In most developing nations, municipal wastewater treatment is limited to aerobic secondary treatments, expensive and ineffective in removing nutrients from treated effluents before discharge, resulting in eutrophication and imbalance in receiving bodies. As a result, the effectiveness of Chlorella vulgaris for primarily treated wastewater collected from a sewage treatment plant during an 8-hour detention time was investigated in this study. Microalgae have been found to efficiently remove organics and nutrients to levels far below the desired limit in the present research. After algal treatment concentration of COD, phosphate and ammonia reduced to 12.43 mg/L (93.75%), 0.04 mg/L (98.40%) and below detectable limit (100%) respectively. In addition, remarkable reduction was found in solids (TSS, TS and TDS) and EC concentration. The use of microalgae resulted in an increase in DO concentration. As a result, introducing Chlorella vulgaris into a wastewater treatment system can lower nutrient and organics contents without any additional treatment.

In developing countries, wastewater treatment is confined to secondary systems. Hence even after treatment, wastewater effluent has a high level of nutrients which causes eutrophication and has destructive impacts on receiving bodies. Literature reveals that phycoremediation can be the best solution to address the problem faced but is time-consuming, ranging from days to weeks. Hence, the present study aimed to determine an optimum detention time for the microalgal system to treat domestic wastewater. The retention time for treatment in the study was divided into an aeration and settling periods. During the study, aeration time varied from 2 hours to 24 hours, followed by 1-hour settling period for each aeration time. Optimum detention time for microalgal treatment was obtained at 11 hours of detention time (10 hours aeration and 1-hour settling). Parameters analyzed during the study were pH, EC, TS, TSS, TDS, nitrate, phosphate, ammonia, COD and DO. However, the main focus was on nutrients (phosphate and ammonia) and organics (COD) removal while determining the optimum detention time. Maximum removal efficiency obtained for COD, ammonia and phosphate for non-filtered effluent was 75.61%, 90.63% and 83.29%, respectively. However, removal efficiency further increased for filtered effluents to 86.34%, 100% and 91.12% for COD, ammonia and phosphate, respectively. Algal treatment offers an ecologically safe and more affordable system for nutrient removal and eliminates the need for tertiary treatment.

International audience | Growth in the presence of sucrose was shown to confer to Arabidopsis thaliana (thale cress or mustard weed) seedlings, under conditions of in vitro culture, a high level of tolerance to the herbicide atrazine and to other photosynthesis inhibitors. This tolerance was associated with root-to-shoot transfer and accumulation of atrazine in shoots, which resulted in significant decrease of herbicide levels in the growth medium. In soil microcosms, application of exogenous sucrose was found to confer tolerance and capacity to accumulate atrazine in Arabidopsis thaliana plants grown on atrazine-contaminated soil, and resulted in enhanced decontamination of the soil. Application of sucrose to plants grown on herbicide-polluted soil, which increases plant tolerance and xenobiotic absorption, thus appears to be potentially useful for phytoremediation.

International audience

Stimulation of photosynthesis by elevated CO2 has been consistently found for aspen but not for maple. Similar responses have been shown for growth. In contrast, O3 causes decreased levels of photosynthesis and growth in aspen but does not appear to impact sugar maple significantly. When the pollutants co-occur, CO2 induced enhancements in photosynthesis and growth are moderated so that trees in CO2 and O3 treatments respond similarly to those in control rings. In this presentation, we will provide a physiological interpretation of our results in modelling growth response under future atmospheric conditions

Under elevated CO2 photosynthesis was 15-50% higher than in chamber controls depending on the weather conditions of the growing seasons. When measured at 360 ppm CO2 both elevated CO2 and elevated CO2 + O3 treatments decreased net photosynthesis, stomatal conductance and also transpiration, indicating downregulation of photosynthesis at elevated CO2

Plant-derived saponins are bioactive surfactant compounds that can solubilize organic pollutants in environmental matrices, thereby facilitating pollutant remediation. Externally applied saponin has potential to enhance total petroleum hydrocarbon (TPH) biodegradation in the root zone (rhizosphere) of wild plants, but the associated mechanisms are not well understood. For the first time, this study evaluated a triterpenoid saponin (from red ash leaves, Alphitonia excelsa) in comparison to a synthetic surfactant (Triton X-100) for their effects on plant growth and biodegradation of TPH in the rhizosphere of two native wild species (a grass, Chloris truncata, and a shrub, Hakea prostrata). The addition of Triton X-100 at the highest level (1000 mg/kg) in the polluted soil significantly hindered the plant growth (reduced plant biomass and photosynthesis) and associated rhizosphere microbial activity in both the studied plants. Therefore, TPH removal in the rhizosphere of both plant species treated with the synthetic surfactant was not enhanced (at the lower level, 500 mg/kg soil) and even slightly decreased (at the highest level) compared to that in the surfactant-free (control) treatment. By contrast, TPH removal was significantly increased with saponin application (up to 60% in C. truncata at 1000 mg/kg due to enhanced plant growth and associated rhizosphere microbial activity). No significant difference was observed between the two saponin application levels. Dehydrogenase activity positively correlated with TPH removal (p < 0.001) and thus this parameter could be used as an indicator to predict the rhizoremediation efficiency. This work indicates that saponin-amended rhizoremediation could be an environmentally friendly and effective biological approach to remediate TPH-polluted soils. It was clear that the enhanced plant growth and rhizosphere microbial activity played a crucial role in TPH rhizoremediation efficiency. The saponin-induced molecular processes that promoted plant growth and soil microbial activity in the rhizosphere warrant further studies.