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Dissemination of extended-spectrum β-lactamase-producing Escherichia coli carrying mcr-1 among multiple environmental sources in rural China and associated risk to human health
2019
Ji, Xiang | Zheng, Beiwen | Berglund, Björn | Zou, Huiyun | Sun, Qiang | Chi, Xiaohui | Ottoson, Jakob | Li, Xuewen | Lundborg, Cecilia Stålsby | Nilsson, Lennart E.
Antibiotic resistance among gram-negative bacteria is increasingly becoming a problem of global concern. Particularly problematic is the emergence of resistance to last-resort antibiotics such as carbapenems and colistin. The increasing number of reports on the plasmid-mediated colistin resistance gene mcr-1 in isolates worldwide is raising concerns for the future usefulness of this class of antibiotics. Dissemination of mcr-1 is believed to have originated mainly from animal breeding, however, the role of the environment as a transmission source is not yet fully understood. In the current study, 89 extended-spectrum β-lactamase-producing Escherichia coli isolated from 231 samples from different environmental sources in 12 villages in a rural area of Shandong, China, were screened for mcr-1. 17 (19.1%) mcr-1-positive isolates were found from different environmental sources, aggregated in 6 villages. Plasmids of three different Inc-groups carrying mcr-1 were confirmed, indicating that the widespread geographical distribution of mcr-1 in the local area is due to a number of different plasmids. Additionally, almost a third (29.4%) of the isolates carried virulence factors associated to intestinal pathogenic E. coli. These results illustrate the high complexity of the transmission patterns of mcr-1 among different environmental matrices on a local scale and the potential for the environment to facilitate dissemination and emergence of antibiotic-resistant and virulent strains of bacteria.
显示更多 [+] 显示较少 [-]Human impact on atmospheric precipitation in a protected area in Western Poland. Results of long–term observations: Concentrations, deposition and trends
2015
Walna, Barbara
The paper presents the results of long–term measurements of rain chemistry in the woodland area of the Wielkopolski National Park (Western Poland) experiencing some human impact. The ions predominating in precipitation turned out to be those of ammonium and sulfates, which is indicative of human impact related with agriculture and animal breeding as well as with SO2 emissions. In the period 2002–2013 it is possible to identify, for both precipitation and throughfall, statistically significant decreasing trends in the concentrations and deposition of sulfates, ammonium and potassium, and an increasing trend for fluoride. Both bulk deposition and throughfall of N was higher than that of S. The deposition of nitrogen with throughfall was near to the critical load. High levels of acid–forming ions were observed in both, the winter and late–spring seasons, which proves not only the impact of heating but also of the local industry. Notable is the simultaneous occurrence of extremely high levels of acid–forming ions in some precipitation events, which might have many important implications for the soil, water and biota. There was a change in the pattern of atmospheric chemistry observed as a statistically significant downward trend in the rates of ionic concentration equivalents.
显示更多 [+] 显示较少 [-]Airborne Staphylococcus aureus in different environments—a review
2019
Kozajda, Anna | Jeżak, Karolina | Kapsa, Agnieszka
The aim of the literature review was to describe the environments where the presence of airborne Staphylococcus aureus was confirmed and to catalogue the most often used methods and conditions of bioaerosol sampling to identify the bacteria. The basis for searching of studies on S. aureus in the bioaerosol in different environments was PubMed database resources from the years 1990–2019 (May). The review included studies which were carried on in selected environments: hospitals and other health care facilities, large-scale animal breeding, wastewater treatment plants, residential areas, educational institutions, and other public places. The highest concentrations and genetic diversity of identified S. aureus strains, including MRSA (methicillin-resistant S. aureus), have been shown in large-scale animal breeding. The role of the airborne transmission in dissemination of infection caused by these pathogens is empirically confirmed in environmental studies. Commonly available, well-described, and relatively inexpensive methods of sampling, identification, and subtyping guarantee a high reliability of results and allow to obtain fast and verifiable outcomes in environmental studies on air transmission routes of S. aureus strains.
显示更多 [+] 显示较少 [-]Proteomic analysis of ESBL-producing Escherichia coli under bentonite condition
2019
Gao, Lili | Ma, Xiaochun | Hu, Jiaqing | Zhang, Xiaodan | Chai, Tongjie
The dissemination of extended spectrum beta-lactamases (ESBL) genes through gene transfer attracts wide attention. Bentonite is widely used as a feed additive in an animal-breeding environment. In order to obtain a better understanding of the effect of bentonite on Escherichia coli carrying ESBL gene, proteomic analysis was carried out to screen the key proteins. The results showed that a total of 31 proteins were differentially expressed, including 21 up-regulated proteins and 10 down-regulated proteins. These proteins were involved in biosynthetic process, metabolic process, stress response, transport, anaerobic respiration, proteolysis, hydrolase, protein folding, transcription, salvage, and other. The transcriptional level of four genes (mipA, gntY, tldD, and arcA) was in consensus with proteomic results. This study revealed the differentially expressed proteins involved when E. coli was incubated under bentonite and PBS condition, which implied the possibility that bentonite may promote the transfer of ESBL gene between E. coli.
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