细化搜索
结果 1-10 的 71
Bioaccumulation of heavy metals, metalloids, and chlorine in ectomycorrhizae from smelter-polluted area
2016
Cejpková, Jaroslava | Gryndler, Milan | Hršelová, Hana | Kotrba, Pavel | Řanda, Zdeněk | Synková, Iva | Borovička, Jan
Ectomycorrhizal (ECM) fungi contribute to the survival of host trees on metal-rich soils by reducing the transfer of toxic metals into roots. However, little is known about the ability of ECM fungi to accumulate elements in ectomycorrhizae (ECMs). Here we report Ag, As, Cd, Cl, Cu, Sb, V, and Zn contents in wild-grown Norway spruce ECMs collected in a smelter-polluted area at Lhota near Příbram, Czech Republic. The ECMs data were compared with the element concentrations determined in the corresponding non-mycorrhizal fine roots, soils, and soil extracts. Bioaccumulation factors were calculated to differentiate the element accumulation ability of ECMs inhabited by different mycobionts, which were identified by ITS rDNA sequencing. Among the target elements, the highest contents were observed for Ag, Cl, Cd, and Zn; Imleria badia ECMs showed the highest capability to accumulate these elements. ECMs of Amanita muscaria, but not of other species, accumulated V. The analysis of the proportions of I. badia and A. muscaria mycelia in ECMs by using species-specific quantitative real-time PCR revealed variable extent of the colonization of roots, with median values close to 5% (w/w). Calculated Ag, Cd, Zn and Cl concentrations in the mycelium of I. badia ECMs were 1 680, 1 510, 2 670, and 37,100 mg kg−1 dry weight, respectively, indicating substantial element accumulation capacity of hyphae of this species in ECMs. Our data strengthen the idea of an active role of ECM fungi in soil-fungal-plant interactions in polluted environments.
显示更多 [+] 显示较少 [-]Micronized copper wood preservatives: An efficiency and potential health risk assessment for copper-based nanoparticles
2015
Civardi, Chiara | Schwarze, Francis W.M.R. | Wick, Peter
Copper (Cu) is an essential biocide for wood protection, but fails to protect wood against Cu-tolerant wood-destroying fungi. Recently Cu particles (size range: 1 nm–25 μm) were introduced to the wood preservation market. The new generation of preservatives with Cu-based nanoparticles (Cu-based NPs) is reputedly more efficient against wood-destroying fungi than conventional formulations. Therefore, it has the potential to become one of the largest end uses for wood products worldwide. However, during decomposition of treated wood Cu-based NPs and/or their derivate may accumulate in the mycelium of Cu-tolerant fungi and end up in their spores that are dispersed into the environment. Inhaled Cu-loaded spores can cause harm and could become a potential risk for human health. We collected evidence and discuss the implications of the release of Cu-based NPs by wood-destroying fungi and highlight the exposure pathways and subsequent magnitude of health impact.
显示更多 [+] 显示较少 [-]Heavy metal fixation of lead-contaminated soil using Morchella mycelium
2021
Wang, Yazhou | Tan, Renhao | Zhou, Li | Lian, Jie | Wu, Xudong | He, Rong | Yang, Fan | He, Xinsheng | Zhu, Wenkun
With the exploitation of lead-zinc deposits, lead content around mining areas has seriously exceeded the recommended level. The most challenging problem is how to reduce lead contamination in soil efficiently. In this study, we developed a method to remediate lead-contaminated soil by adding Morchella mycelium. First, we compared the repair effects of mycelium and hyperaccumulator by conducting pot experiments. Then, we investigated the mechanism through which mycelium repairs lead-contaminated soil by conducting simulation experiments. Results showed that using mycelium was a more efficient way to repair soil than using hyperaccumulator. Compared with the untreated group, mycelium reduced the lead content of crops by 34.83 % and raised dry biomass by 134.05 % when lead addition was 800 mg/kg. After mycelium fixation, soil catalase, urease, cellulase, and sucrase activities were significantly enhanced, and the bioavailability of lead decreased significantly. The lead solution exposure simulation test showed that Morchella mycelium immobilized lead due to its extracellular secretions. That is, mycelium secreted metabolites and lead to form salt crystals, reducing bioavailable lead content. In addition, Morchella mycelium restoration may effectively improve soil fertility and increase crop yields. Thus, mycelium may be used successfully in alternative green repair methods for environmental heavy metal remediation.
