Little is known about the fate of oil spills in rivers. Hyporheic flows of water through river sediments exchange surface and groundwater and create upwelling and downwelling zones that are important for fish spawning and embryo development. Risk assessments of oil spills to rivers do not consider the potential for hyporheic flows to carry oil droplets into sediments and the potential for prolonged exposure of fish to trapped oil. This project assessed whether oil droplets in water flowing through gravel will be trapped and whether hydrocarbons partitioning from trapped oil droplets are bioavailable to fish. Columns packed with gravel were injected with oil-in-water dispersions prepared with light crude, medium crude, diluted bitumens, and heavy fuel oil to generate a series of oil droplet loadings. The concentrations of oil trapped in the gravel increased with oil loading and viscosity. When the columns were perfused with clean water, oil concentrations in column effluents decreased to the detection limit within the first week of water flow, with sporadically higher concentrations associated with oil droplet release. Despite the low concentrations of hydrocarbons measured in column effluent, hydrocarbons were bioavailable to juvenile rainbow trout (Oncorhynchus mykiss) for more than three weeks of water flow, as indicated by strong induction of liver ethoxyresorufin-o-deethylase activity. These findings indicate that ecological risk assessments and spill response should identify and protect areas in rivers sensitive to contaminant trapping.

Many heavy metals in sediments and water have potential adverse effects on aquatic organisms such as Corbicula fluminea (O.F. Müller, 1774), a bivalve species frequently used as a biomonitor for metal pollution. Studies over the past decades examining the heavy metal uptake by C. fluminea, very few has investigated the effect of hydrodynamic conditions on accumulation of heavy metal by C. fluminea. Therefore, in this study, to investigate the mechanism of intracellular and extracellular accumulation of metal, individuals of C. fluminea were exposed to cadmium (Cd)-treated water under three different hydrodynamic conditions. These included exposures in two set ups: three rates of rotation (500, 350, 200 r/min) in beakers for 10 days, and then exposure to Cd-treated sediment under two naturally turbulent water conditions (14 cm/s and 3.2 cm/s) in experimental flumes for 23 days. Hydrodynamic force increased the burrowing rate but decreased the activity of C. fluminea. After 10 days of exposure, the extracellular concentrations of Cd in the tissues of C. fluminea in the sand group were significantly higher than that in the gravel groups. The intracellular and extracellular concentrations of Cd in the tissues of C. fluminea dramatically increased in the Cd-treated sediment test. Moreover, the concentration of the extracellular Cd adsorbed on the tissues of C. fluminea in the 14 cm/s and 3.2 cm/s groups was significantly higher than that in the control group, whereas the effect of hydrodynamic force on absorption of Cd by C. fluminea was not obvious. These results suggest that hydrodynamic condition plays an important role in extracellular accumulation of Cd by C. fluminea. In future study, when using C. fluminea to assess Cd pollution in aquatic environment, extracellular Cd adsorbed on the tissue should be removed to avoid the influence of hydrodynamics.

In this pilot-scale constructed wetland (CW) study for treating groundwater contaminated with benzene, MTBE, and ammonia-N, the performance of two types of CWs (a wetland with gravel matrix and a plant root mat) was investigated. Hypothesized stimulative effects of filter material additives (charcoal, iron(III)) on pollutant removal were also tested. Increased contaminant loss was found during summer; the best treatment performance was achieved by the plant root mat. Concentration decrease in the planted gravel filter/plant root mat, respectively, amounted to 81/99% for benzene, 17/82% for MTBE, and 54/41% for ammonia-N at calculated inflow loads of 525/603 mg/m²/d, 97/112 mg/m²/d, and 1167/1342 mg/m²/d for benzene, MTBE, and ammonia-N. Filter additives did not improve contaminant depletion, although sorption processes were observed and elevated iron(II) formation indicated iron reduction. Bacterial and stable isotope analysis provided evidence for microbial benzene degradation in the CW, emphasizing the promising potential of this treatment technique.

