Hedges are ubiquitous green elements in many European cities. The selection of hedge types characterized by different traits can be suggested for urban greening projects to decrease pollution levels. At this end, carbon dioxide (CO2) sequestration and noise attenuation capability were analyzed in the following hedge types: Laurus nobilis, Nerium oleander, Pittosporum tobira and Pyracantha coccinea, largely used as green infrastructure in Rome (Italy). Representative hedges for each species were selected from high level traffic streets in the city centre (P sites). Traffic density (TD) was monitored simultaneously with CO2 concentration and noise level (N) in each of the considered P sites. The monthly CO2 sequestration capability (MSC) was calculated multiplying the total photosynthesis per hedge by the total photosynthetic activity time (in hours) per month. The multiple regression analysis predicted noise attenuation (ΔN) by a linear combination of total leaf area (TLA), total leaf density (TLD) and leaf mass area (LMA) of the considered hedge types. All the considered species, being evergreens, were active all year long, including winter, when CO2 emissions from road transport peaked. Nevertheless, among the considered hedge types, P. tobira and L. nobilis were the most efficient species in both MSC (31.6±2.8 and 25.4±2.4 kg CO2 month–1, respectively) and ΔN (15±1%, mean value). The results give insight on the use of hedges to mitigate pollution effects. Moreover, this method can be used to monitor hedge contribution to air quality, in relation to various elements in the city (i.e. traffic density, new cars produced, application of management projects, local laws). These results might be available for projects based on the use of vegetation in order to improve environmental quality in urban areas.

This study explored the interspecies and seasonal variation of polycyclic aromatic hydrocarbons (PAHs) in the extracted lipids of the leaves of seven local plants in an urban environment of Kolkata (22°33′N and 88°20′E), India. Based on the degree of toxicity and carcinogenicity (expressed in terms of their Benzo(a)pyrene equivalent (BaPeq) concentrations) the overall foliar-PAH accumulation during the study period (September 2018‒;August 2019) in the various plants showed the following order: Nerium oleander (80.96 ± 30.08 ng.gdw−1) > Mangifera indica (74.15 ± 20.34 ng.gdw−1) > Lantana aculeata (60.13 ± 21.71 ng.gdw−1) > Thevetia peruviana (40.97 ± 12.45 ng.gdw−1) > Ixora coccinea (38.11 ± 9.5 ng.gdw−1) > Murraya paniculata (37.1 ± 7.35 ng.gdw−1) > Polyalthia longifolia (25.72 ± 5.71 ng.gdw−1). The PAHs like phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, benzo (b+k)fluoranthene, benzo(a)pyrene, benzo [ghi]perylene and indeno [1,2,3-cd]pyrene were predominant during the study period over the PAHs like naphthalene, acynaphthylene, acenaphthene, fluorine and dibenz [a,h]anthracene in the extracted lipids. The temperature-dependent partitioning of the PAHs onto leaf-surface and photo-degradation could have affected the availability of the PAHs. The foliar PAH accumulation varied seasonally as winter (December–February) > postmonsoon (September–November) > premonsoon (March–May) > monsoon (June–August). The leaf epicuticular wax determined the PAH uptake and storage, which in turn was affected by the temperature and solar radiation. In consistence with the idea of “nature-based solutions” for deteriorated air quality remediation in an urban environment, this study could be a promising initiative to build up cost-effective biological filters to combat the airborne pollutants and improve urban air quality.

It is traditionally accepted that urban vegetation contributes to improve air quality by intercepting and retaining the particulate matter. Although the mitigating role of plants has been recognized by several studies, the role of individual species is still poorly understood. This is particularly important in cities like Santiago (Chile), which has high levels of atmospheric particulate and also has high plant species diversity. In this study, we evaluated the retention of atmospheric particles by three widely distributed ornamental species (Nerium oleander, Pittosporum tobira, and Ligustrum lucidum) in Santiago. For this proposal, we took leaf samples in different sampling points across the city which vary in their concentration of atmospheric particulate. Samples were taken 12 and 16 days after a rainfall episode that washed the leaves of plants in the sampling sites. In the laboratory, leaves were washed to recover the surface retained particles that were collected to determine its mass gravimetrically. With this information, we estimated the foliar retention (mass of particulate matter retained in the foliar surface) and daily retention efficiency (mass of particulate matter retained in the foliar surface per day). We found that foliar retention and daily retention efficiency varied significantly between the studied species. The leaves of N. oleander retained 8.2 g m⁻² of particulate matter on average, those of P. tobira 6.1 g m⁻², and those of L. lucidum 3.9 g m⁻²; meanwhile, the daily retention efficiencies of particulate matter were 0.6, 0.4, and 0.3 g m⁻² day⁻¹ for N. oleander, P. tobira, and L. lucidum, respectively. These results suggest that the studied species retain atmospheric particulate matter differentially in Santiago. These results can be attributed to differences on leaf surface characteristics. The recognition of the most efficient species in the retention of the atmospheric particulate matter can help to decide which species can be used to improve the air quality in the city.

