at the level of 39.9 thousand m2/ha. Conclusions. When the seeds were treated with nitrogen-fixing microorganisms (Rhyzogumin) and phosphate-solubilizing bacteria (Polimixobacteryn and Biophosphoryn), the yield of lentils increased significantly. Thus, in the experimental plots in the variants with Ryzogumin + Polimixobacteryn the yield was – 1.64 t/ha, and in the combination Ryzogumin + Polimixobacteryn + growth stimulator Alga 600 the yield of lentils was – 1.90 t/ha. Seed inoculation had a positive effect on plant height. The best results were obtained when the seeds were treated with Ryzogumin in combination with Biophosphorin and Polimixobacteryn – 44.5 and 44.1 cm, respectively.

Purpose. To reveal the peculiarities of leaf area formation and a structure of lentils variety ‘Antonina’ yield depending on the elements of cultivation technology: ino culation with nitrogen-fixing and phosphate-solubilizing microorganisms and the use of foliar growth stimulants.Methods. Field, laboratory. The experimental scheme included inoculation of seeds with nitrogen-fixing microorganisms (Ryzogumin), application of phosphate-solubili zing microorganisms (Polimixobacteryn and Biophosphoryn) into the row zone, and foliar feeding with a growth stimulator (Alga 600).Results. The results of studies of the leaf area formation peculiarities and the structure of lentils yield depending on the influence of cultivation technology elements are given in the article. It was found that the maximum indicators of the leaf area were formed by lentils in the flowering phase, which on average in the experiment was at the level of 37.5 thousand m2/ha, and in the control variant – only 32.0 thousand m2/ha. By inoculating the seeds with Rhyzogumin, applying phosphate-solubilizing biopreparation and foliar feeding, we obtained the maximum parameters of the leaf surface of lentil plants in the experiment. Thus, in the variant of inoculation with Rhyzogumin, application of Biophosphoryn and treatment with Alga 600 lentil plants formed a leaf area of 40.3 thousand m2/ha. However, due to the use of the phosphate-solubilizing biopreparation Polimixobacteryn and Alga 600 on the background of seeds inoculation with Rhyzogumin, the leaf area was formed at the level of 39.9 thousand m2/ha.Conclusions. When the seeds were treated with nitrogen-fixing microorganisms (Rhyzogumin) and phosphate-solubilizing bacteria (Polimixobacteryn and Biophosphoryn), the yield of lentils increased significantly. Thus, in the experimental plots in the variants with Ryzogumin + Polimixobacteryn the yield was – 1.64 t/ha, and in the combination Ryzogumin + Polimixobacteryn + growth stimulator Alga 600 the yield of lentils was – 1.90 t/ha. Seed inoculation had a positive effect on plant height. The best results were obtained when the seeds were treated with Ryzogumin in combination with Biophosphorin and Polimixobacteryn – 44.5 and 44.1 cm, respectively. | Мета. Установити особливості формування площі листя та структури врожаю сочевиці ‘Антоніна’ залежно від елементів технології вирощування: інокуляції азотфіксувальними й фосфатмобілізувальними мікроорганізмами та застосування позакоренево стимуляторів росту. Методи. Польові, лабораторні. Схема досліду передбачала інокуляцію насіння азотфіксувальними мікроорганізмами (Ризогумін), унесення в зону рядка фосфатмобілізувальних мікроорганізмів (Поліміксобактерин і Біофосфорин) та позакореневе підживлення стимулятором росту (Альга 600).Результати. У статті наведено результати досліджень щодо вивчення особливостей формування площі листя та структури врожаю сочевиці залежно від впливу елементів технології. У результаті проведених досліджень визначено, що у фазі цвітіння рослин сочевиці утворювали максимальні показники площі листя, яка в середньому по досліду була на рівні 37,5 тис. м2/га, а от на контрольному варіанті – лише 32,0 тис. м2/га. За інокуляції насіння Ризогуміном та внесення фосфатмобілізувальних препаратів і позакореневого підживлення отримали максимальні параметри листкової поверхні рослин сочевиці в досліді. Зокрема, у варіанті інокуляції Ризогуміном, унесення Біофосфорину та оброблення Альга 600 рослини сочевиці формували площу листя 40,3 тис. м2/га. А от у разі застосування на фоні інокуляції насіння Ризогуміном фосфатмобілізувального препарату Поліміксобактерин та Альга 600 була сформована площа листя на рівні 39,9 тис. м2/га.Висновки. За оброблення насіння азотфіксувальними мікроорганізмами (Ризогумін) та фосфатмобілізувальними бактеріями (Поліміксобактерин та Біофосфорин) урожайність сочевиці суттєво збільшувалася. Зокрема, у варіанті застосування Ризогумін + Поліміксобактерин урожайність становила 1,64 т/га, Ризогумін + Поліміксобактерин + стимулятор росту Альга 600 – 1,90 т/га. Інокуляція насіння позитивно вплинула й на висоту рослин. Ліпші результати отримано за оброблення насіння Ризогуміном у поєднанні з Біофосфорином та Поліміксобактерином – 44,5 і 44,1 см відповідно.