The behaviors of typical veterinary antibiotics (oxytetracycline, ciprofloxacin and sulfamethazine) and 75 types of corresponding antibiotic resistant genes (ARGs) in four substrate systems (zeolite, gravel, red brick, and oyster shell) were investigated in this study. The results indicated that during treating synthetic livestock wastewater with individual antibiotic influent concentration of 100 μg/L, the effluent contained oxytetracycline and ciprofloxacin concentrations of 0.7–1.5 μg/L and 1.0–1.9 μg/L, respectively, in the zeolite and red brick systems, which were significantly lower than those of the other substrate systems (4.6–14.5 μg/L). Statistical correlation analyses indicated that the difference regarding oxytetracycline and ciprofloxacin removal among the four substrates was determined by their adsorption capacity which was controlled by the chemisorption mechanism. The average removal efficiency of sulfamethazine in the gravel system (48%) was higher than that of the other substrate systems (34–45%), and biodegradation may alter the sulfamethazine performance because of its co-metabolism process. Although tetG, floR, sul1, and qacEΔ1 were the dominant ARGs in all substrate systems (8.74 × 10⁻²-6.34 × 10⁻¹), there was difference in the total ARG enrichment levels among the four substrates. Oyster shell exhibited the lowest total relative abundance (1.56 × 10⁰) compared to that of the other substrates (1.82 × 10⁰–2.27 × 10⁰), and the ARG total relative abundance exhibited significant negative and positive correlations with the substrate pH and system bacterial diversity (P < 0.05), respectively. In summary, this study indicated that due to the difference of adsorption capacity and residual abundant nutrient in wastewater, the wetland substrate selection can affect the removal efficiency of veterinary antibiotics, and antibiotics may not be the determining factor of ARG enrichment in the substrate system.

The performance of the radon (²²²Rn)-deficit technique has been evaluated at a site in which a complex DNAPL mixture (mostly hexachlorocyclohexanes and chlorobenzenes) has contaminated all four layers (from top to bottom: anthropic backfill, silt, gravel and marl) of the soil profile. Soil gas samples were collected at two depths (0.8 m and 1.7 m) in seven field campaigns and a total of 186 ²²²Rn measurements were performed with a pulse ionization detector. A statistical assessment of the influence of field parameters on the results revealed that sampling depth and atmospheric pressure did not significantly affect the measurements, while the location of the sampling point and ground-level atmospheric temperature did. In order to remove the bias introduced by varying field temperatures and hence to be able to jointly interpret ²²²Rn measurements from different campaigns, ²²²Rn concentrations were rescaled by dividing each individual datum by the mean ²²²Rn concentration of its corresponding field campaign. Rescaled ²²²Rn maps showed a high spatial correlation between ²²²Rn minima and maximum contaminant concentrations in the top two layers of the soil profile, successfully delineating the surface trace of DNAPL accumulation in the anthropic backfill and silt layers. However, no correlation could be established between ²²²Rn concentrations in superficial soil gas and contaminant concentration in the deeper two layers of the soil profile. These results indicate that the ²²²Rn-deficit technique is unable to describe the vertical variation of contamination processes with depth but can be an effective tool for the preliminary characterization of sites in which the distance between the inlet point of the sampling probe and the contaminant accumulation falls within the effective diffusion length of ²²²Rn in the affected soil profile.

Evidence from the past shows that pesticide use in populated areas may impact groundwater quality. The approval of herbicides such as diflufenican and glyphosate for use on paved and unpaved gravel surfaces in the European Union is based on their behaviour and fate in agricultural soils. However, this might be very different from their fate in gravel surfaces. We therefore conducted an outdoor study with 21 small lysimeters containing different gravel types and a sandy arable topsoil as control. The lysimeters were sprayed with a commercial product for gardening, containing diflufenican and glyphosate. The concentrations of the herbicides and their relevant degradation products in the outlet was followed for 19 months. Diflufenican, glyphosate and AMPA did not leach from any of the lysimeters. However, one diflufenican degradation product (AE-0) leached from two of the gravel types for more than a year and a second degradation product (AE-B) leached from all gravels for up to one year. Concentrations in the leachate peaked at 0.5–3 μg/L, with highest concentrations over the longest periods observed with rock chippings on top of the gravel. We conclude, that the different properties of gravel compared to those of agricultural soils may lead to very different herbicide leaching patterns but also that the leaching depends highly on the type of gravel and type of herbicide.