In this study, the effects of industrial and urban pollution on Pb, Al, Cd, Cu, and Zn accumulation, peroxidase activity, and pigment and protein contents were investigated in shrub and tree leaves in Antakya, Turkey. We determined that industrial and traffic activities produce the most plant-incorporated air pollutants in Antakya City. Cu and Al amounts were high in plants in the urban street location and Cd, Pb, and Zn amounts where high for all plants in the industrial site. Acer negundo L. showed maximum Pb and Zn accumulation at the industrial site and Al accumulation for the urban street site. Higher Cd and Cu amounts were detected in Platanus orientalis L. and Nerium oleander L. in the industrial and urban street sites, respectively. Compared to the control site, decreases in pigment and total soluble protein contents and increases in peroxidase enzyme activity were more evident in industrial and urban street sites. Our results indicated that industry and urban air pollution is high in Antakya City and Pb pollution was at an especially alarming level for vegetation and human health.

Air pollutant concentration of Trivandrum, the capital of Kerala, exceeded the limits of National Ambient Air Quality (NAAQ) standards, according to a study conducted in 2015 by NATPAC. These polluted corridors harbour vegetation on roadsides and traffic islands, planted solely for aesthetic appeal. Analysis of air pollution tolerance levels of existing plants can act as a scientific basis for efficient planning of the urban landscape. Sixty-seven species, including flowering, fruit-bearing, ornamental, shade-providing and timber-yielding species, were screened for their relative resistance to air pollution. Based on leaf pH, relative water content, chlorophyll and ascorbic acid levels, the Air Pollution Tolerance Indices (APTI) of each species were formulated and they were grouped into the following: tolerant, moderately tolerant, intermediate and sensitive groups. Agave americana (18.40), Cassia roxburghii (17.63), Anacardium occidentale (11.97), Cassia fistula (11.60), Mangifera indica (11.59) and Saraca asoca (10.88) may be considered for planting near green spaces like roundabouts and near pollution prone industrial areas, as they belong to tolerant category. Comparison of APTI during summer and monsoon also revealed the stability of Agave americana, Saraca asoca, Ficus benghalensis, Peltophorum pterocarpum, Ficus elastica, Ixora finlaysoniana, Mangifera indica, Canna indica and Delonix regia in maintaining pollution tolerance even during water disparity. Agave americana, Anacardium occidentale, Ficus elastica, Mangifera indica, Syzygium cumini, Ficus benghalensis, Nerium oleander and Ficus benjamina were found to be suited for mass planting, as was evident from their Anticipated Performance Indices (API).

Multiple drug resistance and increased side effects due to allopathic drugs has warned scientific community with a global alarm to identify molecules from natural sources to combat diseases with minimum or no side effects. The present investigation was aimed to identify and isolate secondary metabolites from traditionally used Nerium indicum using conventional column chromatography which led to the isolation of two compounds, C-I (fractions NB4f1) and C-II (fractions NC13b1). Further characterized, it is elucidated using spectral data and identified as N-(4-hydroxy-phenyl)-2-methoxy-2-phenyl-acetamide, molecular formula C₁₅H₁₅NO₃, and molecular weight 257.3 (C-I) and N-(4-hydroxy-phenyl)-2-phenyl-N-phenylacetyl-acetamide, molecular formula C₂₂H₁₉NO₃, and molecular weight 345.4 (C-II). Further, the isolated compounds were investigated using in silico approach by Autodock tool with four different proteins specific for cancer and in vitro assessed cell proliferation, and apoptosis against human breast cancer MCF 7 cell line. The results of the in silico model demonstrated potent binding affinity of both compounds with the proteins representing that the isolated molecules could be a drug of choice for cancer. Further, the isolated compounds revealed significant inhibition of cell proliferation (IC₅₀ values 21 μg/mL for C-I, 19 μg/mL for C-II) with induced apoptosis with nuclear condensation effect on the MCF 7 cells in in vitro condition even at very low concentration. Compound treatment to MCF-7 cell line represented bright fetches indicating condensed chromatins and higher level of nuclear fragmentation with DAPI staining, indicating higher cell death due to induced apoptosis and confirmed using flow cytometry analysis representing inhibition of cell proliferation at S phase. Graphical abstract

Air pollution due to vehicular emissions has become one of the most serious problems in the whole world and has resulted in huge threat to both the environment and the health of living organisms (plants, humans, animals, microorganisms). Plants growing along the roadsides get affected at the maximum as they are the primary recipients to different air pollutants and show varied levels of tolerance and sensitivity. Taking this into account, the present work was based on assessment of seasonal variation in air pollution tolerance index (APTI) and anticipated performance index (API) of four roadside plants, namely, Alstonia scholaris, Nerium oleander, Tabernaemontana coronaria, and Thevetia peruviana belonging to family Apocynaceae. APTI was calculated by the determination of four important biochemical parameters, viz., pH, relative water content (RWC), total chlorophyll (TChl), and ascorbic acid (AsA) content of leaves. The leaf samples were collected from plants growing at seven different sites of Amritsar (Punjab), India, for pre-monsoon and post-monsoon seasons. Highest APTI (82.14) was reported in N. oleander during the pre-monsoon season while the lowest was recorded in T. coronaria (18.59) in the post-monsoon season. On the basis of API score, A. scholaris was anticipated to be an excellent performer during the pre-monsoon and post-monsoon seasons followed by N. oleander, T. coronaria, and T. peruviana. Linear regression analysis and Pearson’s correlation coefficient depicted significant positive correlation between APTI and ascorbic acid content during the pre-monsoon and post-monsoon seasons.