显示更多 [+] 显示较少 [-]Changes in mycelia growth, sporulation, and virulence of Phytophthora capsici when challenged by heavy metals (Cu2+, Cr2+ and Hg2+) under acid pH stress
2018
Liu, Peiqing | Wei, Mengyao | Zhang, Jinzhu | Wang, Rongbo | Li, Benjin | Chen, Qinghe | Weng, Qiyong
Phytophthora capsici, an economically devastating oomycete pathogen, causes devastating disease epidemics on a wide range of vegetable plants and pose a grave threat to global vegetables production. Heavy metals and acid pH are newly co-occurring stresses to soil micro-organisms, but what can be expected for mycelia growth and virulence and how they injure the oomycetes (especially P. capsici) remains unknown. Here, the effects of different heavy metals (Cu²⁺, Cr²⁺, and Hg²⁺) on mycelia growth and virulence were investigated at different pHs (4.0 vs. 7.0) and the plausible molecular and physiological mechanisms were analyzed. In the present study, we compared the effective inhibition of different heavy metals (Cu²⁺, Cr²⁺, and Hg²⁺) and acid pH on a previously genome sequenced P. capsici virulent strain LT1534. Both stress factors independently affected its mycelia growth and sporulation. Next, we investigated whether ROS participated in the pH-inhibited mycelial growth, finding that the ROS scavenger, catalase (CAT), significantly inhibited the acid pH-induced ROS in mycelia. Additionally, because MAPK specially transmits different stress responsive signals in environment into cells, we employed CAT and a p38-MAPK pathway inhibitor to investigate ROS and p38-MAPK roles in heavy metal-inhibited mycelia growth at different pHs (4.0 vs. 7.0), finding that they significantly inhibited growth. Furthermore, ROS and p38-MAPK influenced the heavy metal-induced TBARS content, total antioxidant capacity (TAC), and CAT activity at different pHs, and also reduced the expression of infection-related laccases (PcLAC2) and an effector-related protein (PcNLP14). We propose that acid pH stress accelerates how heavy metals inhibit mycelium growth, sporulation, and virulence change in P. capsici, and posit that ROS and p38-MAPK function to regulate the molecular and physiological mechanisms underlying this toxicity. Although these stresses induce molecular and physiological challenges to oomycetes, much remains to be known the mechanisms dedicated to resolve these environmental stresses.
显示更多 [+] 显示较少 [-]Biodegradation of polyester polyurethane by Aspergillus tubingensis
2017
Khan, Sehroon | Nadir, Sadia | Shah, Zia Ullah | Shah, Aamer Ali | Karunarathna, Samantha C. | Xu, Jianchu | Khān, Āfsar | Munir, Shahzad | Hasan, Fariha
The xenobiotic nature and lack of degradability of polymeric materials has resulted in vast levels of environmental pollution and numerous health hazards. Different strategies have been developed and still more research is being in progress to reduce the impact of these polymeric materials. This work aimed to isolate and characterize polyester polyurethane (PU) degrading fungi from the soil of a general city waste disposal site in Islamabad, Pakistan. A novel PU degrading fungus was isolated from soil and identified as Aspergillus tubingensis on the basis of colony morphology, macro- and micro-morphology, molecular and phylogenetic analyses. The PU degrading ability of the fungus was tested in three different ways in the presence of 2% glucose: (a) on SDA agar plate, (b) in liquid MSM, and (c) after burial in soil. Our results indicated that this strain of A. tubingensis was capable of degrading PU. Using scanning electron microscopy (SEM), we were able to visually confirm that the mycelium of A. tubingensis colonized the PU material, causing surface degradation and scarring. The formation or breakage of chemical bonds during the biodegradation process of PU was confirmed using Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy. The biodegradation of PU was higher when plate culture method was employed, followed by the liquid culture method and soil burial technique. Notably, after two months in liquid medium, the PU film was totally degraded into smaller pieces. Based on a comprehensive literature search, it can be stated that this is the first report showing A. tubingensis capable of degrading PU. This work provides insight into the role of A. tubingensis towards solving the dilemma of PU wastes through biodegradation.
显示更多 [+] 显示较少 [-]Tracking the conversion of nitrogen during pyrolysis of antibiotic mycelial fermentation residues using XPS and TG-FTIR-MS technology
2016
Zhu, Xiangdong | Yang, Shijun | Wang, Liang | Liu, Yuchen | Qian, Feng | Yao, Wenqing | Zhang, Shicheng | Chen, Jianmin
Antibiotic mycelial fermentation residues (AMFRs), which are emerging solid pollutants, have been recognized as hazardous waste in China since 2008. Nitrogen (N), which is an environmental sensitivity element, is largely retained in AMFR samples derived from fermentation substrates. Pyrolysis is a promising technology for the treatment of solid waste. However, the outcomes of N element during the pyrolysis of AMFRs are still unknown. In this study, the conversion of N element during the pyrolysis of AMFRs was tracked using XPS (X-ray photoelectron spectroscopy) and online TG-FTIR-MS (Thermogravimetry-Fourier transform infrared-Mass spectrometry) technology. In the AMFR sample, organic amine-N, pyrrolic-N, protein-N, pyridinic-N, was the main N-containing species. XPS results indicated that pyrrolic-N and pyridinic-N were retained in the AMFR-derived pyrolysis char. More stable species, such as N-oxide and quaternary-N, were also produced in the char. TG-FTIR-MS results indicated that NH3 and HCN were the main gaseous species, and their contents were closely related to the contents of amine-N and protein-N, and pyrrolic-N and pyridinic-N of AMFRs, respectively. Increases in heating rate enhanced the amounts of NH3 and HCN, but had less of an effect on the degradation degree of AMFRs. N-containing organic compounds, including amine-N, nitrile-N and heterocyclic-N, were discerned from the AMFR pyrolysis process. Their release range was extended with increasing of heating rate and carbon content of AMFR sample. This work will help to take appropriate measure to reduce secondary pollution from the treatment of AMFRs.
