Increase in advanced electronic technology leads to environmental issues related with its disposal. Electronic waste i.e., video card and random access memory were used for studying extraction of precious metals using Paenibacillus sp. Metal contaminated soil was used for the isolation of exopolysaccharide producing strains. The isolate was identified as Paenibacillus sp. based on morphological, biochemical tests and 16S rRNA sequencing. Metal content analysis of soil and e-waste was carried out using X-ray Fluorescence spectroscopy. The vanadium element was more in the soil sample which was 0.487 mg/g and in electronic waste sample copper content was more which was 250 mg/g. Paenibacillus sp. produced capsule which was observed under bright, dark field and phase contrast microscope. Scanning electron microscopy was done for the study of morphological changes of exopolysaccharide producing Paenibacillus sp. in chitin broth and on chitin agar medium with and without e-waste. The Fourier Transform Infrared Spectroscopy analysis of exopolysaccharide produced by Paenibacillus sp. grown on chitin agar and chitin agar with e-waste showed presence of different functional groups. The one step and two step bioleaching experiments were carried out for testing efficacy of biomass on metal leaching. Paenibacillus sp. showed its potential for the extraction of precious metals viz., gold, silver and copper from electronic waste. Paenibacillus sp. recovered gold (0.001%), cadmium (45%), copper (50%), iron (46%), manganese (88%), palladium (56.9%) and zinc (87.12%) by two step fermentation. The study is useful for the bioleaching of precious metals from electronic waste.

In this study, Bacillus sp. strain AVPP64 was isolated from diuron-contaminated soil. It showed 4-nitroaniline (4-NA) degradation, pesticide tolerance, and self-nutrient integration via nitrogen (N)-fixation and phosphate (P)-solubilization. The rate constant (k) and half-life period (t₁/₂) of 4-NA degradation in the aqueous medium inoculated with strain AVPP64 were observed to be 0.445 d⁻¹ and 1.55 d, respectively. Nevertheless, in the presence of chlorpyrifos, profenofos, atrazine and diuron pesticides, strain AVPP64 degraded 4-NA with t₁/₂ values of 2.55 d, 2.26 d, 2.31 d and 3.54 d, respectively. The strain AVPP64 fixed 140 μg mL⁻¹ of N and solubilized 103 μg mL⁻¹ of P during the presence of 4-NA. In addition, strain AVPP64 produced significant amounts of plant growth-promoting metabolites like indole 3-acetic acid, siderophores, exo-polysaccharides and ammonia. In the presence of 4-NA and various pesticides, strain AVPP64 greatly increased the growth and biomass of Vigna radiata and Crotalaria juncea plants. These results revealed that Bacillus sp. strain AVPP64 can be used as an inoculum for bioremediation of 4-NA contaminated soil and sustainable crop production even when pesticides are present.

Bensulfuron-methyl (BSM) residues in soil threaten the rotation of BSM-sensitive crops. Microbial biofilms formed on crop roots could improve the ability of microbes to survive and protect crop roots. However, the research on biofilms with the purpose of mitigating or even eliminating BSM damage to sensitive crops is very limited. In this study, one BSM-degrading bacterium, Hansschlegelia zhihuaiae S113, colonized maize roots by forming a biofilm. Root exudates were associated with increased BSM degradation efficiency with strain S113 in rhizosphere soil relative to bulk soil, so the interactions among BSM degradation, root exudates, and biofilms may provide a new approach for the BSM-contaminated soil bioremediation. Root exudates and their constituent organic acids, including fumaric acid, tartaric acid, and l-malic acid, enhanced biofilm formation with 13.0–22.2% increases, owing to the regulation of genes encoding proteins responsible for cell motility/chemotaxis (fla/che cluster) and materials metabolism, thus promoting S113 population increases. Additionally, root exudates were also able to induce exopolysaccharide production to promote mature biofilm formation. Complete BSM degradation and healthy maize growth were found in BSM-contaminated rhizosphere soil treated with wild strain S113, compared to that treated with loss-of-function mutants ΔcheA-S113 (89.3%, without biofilm formation ability) and ΔsulE-S113 (22.1%, without degradation ability) or sterile water (10.7%, control). Furthermore, the biofilm mediated by organic acids, such as l-malic acid, exhibited a more favorable effect on BSM degradation and maize growth. These results showed that root exudates and their components (such as organic acids) can induce the biosynthesis of the biofilm to promote BSM degradation, emphasizing the contribution of root biofilm in reducing BSM damage to maize.

A native strain of Bacillus paramycoides isolated from the leachate of coal mine overburden rocks was investigated for its potential to produce selenium nanoparticles (SeNPs) by biogenic reduction of selenite, one of the most toxic forms of selenium. 16S rDNA sequencing was used to identify the bacterial strain (SP3). The SeNPs were characterized using spectroscopic (UV–Vis absorbance, dynamic light scattering, X-ray diffraction, and Raman), surface charge measurement (zeta potential), and ultramicroscopic (FESEM, EDX, FETEM) analyses. SP3 exhibited extremely high selenite tolerance (1000 mM) and reduced 10 mM selenite under 72 h to produce spherical monodisperse SeNPs with an average size of 149.1 ± 29 nm. FTIR analyses indicated exopolysaccharides coating the surface of SeNPs, which imparted a charge of −29.9 mV (zeta potential). The XRD and Raman spectra revealed the SeNPs to be amorphous. Furthermore, biochemical assays and microscopic studies suggest that selenite was reduced by membrane reductases. This study reports, for the first time, the reduction of selenite and biosynthesis of SeNPs by B. paramycoides, a recently discovered bacterium. The results suggest that B. paramycoides SP3 could be exploited for eco-friendly removal of selenite from contaminated sites with the concomitant biosynthesis of SeNPs.