The analysis of the airborne particulate matter—PM—incorporated to plant leaves may be informative of the air pollution in the surroundings, allowing their use as biomonitoring tools. Regarding metals, their accumulation in leaves can be the result of both atmospheric incorporation of metallic PM on aboveground plant organs and root uptake of soluble metals. In this study, the use of Nerium oleander leaves as a biomonitoring tool for metallic airborne pollution has been assessed. The metal uptake in N. oleander was assessed as follows: (a) for radicular uptake by irrigation with airborne metals as Pb, Cd, Cr, Ni, As, Ce and Zn (alone and in mixture) and (b) for direct leave exposure to urban PM. Plants showed a high resistance against the toxicity of metals under both single and multiple metal exposures. Except for Zn, the low values of translocation and bioaccumulation factors confirmed the excluder behaviour of N. oleander with respect to the metals provided by the irrigation. For metal uptake from airborne pollution, young plants grown under controlled conditions were deployed during 42 days in locations of the city of Zaragoza (700,000 h, NE Spain), differing in their level of traffic density. Samples of PM2.5 particles and the leaves of N. oleander were simultaneously collected weekly. High correlations in Pb concentrations were found between leaves and PM2.5; in a lesser extent, correlations were also found for Fe, Zn and Ti. Scanning electron microscopy showed the capture of airborne pollution particles in the large and abundant substomatal chambers of N. oleander leaves. Altogether, results indicate that N. Oleander, as a metal resistant plant by metal exclusion, is a suitable candidate as a biomonitoring tool for airborne metal pollution in urban areas.

The vegetation plays important role in urban environment and associated ecology. Urban vegetation experienced various changes due to biotic and natural interference which alter the vegetation structure, function and ecology. The present study deals with the assessment of shrub species structure, diversity, biomass, volume, C storage and CO₂ mitigation potential in an urban setup. Total four sites were selected, viz., east, west, north and south directions of Ambikapur City. A sum of eight shrub species representing seven families was recorded in Ambikapur City area. The highest representatives were recorded towards east direction (seven species with six families) and lowest towards south direction (five species with five families). The total density of shrubs ranged between 230 and 570 individual ha⁻ ¹ being highest at east direction and least at south direction. The higher diversity was found at east direction. The shrub biomass was ranged between 0.999 and 2.603 t/ha being highest at north direction and lowest at east direction. The shrub volume, C storage and CO₂ mitigation values reflected similar trend as in case of shrub biomass. The shrub volume, C stock and CO₂ mitigation values were ranged from 0.141 to 0.250 m³/ha, 0.437 to1.132 t/ha and 1.597 to 4.156 t/ha, respectively, among the various sites. The species like Cestrum nocturnum and Nerium oleander are found to be potential in terms of various ecological services such as biomass, C storage and CO₂ mitigation in different sites. However, Lantana camara was also found to be potential species under urban setup which can be utilized for its various ecological functions. Further, it was found that the contribution of the non-native species was higher over native species in terms of stand density, basal area, biomass, C stock, volume and CO₂ mitigation potential among different study sites.

Nursery grown plants of Nerium oleander, Pogonatherum crinitum, and Portulaca oleracea were observed to remove fluoride up to 92, 80, and 73%, respectively, from NaF solution at the concentration of 10 mg L⁻¹ within 15 days. Concentration range of 10–50 mg L⁻¹ of fluoride revealed a constant decrease of removal from 92 to 51% within 15 days by N. oleander, while the biomass (one to five plants) showed enhancement in removal from 74 to 98% in 10 days. Translocation and bioaccumulation factors calculated after fluoride contents in roots and leaves of N. oleander, P. crinitum, and P. oleracea were 1.85, 1.19, and 1.43, and 9.8, 3.6, and 2.2, respectively. P . oleracea, P. crinitum, and N. oleander showed reductions in chlorophyll contents by 40, 57 and 25 and 8%, carbohydrates by 50, 44, and 16%, and proteins by 38, 53, and 15%, respectively. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) in the roots of P. oleracea, P. crinitum, and N. oleander were observed to be induced by 400, 383, and 500%; 80, 105, and 424%; and 153, 77, and 71%, respectively, while the leaves showed induction in SOD, CAT, and GPX activities by 550, 315, and 165%; 196, 227, and 243%; and 280, 242, and 184%, respectively. Results endorsed the superiority of N. oleander for fluoride removal over other plant species.