As phytoextraction implementation may be limited by metal toxicity and leaching, we investigated the idea of in situ metal immobilization in bulk soil, while increasing metal bioavailability in the rhizosphere. Salix smithiana was grown in a pot experiment on two Cd/Zn polluted soils. Treatments with or without willows included: no additives; gravel sludge + red mud kg⁻¹; acidification with S to pH 3.5; and metal immobilization combined with soil acidification. Salix smithiana removed up to 0.78 ± 0.06% total Cd and 0.34% (±0.02) total Zn from the non-treated soils. The phytoextraction efficiency in the S treatments was enhanced by up to ∼50% in response to metal solubility that was magnified by reductive co-dissolution from Mn (IV) and Fe(III) (oxy)hydroxides during microbial S oxidation in the willow rhizosphere. The proposed technique proved to enhance phytoextraction efficiency while controlling the risk of metal leaching from the root zone and phytotoxicity.

Constructed wetlands (CWs) inoculated with exogenous microbes have great potential for removing pollutants in adverse environments. The rapid loss of functional bacteria and the high cost of repeated additions of inoculum, however, limit the practical application of this technology. In this study, C–F2 immobilized bacteria (i.e., immobilized salt-tolerant bacterium Alishewanella sp. F2 incorporated with a carbon source) were developed and utilized in CWs for solving the above problems. A 60-day experiment demonstrated that bioaugmented CWs (Bio-CWs) with the addition of C–F2 immobilized bacteria into the bottom gravel layer of CW microcosms (B-CF2 treatment) exhibited high nitrogen removal efficiency under a saline condition (electrical conductivity of 15 mS/cm). We measured mean nitrate nitrogen (NO₃⁻-N) and total nitrogen (TN) removal percentages of 97.8% and 88.1%, respectively, which were significantly (p < 0.05) higher than those in Bio-CWs with microbial inoculum (MI-F2 treatment, 63.5% and 78.2%) and unbioaugmented CWs (CK, 48.7% and 67.2%). The TN content of the entire plant was significantly (p < 0.05) increased in B-CF2 (636.06 mg/microcosm) compared with CK (372.06 mg/microcosm). The relative abundances of the genera Alishewanella (i.e., the exogenous bacterium, 5.5%), Clostridium-XlVa (8.8%) and Bacteroides (21.1%) in B-CF2 were significantly (p < 0.05) higher than in MI-F2 and CK, which improved the denitrification capacity of CWs. Overall, a high denitrification efficiency and durability were achieved in the newly developed Bio-CWs (i.e., B-CF2 treatment) with immobilized bacteria under saline conditions, which provides an alternative technology for the rapid removal of nitrogen from saline wastewater.

The Yangtze estuary is considered as an important eco-region of China, but it has been heavily disturbed by reclamation projects. We evaluated the effect of freshwater inflow on the self-restoration of macrobenthic diversity in 3-year-old vegetated, gravel, and mudflat habitats of reclaimed seaward intertidal wetlands. There was a significant self-restoration of species richness and diversity in vegetated and gravel habitats with no inflow of freshwater, but there were no significant changes in species diversity in three habitats subjected to freshwater inflow. Significant changes in community structures of macrobenthos occurred in vegetated and gravel habitats from pre- to post-restoration. Analysis of benthic health showed that gravel habitats were under moderate to high levels of disturbance in pre-restoration period. In contrast, there tended to be no evidence of disturbance in vegetated and mudflat habitats during two investigations. Restoration of macrobenthos is best achieved in vegetated habitats with on inflow of freshwater.

The molluscan assemblages in a sediment core from the north-eastern Adriatic show significant compositional changes over the past 10,000yrs related to (1) natural deepening driven by the post-glacial sea-level rise, (2) increasing abundance of skeletal sand and gravel, and (3) anthropogenic impacts. The transgressive phase (10,000–6000 BP) is characterized by strongly time-averaged communities dominated by infaunal bivalves. During the early highstand (6000–4000 BP), the abundance of epifaunal filter feeders and grazers increases, and gastropods become more important. Epifaunal dominance culminates during the late highstand (4000–2000 BP) with the development of extensive shell beds formed by large-sized Arca noae and Ostrea sp. bivalves. This community persists until the early 20th century, when it falls victim to multiple anthropogenic impacts, mainly bottom trawling, and is substituted by an infauna-dominated community indicative of instability, disturbance and organic enrichment. The re-establishment of this unique shell-bed ecosystem can be a goal for restoration efforts.