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Membrane enzymes as possible biosenzor for lead monitoring in water
1997
Nikezic, G. (Institut za nuklearne nauke Vinca, Beograd (Yugoslavia). Laboratorija za molekularnu biologiju i endokrinologiju) | Horvat, A. | Todorovic, S. | Vasic, V. | Vujisic, Lj.
In our earlier work, we have shown that enzymes from rat brain synaptosomal membranes, Na(+)/K(+)-ATPase and Mg(2+)-ATPase, are promising biological components of a biosensor for lead detection. In this work, we represent our results of investigation with the same enzymes as biological components for the biosensor in presence of Hg(2+) ions in water. It was established that IC50 for Na(+)/K(+)-ATPase and Mg(2+)-ATPase is 6.9 and 5.5 x 10E-6 M, and the percentages of inhibition are 96% and 77% respectively. We concluded that these enzymes could be the base for developing biosensors for the presence of Hg(2+) ion in water. Since these enzymes maintain a stable activity for a longer period of time, they could be appropriate as components of biosensors for monitoring water quality.
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1997
Bervoets, L. | Blust, R. | Wit, M. de | Verheyen, R. (Department of Biology, University of Antwerp (UIA), Universiteitsplein I, 2610, Wilrijk (Belgium))
Decomposition of pine-litter organic matter and chemical properties of upper soil layers: transect studies
1997
Breymeyer, A. | Degorski, M. | Reed, D. (Institute of Geography and Spatial Organization PAS, Twarda 51/55, 00-818 Warsaw (Poland))
Cu accumulation in the earthworm Dendrobaena veneta in a heavy metal (Cu, Pb, Zn) contaminated site compared to Cu accumulation in laboratory experiments
1997
Marinussen, M.P.J.C. | Zee, S.E.A.T.M. van der | Haan, F.A.M. de (Wageningen Agricultural University, Department of Soil Science and Plant Nutrition, PO Box 8005, 6700 EC Wageningen (Netherlands))
Spatial and temporal distribution of metals in soils in Warsaw, Poland
1997
Pichtel, J. | Sawyerr, H.T. | Czarnowska, K. (Ball State University, Natural Resources and Environmental Management, Muncie, Indiana 47306-0495 (USA))
Bioaccumulation and physiological effects of excess lead in a roadside pioneer species Sonchus oleraceus L
1997
Xiong ZhiTing (Department of Environmental Science, Wuhan University, Wuhan, Hubei 430072 (China))
Speciation of lead in contaminated soils
1997
Sauve, S. | McBride, M.B. | Hendershot, W.H. (Cornell University, SCAS Department, Bradfield Hall 908, Ithaca, New York, 14853 (USA))
Mobility of Zn, Cd and Pb in soils as affected by poultry litter extract-II. Redistribution among soil fractions
1997
Li ZhenBin | Shuman, L.M. (Department of Crop and Soil Science, Georgia Experiment Station, University of Georgia, Griffin, GA 30223-1797 (USA))
Mobility of Zn, Cd and Pb in soils as affected by poultry litter extract-I. Leaching in soil columns
1997
Li ZhenBin | Shuman, L.M. (Department of Crop and Soil Science, Georgia Experiment Station, University of Georgia, Griffin, GA 30223-1797 (USA))
Metal extractability and availability in a soil after heavy application of either nickel or lead in different forms
1997
NOGALES, R. | GALLARDO-LARA, F. | BENITEZ, E. | Soto, J. | HERVAS, D. | Polo, A.
A greenhouse experiment using soil was conducted to investigate the effects of the addition of different forms of either nickel or lead, together with an acidifying agent, on the distribution of Ni, Pb, Zn, Cu and Mn in wheat plants, and on the post-harvest extractability of these elements in the soil. Two treatments consisting of soil alone or soil mixed with sewage sludge at a rate of 200 Mg ha⁻¹ were used as controls. Nickel (400 mg kg⁻¹) or lead (1600 mg kg⁻¹) was added to the soil as an inorganic salt or mixed previously with sewage sludge. Six further treatments including an acidifying agent (wastewater from olive oil processing: alpechin) were also prepared. Wheat (Triticum aestivum L. var. Mesa) plants were harvested 75 d after germination. Dry matter yield of wheat was increased by the addition of sewage sludge. No reductions in yield were observed after the addition of nickel or lead. Nickel concentration and uptake by wheat, and extractability from soil, were higher when the sewage sludge enriched in nickel was added to soil. This effect was enhanced when the acidifying agent was also added. In contrast, lead availability was higher after the addition of inorganic Pb to soil. The addition of both forms of Ni enhanced Zn, Cu and Mn uptake by the plant, whereas the addition of lead increased Zn and Cu. After harvesting, increases in extractable Zn and Cu in the soil were observed only in treatments with sewage sludge, and not after the addition of Ni or Pb, or after the addition of the acidifying agent. Decreasing the pH of the soil with the acidifying agent tended to increase Mn uptake by wheat, and Mn extractability from the soil after harvesting.
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