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Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress
2015
Xue, Liang | Ren, Huadong | Li, Sheng | Gao, Ming | Shi, Shengqing | Chang, Ermei | Wei, Yuan | Yao, Xiaohua | Jiang, Zeping | Liu, Jianfeng
To explore the molecular basis of Sb tolerance mechanism in plant, a comparative proteomic analysis of both roots and leaves in Miscanthus sinensis has been conducted in combination with physiological and biochemical analyses. M. sinensis seedlings were exposed to different doses of Sb, and both roots and leaves were collected after 3 days of treatment. Two-dimensional gel electrophoresis (2-DE) and image analyses found that 29 protein spots showed 1.5-fold change in abundance in leaves and 19 spots in roots, of which 31 were identified by MALDI-TOF-MS and MALDI-TOF-TOF-MS. Proteins involved in antioxidant defense and stress response generally increased their expression all over the Sb treatments. In addition, proteins relative to transcription, signal transduction, energy metabolism and cell division and cell structure showed a variable expression pattern over Sb concentrations. Overall these findings provide new insights into the probable survival mechanisms by which M. sinensis could be adapting to Sb phytotoxicity.
显示更多 [+] 显示较少 [-]Non-enhanced phytoextraction of cadmium, zinc, and lead by high-yielding crops
2017
Mayerová, Markéta | Petrová, Šárka | Madaras, Mikuláš | Lipavský, Jan | Šimon, Tomáš | Vaněk, Tomáš
Heavy metal soil contamination from mining and smelting has been reported in several regions around the world, and phytoextraction, using plants to accumulate risk elements in aboveground harvestable organs, is a useful method of substantially reducing this contamination. In our 3-year experiment, we tested the hypothesis that phytoextraction can be successful in local soil conditions without external fertilizer input. The phytoextraction efficiency of 15 high-yielding crop species was assessed in a field experiment performed at the Litavka River alluvium in the Příbram region of Czechia. This area is heavily polluted by Cd, Zn, and Pb from smelter installations which also polluted the river water and flood sediments. Heavy metal concentrations were analyzed in the herbaceous plants’ aboveground and belowground biomass and in woody plants’ leaves and branches. The highest Cd and Zn mean concentrations in the aboveground biomass were recorded in Salix x fragilis L. (10.14 and 343 mg kg⁻¹ in twigs and 16.74 and 1188 mg kg⁻¹ in leaves, respectively). The heavy metal content in woody plants was significantly higher in leaves than in twigs. In addition, Malva verticillata L. had the highest Cd, Pb, and Zn concentrations in herbaceous species (6.26, 12.44, and 207 mg kg⁻¹, respectively). The calculated heavy metal removal capacities in this study proved high phytoextraction efficiency in woody species; especially for Salix × fragilis L. In other tested plants, Sorghum bicolor L., Helianthus tuberosus L., Miscanthus sinensis Andersson, and Phalaris arundinacea L. species are also recommended for phytoextraction.
显示更多 [+] 显示较少 [-]Revegetation of a barren rare earth mine using native plant species in reciprocal plantation: effect of phytoremediation on soil microbiological communities
2020
Zhang, Lin | Liu, Wen | Liu, Shenghong | Zhang, Peng | Ye, Chanjuan | Liang, Hong
Over-exploration of rare earth elements causes soil desertification and environmental degradation. However, the restoration of rare earth mine tailings requires the recovery of both vegetation and soil microbiota. Accordingly, the present study aimed to compare the efficacy of restoring mine tailings using organic compost and native plants (Miscanthus sinensis, Pinus massoniana, Bambusa textilis, or a mixture of all three). After three years, the mixed plantation harbored tenfold greater plant richness than that in the barren land. Among these, M. sinensis played a dominant role across all restored areas. The microbial communities of the soils were assessed using high-throughput 16S rDNA gene sequencing. A total of 34,870 16S rDNA gene sequences were obtained and classified into 15 bacterial phyla and 36 genera. The dominant genus across all the restored soils was Burkholderia, and the bacterial diversity of restored soils was greater than that of soils from either unrestored or natural (unexploited) areas, with the M. sinensis plantation yielding the greatest diversity. The effects of phytoremediation were mainly driven by changes in nutrient and metal contents. These results indicate that M. sinensis significantly improves phytoremediation and that mixed planting is ideal for restoring the soils of abandoned rare earth mines.
显示更多 [+] 显示较少 [-]Phytoremediation potential of Miscanthus sinensis for mercury-polluted sites and its impacts on soil microbial community
2019
Zhao, Anqi | Gao, Lingyun | Chen, Buqing | Feng, Liu
Phytoremediation potential of Miscanthus sinensis and its impacts on soil microbial community and nutrients were evaluated by pot experiment at soil mercury concentration from 1.48 to 706 mg kg⁻¹. The changes in biomass yield in dry mass, chlorophyll content, and SOD activity indicated Miscanthus sinensis was tolerant to higher levels of soil mercury exposure, and could grow even if at soil mercury up to 706 mg kg⁻¹. Mercury bioconcentration and translocation factors were close to or greater than 1 when exposed to soil mercury up to 183 mg kg⁻¹, demonstrating Miscanthus sinensis a potential phytoremediator for mercury-polluted soils. Miscanthus sinensis planting could significantly improve the diversity and abundance of soil microbial community, but might cause potential loss of soil nitrogen and phosphorus in the early and middle of its growth. In a word, the study indicated Miscanthus sinensis was a promising energy crop linking biofuel production and phytoremediation of mercury-contaminated sites.
显示更多 [+] 显示较少 [-]Ornamental plants for micropollutant removal in wetland systems
2015
Macci, Cristina | Peruzzi, Eleonora | Doni, Serena | Iannelli, Renato | Masciandaro, Grazia
The objective of this paper was to evaluate the efficiency of micropollutant removal, such as Cu, Zn, carbamazepine, and linear alkylbenzene sulfonates (LAS), through the use of a subsurface vertical flow constructed wetland system with ornamental plants. Zantedeschia aethiopica, Canna indica, Carex hirta, Miscanthus sinensis, and Phragmites australis were selected and planted in lysimeters filled up with gravel. The lysimeters were completely saturated with synthetic wastewater (N 280 mg L⁻¹, P 30 mg L⁻¹, Cu 3.6 mg L⁻¹, Zn 9 mg L⁻¹, carbamazepine 5 μg L⁻¹, linear alkylbenzene sulfonates 14 mg L⁻¹), and the leaching water was collected for analysis after 15, 30, and 60 days in winter-spring and spring-summer periods. Nutrients (N and P) and heavy metals decreased greatly due to both plant activity and adsorption. C. indica and P. australis showed the highest metal content in their tissues and also the greatest carbamazepine and LAS removal. In these plants, the adsorption/degradation processes led to particularly high oxidative stress, as evidenced by the significantly high levels of ascorbate peroxidase activity detected. Conversely, Z. aethiopica was the less efficient plant in metal and organic compound removal and was also less stressed in terms of ascorbate peroxidase activity.
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