Investigations on the bacterial communities associated to ectomycorrhized roots of seedlings from three stands with different degrees of regeneration decline (high, intermediate and low) and from seedlings grown on monoliths obtained from the very same stands have been carried out. The results suggest that forest decline does not influence bacterial biomass associated to seedlings roots but induce a clustering of specific bacterial species adapted to the different degree of forest decline

Ozone is a well-known phytotoxic agent causing damage to vegetation in parts of North America and Europe. Transboundary or long-range transport of ozone from Western Europe and the generation of ozone from local and regional precursors results in increasing ozone concentrations in Central and Eastern Europe, including the Carpathian Mountaians. Other air pollutants such as sulphur dioxide, nitrogen oxides, ammonia and heavy metals may also affect the health of Carpathian forests. During the 2000-2002 summer seasons, concentrations of ozone, sulphur dioxide, nitrogen oxides and ammonia were monitored with passive samplers. Results of those measurements will be used for development of GIS models of spatial and temporal distribution of air pollutants

Remarkable differences were found in soil development on three sites. At Modru Dul (the least damaged site) Sopdosols and Inceptisols were found, in Alzbetinska (moderately damaged) Entisols are common, while in Mumlavksa hora (highly damaged) the representative soils are several kind of Podzols, with variable degree of drainage restriction and organic soils. The heavy metal content is always rather low and the vertical distribution does not indicate any appreciable contribution of atmospheric contamination

Microbiota in urban green spaces underpin ecosystem services that are essential to environmental health and human wellbeing. However, the factors shaping the microbial communities in urban green spaces, especially those associated with turf grass phyllosphere, remain poorly understood. The lack of this knowledge greatly limits our ability to assess ecological, social and recreational benefits of urban green spaces in the context of global urbanization. In this study, we used amplicon sequencing to characterize soil and grass phyllosphere bacterial communities in 40 urban green spaces and three minimally disturbed national parks in Victoria, Australia. The results indicated that urbanization might have shown different impacts on soil and grass phyllosphere microbial communities. The bacterial diversity in soil but not in grass phyllosphere was significantly higher in urban green spaces than in national parks. Principal coordinate analysis revealed significant differences in the overall patterns of bacterial community composition between urban green spaces and national parks for both soil and grass phyllosphere. Industrial development, as represented by the number of industries in the region, was identified as a key driver shaping the bacterial community profiles in urban green spaces. Variation partitioning analysis suggested that industrial factors together with their interaction with other factors explained 20% and 28% of the variances in soil and grass phyllosphere bacterial communities, respectively. The findings highlight the importance of industrial development in driving the spatial patterns of urban microbiomes, and have important implication for the management of microbiomes in urban green spaces.

Pesticides are used worldwide with potential harmful effects on both fauna and flora. The Kibale National Park in Uganda, a site renowned for its biodiversity is surrounded by tea, banana and eucalyptus plantations as well as maize fields and small farms. We previously showed presence of pesticides with potential endocrine disruptive effects in the vicinity. To further investigate the water pollution linked to agricultural pressure in this protected area, we implemented a complementary monitoring strategy based on: analytical chemistry, effects based methods and the deployment of Polar Organic Chemical Integrative Samplers (POCIS). Chemical analysis of the POCIS extracts revealed the presence of 13 pesticides: carbofuran, DEET, 2.4-D amine, carbaryl, ametryn, isoproturon, metolachlor, terbutryn, dimethoate, imidacloprid, picaridin, thiamethoxam, carbendazim, with the first three being present in the largest quantities. Water samples collected at the POCIS sampling sites exhibited thyroid and estrogen axis disrupting activities in vivo, in addition to developmental and behaviour effects on Xenopus laevis tadpoles model. Based on our observations, for the health of local human and wildlife populations, further monitoring as well as actions to reduce agrochemical use should be considered in the Kibale National Park and in regions exposed to similar conditions.

With the rapid development of aquaculture, the large amounts of pollutants were discharged into the aquatic environment, where the detected antibiotic resistance genes (ARGs) have drawn increasing attention due to their potential threats to ecological environment and human health. Thus, the impact of mariculture on ARGs was assessed and the underlying mechanism of their propagation was explained. Sediments from eight sampling sites were collected along a mariculture drainage ditch, and the sediment in Yellow River Delta National Park was used as a non-mariculture control. Microbial ARGs qPCR array and illumina sequencing of 16S rRNA gene were applied to examine the changing patterns of ARGs and bacterial communities. Results showed that 18 ARGs (3 fluoroquinolone, 1 aminoglycoside, 3 macrolide-lincosamide-streptogramin B, 2 tetracycline, and 9 beta-lactam resistance genes) were influenced by mariculture, and ARGs abundance and diversity were significantly increased in mariculture sediments (p < 0.05). A remarkable shift in bacterial community structure and composition was also observed. The abundance of most of ARGs were significantly decreased in the estuary samples, implying that seawater had a significant dilution effect on the ARGs emission from the mariculture sites. Partial redundancy analysis showed that nutrients, heavy metals, and bacteria communities might directly and indirectly contribute to ARGs propagation, suggesting that the profile and dissemination of ARGs were driven by the combined effects of multiple factors in mariculture-impacted sites.

