The attention was focused on the diameter increment of beech timber forest growing on the partial plots with various stand density. The individual parts of the originally homogenous stand, were subjected to regeneration cutting of various intensity resulting in several partial plots. On the different partial plots we found different proportions of trees belonging to the individual diameter classes. We also found that the differences between the individual plots were statistically very significant. Beech is a tree species which is capable to continue creating considerable light increments even long after the stand opening

The study aims at assessing response patterns to chronic O3 exposure in adult forest trees, examining physiological and structural responses at the organ and whole-tree level for consistency by means of biochemical and ecophysiological analyses. Through comparison between the two O3 regimes, conclusions can be drawn about processes, which are at risk or already affected by ozone under the unchanged, prevailing stand conditions. Responses are related to the O3 influx into leaves as a measure of the physiologically effective O3 dose and cross-compared with the AOT 40 concept which is being validated on the two O3 fluxes and AOT40-related responses in biochemical, ecophysiological and structural tree parameters

Ozone (O₃) is a phytotoxic air pollutant, the adverse effects of which on growth and photosynthesis are modified by other environmental factors. In this study, we examined the combined effects of O₃, elevated CO₂, and soil nitrogen supply on Siebold's beech seedlings. Seedlings were grown under combinations of two levels of O₃ (low and two times ambient O₃ concentration), two levels of CO₂ (ambient and 700 ppm), and three levels of soil nitrogen supply (0, 50, and 100 kg N ha⁻¹ year⁻¹) during two growing seasons (2019 and 2020), with leaf photosynthetic traits being determined during the second season. We found that elevated CO₂ ameliorated O₃-induced reductions in photosynthetic activity, whereas the negative effects of O₃ on photosynthetic traits were enhanced by soil nitrogen supply. We observed three-factor interactions in photosynthetic traits, with the ameliorative effects of elevated CO₂ on O₃-induced reductions in the maximum rate of carboxylation being more pronounced under high than under low soil nitrogen conditions in July. In contrast, elevated CO₂-induced amelioration of the effects of O₃ on stomatal function-related traits was more pronounced under low soil nitrogen conditions. Although we observed several two- or three-factor interactions of gas and soil treatments with respect to leaf photosynthetic traits, the shoot to root dry mass (S/R) ratio was the only parameter for which a significant interaction was detected among seedling growth parameters. O₃ caused a significant increase in S/R under ambient CO₂ conditions, whereas no similar effects were observed under elevated CO₂ conditions. Collectively, our findings reveal the complex interactive effects of elevated CO₂ and soil nitrogen supply on the detrimental effects of O₃ on leaf photosynthetic traits, and highlight the importance of taking into consideration differences between the responses of CO₂ uptake and growth to these three environmental factors.

Polycyclic aromatic hydrocarbons (PAHs) are hazardous organic compounds with mutagenic, genotoxic and carcinogenic properties. Although PAHs in soil can cause toxicity to microorganisms, the microbial community is able to degrade these compounds. For this reason, it is important to study acute and short-term effects of PAH contamination on soil microbial community, also to shed light on its possible exploitation in soil restoration.The effects of acute PAH contamination on the structure and metabolic activity of microbial communities in three forest (beech, holm oak, black pine) soils were studied. The soils were spiked with phenanthrene, pyrene or benzo[a]pyrene and incubated in experimental mesocosms, under controlled conditions. Enzymatic activities (laccase, total peroxidase and hydrolase), as well as microbial biomass and community structure (through phospholipid fatty acid and ergosterol analyses), were evaluated in the three soil systems 4 days after contamination and compared to no-spiked soils. In soil under holm oak, there was a stimulation of Gram+ bacteria after contamination with all the 3 PAHs, whereas in soil under pine, pyrene and phenanthrene additions mainly stimulated fungi and actinomycetes.

We set up a free-air ozone (O3) exposure system for determining the photosynthetic responses of Siebold's beech (Fagus crenata) and oak (Quercus mongolica var. crispula) to O3 under field conditions. Ten-year-old saplings of beech and oak were exposed to an elevated O3 concentration (60 nmol mol−1) during daytime from 6 August to 11 November 2011. Ozone significantly reduced the net photosynthetic rate in leaves of both species in October, by 46% for beech and 15% for oak. In beech there were significant decreases in maximum rate of carboxylation, maximum rate of electron transport in photosynthesis, nitrogen content and photosynthetic nitrogen use efficiency, but not in oak. Stomatal limitation of photosynthesis was unaffected by O3. We therefore concluded photosynthesis in beech is more sensitive to O3 than that in oak, and the O3-induced reduction of photosynthetic activity in beech was due not to stomatal closure, but to biochemical limitation.

