Constructed wetland coupled microbial fuel cell (CW-MFC) encompasses both aerobic and anaerobic zones to produce electrical energy while facilitating the oxidative breakdown of pollutants. In this study, we ascertained the effective setup of CW-MFC in order to assess the pollutant removal efficiency and electricity generation. The CW-MFC system was initially filled with textile wastewater. Stainless steel mesh with granular activated carbon as the anode and graphite rods as the cathode were used. Soil and gravel were used as substrates and Canna indica as macrophyte. Over the course of 4 weeks, regular assessments were conducted every 3rd day to monitor the alternations in the wastewater properties. Throughout the treatment phase, the planted CW-MFC system achieved a significant reduction in phosphate, nitrate, BOD, COD, and chloride as compared to the unplanted CW-MFC system. From this study, the results also show that planted CW-MFC produce maximum peak voltage (0.112V) and current (1.12 mA) in comparison to CW-MFC without plants. Consequently, the finding suggests that Canna indica possesses the capacity to treat textile wastewater.

Chicken poultry industry produces a vast amount of feather waste and is often disposed into landfills, creating environmental pollution. Therefore, we explored the valorization of chicken feather waste into lipids and keratinous sludge biomass. This study demonstrates the successful utilization of keratinous sludge biomass as a unique precursor for the facile preparation of novel keratinous sludge biomass-derived carbon-based molybdenum oxide (KSC@MoO₃) nanocomposite material using two-step (hydrothermal and co-pyrolysis) processes. The surface morphology and electrochemical properties of as-prepared nanocomposite material were analyzed using HR-SEM, XRD, XPS, and cyclic voltammetric techniques. KSC@MoO₃ nanocomposite exhibited prominent electrocatalytic behavior to simultaneously determine hydroquinone (HQ) and catechol (CC) in environmental waters. The as-prepared electrochemical sensor showed excellent performance towards the detection of HQ and CC with broad concentration ranges between 0.5–176.5 μM (HQ and CC), and the detection limits achieved were 0.063 μM (HQ) and 0.059 μM (CC). Furthermore, the developed modified electrode has exhibited excellent stability and reproducibility and was also applied to analyze HQ and CC in environmental water samples. Results revealed that chicken feather waste valorization could result in sustainable biomass conversion into a high-value nanomaterial to develop a cost-effective electrochemical environmental monitoring sensor and lipids for biofuel.

The conductive polyurethane/polypyrrole/graphene (CPU/PPy/Gr) particle electrode was prepared by an in-situ oxidative polymerization method and used as particle electrodes to degrade levofloxacin (LEV) in a three-dimensional electrode reactor. The prepared CPU/PPy/Gr electrode was characterized systematically and the effects of initial pH, initial LEV concentration, aeration volume, voltage, and electrolyte concentration on the degradation efficiency were investigated. Results showed that more than 90% LEV was degraded and the energy consumption was 20.12 kWh/g LEV under conditions of pH 7, 6 V voltage, 2.0 L/min aeration volume, 20 mg/L initial LEV concentration, and 7 mM concentration of electrolyte (Na₂SO₄). A possible electrochemical oxidation pathway of LEV by the CPU/PPy/Gr electrode was proposed. In addition, the biotoxicity of LEV and its oxidation products was calculated using ECOSAR (Ecological Structure Activity Relationships) program in EPISuite. Toxicity evaluation using luminescent bacteria showed that the toxicities of some intermediates were higher than the parent compound. But the toxicity of degradation processes for LEV was effective decreasing. A possible reactive mechanism in the three-dimensional reactor was also recommended. In brief, the prepared CPU/PPy/Gr particle electrode constitutes an insight into the promising practical application in the wastewater treatment.

