To date there have only been a few studies that measured mercury emissions from background substrate worldwide, and only a small amount of mercury flux data, from background substrate, exists for the Western United States. Because of this, the database of mercury emissions from background units < 0.1 mg kg⁻¹ mercury) is incomplete. This study focused on the collection of in-situ mercury flux data from representative lithologic units in Nevada. Measured mercury fluxes from substrate with background mercury concentration throughout Nevada were low (mean 2.0 ± 4.1 ng m⁻² hr⁻¹), and ranged from –3.7 to 9.3 ng m⁻² hr⁻¹. The mean measured mercury flux is slightly higher than those measured from background substrate from various locations throughout the world. The mean mercury flux from in-situ mercury measurements from substrate located near altered geologic units across Nevada was 15.5 ± 24.2 ng m⁻² hr⁻¹. These mercury fluxes are higher than the values applied in published global models for naturally enriched geologic units.