Copper oxide nanoparticles (CuO-NP) are used as an efficient alternative to conventional Cu in agriculture and might end up in soils. They show a high toxicity towards cells and microorganisms, but only low toxicity towards soil invertebrates. However, most existing soil ecotoxicological studies were conducted in a sandy reference soil and at test concentrations ≥100 mg Cu/kg soil. Therefore, there is a knowledge gap concerning the effect of soil texture on the toxicity of CuO-NP at lower, more realistic test concentrations. In our study, a sandy reference soil and three loamy soils were spiked with CuO-NP at up to four concentrations, ranging from 5 to 158 mg Cu/kg. We investigated 28-day reproduction as well as weight and Cu content after 14-day bioaccumulation and subsequent 14-day elimination for the springtail Folsomia candida. For the first time we analysed the size distribution of CuO-NP in aqueous test soil extracts by single particle-ICP-MS which revealed that the diameter of CuO-NP significantly increased with increasing concentration, but did not vary between test soils. Negative effects on reproduction were only observed in loamy soils, most pronounced in a loamy-acidic soil (−61%), and they were always strongest at the lowest test concentration. The observed effects were much stronger than reported by other studies performed with sandy soils and higher CuO-NP concentrations. In the same soil and concentration, a moderate impact on growth (−28%) was observed, while Cu elimination from springtails was inhibited. Rather than Cu body concentration, the diameter of the CuO-NP taken up, as well as NP-clay interactions might play a crucial role regarding their toxicity. Our study reports for the first time toxic effects of CuO-NP towards a soil invertebrate at a low, realistic concentration range. The results strongly suggest including lower test concentrations and a range of soil types in nanotoxicity testing.

Plastic particles, which are formed from routinely used plastics and their fragments, have become a new pollutant raising widespread concern about their potential effects. Several studies have been conducted to examine their toxicity, but the effects of nano-sized plastic fragments on freshwater organisms remain largely unclear and need to be further investigated. In this study, larval tilapia were first exposed to 100 nm polystyrene nanoparticles (PS-NPs, 20 mg/L) for seven days and then returned to freshwater without PS-NPs for another seven days in order to determine the toxic effects of PS-NPs at both transcriptomic and metabolomic levels. A total of 203 significantly changed metabolites, and 2,152 differentially expressed unigenes were identified between control and PS-NP treatment groups, control and recovery groups, as well as treatment and recovery groups. Our data suggested that PS-NPs induced abnormal metabolism of glycolipids, energy, and amino acids in tilapia after short-term exposure. Additionally, PS-NPs caused disturbed signaling, as suggested by the transcriptomic results. Different transcriptomic and metabolomic levels between the treatment group and recovery group indicated a persistent impact of PS-NPs on tilapia. The presence of adhesion molecule-related differentially expressed genes (DEGs) suggested that PS-NPs might cause early inflammatory responses. Notably, the detection of chemical stimulus involved in the sensory perception of smell was the most severely impacted biological process. Our work systemically studied the ecotoxicity of nano-sized plastics in aquatic creatures at the molecular and genetic levels, serving as a basis for future investigations on the prevention and treatment of such pollutants.

Rare earth elements and yttrium (REY) are critical elements for a wide range of applications and consumer products. Their growing extraction and use can potentially lead to REY and anthropogenic-REY chemical complexes (ACC-REY) being released in the marine environment, causing concern regarding their potential effects on organisms and ecosystems. Here, we critically review the scientific knowledge on REY sources (geogenic and anthropogenic), factors affecting REY distribution and transfer in the marine environment, as well as accumulation in- and effects on marine biota. Further, we aim to draw the attention to research gaps that warrant further scientific attention to assess the potential risk posed by anthropogenic REY release. Geochemical processes affecting REY mobilisation from natural sources and factors affecting their distribution and transfer across marine compartments are well established, featuring a high variability dependent on local conditions. There is, however, a research gap with respect to evaluating the environmental distribution and fate of REY from anthropogenic sources, particularly regarding ACC-REY, which can have a high persistence in seawater. In addition, data on organismal uptake, accumulation, organ distribution and effects are scarce and at best fragmentary. Particularly, the effects of ACC-REY at organismal and community levels are, so far, not sufficiently studied. To assess the potential risks caused by anthropogenic REY release there is an urgent need to i) harmonise data reporting to promote comparability across studies and environmental matrices, ii) conduct research on transport, fate and behaviour of ACC-REY vs geogenic REY iii) deepen the knowledge on bioavailability, accumulation and effects of ACC-REY and REY mixtures at organismal and community level, which is essential for risk assessment of anthropogenic REY in marine ecosystems.