显示更多 [+] 显示较少 [-]Phytobarriers: Plants capture particles containing potentially toxic elements originating from mine tailings in semiarid regions
2015
Sánchez-López, Ariadna S. | Carrillo González, Rogelio | González Chávez, Ma. del Carmen Ángeles | Rosas-Saito, Greta Hanako | Vangronsveld, Jaco
Retention of particles containing potentially toxic elements (PTEs) on plants that spontaneously colonize mine tailings was studied through comparison of washed and unwashed shoot samples. Zn, Pb, Cd, Cu, Ni, Co and Mn concentrations were determined in plant samples. Particles retained on leaves were examined by Scanning Electronic Microscopy and energy dispersive X-Ray analysis. Particles containing PTEs were detected on both washed and unwashed leaves. This indicates that the thorough washing procedure did not remove all the particles containing PTEs from the leaf surface, leading to an overestimation of the concentrations of PTEs in plant tissues. Particularly trichomes and fungal mycelium were retaining particles. The quantity and composition of particles varied among plant species and place of collection. It is obvious that plants growing on toxic mine tailings form a physical barrier against particle dispersion and hence limit the spread of PTEs by wind.
显示更多 [+] 显示较少 [-]The fungus Pestalotiopsis sp., isolated from Perna perna (Bivalvia:Mytilidae) cultured on marine farms in Southeastern Brazil and destined for human consumption
2020
dos Santos, Antonia Lúcia | de Medeiros, João Victor Ferreira | Grault, Carlos Eduardo | Santos, Manoel José Soares | Souza, André Luiz Almeida | de Carvalho, Raimundo Wilson
The Pestalotiopsis sp. genus comprises filamentous fungi whose species present both pathogenic and clinical-industrial importance. The cultivation and production of bivalve molluscs in regions of Rio de Janeiro is lucrative both artisanally and industrially, as the climate, geography and water quality favor the practice of this activity throughout the year at reduced costs, making the region competitive in the national market. The aim of this study was to isolate and identify filamentous fungi associated with the internal tissue anatomy of bivalve molluscs (Perna perna) from mariculture farms. Samples collected from BEMAR marine farms were dissected and transferred to 1% hypochlorite, washed in sterile distilled water and sown on Petri dishes containing potato dextrose agar (BDA). After four days, a white colony, displaying vigorous mycelium, cotton-like with abundant sporulation and black conidia masses was isolated. Observations concerning vegetative and reproductive structures were performed by microcultures stained with Amann's Lactophenol andCotton Blue. Micromorphology analyses indicated spindle and septated conidia, with two to three apical filiform appendages and a short basal pedicel. The result indicates that bivalve mollusks may be bioindicators for the presence of Pestalotiopsis sp; associated with water transport, possibly due to diluted sediments in the medium. No infectious processes or lesions in the processed material were observed. This is, to the best of our knowledge, the first report of Pestalotiopsis sp; in Perna perna mytilids.
显示更多 [+] 显示较少 [-]Effects of urban heavy metal pollution on organic matter decomposition in Quercus ilex L. woods
1995
Cotrufo, M.F. | Santo, A.V. de | Alfani, A. | Bartoli, G. | Cristofaro, A. de (Dipartimento di Biologia Vegetale, Universita 'Federico II', via Foria, 223-80139 Napoli (Italia))
Anaerobic digestion of spectinomycin mycelial residues pretreated by thermal hydrolysis: removal of spectinomycin and enhancement of biogas production
2020
Song, Siqi | Jiang, Mingye | Yao, Jie | Liu, Huiling | Dai, Xiaohu
Anaerobic digestion (AD) is an effective technology to dispose antibiotic mycelial residues, but biogas production is influenced by hydrolysis rates and antibiotic residue. Herein, the effects of thermal hydrolysis pretreatment for AD of spectinomycin mycelial residues (SMRs) were investigated. The results showed that the removal ratio of spectinomycin was increased while the temperature of pretreatment was escalating. Meanwhile, thermal hydrolysis facilitated the dissolution of organic matters. However, non-biodegradable substances measured by fluorescence excitation–emission matrix accumulated and thus had an adverse influence on biogas production. Based on batch assays, the optimal pretreatment temperature for SMRs was 120 °C. The removal of spectinomycin was benefit for biogas production (increasing by 7.6%), and the overall biogas production increased by 27.6% compared with 289.90 mL gVS⁻¹ of untreated SMRs. The microbial community analysis revealed that spectinomycin (265 mg L⁻¹) might influence bacteria in the early stage of AD (first 5 days), while redundancy analysis showed that spectinomycin had a non-significant influence on community succession over the 32 days of fermentation.
显示更多 [+] 显示较少 [-]