In this study, the interactions between microplastics, chosen among the most widely used in industry such as polypropylene (PP) and high-density polyethylene (HDPE), and a model freshwater microalgae, Chlamydomas reinhardtii, were investigated. It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days. In parallel, a similar colonization was observed for the two polymers. However, after 20 days of contact, in the case of PP only, hetero-aggregates constituted of microalgae, microplastics and exopolysaccharides were formed. An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2. Such hetero-aggregates appear as an important pathway for the vertical transport of PP microplastics from the water surface to sediment. Moreover, after more than 70 days of contact with microplastics, the microalgae genes involved in the sugar biosynthesis pathways were strongly over-expressed compared to control conditions. The levels of over-expression were higher in the case of HDPE than in PP condition. This work presents the first evidence that depending on their chemical nature, microplastics will follow different fates in the environment.

Microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on microbial community dynamics. An experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃), was monitored for 15days and compared to control microcosm (only oil-polluted seawater, SW+OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out during all experimentation period. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil degradation was monitored by GC–MS analysis. Bioemulsificant addition stimulated an increase of the total bacterial abundance, change in the community structure and activity. The bioemulsificant also increased of 5 times the oil biodegradation rate. The data obtained from microcosm experiment indicated that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.

PAH-degrading bacteria, including Novosphingobium sp. PCY, Microbacterium sp. BPW, Ralstonia sp. BPH, Alcaligenes sp. SSK1B, and Achromobacter sp. SSK4, were isolated from mangrove sediments. These isolates degraded 50–76% of 100mg/l phenanthrene within 2weeks. Strains PCY and BPW also degraded pyrene at 98% and 71%, respectively. Furthermore, all of them probably produced biosurfactants in the presence of hydrocarbons. Interestingly, PCY has a versatility to degrade various PAHs. Molecular techniques and plasmid curing remarkably revealed the presence of the alpha subunit of pyrene dioxygenase gene (nidA), involving in its pyrene/phenanthrene degrading ability, located on megaplasmid of PCY which has never before been reported in sphingomonads. Moreover, genes encoding ferredoxin, reductase, extradiol dioxygenase (bphA3A4C) and exopolysaccharide biosynthetase, which may be involved in PAH degradation and biosurfactant production, were also found in PCY. Therefore, we conclude that these isolates, especially PCY, can be the candidates for use as inoculums in the bioremediation.

A better understanding of the effect of anthropogenic pollution on the formation of toxic Microcystis blooms is particularly important in regions with large urban centres where rivers, lakes, and estuaries receive large quantities of contaminated domestic and industrial wastes. The response of the bloom-forming cyanobacteria Microcystis aeruginosa CALU 972 and CALU 973 from Russian Karelia to pollution was investigated. The contaminants caused compensatory-adaptive changes that led to the retention of cell viability in the cyanobacterial cells. The adaptation to metals and 1,2,4-triazole was realised due to photosystem changes and the enhanced production of organic compounds, such as proteins and exopolysaccharides. Nutrients caused a significant increase in biomass production by M. aeruginosa. The exposure of M. aeruginosa to nutrients and zinc stimulated growth and contributed to enhanced microcystin concentrations. Variants of microcystins responded differently to pollution. Contaminants had pronounced effects on microcystin RR levels but less effects on microcystin LR levels. Heavy metals, 1,2,4-triazole and nitrogen influenced microcystin concentrations by affecting both the growth of Microcystis and hepatotoxin release into the environment.

The membrane fouling is the main bottleneck hindering the wide applications of anammox-membrane bioreactor (MBR). In this study, surface-coating hydrophilic modification of the membrane using polyvinyl alcohol was applied in a granular anammox-MBR. Stable anammox performance of >77% total nitrogen removal efficiency was achieved in both original and modified MBRs, along with decreasing anammox granule size. The modified membrane exhibited superior flux performance, and the membrane foulants were reduced in the MBR operation. Specifically, the foulant formation rate (f) was 0.46 g·m⁻²·d⁻¹ for the modified membrane with 100-μm coating thickness (M₁₀₀) compared with 0.75 g·m⁻²·d⁻¹ for the original membrane (M₀). However, the fouling cycle of the modified membrane with 250-μm coating thickness (M₂₅₀) was greatly shortened (5 days compared with 19 days for M₀) and f increased to 1.25 g·m⁻²·d⁻¹. Specially, the excess adhesion of exopolysaccharides and humic substances to the hydrophilic modified membrane changed the fouling layer structure and filtration resistance distribution, ultimately causing higher filtration resistance when coating thickness increased. Notably, the flux decline contribution of the concentration polarization was only 33.3% for M₀, while it was 71.3% for M₂₅₀. Finally, it was revealed that using a modified membrane increased the biological secretion rate of polysaccharide but decreased the protein bio-production rate, leading to a high PS (polysaccharide)/PN (protein) ratio in the MBR. The fouling mechanism of the hydrophilic modified membrane applied in anammox-MBR was proposed, and we highlight that the degree of hydrophilic modification is crucial to mitigating membrane fouling.

Large quantities of waste biomass are generated annually worldwide by many industries and are vastly underutilized. However, these wastes contain sugars and other dissolved organic matter and therefore can be exploited to produce microbial biopolymers. In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol. Interestingly, both yield and monosaccharide composition of EPS were affected by the carbon source. Glucose, mannose, galactose, and rhamnose were the predominant monomers, but their relative molar ratio was different. Similarly, the average molecular weight of the synthesized EPS was affected, ranging from 54.5 to 4480 kDa. The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa. Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.