With the upgrade of wastewater treatment plants (WWTPs) to meet more stringent discharge limits for nutrients, dissolved organic nitrogen (DON) is present at an increasing percentage (up to 85%) in the effluent. Discharged DON is of great environmental concern due to its potentials in stimulating algal growth and forming toxic nitrogenous disinfection by-products (N-DBPs). This article systematically reviewed the characteristics, transformation and ecological impacts of wastewater DON. Proteins, amino acids and humic substances are the abundant DON compounds, but a large fraction (nearly 50%) of DON remains uncharacterized. Biological treatment processes play a dominant role in DON transformation (65–90%), where DON serves as both nutrient and energy sources. Despite of the above progress, critical knowledge gaps remain in DON functional duality, relationship with dissolved inorganic nitrogen (DIN) species, and coupling/decoupling with the dissolved organic carbon (DOC) pool. Development of more rapid and accurate quantification methods, modeling transformation processes, and assessing DON-associated eutrophication and N-DBP formation risks should be given priority in further investigations.

Currently little is known of newer pesticide classes and their occurrence and persistence in recreational lakes. Therefore, the objectives of this study were to (1) assess average pesticide concentrations and loadings entering recreational lakes in three mixed land use watersheds throughout the growing season, (2) evaluate pesticide persistence longitudinally within the lakes, and (3) perform an ecotoxicity assessment. Six sampling campaigns were conducted at three lakes from April through October 2018 to measure the occurrence and persistence during pre, middle, and post growing season. Polar organic chemical integrative samplers (POCIS) were placed in streams near lake inlets and monthly samples were collected for analysis of twelve pesticides. Additional monthly grab water samples were taken at each POCIS location and at the midpoint and outlet of each lake. All pesticide samples were analyzed using liquid chromatography/tandem mass spectrometry (LC/MS/MS) and individual pesticide loading rates were determined. Occurrence and persistence of specific pesticides were significantly different between lakes in varying watershed land uses. Specifically, the recreational lake receiving predominately urban runoff had the highest load of pesticides, likely in the form of biocides, entering the waterbody. Concentrations of imidacloprid exceeded acute and chronic invertebrate levels for 11% and 61% of the sampling periods, respectively, with the recreational lake receiving predominately urban runoff having the most occurrences. Findings from this study are critical for preventing and mitigating potential effects of pesticides, specifically applied as biocides in urban landscapes, from entering and persisting in recreational lakes.

The present study is a literature-based analysis investigating occurrence and the possible consequences of polycyclic aromatic hydrocarbons (PAH) in marine protected areas (MPAs) of Latin America and Caribbean. The approach using overlapping of georeferenced MPA polygons with data compiled from peer-reviewed literature, published during the last 15 years, showed 341 records of PAH in 9 countries. PAH was reported to occur within the boundaries of 36 MPAs located in Argentina, Brazil, Colombia, Mexico, Nicaragua and Uruguay. According to quality guidelines, low to moderate impacts are expected in MPAs categorized in different management classes. Considering sediment samples, 13% of the records presented concentrations enough to cause occasional toxicity. Such level of risk was also seen in Ramsar sites and in Amazonian MPAs. In addition, based on concentrations reported in biota, occasional deleterious effects on organisms from Biosphere Reserves might occur. Diagnostic ratios pointed out petrogenic and pyrolytic processes as PAH predominant sources, and were mainly attributed to the proximity to ports, industries and urban areas. MPAs located in the vicinity of impact-generating areas may be under threat and require government attention and action, mainly through implementation of contamination monitoring programs.

The fragmentation of plastic materials into nanoparticles of less than 1000 nm (secondary nanoplastics) and their possible accumulation in the environment is a recent matter of concern. There are still no suitable standard methods for determining the concentrations and chemical makeup of these particles in aquatic systems and the fate and effect of nanoplastics in the aquatic environment has been little explored, although there has been research using engineered nanoparticles as models. In this review, we give a summary of the (mainly laboratory-based) studies on the influences of nanoplastics. We aim to provide an updated overview of this emerging topic, reviewing the literature mainly from 2018 onwards and considering the effects of nanoplastics on ecosystems, their uptake and transport of polluting molecules, and the challenges that are faced by workers in this area. The review includes 119 references.