The hypothesis was tested that O₃-induced changes in leaf-level photosynthetic parameters have the capacity of limiting the seasonal photosynthetic carbon gain of adult beech trees. To this end, canopy-level photosynthetic carbon gain and respiratory carbon loss were assessed in European beech (Fagus sylvatica) by using a physiologically based model, integrating environmental and photosynthetic parameters. The latter were derived from leaves at various canopy positions under the ambient O₃ regime, as prevailing at the forest site (control), or under an experimental twice-ambient O₃ regime (elevated O₃), as released through a free-air canopy O₃ fumigation system. Gross carbon gain at the canopy-level declined by 1.7%, while respiratory carbon loss increased by 4.6% under elevated O₃. As this outcome only partly accounts for the decline in stem growth, O₃-induced changes in allocation are referred to and discussed as crucial in quantitatively linking carbon gain with stem growth.

The effects of experimentally elevated O3 on soil respiration rates, standing fine-root biomass, fine-root production and δ13C signature of newly produced fine roots were investigated in an adult European beech/Norway spruce forest in Germany during two subsequent years with contrasting rainfall patterns. During humid 2002, soil respiration rate was enhanced under elevated O3 under beech and spruce, and was related to O3-stimulated fine-root production only in beech. During dry 2003, the stimulating effect of O3 on soil respiration rate vanished under spruce, which was correlated with decreased fine-root production in spruce under drought, irrespective of the O3 regime. δ13C signature of newly formed fine-roots was consistent with the differing gs of beech and spruce, and indicated stomatal limitation by O3 in beech and by drought in spruce. Our study showed that drought can override the stimulating O3 effects on fine-root dynamics and soil respiration in mature beech and spruce forests. Drought has the capacity to override the stimulating ozone effect on soil respiration in adult European beech/Norway spruce forest.

Effects of elevated N deposition on forest aboveground biomass were evaluated using long-term data from N addition experiments and from forest observation plots in Switzerland. N addition experiments with saplings were established both on calcareous and on acidic soils, in 3 plots with Fagus sylvatica and in 4 plots with Picea abies. The treatments were conducted during 15 years and consisted of additions of dry NH4NO3 at rates of 0, 10, 20, 40, 80, and 160 kg N ha-1 yr-1. The same tree species were observed in permanent forest observation plots covering the time span between 1984 and 2007, at modeled N deposition rates of 12-46 kg N ha-1 yr-1. Experimental N addition resulted in either no change or in a decreased shoot growth and in a reduced phosphorus concentration in the foliage in all experimental plots. In the forest, a decrease of foliar P concentration was observed between 1984 and 2007, resulting in insufficient concentrations in 71% and 67% of the Fagus and Picea plots, respectively, and in an increasing N:P ratio in Fagus. Stem increment decreased during the observation period even if corrected for age. Forest observations suggest an increasing P limitation in Swiss forests especially in Fagus which is accompanied by a growth decrease whereas the N addition experiments support the hypothesis that elevated N deposition is an important cause for this development.

Stomatal O3 fluxes to a mixed beech/spruce stand (Fagus sylvatica/Picea abies) in Central Europe were determined using two different approaches. The sap flow technique yielded the tree-level transpiration, whereas the eddy covariance method provided the stand-level evapotranspiration. Both data were then converted into stomatal ozone fluxes, exemplifying this novel concept for July 2007. Sap flow-based stomatal O3 flux was 33% of the total O3 flux, whereas derivation from evapotranspiration rates in combination with the Penman-Monteith algorithm amounted to 47%. In addition to this proportional difference, the sap flow-based assessment yielded lower levels of stomatal O3 flux and reflected stomatal regulation rather than O3 exposure, paralleling the daily courses of canopy conductance for water vapor and eddy covariance-based total stand-level O3 flux. The demonstrated combination of sap flow and eddy covariance approaches supports the development of O3 risk assessment in forests from O3 exposure towards flux-based concepts.