Total mercury (Hg) concentrations were measured in archived fur from adult female little brown bats sampled at maternity roosts across Atlantic Canada. Mercury concentrations varied significantly among regions and roosts. Bats from Nova Scotia and Newfoundland had the highest median Hg concentrations (9.67 μg/g and 9.51 μg/g) among regions, and individuals from Kejimkujik National Park had the highest Hg (median: 28.38 μg/g) among roosts. Over one third of individuals sampled had fur Hg concentrations exceeding thresholds associated with neurochemical responses. Within-roost examinations of stable carbon and nitrogen isotopes in fur showed inconsistent associations with Hg concentrations. Therefore, the hypothesis that within-roost variation in Hg is driven by variation in diet is not supported by this data, and it is recommended that key prey items be included in future mercury bioaccumulation studies for bats. The elevated mercury fur concentrations for bats from southern Nova Scotia remains an anomaly of concern even when placed in the larger context of Atlantic Canada.

This study assessed concentrations and investigated potential source regions for PCBs, PBDEs, and organochlorine pesticides in Acadia National Park, Maine, USA. Back-trajectories and potential source contribution function (PSCF) values were used to map potential source areas for total-PCBs, BDE-47, and 10 organochlorine pesticides. The constructed PSCF maps showed that ANP receives high pollutant concentrations in air masses that travel along four main pathways: (1) from the SW along the eastern Atlantic seaboard, (2) from the WSW over St. Louis, and Columbus regions, (3) from the west over Chicago, and Toronto regions, and (4) from WNW to NNW over the Great Lakes, and Quebec regions. Transport of all studied pollutants were equally distributed between the first three pathways, with only minor contributions from the last pathway. This study concludes that the high-pollutant concentrations arriving at ANP do not exclusively originate from the major urban centers along the eastern Atlantic seaboard.

45 sites were selected and sampled on the transects and according to the first results of the spatial analysis, 15 more sites were added where useful for the optimal interpolation. Topsoils were sampled including both the soil organic horizons and the more superficial mineral horizon, frequently E, if reached. The depth of the organic horizons, the grass cover, ranked by its hydrophilic character, related to the incipient hydromorphy visible where Norway spruce is more declining, and the Norway spruce health status were systematically recorded

China is one of the largest coastal countries in the world, which have all kinds of marginal systems. Studies have reported the sedimentary Polycyclic aromatic hydrocarbons (PAHs) pollution status, including their concentrations, sources and risks, in localized marginal systems, which showed significant differences. Thus, a comprehensive understanding of their pollution in marginal systems along China Mainland is urgently needed on a national scale. In the present study, the concentrations of 16 priority PAHs in surface sediments from 62 different marginal systems along China Mainland were reviewed. Their sources were identified and apportioned, and the health risks and ecological risks were also evaluated. As a result, the total sedimentary PAHs varied in a wide range of 4–3700 ng/g, with the lowest values observed in Kenting National Park in East China Sea and the highest values observed in Daliao River estuary in Bohai Sea. Their concentrations suggested that they were not contaminated-weakly contaminated in most study areas, but were contaminated-heavily contaminated in some pollution hot-spots. Source identification and apportion suggested that the sedimentary PAHs were mainly originated from coal combustion, vehicular emission, natural gas combustion and petrogenic source, but the coal combustion and vehicular emission contributed most to their emission (>90%). Risk assessment suggested that the carcinogenic risks were lower than the upper limit of the acceptable range (10⁻⁴), which were acceptable at a large spatial scale. However, for sediments from Qinhuangdao coastal wetland, Daliao River estuary and Yangpu Bay, their carcinogenic risks were higher than 10⁻⁴, which will pose high carcinogenic risks for adults. The non-carcinogenic risks were acceptable in all marginal systems with values lower than the safety guideline (<1). In the ecological risk assessment, their concentrations in some pollution hot-spots were higher than the safety guidelines (effects range low, ERL), suggesting a higher potential ecological risk.