Nuclear abnormality (NA) assay in fish has been widely applied for toxicity risk assessment under field and laboratory conditions. The zebrafish (Danio rerio) has become a suitable model system for assessing the NA induced by pollutants. Thus, the current study aimed to summarize and discuss the literature concerning micronucleus (MN) and other NA in zebrafish and its applications in toxicity screening and environmental risk assessment. The data concerning the publication year, pollutant type, experimental design, and type of NA induced by pollutants were summarized. Also, molecular mechanisms that cause NA in zebrafish were discussed. Revised data showed that the MN test in zebrafish has been applied since 1996. The MN was the most frequently NA, but 15 other nuclear alterations were reported in zebrafish, such as notched nuclei, blebbed nuclei, binucleated cell, buds, lobed nuclei, bridges, and kidney-shaped. Several pollutants can induce NA in zebrafish, mainly effluents (mixture of pollutants), agrochemicals, and microplastics. The pollutant-induced NA in zebrafish depends on experimental design (i.e., exposure time, concentration, and exposure condition), developmental stages, cell/tissue type, and the type of pollutant. Besides, research gaps and recommendations for future studies are indicated. Overall, the current study showed that zebrafish is a suitable model to assess pollutant-induced mutagenicity.

Nano metal oxide particles (NMOPs) are widely used in daily life because of their superior performance, and inevitably enter the sewage treatment system. Pollutants in sewage are adsorbed and degraded in wastewater treatment plants (WWTPs) depending on the microbial aggregates of activated sludge system to achieve sewage purification. NMOPs may cause ecotoxicity to the microbial community and metabolism due to their complex chemical behavior, resulting in a potential threat to the safe and steady operation of activated sludge system. It is of great significance to clarify the influencing mechanism of NMOPs on activated sludge system and reduce the risk of WWTPs. Herein, we first introduce the physicochemical behavior of six typical engineering NMOPs including ZnO, TiO₂, CuO, CeO₂, MgO, and MnO₂ in water environment, then highlight the principal mechanisms of NMOPs for activated sludge system. In particular, the performance recovery mechanisms of activated sludge systems in the presence of NMOPs and their future development trends are well documented and discussed extensively. This review can provide a theoretical guidance and technical support for predicting and evaluating the potential threat of NMOPs on activated sludge systems, and promoting the establishment of effective control strategies and performance recovery measures of biological wastewater treatment process under the stress of NMOPs.

Most palm oil mills adopted conventional ponding system, including anaerobic, aerobic, facultative and algae ponds, for the treatment of palm oil mill effluent (POME). Only a few mills installed a bio-polishing plant to treat POME further before its final discharge. The present study aims to determine the quality and toxicity levels of POME final discharge from three different mills by using conventional chemical analyses and fish (Danio rerio) embryo toxicity (FET) test. The effluent derived from mill A which installed with a bio-polishing plant had lower values of BOD, COD and TSS at 45 mg/L, 104 mg/L, and 27 mg/L, respectively. Only mill A nearly met the industrial effluent discharge standard for BOD. In FET test, effluent from mill A recorded low lethality and most of the embryos were malformed after hatching (half-maximal effective concentration (EC50) = 20%). The highest toxicity was observed from the effluent of mill B and all embryos were coagulated after 24 h in samples greater than 75% of effluent (38% of half-maximal lethal concentration (LC50) at 96 h). The embryos in the effluent from mill C recorded high mortality after hatching, and the survivors were malformed after 96 h exposure (LC50 = 26%). Elemental analysis of POME final discharge samples showed Cu, Zn, and Fe concentrations were in the range of 0.10–0.32 mg/L, 0.01–0.99 mg/L, and 0.94–4.54 mg/L, respectively and all values were below the effluent permissible discharge limits. However, the present study found these metals inhibited D. rerio embryonic development at 0.12 mg/L of Cu, and 4.9 mg/L of Fe for 96 h-EC50. The present study found that bio-polishing plant installed in mill A effectively removing pollutants especially BOD and the FET test was a useful method to monitor quality and toxicity of the POME final discharge samples.