The removal of antibiotics has attracted much attention due to their extremely high adverse impacts on the environment. However, the potential risks of degradation intermediates are seldom reported. In this work, the influence of different factors on the electro-catalytic degradation efficiency of tetracycline hydrochloride (TCH) by the prepared carbon nanotubes/agarose/indium tin oxide (CNTs/AG/ITO) electrode was investigated. Under optimal conditions (10 wt% CNTs dosage, pH = 7), the maximum degradation efficiency for TCH (10 mg L⁻¹) reached up to 96% within 30 min treatment with 4 V potential. Superoxide anions (•O₂⁻) played an important role in the electro-catalytic degradation. Totally 10 degradation intermediates were identified using HPLC-MS/MS, and the degradation pathway was proposed. Toxicities of the parent antibiotic and the identified intermediates were calculated using the ECOSAR (Ecological Structure Activity Relationship) program in EPISuite, and results showed that more toxic intermediates were generated. The maximal chronic toxicity for green algae of the intermediate increased 1439.92 times. Furthermore, antimicrobial activity was further verified by disk agar biocidal tests with Escherichia coli ATCC25922 and higher biotoxicity intermediates compared with parent compounds were confirmed to be formed. Therefore, more attention should be paid on the potential risk of degradation intermediates in the treatment of wastewater containing antibiotics.

The heavy metal ion adsorption performance of birnessite (a layer-structured manganese oxide) can be enhanced by decreasing the Mn average oxidation state (Mn AOS) and dissolution−recrystallization during electrochemical redox reactions. However, the electrochemical adsorption processes of heavy metal ions by tunnel-structured manganese oxides are still enigmatic. Here, tunnel-structured manganese oxides including pyrolusite (2.3 Å × 2.3 Å tunnel), cryptomelane (4.6 Å × 4.6 Å tunnel) and todorokite (6.9 Å × 6.9 Å tunnel) were synthesized, and their electrochemical adsorptions for Cd²⁺ were performed through galvanostatic charge−discharge. The influence of both supporting ion species in the tunnel and tunnel size on the electrochemical adsorption performance was also studied. The adsorption capacity of tunnel-structured manganese oxides for Cd²⁺ was remarkably enhanced by electrochemical redox reactions. Relative to K⁺ in the tunnel of cryptomelane, the supporting ion H⁺ was more favorable to the electrochemical adsorption of Cd²⁺. With increasing initial pH and specific surface area, the electrochemical adsorption capacity of cryptomelane increased. The cryptomelane electrode could be regenerated by galvanostatic charge−discharge in Na₂SO₄ solution. Due to the differences in their tunnel size and supporting ion species, the tunnel-structured manganese oxides follow the order of cryptomelane (192.0 mg g⁻¹) > todorokite (44.8 mg g⁻¹) > pyrolusite (13.5 mg g⁻¹) in their electrochemical adsorption capacities for Cd²⁺.

Herein, we demonstrate a nanocomposite material Eu/ZnO/pPy for enhanced performance in photoelectrocatalytic degradation of chemical warfare agent sulphur mustard (SM) at ambient conditions which is growing concern of the Scientific Community amidst the current climate of terrorism. Eu/ZnO/pPy was electrochemically prepared on Au electrode at ambient conditions and was used for electrocatalytic reductive elimination of chloride from SM and results indicated one electron involvement process for the cleavage of the carbon-chloride bond. Surface morphology of Eu/pPy, ZnO/pPy and Eu/ZnO/pPy composites were characterized by SEM and confirmed the formation of the nanoparticles and nanorods on the modified electrode which leads to provide more surface area for the reductive elimination reaction. The elemental composition, functional groups and phase of materials on the modified electrode were deduced using EDX, Raman spectroscopy and XRD, respectively. Eu/ZnO/pPy/Au electrode was utilized for the photoelectrocatalytic degradation of SM as it exhibit excellent electrocatalytic activity and degradation products were analyzed by GC-MS. In the reductive elimination of SM, the following parameters were deduced (i) heterogeneous rate constant (0.127 s⁻¹), (ii) transfer coefficient (0.32) and (iii) number of electron involved (1.0). The enhanced photoelectrocatalytic capability of this nanocomposite could serve as a novel and promising catalyst in defence and environmental applications.

Bioelectrochemical systems (BESs) have been widely investigated for recalcitrant waste treatment mainly because of their waste removal effectiveness. Electroactive microbes (EMs) have long been thought to contribute to the high effectiveness by interacting with electrodes via electron chains. However, this work demonstrated the dispensable role of EMs for enhanced recalcitrant contamination degradation in BESs. We revealed enhanced p-fluoronitrobenzene (p-FNB) degradation in a BES by observing a defluorination efficiency that was three times higher than that in biodegradation or electrochemical processes. Such an improvement was achieved by the collaborative roles of electrode biofilms and planktonic microbes, as their individual contributions to p-FNB degradation were found to be similarly stimulated by electricity. However, no bioelectrochemical activity was found in either the electrode biofilms or the planktonic microbes during stimulated p-FNB degradation; because no biocatalytically reductive or oxidative turnovers were observed on cyclic voltammetry curves. The non-involvement of EMs was further proven by the similar microbial community evolution for biofilms and planktonic microbes. In summary, we proposed a mechanism for indirect electrical stimulation of microbial metabolism by electrochemically generating the active mediator p-fluoroaniline (p-FA) and further degradation by a sequential combination of electrochemical p-FNB reduction and biological p-FA oxidation by non-EMs.

Enzymatic conductometric biosensor, using immobilized Arthrospira platensis cells on gold interdigitated electrodes, for the detection of pesticides in water, was elaborated. Cholinesterase activity (AChE) was inhibited by pesticides and a variation of the local conductivity was measured after addition of the substrate acetylthiocholine chloride (AChCl). The Michaelis–Menten constant (Km) was evaluated to be 1.8 mM through a calibration curve of AChCl. Inhibition of AChE was observed with paraoxon-methyl, parathion-methyl, triazine and diuron with a detection limit of 10−18 M, 10−20 M, 10−20 M and 10−12 M, respectively and the half maximal inhibitory concentration (IC50) was determined at 10−16 M, 10−20 M, 10−18 M and 10−06 M, respectively. An important decrease of response time τ90% was recorded for AChE response towards AChCl after 30 min cell exposure to pesticides. Scanning electron microscopy images revealed a degradation of the cell surface in presence of pesticides at 10−06 M.

Membrane filtration is commonly performed for solid–liquid separation of aqueous solutions prior to trace metal analysis and when assessing “dissolved” metal fractions. Potential artifacts induced by filtration such as contamination and/or adsorption of metals within the membrane have been investigated for different membrane materials, metals, applied pressures and pre-cleaning steps. Measurements have been conducted on aqueous solutions including well-defined metal standards, ultrapure water, and on runoff water from corroded samples. Filtration using both non-cleaned and pre-cleaned filters revealed contamination and adsorption effects, in particular pronounced for zinc, evident for copper but non-significant for nickel. The results clearly show these artifacts to be non-systematic both for non-cleaned and pre-cleaned membranes. The applied pressure was of minor importance. Measurements of the labile fraction by means of stripping voltammetry clearly elucidate that membrane filtration followed by total metal analysis cannot accurately assess the labile or the dissolved metal fraction.

Fluoride mediated disruption of sex steroid hormones has been demonstrated in animals. However, evidence from humans was limited and contradictory, especially for children and adolescents. Based on data of the National Health and Nutrition Survey (NHANES) 2013–2016, a total of 3392 subjects aged 6–19 years were analyzed in this cross-sectional study. Both plasma and water fluoride levels were quantified electrometrically using the ion-specific electrode. Sex steroid hormones of total testosterone, estradiol and sex hormone-binding globulin (SHBG) were tested in serum. Percent changes and 95% confidence intervals (CIs) in sex steroid hormones associated with tertiles of fluoride levels (setting the first as reference) were estimated using adjusted linear regression models by stratification of gender and age. Compared with subjects at the first tertile of plasma fluoride, percent changes (95% CIs) in testosterone were −8.08% (−17.36%, 2.25%) and −21.65% (−30.44%, −11.75%) for the second and third tertiles, respectively (P ₜᵣₑₙd <0.001). Male adolescents at the third tertile of plasma fluoride had decreased levels of testosterone (percent change = −21.09%, 95% CIs = −36.61% to −1.77%). Similar inverse associations were also found when investigating the relationships between plasma fluoride and estradiol. Besides, the data indicated decreased levels of SHBG associated with water and plasma fluoride among the male adolescents (percent change of the third tertile = −9.39%, 95% CIs = −17.25% to −0.78%) and female children (percent change of the second tertile = −10.78%, 95% CIs = −17.55% to −3.45%), respectively. The data indicated gender- and age-specific inverse associations of fluoride in plasma and water with sex steroid hormones of total testosterone, estradiol and SHBG in U.S. children and adolescents. Prospective cohort studies are warranted to confirm the causality.